NEW USSR LIVER PREPARATION ANTIANEMIN

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Collection: 
Document Number (FOIA) /ESDN (CREST): 
CIA-RDP80-00809A000700230136-4
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RIPPUB
Original Classification: 
C
Document Page Count: 
3
Document Creation Date: 
December 22, 2016
Document Release Date: 
July 13, 2011
Sequence Number: 
136
Case Number: 
Publication Date: 
May 13, 1955
Content Type: 
REPORT
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Sanitized Copy Approved for Release 2011/07/14: CIA-RDP80-00809A000700230136-4 CLASSIFICATION CENTRAL INTELLIGENCE AGENCY REPORT INFORMATION FROM FOREIGN DOCUMENTS OR RADIO BROADCASTS CD NO. COUNTRY ussR DATE OF SUBJECT Scientific - Medici INFORMATION me , emopoiesis, radiation sickness DATE DIST. /3 May 1955 NO. OF PAGES 3 SUPPLEMENT TO REPORT NO. THIS IS UNEVALUATED INFORMATION PUBLISHED Irregular periodical WHERE PUBLISHED 1:oscow DATE PUBLISHED 1953 LANGUAGE Russian ..,? ....... T . ., ........ .... ... ....~~........ --- --------- - Sovremennyye Probleu~y Gematologii i Perelivaniya Krovi, No 3n, Yt ~: G (4 113,1 USSR LIVER PREPARATION ANrIANFbIIN Kh. Kh. Vlados (deceased), E. I. Terent'yeva, M. G. Kakhatelidze, T. A. Cherntsova [Comment: In addition to the applications mentioned below, the new Soviet liver preparation antianemin has been recommended for use in the treatment of disturbances of the blood formation mechanism that arise in connection with radiation sickness (cf. +. V. Kozlova, "Radiation Sickness," Vestnik Rentgenolo ii i RudioloGii, No 4, 1954, pp 38-43). In -view of the emphasis placed on the activity of cobalt in the discussion below, one may con- clude that various drugs containing cobalt, in combination with liver preparations or without them, will presumably be investi- gated in the USSR, or are being investigated there at present with the view of using them for the treatment of disturbances of hemopoiesis. The following report is based on a contribution from the Central Institute of Hematolo, and Blood Transfusion.] The liver preparation antianemin, which is being proposed by us for the treatment of anemia, has been prepared by the Central Scientific Research Laboratory of Organotherapeutic Preparations ["Organopreparaty"] attached to the Meat Combine[not further identified] and tested by us from the stand- point of its hemopoietic activity. To establish whether or not preparations of this type are therapeutically effective, it is necessary to demonstrate that they have hemopoietic activity. Hitherto no method has been available which made possible a reliable qualitative and quantitative determination of the activity of liver preparations. Singers test, a reticulocytic reaction used for the determination of hemopoietic substances in gastric juice, does not yield any quantitative data. Furthermore, the Singer test is not precise, because reticulocytosis cannot serve as a reliable indication by reason of its instability in the experimental animals used (rats). STATE ARMY CLASSIFICATION Sanitized Copy Approved for Release 2011/07/14: CIA-RDP80-00809A000700230136-4 Sanitized Copy Approved for Release 2011/07/14: CIA-RDP80-00809A000700230136-4 The Cytological Laboratory [of the Central Scientific Research Institute of Hematology and Blood Transfusion] has developed a method of hemocultures which enables us to establish qualitative and quantitative differences in the activity of hemopoietic substances contained in various liver preparations. With the aid of this method of tissue culture, more than 200 liver preparations obtained by procedures which differed in various details were tested under laboratory conditions. The results of the laboratory investigations showed that the prepara- tion which has been named by us antianemin is more active than campolon or the standard grade c: liver extract. The advantages of antianemin in comparison with the available liver prepa- rations are the simplicity of the method of preparation and its relatively low cost. The liver preparation antianemin, into the composition of which we intro- duced cobalt, has baen prepared by the Central Scientific Research Laboratory of Organotherapeutic Preparations in forms suitable for intramuscular adminis- tration and for peroral use. After antianemin had been tested for effective- ness and subjected to various biological tests, such as checks for sterility, tests for absence of toxicity, and determination of the effect on blood pres- sure, it was referred to the Hematological Clinic of the Institute [Central Institute of Hematology and Blood Transfusion]. Investigations dealing with the effect of cobalt on hemopoiesis were originally published in 1929. After being administered in small doses, co- balt exerts a stimulating effect on the bone marrow and for that reason con- tributes to producing a rise in the level of hemoglobin, erythrocytes, and reticulocytes in the peripheral blood. In experiments carried out on rate, it was established that cobalt produces polyglobulism. When cobalt is intro- duced into the organism, no reduction of the oxidative capacity of the blood takes place and no effect on the formation of methemoglobin in the blood is observed. Cobalt is easily eliminated from the body. According to the data of Berzin and Koval'skiy, a dose of cobalt amounting to one gram per 100 kilo- grams of weight of the body of a calf does not produce any deleterious effect. Cobalt is widely used in the therapy of some nervous and infectious diseases and also in the treatment of anemias of various origin. Analysis of the ash of crystalline vitamin B12 that has been isolated from liver indicates that this ash, in addition to phosphorus, contains a large amount of cobalt, i.e., close to 4 percent. Vitamin B12, in addition to exert- ing an active effect on erythropoiesis in pernicious anemia and other forms of anemia, is also a growth factor. At the hematological clinic, antianemin containing cobalt was administered to 38 patients. Twenty-seven of these patients received the drug parenterally. Nineteen of the patients treated had the Addison-Biermer disease, while eight had other forms of anemia (sprue, chloranemia, anemia following resection of the stomach, hemolytic anemia, and macrocytic anemia). In treating the 19 patients who had the Addison-Biermer disease, 2-4 grams of antianemin were administered per day. The total quantity administered during the course of treatment amounted to 44-1C0 grams. An increase in the quantity of hemoglobin amounting from 21-44 percent to 61-77 percent and comprising one percent per day was observed, while the number of erythrocytes increased from 930,000 to 3,500,ooo-4i370,000. There was also an increase in the number of leukocytes and thrombocytea. Immature forms of granulocytes disappeared, and the number of lymphocytes approached normal. No recurrence of the disease was observed in 16 patients, while three patients had recurrences within 7, 12, and 14 months, respectively., after completion of the course of treatment. Sanitized Copy Approved for Release 2011/07/14: CIA-RDP80-00809A000700230136-4 Sanitized Copy Approved for Release 2011/07/14: CIA-RDP80-00809A000700230136-4 C-O-ITT-F-I-D-E-N-T-I-A-y During treatment with antianemin, toxic phenomena or infiltrations at the sites of ,injectipn were not observed. When E00-1,875 grams of antianemin were administered perorally during 13-47 days for the treatment of the forms of anemia mentioned above, an increase in the quantity of hemoglobin and in the number of erythrocytes was observed. The average daily increase by one percent of the quantity of hemoglobin, which has been achieved by administering antianemin containing cobalt, is higher than that attained with the use of other liver preparations. Antianemin must be assumed to be a more effective preparation than campolon, because its application results in an earlier abatement of the symptoms of anemia. C-O-N-F-I-D-E-N-T-I-A-L Sanitized Copy Approved for Release 2011/07/14: CIA-RDP80-00809A000700230136-4