IMMUNOLOGY

• Document Type: CREST
• Collection: General CIA Records
• Document Number (FOIA) /ESDN (CREST): CIA-RDP80-00809A000600200141-4
• Release Decision: RIPPUB
• Original Classification: R
• Document Page Count: 5
• Document Creation Date: December 22, 2016
• Document Release Date: June 29, 2011
• Sequence Number: 141
• Publication Date: July 13, 1948
• Content Type: REPORT

CLAS$ AT!orj I;ESTRIC7y
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'THIS IS UNEVALUATED INFORMATION FOR THE RESEARCH
USE OF TRAINED INTELLIGENCE ANALYSTS
SOURCE Aneaian psriodloal, nlletenr Ekeuerisental'n iolo~ii i Medi- beiav
'rot 23, 90 6, 1947? FDB Per Abe 1 T10~-- Trer.elntion epool
fical.lq re-
quested.)
IMW3O=NIOrrY OF BRUCELIA ABORTUS 19 STRAIN
0 TIM ON OUINEA PIGS AND HUMAN DEIN06 -- PRBLIIMAARF REPORT
!Tore the Sector of Infectious Pathology (Read -- Active Member of
the Ao.Ae.
f v_" --? -
y o
-- - ~ vaww+uva &a no 1mmunlsation of guinea )i
'
ehoen,
hp i~r, that on the average only iO ,.erc
r
ui ed
r
.
uc.a.a aeticenele ibrectioa. We furthermhre eetab-
11hhed that such Qoenired i?nnie. n..A-- th--- ---
a teg?e v1ra1.ma Goss of Bruoella showed
70-80 pereect reeiatant +isd those given 5 to 10 virulent doses 40 percent re-
sistaat;whereas If those injected with 50 virulent doses, 95 percent becano
infected.
Pbrthei9ore, the lsanaity of inoculated
i
gu
nea pigs is of short dura-
tion. The Camber, Of these animals retaining immunity decreased to one-third
r'u1Kft the 3 a nthe afte
i
r
nceulat'on. Consequently. inoculation with 'rill-
6d veucines did not prove suneenafn7- K
ve--
o
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male by immunising them with aviralent living Brucelia sate 22 vaccines.
The imsuno?ssioity in these animals was considerably greater, and 35 per-
cent remained immune even after 50 virulent doses of Aruceila were in-
jected.
TED
Having obtained these encouraging results with the avtrulent strain
of Brucella outs 22, we then took up the American strain, Brnoella abortus
19, which to widely used in immunising domestic animals.
We married out the *o1loving tests in ooncection with this strain:
1. Determination of the virulence of this strain in guinea,pigs.
2. Determination of the retention period of this strain in,the tie-
ones of the animals.
3. Determination of the iamunogeutolty of this stra'o in guises pigs
and also a tentative determination of the tolerance and immunogentaity of
this strain in human beings.
Guinea pigs weighing 350-400 grams were subcutaneously iujooted with
a 48-hcnr culture of Brucella abortus 19 In doses *out-aioing from 1,000 to
2 billion bacterial count (souoraiog to Ih' microscopic _tradard of Tc KE).
These inoculated aoimalu were killed 30 dame later and cultures were made
from tissue specimens of the lfuphatio glands, groin, parotid gland, msui!-
la and cervical fool; and also t n' tbn ep17Nh, divers blo;vi, arias, and
marrow. Results of these cultures are shown in Table 1.
Doses
No of
Cultural Resulte
Guinea Pigs
Positive
Negative
.,000
6
0
6
100,000
4
4
0
1,000,000
2
2
0
100,000,000
2
2
0
1,000,000'
6
6
0
2,000,000,000
5
5
0
? jtc; probably should be 1,000,00o,oopJ
Brucellm abortus l strain above lass virulence in guinea pigs. Now.
ever, its "residual" viv"lance romaine sufficiently ap *zent insofar as a
dose totalling 100,000 bacterial count produ:eda gsnerelised infection in
those,aaimale. It is also Interesting to note that we failed to observe
either clinical or pathological degeneratior.s in those animals injected
with Brunelle, abortus 19 strain.
strain culture containing a dose of one billion baeterte count. Path-logic
aeatpoW aM bacteriological etam!oatton of tbeec animals were wide at sari-
ous intervals after the injection. Results of these es-mtoati.ooe are shown
in Table 2.
STAT;
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In days In months
3-5 5-10 15-20 30-35 2 3 5 6
Time of
Pathologic Anatomy
M
No of guinea pigs examined 5/5 6/6 19/19 7/6 5c/24 21/5 19/2 29/1
Percent of cultures
obtained 100.0 100.0 100.0 85.0 48.0 23.8 10.5 3.5
NODE: Numerator shows number of guinea pigs examined; denominator shove.
number of cultures obtained.
wire insignificant and were only in isolated colonies on agar. Cultures
often showed propagation to broth only, i.e., due to increased nutritive
value.
0. teoun aoaa'-wits ofBracells Aborrttue 3.9 Vaccine in inem Pisa
killed 2-3 months after the injection, but after 6 months it was possible
to obtain cultures only from the urine specimens of one animal cut of 29.
These experiments indicated in this manner that a certain number of
these animals shoved signs of recovery from Brucella abortus 19 infection
in about 30 days and to 6 months they became practically immune to Bruoel-
loeis. Cultures were made generally from lymph fact to animals which were
to this injeetion, the animals showed iaannobioloog$lical reactions (Wright
reaction. opeonio, ph gocytia, and allergic tests). These teanieed ant
Guinea pigs were oubeutaneouely infected with living BraoeUa abort
us :9 vaooi`e with a does containing a one billion bacterial count. Due
. udder of an aborted sheep.
The following pathologic anatomy and bacteriological analyser of the
animals in all the taupe were made 30-35 days after the injection of the
virulent strain. Cultnree from each organ wezA differentiated according
to their forwtior of a?s and/or producing aniline in the culture media.
group was injected with one does 2 month.' after iemuntsetioa. Finally, 4
aoatbe after immunisation, c. fourth group was injected with killed vaccine
having a 2 billion bacterial count. Thirty days after the seaood injection
these naimale were injected wits a bigbly virulent strain isolated from the
After 30 days, the first group of Immunised animals was injected with
one does of Brucells. 4u the second vitro five dopes. 8laultaaenuely, con-
trot animals were injected with identical doses of Brucelle. The third
aftow the original vaecinatian.
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fem.. -.~
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RE?,TRIOM
Table.3
tion Appeared munity to in. quired im+nun-
lie of Ant of No of Days No of No of Animals No of Nco-
Inoculated Virulent after Inooula- Animals Infected With Infected
Animals Dose tion Before Infected Br. Abortue 19 Animale
Stone of Infeo- (showing in- (nhovieg so-
14
1
30
0 9
1B
5
30
0 9
21
1
60
3
Revaooinated
animals
29
1
150
42
Control
1-5
Table 3 above that anieele inoculated vith Brucella abortue 19 vaccine
were 100 percent isuaude to Brucelie melitadoie after a acot,h and 50-94 per-
cent were due to acquired immaity. Those ant' Ls which caved no trace of
t) soon after iaoculat'.on o ver-
0
n
(
en
par
35-5
Brunelle aboztus 19 vaccine
theless showed no slag of infection and apparently were in a period of ac-
quired tsuunIty. Two months after ineculAtion 94 percent of the aniwkle
against Bruoella meliteasie, and the majorityc~f
nit
d i
y
san
already showe
these (ssbout 64 peroenty wed no iofegtiou. However, 14 percent of the
cni*ale have apparently lost thoix aoquirod 1uu.nLLy by this tine. Fioaliy,
revaccination tests proe4A that it wee simple to "ri~etivate" acquired
imuanity in 4 aaoathe after Use first inoculation by revaco'_nation with
uthled vue.-Inc. 8inety-sever. percent a" the animals acquired resistcace
d of revs oainatton.
th
h
o
ie me
to virulent Brucelle 16Jootiona through t
The results of the iosuoologioal reactions in animals inoculated with
Bruoella abortus 19 vaccine are shown to Table 4.
Table 4
Time After Avg Titer for Wrtoght
Inoculation Reaction Test
.3 64a
60 days
4 months
Avg Index of 0080010
phsgaoytic Reactions
1t290 40
1:40 27 67
Table 4 shows a considerable decrease in the agglutination titer and
also the. index of opeonio phagoeytio reaction in the animals after 4 re.,"ths.
On the other hand, the Biurne reaction shows an increase tendency which
D. O tt,-gf gesso Reims with Brdoella Abortta 19 Strain
At the and of last year (1946), we inoculated a few volunteers with
living Braoall& abo^tuu 19 vaccine after testing.thie vaccine on guinea
pigs. We chose volunteers who were negative to Braoel::~ste (Writ::.. Sud-
dslsoa, opson!o phaSocytiC, and Biurne reaction testa) and frrt of other
undesirable ttsetore to hinder this inoculation test. These men ranged
frog 23 to 43 yearr. The vaccine vac preyared from a 48-hour culture of
STAT
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Bruoolla abortue 19 in physiologic salt solution. A 1-cc dnae`van in
F had 3T.1 in 36 hours. On the injection day and the following days these
Both showed insignificant Local reactions and there was slight iaflamaa-
f:tnn to the tnianted sites after 24 hours with very little pain lasting
daces fora guinea pig whiLe subject V was injected with twice this amount.
determine the tentative immunogenic doses of Bruoella abortue 19 vaccine
for human beings. As an example, we could mention the following data
obtained from two of the volunteers (K and V) who were inoculated with
various doses of Brucolla abortue 19 vanoine.
Subject S was injected with a does equivalent to 1,000 virulent
and general reactions, as well as the iemunooiotogioaL roacbL1Jcw .n-
studied, in addition to the careful observation of the general condition
below fever level within 19-21 days after inoculation with a maxlesaa of
3T.3 and remained at this level for 23-25 days. Both subjects were feeling
is.06uisttin. Finally, is the course of observation of the general health
So
titration, and a strong positive Biurne reaction. ?or the same per ,
showed positive Waght and 8addelson remotion* in 1:200 titration after
Me double dose of vaccine, and positive Blurne rerotion also. Further
study of ismnnobtologionl reactions of these inoculated volunteare shooed
that they respondedto seroingioal ar0 allergic taste two months after
ne;tive Wright reaction, but, the Buddelson reaction was well. defined In
Ls?00. Blued cultures wars nepitive. K had a positive Wright reaction
In X00 titration after 30 days, and positive Huddelson reaction is L:200
d F
the following results
Study of the immunobiologioal reactions in inoculated emus determined
geaioity.
far as 'atom betuge were concerned, and indicated apparently good immuno-
The yxsllstoery experiments on mean beings have shown io this manner
gaibe we J..
Thos, the 7irst tentative experiment warrants a continuation ;,f this
work in order to determine ooocluaivals, tho offectiveneen of inoculation
-5-
RBSR.ICTSD
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men performed their full quota of work as usual.