TOXICOLOGY

• Document Type: CREST
• Collection: General CIA Records
• Document Number (FOIA) /ESDN (CREST): CIA-RDP80-00809A000600200035-2
• Release Decision: RIPPUB
• Original Classification: R
• Document Page Count: 4
• Document Creation Date: December 22, 2016
• Document Release Date: June 29, 2011
• Sequence Number: 35
• Publication Date: May 20, 1948
• Content Type: REPORT

Sanitized Copy Approved for Release 2011/06/29: CIA-RDP80-00809A000600200035-2
FOD
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PLACE
ACQUIRED
DATE OF
3H$CHUATIOH
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(LISTCD Cmw'"9)
SUPPLEMENT TO
REPORT II0.
STAT
STAT
"mamma" cewwm5Iwmnwan_ww.rrean n~_ I T01uttwct
IlMnrA1 IIATCn lmrnaMaT)(1U MP THE RESFARCI'I
THIS IS
V. S. C.. 3) "D U. Al "='1.w m iRMm:cucx ux .na ~.vL ;:
pwp~e~ppen rnwrurw ew ur mmwc m rw uw~onw,r e. rC??w n ao USE OF TRAINED IVTELLIGENrE ANALYST'SPoOYC01 1?e ,N' "AV e vimui=0
SOURCE Rusaien periodieaa, Ht lefion T:.Ico~eriaaeet ll`r Ery~Jlc L R I~c~i~ J_nY,
Vol "Us 110 3, 1947, ?patihogenaois of Botulism, 1V: Reaction of
Vascular Tissues of Humane and Animals to 3otuliemotcxin~" i-F98--Par
{ - ?-- Translation ..pocii`_celly ,educated,)
RE angI C VA...ma R TISSUFrS OF Hi1NAH?~t?I` ANTMt_:LS T BC, 1?LY0SitZ3iSI
K. I, iAatveynv, Institute of Mtcrobioloa, Epidimology, and Con-
tagious Disenoos, Acadamy of Medical S::iencea of the USSR and
the Chair of !Siorobiology, Second Medice1 Iunti.,ute ir.tr,i I,. V,
Ll
oacaw.
Stalin,
(Submitted to the editor, k '!s:c:?c 190;
be .'!' n t nt 1i nn_t nn i r: hUm ana and .3t '[sa1D -a ri'~t.ll
'u3w~ ..~,eu~-,aao. ,._., cf ....
uio not clear, in spi.o of a considerable amount of work which he., b^en corn on
the subject (Deck, rack and Hoskins, Wood, Fridman and lo.bor, Leynbs,g,
Deteygin and Khayfets, Pctrevskiy, Nnumenko cmd Batu_?iinko, ScrebryannaYa
and Shkavera, and othora),
eriee'ntal research conducted to detarmiae the rccurcy of
lt
f
R
s o
e::p
esu
the hypothesis of clinicians on the vascular contractive action of botutismo-
toxin and presented in the Present TTork..
Experiments i''ith vessels o. human kidneys, rabbi., ear, ':id ",.be !,!nd
half of guinea pigs were carried out according to the Kxrv1 oT -?is: mrkI r
method, Td1ich user puretcxin. No use of such a to..in produced core rafinite
results co.noerniq; tic res::au of the A dry toxin of type: 1. or B botulie t brutlli was prepatee by reci.pit4-
tion in nn 3uanon5v,1 au1p's to solution. 11V toxin fwosting-on the sturz:zce of
the medium :van eo:_Teeted n e cup and carefully ?reased to renovo s+oi :t, ref
Then the toxin war dried, pu;v riled info fine po3de;? in a ca:rta,:?_ anc
titrstea to determine ito viruleooe.
A toxin of c gsidnrabie toxicity used in the exoe:ac^eLts. O,C :UOI
to 0,0000001 g lethal ;'a; mica. iho ''r;+ toxin n_'3 d5.les;e 1U:, tia.aa
?.... - -
CLASSIFICATION 'fCTED
STATE NRVY 'X p5_ ~ D15iR18UTION
I.ARItY
K-A
D;T_D `~ .
INC. CF P,tCEi?
Sanitized Copy Approved for Release 2011/06/29: CIA-RDP80-00809A000600200035-2
 Sanitized Copy Approved for Release 2011/06/29: CIA-RDP80-00809A000600200035-2
and then subjected to dialysis through a collodion bag for 16 - 18 hours
in running :ap water, and then for 24 hours in distilled rater.
?ruith this method it was possible to obtain a toxin free from the
mar>+' impurities which are found in a liquid toxin. The toxin which we
tbtairied contained almost no amines which did not precipitate in anno-
niumsulphate solution. In addition, At was freed from some albumins in
the medium which had not precipitated?in the uuncentrated ammonium sul-
phate solution. with dialyeip, several impurities of various salts and
possibly many other substances were removed.
After dialysis the toxicity of the solution decreased 2 - 3 times.
Apparently, this was due to partial destruction and. dissipation of the
toxin during dislysis. Accordingly, solution:r with high toxicity wore
used in the experiments.
gxperiments with the ears of 15 rabbit were carried out in the
first series. Previously, the artery in the oars of the rabbits was
separated and tied with.two ligatures. Than the ear way incised with e.
sharp razor between the two ligatures. The vessels of the ear worn
washed with a wane 8'inger Locke solution. iyhen.th4r, constant tone
was established, w}?.ieh wwa~ dotermiised by the c;uurati2,y of liquid discharged
in one miirnit% we introdsbsd first a weak and then a stronger conoen-
tration of one toxin. The first toxic. di'utien was 1:50,000; the sec-
ond was 1:25,000; and the third was 1:10,000.
The ordinary solution of the toxin was i:;troduoed in one ear of
6 rabbits of this aortas. tanda aalutten o: attenuated toxin tnto the
others. A toxin dilution of 100 times was attan::cbsc?bj !,r. a
water bath after dialysis for 15 - 20 m nutes..
The vessels in the ears of all 15 rabbits reacted to the bdtulis--
motoxin and contracted (see table).
The vessels had an average contraction of 23.Z percent with the
first dilution, 36.3 percent with the second dilution, and 36.9, per-
cent with the third. The vowels of the rabbi'. e& u a aotsd almost i-
dentically to the second and third dilution or the toxin.
Vessels of the ears of the 6 rabbits. through which the attsnc-
ated toxin had beet introdueedahad an insignificant contraction. The
average contraction with the Sint dilution was 6 percent, and with the
second and third dilutions, 6 and 6.5 percent,
Very definite results of the reaction of the vessels in the rab-
b+_t ears to botulismatmzin were obtained in these axpvrinnnts: the
higher the concentration of the toxin, the stronger the contraction of
the vessels. Botulismotosin which had been attenuated by heating, then
purified by preliminary sedimentation and subjected to dialysis, oauoed
a very slight contraction of the vessels in th. rabbit ears, espoci.U;..
in'comparisom with natural toxin,
STAT
Sanitized Copy Approved for Release 2011/06/29: CIA-RDP80-00809A000600200035-2
 Sanitized Copy Approved for Release 2011/06/29: CIA-RDP80-00809A000600200035-2
STAT
Hev..ction, of Blood Ve:isela cr P.n-iwls and Husan:3 to 5otuliSaOE0Aif1
F:xp of
ear Type of vessel erp
r.v decrease of +w
drops in paosage of
of diluted toxin exp
i Rabbit Kara 15 232 36.5 )6:9. 6 4 6 6,5
2 Guinea Pigs 10 23,1 25.2 't5.6 5 4.6 6.4 3'g
3 Hue. n Kidneys 10 22.1, 25.; :s2 ' 3 4.9 7.4 1617
The serondserios Of experiments was with 15 &Unea pigs (ace table),
The guinea pigs were killel rith aJblow on the head. The abdominal cav-
and t on tLe
f
-
P
-
. gla-- -?-????---- .
-
guinea pigs cut in hall with sharp :3cioovroe
into the aorta of the hind half of the guinr.e p3RsYendthe~vninsQirrigated
examined
until a
h
r-----
v-&'
on a slide and again a Ringer-.Locke ac .lion woo ri-
was reached where a constant volume of liquid flowed out per adnute.
Identiodt dilutione?of toxin as in the previous series were studied
essels of 1V, guinea pigs were tested with a toxin
ti
f
v
on o
The reac
here. which had not been heated. Duo to the pasting of the first and second dilu-
traction
e co
n
tions of the t orin, the vessels of the animals h:d an averag
of 23.1 - 25.2 percent, and with the third dU.u ion, 35.8 portent,, Toxin
auiw.. ba, w^..n at-?,t_ _te_ d by heatingca..aed Y contraction of vessels of
. u a.. ..t
guinea Lisa of 4.2 .`? d.
The results of the experiments with guinea pig vessels did not differ
from the results of experiments of the first series, which ware made with
uinea pig vee,els reacted with a considerable
The
l
g
rabbit ear vesse
s. contrectit:n to the botu1ismomixin and showed aluast no reaction to the tox-
in which had been attenuated by heating.
The reaction of vessels of human kidneys to botuliemotoxia was stud-
ied in the third series.
In order to obta'.n definite reeulta?, M. was vary important to use (rash
-rgans from persons not suffering from vascular disease, 'hich was determined
by autopsy, lie succeeded in getting organs fo the mot part within 4 ~ 6
aoure after death.
t, A total of 13 experiments were with sidnotys. Tue t.,atihcd wis the cape
p;s in previous experiments. A glass cannu3a was introduceu into the artery of
the kidney and a warm Ringer-Locke solution run through it until the solution
contained no Llood. After this a solution of botulismotoxin of the same series
and the same dilution ai used in the previou:-i expor es;ts was rue through the
vessels of the kidney,
Sanitized Copy Approved for Release 2011/06/29: CIA-RDP80-00809A000600200035-2
Av decrease of
drops in paeoage
of, diluted toxin
 Sanitized Copy Approved for Release 2011/06/29: CIA-RDP80-00809A000600200035-2
With the first dilution of the toxin, the vessels of ten human kidneys
had an average contraction of 22