UTILIZATION OF THE BLOOD OF AGRICULTURAL ANIMALS: V. NITROGEN METABOLISM IN DOGS WHEN SPECIES NONSPECIFIC SERUM IS INTRODUCED INTRAVENOUSLY

• Document Type: CREST
• Collection: General CIA Records
• Document Number (FOIA) /ESDN (CREST): CIA-RDP80-00809A000600370217-0
• Release Decision: RIPPUB
• Original Classification: C
• Document Page Count: 6
• Document Creation Date: December 22, 2016
• Document Release Date: September 27, 2011
• Sequence Number: 217
• Publication Date: January 4, 1951
• Content Type: REPORT

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~~~~~~ONFIDENTIAL
CENTRAL INTELLIGENCE AGENCY REPbR'.'
INFORMATION FROM
FOREIGN DOCUMENTS OR RADIO BROADCASTS CD NO.
COUNTRY USSR
SUBJECT Scientific'- Medicine, species nonspecific
serum
HOW
PUBLISHED Monti7.y periodical
WHERE
PUBL"1SHED Moscow
DATE
PUBLISHED dan 1950
LANGUAGE Russian
TXIS OOCV YIN! CONTAIN! IN lONYA710N Af}tGTIN{ 1Nl NATIONAL Dt-tll{t
Of TNt UNITlO {TAtt3 NITNIN iM[ Y[ANIN{ 0/ [3-IO NA{[ ACT t0
y. {, G., 11 AND }i. A{ AYlND[Y. ITS THAN{YI3310N ON 7X[ [[Y [CATION
Of ITS CO Nil NT3 IN ANT YAN NI! f0 AN VNAU7X 0{12[G }[[{ON 13 }AO?
MI{IT[D {T LAN. NI}{ODU OTION O/ 7X13 CONY I{ }ROMI{Iii D. J
DATE OF
INFORMATION 1950
DATE DIST. ~ Jan 1951
NO. OF PAGES 6
SUPPLEMENT TO
REPORT
THIS IS UNEVALUATED INFORMATION
Doklady Vsesoyuznoy Ordena Lenina Akademii Sel'skokhoz tvenn kh Nauk
imeni V I Lenina, Vol XV, No 1, 1950, pp 1 -may.
UTILIZATION OF THE BLOOD OF AGRICULTURAL ANIldAI16:f
V. NITROGEN METABOLISM IN DOGS WHEN SPECIES
NONSPECIFIC SERUM IS INTRODUCID INTRAVENOUSLY;~'?
V. V. Npvosel'skays
Moscow Chem-Tech Inst of Meat Industry
Submitted l Dec 1949
his report gives additional information on the properties and '
applications of species nonspecific serum (VNS) prepared from the
blood serum of cattle. The use of VNS serum for blood transfusions
and for supplying parenterally additional protein nutrition in both
human and veterinary therapy has been proposed_7
TYie st~idy of the physicochemical properties of species nonspeclfic serum,
proposed by Academician N. G?. Selen'kiy, showed th9.t it containp,Yr'om 5.5 to
8.2'~i protein and that the amino?,apid cflmposition of this protein is~?very similar
to that cif the protein of.hutuan whole blood. It was also shown,tbat VNS can be
introduced into the blood stream of man and animals where it pi?oducea a very favor-
able eYfect similar to that of homogeneous plasma or serum. Since this was of
great interest, a detailed. study was proposed of the nitrogen metabolism when
species nonspecific serum?is introduced intravenously to show the serum's properties
as a source of protein nutrition for the body.
To make this study, we set up, under the direction of Academician N. G. Be-
len'kiy, a aeries of e~per~meflts with d.ogs.? The present article outlines the met-
hod and most typical tests used. '
Each group of testa roias preceded by a preliminary adaptation period (approx-
imately 8 days) of feeding the test anima.'_a a definite food ration. The preliminary
period load to last at least 3 days to establisb. a condition approaching nitrogen
equilibrium, and to accustom the animals to the conditions of the test.
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~~~F9DE~1~'IA~
Throughout the course of the teat, the amount of protein in the food was
constantly checked (nitrogen in the dried food was determined by the macro-
Kjeldahl method). Special attention was paid to the collection of urine, as the
major quantity of endogenic nitrogen is excreted fr^-R the body in the urine.
The urine was subjected to daily, clinical analysis, and the diure:.s, specific
weight, color transparency, and qualitative reaction for nitrogen were determined.
When nitrogen was present, the amount was determined. For the collection of urine
the dogs were harnessed with a special urine collector which consisted of a rubber
tube and bottle. Specially adapted cubicles were constructed for the dogs with
tile-covered floors with an opening through which the urine spas drained into an
al?~inum pan. The nitrogen in the urine was determined by the macro-Kjeldahl
method and then from this the daily amount of -,rotein was calculated.
~? To judge the balance accurately, it was necessary to compute the amount of
nitrogen excreted in the feces over the test period. The feces-were dried in a
porcelain dish over a water bath, (Found up in a mortar, sifted, and the nitrogen
determined by 'the macro-Kjeldahl method. We attached considerable importance to
the nitrogen in the feces, since the quantity may vary For different in2ividvals
and under different conditions.
The investigation was made on 35 dogs in six series of testa, the character
of the diet differing in various stages of the test. With;n each series, there
were instituted variations in the extent of protein and general starvation, size
of the doses of serum introduced, : amber of injections, etc., and the sia:.h series
was used as a control. To show the characteristics of the course of the test, we
cite detailed data on three of them, one of the most interesting from each of the
first three series.
The data arranged in Table 1 were taken from .the first aeries. Over the
course of 8 days, after a preliminary 6-clay period of protein starvation, 6.1. g
of protein in 100 cc of serum were introduced into this dog. Starting from the
first day of the introduction of serum, an3 subsequently to the compensation of
protein decomposition, the positive balance increased gradually; as a result of
which there was nearly 100 assimilation of the protein.
In the second series, the tests were conducted on animals?into which, during
the course of a few days the subject serum was introduced intravenously in place
of food protein, to establish the ability of the serum protein to replace meat
protein. Daily introductions of the subject serum were given to various animals
for periods of 6 to 25 says (Table 2). The data illustrate the relative utiliza-
tion of the protein of the intravenously introduced serum and. meat protein. The
table shows that the meat protein ingested in the period preceding the intro-
duction of serum is 40 to 50~ assimilated at that time, while the protein of the
serum, oa the average, is utilized better than 100, ~sic7, and up to 7096 of it
is stored in reserve.
In the third series of tests, we studied the effect of single and multiple
injections of the subject serum in different quantities of one-half, one, and
two portions of the nutritive protein over 6-25 days (Table 3).
Data of this series of teats are distinguished particularly in that in
addition to the growth of the positive balance, which continued at the same level
throughout the whole test; we noticed that the quantity of nitrogen excreted in
the feces was increased in relation to that of the preceding series of teats.
This shows that the protein of the sermon successfully fu].filis the needs of the
body.
Conclusions
1. The protein of species nonspecific serum, prepared from the blood of
cattle, is a valuable source of protein nutrition, and has an extremely high
biological value when introduced into the body by any method, particularly the
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intravenous, when it is almost completely assimilated.
2. The protein.oY the subject serum normalizes the.nitrogea metabolism sad
reduces to a minimum the catabolism oY nitrogen in the period following the intro-
duction oY the serum.
3. The excess protein oY the sub3ect serum is not excreted Prom the body,
as Yood protein is, but it is deposited in the cell tissues and expended as needed.
Therefore, the aub3ect serum is an irreplaceable preparation Yor pareatrxal intro-
duction in all disturbances oY nitrogen metabolism where there .is an urgent neces-
aity for creating an effective reserve to make up a protein deficit.
Lppended tables Yollow_]
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Table 1. Multiple Intravenous Introduction of Species Nonspecific Blood Serum
after a Proteinless Diet (Dog No 4 "Levlysyy")
Period'
Days
Wt (kg)
Full-Value
1
13
diet (vitro-
2
13
gea equil,ib-
3
13
rium)
4
13
Proteinless
1
13
-diet (period
2
12
preceding
3
12
introduction
4
12
of serum)
6
12
Introduction
1
12
of serum
2
13
13
~
13
5
13
6
13
7
13
8
13
Proteinless
1
13
diet (period
2
12
Polloving
3
12
introduction
4
12
Hof serum)
5
12
Balance of Protein
g during -day
Total
Per Kg
of Wt?
+0.9
+0.07
+0.4
+0.03
X1.0
+0.08
io.7
to.o5
_11.1
-0.85
- 7.9
-0.65
_ 7.4
-0.61
- 6.8
-0.56
- 6.5
-0.54
- 6.6
-0.55
- 1.7
-0.14
+ 0.3
+0.02
+ 2.3
to.17
+ 3.0
+0.24
+ 3.2
+0.25
+ 4.4
+0.34
+ 5.6
?-0.43
+ 6.0
+0.46
Excreted Protein
Introduced
g~during day
protein Dur-
Da
i
In Urine
In Feces
Total
y
ng
16.0
13.8
1.3
15.1
16.0
13.6
2.0
15?E
16.0
14.1
0.9
15.0
16.0
13.9
1.4
15.3
0
9.2
1.9
11.1
O
6.8
1.1
7.9
0
6.6
0.8
7.4
0
5.4
1.4
6.8
0
5.8
0.7
6.5
0
5.7
0.9
6.6
6.1
5.4
2.4
7.8
6.1
3.6
2.2
5.8
6.1
2.6
1.2
3.8
6.1
2.1
1.0
3.1
6.1
2.0 ?
0.9
2.9
6.1
1.1
0.6
1.7
6.1
0.1
0.l
0.5
6.1
0.1
0
0.1
0
3.1
1.1
4.2
_
1.9
5.8
0
5.0
1.7
6.7
0
4.4
0.8
5.2
0
3.3
l.5
4.8
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Period preced-
ing full value
diet of meat
protein
Succeedi,.g
period of
full-value
diet
Table 2. MultilnateadrofeMeat ProtEinc(Dog :io 17eCBlagorodnyyijic Blood Serum
Introduced
Excreted Protein
g during day
protein Dur-
Urine
I
In Feces
Total
Days
Wt ( )
ing Day
n
1
to
lo.0
7.7
1.2
3
1
8.9
8.4
2
to
lo.0
7.1
.
1
1
9.0
0
lo
7.9
.
~
1
.
lo.0
7.4
1.9
9.3
1
to
lo.o
i
7
l
i
0
2.8
2
10
10.0
.
4
1
.
1.~
2.6
10
10.0
.
1
2
3
4
10
10.0
1.1
1.0
7
0
.
1.9:
5
to
lo.0
1.2
1 3
.
0.6
1.9
6
l0
lo.o
1
to
lo.0
7.6
1.3
1.5
8.9
9.4
2
l0
lo.0
8
5
2.1
io.6
3
4
10
10
10.0
10.0
.
8.2
2.5
10.7
Balance ~f Protein
g during da~_
Per Kg
Total o~ Wt
tl.l t0.11
t1.6 to.16
t1.0 t0.10
+0.7 +0.07
+ 7.0 +0.70
+7.2 to.72
t7.4 +0.74 d
t~r?9 +0.79
t8.1 +0.81
t8.1 +0.81
+1.1
+0.6
-o .6
-0.7
+0.11
+0.04
-0.06
-0.07
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Table 3. Multiple Intravenous Introduction oY Species HoaspeciPic Blood Serum,
With Full-Value Diet up to and at Time of Introduction (Dog No 9 'Ryzhik-Pushok')
Excreted Protein
Introduced
Protein Dur-
Da
in
In Urine
g during da~
In Feces
Total
Period
Dater
Wt
y
g
Period priced-
1
13
19.0
18.2
2.5
20.7
2
Pull-value
i
2
13
19.0
17.1
2.1
19.
ng
trition (ni-
3
13 '
19.0
18.2
1.6
19.8
4
nu
trogen equilib-
4
13
19.0
17.3
2.1
19.
rium)
duction oY
t
I
1
13
25.1
16.1
6.6
23-3
ro
n
serum in Place
2
13
25.1
15.3
4.9
20.2
oY meat protein
3
13
25.1
16.0
4.1
20.1
4
13
25.1
15.4
4.7
20.1
5
13
25.1
16.1
4.1
20.2
_.
.
6
13
25.1
15.1
4.9
20.0
,
7
13
25,1
15.8
4.2
20.0
g
lg
25.1
15.5
4.6
20.1
Period
S
cceedin
1
13
19.0
18.4
2.5
20.9
g
u
~oY YY~11-value
2
13
19.0
17?`
2.4
19.9
nutrition
3
.
13
19.0
17.0
2.8
19.8
8
8
4
13
19.0
16.3
2.5
1
.
Balance of Protein
g during day
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