ELECTROPHORESIS OF ANTIBIOTICS

• Document Type: CREST
• Collection: General CIA Records
• Document Number (FOIA) /ESDN (CREST): CIA-RDP80-00809A000600390598-6
• Release Decision: RIPPUB
• Original Classification: S
• Document Page Count: 2
• Document Creation Date: December 22, 2016
• Document Release Date: October 18, 2011
• Sequence Number: 598
• Publication Date: June 9, 1951
• Content Type: REPORT

CENTRAL INTELLIGENCE AGENCY
INFORMATION FROM
FOREIGN DOCUMENTS OR RADIO BROADCASTS
COUNTRY us3R
SUBJECT Scientific -Medical, antibiotics
HOW
PUBLISHED Semiweekly newspaper
`.NHERE
PUBLISHED Moscow
DATA
PUBLISHED 2e Apr 1951
LANGUAGE
DATE OF
INFORMATION 1951
DATE DIST. y .Tun 1951
SUPPLEMENT TO
REPORT NO.
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or rra ur?no aTAnla tlirla TNa raAlllra 0/ raMOEArI ACT w
r. a. c.. a? Aro u.Aa Anrna. rts nArannmr or r ~ aaraunw
or m corraan a Arr uawa ro ?r rrAUrron:ao raraw n -ro?
alalnla r7 LAr. aanoarrnaa or rM?7 roar It /rrrlanw.
Meditsinakiy Rabotnik, Vol XIV, Bo 28 (944), p iE.
The`method of introducing medicinal substances into the organism by
means of a direct current (electrophoresis) has been known for a long time.
The substances to be introduced into the organism accumulate in the skin,
and after penetrating from there into the lymphatic and blood streams, are
slowly eliminated from the organism. The skin receptors, in this process,
undergo a direct irritation, as a result of which complex reflex reactionr~
take place. These reactions ve-y depending oa the quality of the irritant
and the state of the organism.
The ideas of Z. P. Pavlov on the action of medicines as reflexogenic
irritants played an important role in solution of problems on the effect of
electrophoresis of medicinal substances.
The experimental department of the Central Scientific Research Institute
for Physical Therapy Methods a~~ Yslta (director, Docent 0. V. Grebova) worked
out the problem of electrophoresis of penicillin..
Thin work is being carried out by ua together with associates of the
experimental and clinical departments of the .institute, 3. I. Volkhonekiy,
A. I. Sorkin, 0. G. Mats?ko, and others. The investigations showed that
antibiotics carry an electric charge ahd can migrate under the effect of a
direct current, penicillin towards the anode, and streptomycin towards the
cathode.'
In the electrophoresis of penicillin from the negative pole, and in elec-
trophoreais of streptomycin from the positive pole, the antibiotics are de-
tected is the blood and in the urine. In the usual method of else+.rophoresis,
the accumulation of H and OH ions in solutions of antibiotics lowers their
activity. For that reason, especially designed non-polarizing electrodes were
used.
CLASSIFICATION. `_T_
Qfc OE~~CIAL USE_09~L~1
Sanitized Copy Approved for Release 2011/10/19 :CIA-RDP80-00809A000600390598-6
 Sanitized Copy Approved for Release 2011/10/19: CIA-RDP80-00809A000600390598-6
However, since these electrodes are not yet in vide use, a simplifie4
method for the electrophoresis of penicillin can be recommended.
Fitter paper or two to three layers of gauze with a surface of 150 to
250 sq cm are moistened with a penicillin solution containing 10,000 to
20,000 international unite per 8 to 10 ml distilled eater, and applied :o
the akin, previously gashed and moistened with distilled eater. A hydro-
philic fabric pea of the same size and approximately 1.5 to 2 cm thickness
is placed on top of this surface. A semipermeable membrane made of animal
matter, moistened with glycocoll, is placed between the layers of the pad,
closer to the outer surface. This membrane serves as a buffer s?.ibatance
which binds electrolysis products. A metal plate, connected by a wire to
the cathode of the galvanic plate, is placed on the outer surface of the
hydrophilic pad.. The second electrode, the anode, conforms to the customary
type, but with a lining of the same thickness as that on the cathode. The
current is strong, 20-3J ma. The procedure is carried out once daily, and
lasts 20 to 30 min. The course of treatment consists of ten to 20 such ap-
plications=
The above method can also be used for the electrophoresis of streptomy-
cin, which is introduced from the anode. For this purpose a calcium chlo-
ride preparation of streptomycin moat be used.
The electrophoresis of penicillin does not produce any ill effects what-
soever in the patient. Only s more intense and protracted reddening of the
skin than occurs in ordinary electrolysis is observed. In about 5096 of the
patients, an increased skin reaction was observed after five to seven treat-
ment~ and within 2 to 3 days, inflammations appeared at the area of the.lo-
cation of the cathode. The dermatites are distinguished by small or large
nodes. To prevent dermatites, it is necessary to change the location of the
electrodes and the current density.
Experimental and clinical observations indicated the possibility of in-
troducing penicillin into the tissue?of the middle ear by means oS a gal-
vanic current applied through the external ear passage. It was also estab-
lished-that electrophoresis of penicillin in many cases contributes to the
reduction of inflammations appearing in cases of infectious radiculitis and
neuritis, infectious 'meningomyelitis, suppurative blepharitis, and con~unc-
tivitis .
The favorable effect of electrophoreaip of penicillin was noted on a
cumber of patients whose treatment by other medical and physical means had
not proved sufFiciently effective. The effectiveness of this method was '
studied at the polyclinic department of the institute in the treatment of
furunculosis, hydroadenitie, Morvan?a disease, and other surgical diseases.
Upon combination of electrophoresis of penicillin with climatic and
physiotherapeutic methods, such as air and sus therapy, UF.F, sea bathing,
and others, improvement is achieved quicker than in cases where penicilliA
electrophoresis is used alone.
The experimental observations carried out by ua together with the as-
sociates from the Yalta Institute of Tuberculosis Climatotherapy showed that
in intraperitoneal infection of guinea pigs with tuberculosis bacteria, the
life span of the animals treated with etreptomycia electrophoresis was longer
than that of the control animals.
These observations served as the basis for the further investigation of
the effectiveness of streptomycin electrophoresis for the~cure of tuberculosis
in several sanatoria on the southern shore of .the Crimea.
Sanitized Copy Approved for Release 2011/10/19: CIA-RDP80-00809A000600390598-6