THE WORKS OF THE INSTITUTE OF INFRAMICROBIOLOGY OF THE R.P.R. ACADEMY

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December 7, 1962
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Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Next 1 Page(s) In Document Denied Iq Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 THE WORKS OF THE INSTITUTE OF INFRAMICROBIOLOGY OF THE R.P.R. ACADEMY (ABSTRACTS) 1949-1961 F ~ ICI ~ Ufl ONLY GPOUP 1 STAT Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 THE WORKS OF THE INSTITUTE OF INFRAMICROBIOIDGY OF THE R0 P. R. ACADEMY Abstracts ) 1949 1961 FOR OPEC USE ON 11Y Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 CONTENT S li Introduction I.- General inframicrobiology 1 56 e I o 1 II,- Viruses and tumors 57 - 86 0 0 31 IIIe- Dermato-viral diseases 87 94 s o 47 IYm- Viral hepatitis 95 - -18o ? 52 V e4? Enteroviral diseases 181 -223 .1o8 VI om Neuroviral diseases 224 -339 0136 VII Respiratory viral diseases 340 ?50o e205 V IIIe-Animal viral diseases 501 -587 .299 IXomRickettsial and pararickettsial infections 588 .643 .365 Xe= Varia 644 -722 ? e395 XI"I- Monographs 1 - 18 e 9439 Alphabetic index FOR OFFICIAL 451 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 INTRODUCTION The abstracts of the investigations of the Insti- tute of Inframiorobiology of the R.P.R.Aeademy in its 13 years activity, is both a summation of the persevering work, carried out by the research workers of our Institute and a hommage brought to those who have helped to obtain these results. They also reflect the wish that the works of the Institute should, be known by specialists at home and abroad, by all those who are interested in our specialiya However, in the first place, we should like.to express by this publication, our sincerest thanks to the Rumanian Workers' Party and Government, that fully under- stood the importance of inframicrobiology, already 13 years ago, and created and endowed the Institute we are nowwork- ing in; that have moreover decided to develop and build a new institute, which will be very soon ready; that have given'us immense possibilities for fruitful researches, that have brightened our work and life as investigators in the unknown world of virology - a thrilling work. in itself-, th4t have honoured us by entrusting us with new tasks, when the first medical problem was stated in the directives of the IIIrd Congress of the Rumanian Workers' Party : the problem of viral diseases. Our institute, the only large unit for the study of virology in the country, has assumed the task o an authority in the methodology of our speciality, of a center for graduate and specialization teaching and, in answer to the=various appeals of the tutelary bodies, of the obli- gation to investigate. the most varied problems. It is for this reason that the number of problems and themes studied 0" in, - M fUR Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 II iii relatively great. In the 13 years of its existence, in the Insti- tute of Inframicrobiology staffs of specialists have been trained, instruction has been given and research work has been carried out; research destined to bring its contri- bution to science in the country and abroad. Science has been linked to practice by the help given in the field in the control of viral diseases and for the improvement of public health; useful ideas on virology have been broadcast by lectures, article, papers and booklets. Fundamental ,sca.entific research, this reserve of future technique as it is.called by Lenin, is our principal aim, of all those working in the institute. Research however has never been separated from practical applications. It is true that many works have been pub- lished concerning results obtained in problems initiated by as (ultrafiltration, megamononucleosis, synchronophy- laxix, septineuritis, hydropigenous neurovirosis, tissular immunity, paraimmunity, the biophitic state, viral-onco- lysis, metallized viral vaccines, the plurality and varia- bility of the hepatitis virus, etc.), or in problems that have long been studied (cellular inclusions, inframicrobial histopatholo?y, vaccinia, rabies, poliomyelitis, herpes, inframicrobial variability, etc.), or, more recently , (the viral etiology of cancer, Coxsackie, psittacosis, A..P.C., ECHO, interferons, in vitro cell cultures, electro- phoresis, chromatography electron microscopy, labelled atoms, Pavlovism in virology, cytochemistry, biochemistry, viral biosynthesis, ionizing radiations, etc.), but it is none the less true that in view of the actual requirements of medicine in our country, we have been constantly preoccu! pied by the applications of science as demanded by our go- verning bodies. We shall recall the vaccines studied against 14 human or animal viral diseases : small pox, swine plague, avian plague, fox encephalitis, avian diphteria-variola, 0 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 III hydropigenous neurovirosis, influenza, ,psittacosis, rabies,- mumps, Aujeszky's disease, lymphocytic meningitis, foot and mouth disease, tick-borne encephalitis; another two vaccines are under study: APC and anti-measles vaccines. Some of these are already widely applied in the population or for the immunization of animals and birds. Numerous diagnostic tests for viral diseases, some of which original, may likewise be mentioned. Instruction given in the field in. various centers, to`a great number of doctors, in view of the application of these tests, has likewise been one of our objects. Hundreds of work-days have been spent, at the demand of the.Ministry of Health and Public, Welfare, in order to come in aid to epidemiolog- ists. Two of our units, at the Colentina Hospital and Hos- pital of Contagious diseases no.2,-perform diagnostic tests and virus isolations from patients The isolation and identification of viruses in clinics or.,.in field work have made...evident for .the first time in our country the etiologic agents of certain human or animal viral diseases : ECHO, APC, tick-borne encephalitis virus, pneumonia, fox encephalitis,;:-epizootic abortion, pneu- monia of calves, ornithosis, recurrent meningitis viruses and the agents of various human and''animal neor.ickettsial affections, etc. The':scientific works of the investigators of the Institute,numbering about boo, deal with general and spe- cial virology, in which 24 human viral diseases and 13 ani- mal and insect viral diseases are studied: herpes, vaccinia, rabies, sueta miliaris, epidemic hepatitis., lymphocytic meningitis, measles, German measles, poliomyelitis, influenza, encephalitis, atypical pneumonia, parapoliomyelitis MM, Coxsackie, mumps, infectious mononucleosis, infantile in- fectious lymphocytosis, neorickettsial pneumonia, recurrent viral meningitis, ornithosis, tick-borne encephalitis, adenoviral and ECHO infections, malignant lymphogr.anuloma- tosis , moluscus contagiosum; fowl plague, swine plague, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Iv fowl pox, distemper, Newcao.,., disease, clavella, Aujesky's disease, fox encephalitis, canine hepatitis, swine enzootic pneumonia, anemia of horses, hydropigenous neuroviroses of fish, jaundice of silk worms. In epidemic hepatitides, or1'e of the most impor- tant contributions is the fact the plurality of viral hepa- titides has been established by the description of new forms, sclerogenous hepatitis and subsequently a hepatitis due to virus NHA. In the course of the investigations carried out, inclusions characteristic of Botkin's disease were discover- ed, as well as the topography of the virus in the hepatic cell (St.Nicolau, R.Portocald at al.); the electronooptical morphology of the sclerogenous virus was established (St.Ni-' colau at al.), its hemoagglutinatitg properties (N.Cajal at al.), serologic differences between the sclerogenous and Botkin's virus (St.Nicolau at al.), the distribution of the sclerogenous virus in the human organism (N.Cajal at al.), the duration of the elimination of hepatitis viruses from the organism and the presence of virus carriers (N.Cajal et a1.); the "zone" phenomenon has been described and the non- specific factors that interfere in the hemagglutination and hemagglutino-inhibition reaction; it was possible to isolate and maintain in serial passages the sclerogenous and. Botkin's virus in embryonated eggs (St.Nicolau at al.); detailed morpho- logic studies were carried out establishing the general cha- racter of the viral disease (St.Nicolau at al.); different biochemical aspects of the affection were studied (R.Porto- ealA and V.Boeru); serum aldolase was determined and its diagnostic value established even in the unapparent forms (N.Cajal at al., D.Mitroiu at al.); a comparative critical study of the various diagnostic tests were performed (N.Ca- jal at al.); hepatic and muscular aldolase was determined (P.Athanasiu and N.Cajal); the diagnostic value of the A.V.B. test and agglutination of Rhesus and chick red blood cells in this viral disease (G.Balmu$ and D.Mitroiu); interesting epidemiologic studies were also carried out (St.Nicolau, G.Marinescu, N.Constantinescu, N.Cajal, etc.) - Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 V Spontaneous variability of hepatitis viruses under Aatural conditions was demonstrated and the k,!urality of the epidemic hepatitis was interpreted in the light of this variability (St.Nicolau'and N.Cajal); certain strains, that appear to have etiologic relationships with this viral disease, were isolated in cell cultures (D.Mitroiu and co- workers); a study was carried out on the relationship between canine and human hepatitis (Surdan j j,: - 1ti, ) , and murine hepatitis viruses (N.Cajal e.t ,.io) etc, lost of the results of lumanian investigations in the fiel& of epidemic hepatitis are summed up in two monographs publishes by the R..e.x. Academy. Numerous works have been published in the periodicals at nome and abroad. For the resu.its ob,ained in -The field of epidemic viral nepaUtiis, the i Class State Prize of the R.Y.R. was awared in 1953; aftd for special biochemical studies in this connection, R.Portocala received in 1957 the "V.Baber?" Prize of the R.r.R.Academy. Recently, at the International Congress of infec- tious Pathology in Milano, we had the satisfaction to'find that our conception on epidemic hepatitis, concerning the plurality of the virus and its variability in natural condi- tions, have been accepted by all the specialists in this field. in influenza and other viral diseases of the res piratory tract, various influenza virus strains -- isolated- _. in the country since 1953 and up to date were investigated (A. De,i sib aW,);tne dynamics of hemagglutination-inhibition ax~'!bodies were studied in the population, thus confirming the data regarding the variability in nature of this virus (A. Der Otil it'a3..) An ezticient ovovaccine was prepared in a close-circuit apparatus, specially deviced in the Institute and successfully applied in the field (A:Derevici); a diagnostic test based upon the precipi anion phenomenon was developed (A.Derevici and N.DragAnescu). A study was carried out on cytologic diagnostic methods of the Koleaditzkaia or Yanthier.type (Al.BroniVk.i) Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 VI and a. critical experimenta_ ?y on diagr.. o s,ic tests (tim8araVearbu and N.Draganescu). J pidemics due to viruT A (Asia) were described (A.Derevici et al.); for the first time in the country, influenza virus type D was isoya-.e (D. rarchila et al.). Modification of the influenza virus was obtained by vegetative cross-cultures with otner viruses., especially neurotropic viruses (P.Stroescu, Ai.retrescu, cord,ical dynamics in the course or ani.i-influenza vaccina- tions in man anti animal and circulatory and respiratory changes in the course of that disease was studied (L.MI rza et al.). The .role o1 i-Ai influenza virus in the etivpathu- geny or the corticomseptic syndrome in infants was demonstra? yed (N.Cajai et al.); the action of embryonic fluids on 6he influenza virus was established (N.Cajal and M.Cep- leanu); investigations were carried out on the experimental disease in animals with gonadic hypoactivity (C.Baba and N.Cajal); investigations were done on influenza virus cultures in the de embryonated egg (m. uepleanu, Y. c.opelo- vici); anti-influenza immunity in animals previously in- fected with the 'vac'cita virus (DaSI.ra'eanu and B.Fuhrer); influenza morpnopathology in the suckling mouse (G.Mari- nescu et al.); the virus in non-epidemic periods and iso- lation of 'a virus with neurotropic properties (Ai.BroniVki et al.); interference between various influenza viruses (G.Danieiescu); evolution of the experimental disease in :Largactyi treated mice '(N.Cajal et al.). From cases of swine influenza, influenza virus type A was isolated, demonstrating the role of these animals in the spread and maintenance of the influenza infections (N.Cajal and M.Taga); serum antibodies were studied by microelectropnoresis (R.Portocala and V.Boeru); interesting research work was carried out on replication of influenza virus, obtaining biosynthesis of the viral elementary bodies following introduction in the embryonated egg of purified ribonucleic acid extracted from the influenza virus (R.Portocala et al.); the electronic aspect.oi' the virus thus obtained was desc.cibea Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 VII (R.Porto,~.ala et al.); the action of ribonuclease on influenza lirus mui'Uplication (Y.Copelovici and 1".Cep)eanu); the p1ysical mixture or two virus types was described, thus -explaining the mixt virus (R.Portocala); the action of vitamins C, PP and B was studied (G.Popescu); interference and the mechanism of action of interferon (G.Danielescu); the allergic factor in influenza (A.Derevici et al.). The neutralizing action agains-u -tae influenza virus of bee royal jelly was discovered (A.Derevici et al.); dia.gnastic sera and anti?eus were prepared'(A.Derevici); new hype ox virus that produces a disease similar to influenza, the Boto virus, was isolated (A..Petrescu et al.); the action of ionizing radiations on influenza and Hi an-6lboaieS was in- .-estigated (O.Burducea and M.Cepieanu), etc. For the first time in the country, APC viruses were isolated and investigations were carried cut on Lhe alterations produced by Leese viruses in human embryo cells (I.Aderca and M.Ianconescu) and on anti-APC antibodies in the population (A.Derevici'-and co-workers;-M.Gruia and co- workers). The infectious role of DNA extracted from APC virus was established (R.Portocala et al.); the sensitiviuy of different cell types t.o Leese viruses was studied (L. Preaescu and co-workers). It is likewise for the first time that germs of the Gi- I,M~A neorickettsial group were isolated in the country and s-,uaiea in the electron microscopes (D,Si?lueti,al.) , these germs T,=re trasmitted to the cotton rat (N.Drag:nescu, D.Sara ea- rIL); ocular lesions produced by neorickettsias (G:Marinescu) were described for the first time in the country, psittacosis pneumonias were detected (~.Sto ) and numerous ornithosis strains were isolated from man and different animal species following epidemiologic and epizootologic surveys (D.Sar#eanu C. ;urdan et.. al.) ; a metallized vaccine was prepared, as well as antigens and diagnostic sera (D.53rateanuet.,al.);the etiologic correlation between these germs and various cases of cardiovascular diseases was studied (St.Nicolau et al.),etc. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 . VIII As regards the --Le of rickettsias and pararickett? Sias L, the e- :L,j.,wj ;fir of cej.? . n .tauma:a di5uases, our invest-' gato:cs ioye, -shown tie role or- these germs in the etiology of certain cardiovascular affections (infarction, arbe.L1tis, migratory pniebitis, etc.) ~St'.Nicoiau, C.Surdan et al-) q gastric ulcer-(8.Nicolau, Ai.re-trescu et al.), infantile .pneumoencephalitis (N.1ara ;arHscu et al.) , certain chronic respiratory affec,,6ons - (puimonary sclero-emphysema", chronic bronchitis, bronchiectasis, etc.), the pathology of preg- nancy, mortinatality and conge malformations (N.Cajal, C.Surdan et al.) ; stet' - :_c investigations with antibiotics or the tety? Q*c~e:9 group have yielaea important data, which on the one hand confirm the rickettsial or pararickettsiai ebiolugy of these affections-~ and on.the ocher, open wide prospeebs in the prevention and therapy 'of these aisee&ses, that consc?itute an importanb problem of public heal.bhe Rumanian invebbigation on influenza were synbhebiz- ed by A.Dei-evici in*her monograph "G?ipa" aspecte din pr,ob- lema (influenza --aspects of the problem)., publishes. by- tne R.?PBt.Ac ademy, those concerning ornithosis will appear in a mono g,-apn written. by 1):.SArAVeanu,. ana those on adeno- viruses in amonograph by -A?Deievici, in the press. Field activity and'investigations during the in- fluenza epidemic of 1958 have been summed up in the volume ""Gripa,.date asupra epidemiei ain 1959 ( Influenza m data on the 1959 epidemic), published by zditura Medical&. In rabies,_ the. comparative action of various antirabies vaccines was studied (bLL Constantinescu ?~;,al.)ga vaccine was prepared with a fixed methylic alcohol attenuat- ed virus, suspended in mineral oil (S?.Nicolau.ot.,..-.p);'?closed rabies" and the important role of this notion in the pro- phylaxis of rabies were described (S.Nicolau and N.Constanti- nescu);. the priority of V.Babes in the discovery of rabies inclusions o the Babes-Negri bodies o was established (Sts Nicolau and M.Babes). Thus, inclusions have.been describes in the retina of naturally or experimentyinfected animals (x..Fortocala and N.Cajal), as well as in the carotid glomus Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 IX (P.Athanasiu et al.); histopathoiogic studies of the nervous vegetative system in this viral disease have likewise been carried out (P.Athanasiu et al.); a rapid method of diag- nosis by the phase contrast microscopy was aeveloped (N. Cajal and S.Mateescu); cases or rabies with a long incubation were studied (S.Nicolau.et al.); the part played by medicinal and reflex sleep in the evolution of this viral disease (S.Ricolau et al.); the' early onset of rabies detected by means of conditioned reflexes (L.Mirza et al.); the unapparent disease in the course of antirabies vaccination detected by means of the method of conditioned reflexes (G.Danielescu et al,); the role of cortical reactivity in the-course of anti-rabies immunization (1. Cajal and G. Popescu); the important part played by association of immune serum in the prophylaxis of rabies (N.M.Constanti- nescu et al.); inclusiogenesis in animalb subjected to the action of ionizing radiations (S.Mateescu); "the law of intervals" applied 'o anti-rabies immunization (N.Constan- tinescu et al.); the comparative immunogenic value of the Flury strain and the glycerophenol treated vaccine was studied (L.Paeeu',et balo) : _. a silvered active vaccine was prepared (St. NicolaaetaL) and" dissemination in the organism of viral elementary bodies labelled with radioactive silver was followed up (Q.Nicolau e0); a new ft agnostic test - the peritoneal test - was devised (S.Nicolau, N.Constantinescus) and the following studies were-carried out : the action of cortison, X-rays and isotopes on experimental rabies and antirabies immunity (N.Cajal), rabies in the hamster, the sensitivity of.this animal in experiments with rabies virus (N.Constantinescu. et. i7 )eq;0Recently, St.Nicoiau, N. Constantinescu and N. Cajal have published their monograph on Turbarea (Rabies) (The Publishing House of the R.P.R. Academy). In poliomyelitis and related diseases, a detailed histopathologic study of the sensitive nervous system was car- ried out (St?TicoliLu 6t:a1); a histologic study on experimental Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 poliomyelitis with the Lans;.ng virus (El.Lissievici Oprescu); the poliovirus wart cultivate: in HeLa, Detroit 6 and human amnios cells and comparatively in human embryo cells (I0Adeb_ .ca et,,al.);poliomyelitic myocarditis, hepatitis, nephritis and pneumonia were described (G.Marinescu et al.); t:e incidence of anti-Lansing antibodies was established in the population of Bucharest, and the origin and significance of these antibodies discussed (N?Cajal et al.). The idea of paraimmunity in herpes-poliomyelitis'was developed (Sts Naoo)lau and M.Ianconescu); a study was carried out in cell ciz~,Lures on antibodies to the three poliovirus types, in the inhabit.en'ts of various towns and villages in the couri= t.Ly et al.) ; the e voiutiun of antibodies in children vaccinated with Salk vaccine was followed up (,N.Cajal et al,); electrophoretic modifications in vaccinates persons was studied (G.Marinescu et al.); the lesions produced by polio- virus in embryo and human amniotic cell cultures (i.Aderca and M.ianconescu); for the first time the poiio vaccine prepared in the Puiiomyelitis ins'r itUue of Moscow wizh modif.Led Sabin strains was appiied in tyre country and eli- mination of he virus and serum tests were studied (N.Cajal et al.); the action of radioactive isotopes on type seru- neutralization antibodies (O.Burducea); the poliovirus neu- tralization test was described (St.Nic?iau et al.), etc. Numerous Coxsackie virus strains were isolated and the etio- logic relationship with poliovirus studied; the aspect of the myofibrils of mice infected with the Coxsackie virus were investigated in the electron microscope (R.forzocala et al.); muscles extracts from Coxsackie infected animals (Rorortocala et al.); Coxsackie A. virus was transmitted to beta and gamma X-ray irradiated adult mice (N?Cajal and The rirsz EeHu virus strains were isolated in nnn-t;r;y (M.Gruia et al.; i?Aderca et al.) and investi- ; n prri.ed out on anti ECHu antibodies in children's con . -Yn e in Buchare t, (M.Gruia, Y.Copelvvici) and HAS anti r)-.d:r es (M0Grua.a et al.; G.Marinescu). ECHO virus strains Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 XI were isolated from various cases of .fant' :`.e enteropathies (M.Gruia at al.), etce Acad. St.S.Nicolau, acad.Al.Radulescu, N,Cons- tantinescu, N.Cajal and G.Marinescu have drawn up a documented monograph containing foreign and Rumanian works in the field of poliomyelitis. In herpes it was possible to transform the relaps- ing herpes virus into herpes simplex virus (St.Nicolau at al.); the characteristic lesions produced by herpes virus in the retina was studied (R.Portocala and N.Cajal); investigations were carried out on-the experimental disease in-animals with a modified reactivity of the cerebral cortex (N.Cajal at al.); the influence of vitamin C on experimental herpes in the rab- bit.(N.Cajal and C.Baba); the evolution of herpes in animals with aloxan-induced diabetes (C.Balmuq ar,' N.Cajal); herpetic "diabetes" was described in rabbits (R.Portocala and N.Cajal); the relationship between herpes and poliovirus (St.Nicolau et al.); the evolution of experimental herpes under the action--of cortisone (N.Cajal and G.Popescu), and gonadic hypo- and hyperactivity (N.Cajal and C.Baba); antiherpetic immunity in irradiated animals (S.Mateescu); virus isolation in cell cultures (M.Ianconescu et al.); the incidence of antibodies in the population (M.Ianconescu); lesions produced by herpes virus in human embryo cells (I.Aderca at al.). In lymphocy-ticmeningitis, numerous virus strains were isolated (A.Derevici at al.) and an ample clinical, epidemiologic, and-experimental study of the disease and histiopathogenesis of the experimental disease was carried out (A.Derevici et al.). Comparative serologic studies were done, showing the best diluting fluid for experimentation with these viruses (N.Cajal and Y.Copelovici); studies were carried out on the etiology of meningitis cases hospitalized in Bucharest (M.Gruia et.al.) and on methods of etiologic diagnosis (N.Cajal). A virus producing recurrent meningitis was isolated (N.Draganescu) and a monograph published on lymphocytic meningitis (A.Derevici). Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 XII In encephalitj ,;,o, several vi uses were isolated and vast serologic and histopathologic studies carried-oat on various types of human encephalitis and especially tick- borne encephalitis (N.DragAnescu et al.). In connection with this disease, the incidence of complement fixing anti- I.-bodies in forest workers was followed up (D,SArAVeanu et al.) and a metallized vaccine developed (St.Nicolau at al.). Multiplication of the encephalommyooarditis MM virus in tis? sular cultures was likewise studied (N.Cajal et.al.); an effi- cient metallized vaccine-was prepared (St0Nicolau, N.Draganes? cu at al.) In mum, the viral elementary bodies were studied on smears obtained by puncture'of.the parotid glands and stained with phenic isamine(S.t-.Nicolau and co-workers); the non-specificity of the inclusions found in the salivary glands was demonstrated (St.Nicolau et al.); numerous mumps virus strains were isolate'd' 'in .embryonated eggs from the cerebrospinal fluid of patients with the parotid or meningeal forms; comparative investigations on the value of serologic reactions were done; the behaviour of the virus in the embryonated and 'deembryonated eggs (M.Cepleanu); a vaccine was prepared with autochtonous strains, applied in the field (YoCopelovici et al.) and the vaccines prepared according to various techniques were studied comparatively (Y.Copelo- vici and M.Gruia)? In Aujeszky's disease, epizootological and clinical investigations were carried'out (C.Surdan at al.); the spe- cific histopathology of the natural and experimental disease was studied in detail (St.Nicolau at al., C.Surdan at al.); adaptability of the virus to the chick embryo was investigated Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 XII I and attempts were made at preparing a vaccine with the c .itura virus (.Suhaci et al.); an active hyperimmune serum was prepared experimentaly (v.Tomescu et al.) in fox encephalomyelitis, epizootological, clinical, experimental and histopathologic studies were carried out and a vaccinescurrent-11.y applied, saved many breeders in fox 'breeding farms; the etiologic unity of fox encephalomye- litis and canine contagious disease was demonstrated and the relationship of this virus and human hepatitis viruses in- vestigated (C.Surdan et al.) In vaccina new method for the improvement of vacc3 in lymph production was developed and the notion of e- p1thelio-vaccinia and pyovaccinia were introduced (St..Nicolau at al,). Investigations were carried out, on the cation of serum gammaglobulins in the immunization process (R...Porto- cala and N.Cajal). The influence of the vac r inia virus on the development; of anti-Eberth antibodies, hemolysins and the body weight of e-.porimental animals was studied (N. Ca- jal et al.); the evolution of vaccinia and antivaccinia immunity in the rabbit under the action of cortisone (N.Ca- jal and Y.Copelovici); the action of ionizing radiations on experimental vaccinia and immunity induced by this virus (S.Mateescu et al.); the influence of vitamin C and cortisone on vaccinia virus cultures in the embryonated egg.(C.Fopescu); investigations were made on the development of influenza RAI antibodies in animals previously inoculated with the vaccinia virus (D.Sarateanu) and the morphologic aspect of the virus in the electron microscope (St.Dumitrescu and N.l.Ionescu). in zona zoster, the morphology of the virus and histopathology of the skin lesions were described (St. Nieolau et al.) and the question of the unity or dualism of zona and varicella discussed. In fowl pox an important contribution was brought by the investigations of the institute to the immuno-biologic relationship between the various virus strains (V.Tomescu et al.) and the aetailed study of specific inclusions. The Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 vaccine prepared was currently and massively applied in tile 1-1. -. . DeIa ~,- I st al, w apec1iic _k las ions were car.LJ. d -,)U4 .At3la~a33a~iu et a1'.) in measles anu. German measles, the morphology of the virus was studied on smears stained by pnea?sl e (St.Nicolau et rel.); the morphopathology o";qs:t iieasies encephalitis was likewise investigated (G.Marinescu et a10 and German measles transmit yea experim. ita1 to Fah : r l; it (NoConstantinescu et al..). Attempts were made to prepare an anti-measles vaccine with the virus inactivated by immune serum (St.Nicolau at al. end comparative studies were carried oy.it on cultivation various mumps virus strains in a.ifferent celis, in vitro rI.Adevca ana M. Cepieanu), ? aut,ochionous strsins were isolated (M.Cepieanu at al.) ana vaccine now being prepared accoraing to the Katz-Milovanovici formula (I.Aderca and M.Cepleanu). in swine plague, the investigations had in view especially specific prophylaxis (V.Wynochradnyk,S. Mihail~g at al.; V.Tomescu at al.); the comparative value.of vaccines adsorbed on aluminium hydroxide and those inactivated by crystal violet was investigated (S.Mihaig at al.); the duration and intensity of immunity conferred by egperimentai vaccines were established; vaccines were prepared from organs other than those usually employed and a vaccine using .Lapinized virus. was tested (S.Mihaita et al.); the reversi- bility of the lapinized virus (L.yascu) and the distribution of the vaccine virus was investigated (izheorghiu) The results of Rumanian investigations in this viral disease were summed up by i?Gheorghiu, S.Mihaiita and co-workers in a monograph published.,by the R.PoR.Academy. For their important contribution 'to the problem of swine plague, V.Wynochradnyk was awarded the State Prize for 1954. In avian pest and pseudopest, numerous virus strains were isolated and typed (C.Su rdan at alitl.Suhan et al.); interesting immunomprophylactic studies were conducted and specific vaccines were prepared and massively Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 T l -it tie country (So %pplie , w' h the best results hl-X3 ,gI ,,i:1.aci et al.); morphopat o4.`agic study OT ,tc . - - ..bryo ie:Aons (Serb ,esc' . et ?,) an avian pest a.mti *,erum was T)t?9pared in turk y,s. (I Suha?i et al3) A b.i.s4opat oi;ugic study or the nervous sys.-Rem 3n m?n:e exJe rinienta-L-L.y infected with an avian pest strain speci.:.~ Jy adapted to this animal spL,cies (R0Porttoc;a1_, and N0Cajal); t;:; investigations had In view a comparative study of the value of antipast vaccines prepared with live modified viruses (i.Suhaci et al.) and the distribution of the vaccine virus, For his exceptional contribution to the problem of specific prophylaxis in avian pest, l0Suhaci was awarded the R.P.R. State rrize for 1954. In the problem of viruses and tumors., an importan e, contribution was brought to the to.Llowing questions : the oncolytic aouion of eeitdin viruses (Sr 0!' ca4au, EeLVastac, B?Anagnoste), the correlation between viruses and cancer (S0Nicolau), the study of :Ehrlich tumors, proving their viral etiology (.E?Nastac et all.), the evolution of Gudrin sarcoma in animals previously infected with the vaccinia virus (N. Cajal, E?Nastac), the combined oncolysing action of certain viruses and cytostat cs or narcosis (O Cost.chel and E?Nastac); the action of certain 'cheiating products on experimental .r.ors (G ?Baimug et al.). An agent with "double antigenicity was obtained by inoculation of adenovirus type 3 into Ehrlich's ascites ~: .rcinoma; the action of ribonucleic acid extracted from ,rious viruses on several experimental tumors, was investi- gat,edm In the problem or the viral-etiology of human and animal leukemias, atrpmpts were made at isolation of a viral agent from the blood and organs' of animals or patients suffer- ing from leukemia; the pathogenicity of murine leukemic products from AKm mice for the rabbit, guinea pig, mouse, was demonstrated; anatomo-pathologic lesions were described and their transmissibility by serial passages (EjNastac,B?Anagnoste Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 XVI et al.); in human leukemip our investigators showed the pathog6nl..city of Jiurnan leuks.. ,zap; products ';ar the white hybr: a mouse in zwb .ch a serially transmissible disease with histo? pathologic modifications was producea (E.Nastac et ale). At the aame time, studies were carried out in the electron microscope and on the evolution of experimental leukosis in animals irradiated with radioactive isotopes (N.Cajal et alp),, Two virus strains were isolated from cases of, human reticulo-sarcoma; the disease transmissible in series in the embryonated chick egg was studied in the optical and electron microscope; eJ.e-"gatary bodies were observed in lesions of the c,kaoor:o-allantoic membrane (N.Cajal and M. Cepleanu). In Hodgkin's disease, several agents transmissible in series were isolated in the embryonated egg, producing lesions at the level of the embryos and cytopathic effect in tissue cultures. Their reaction to physical, chemical and biologic agents suggests the viral nature of the isolated strains, that form the object of ample experimental morpho- logic and serologic investigations (A0Schachter, N.Cajal, M.(;ep.Leanu and Y.Sorodoc). Acad. St.Nicolau published a monograph dealing with the correlation beuween cancer and viruses; and N. Cajal and E Nastac wrote the chapter on viruses and tumors in-a treatise on oncology published by bhe Editura Med.icalap Apart from these important studies, the inframicro- biologists of the institute have also carried out numerous investigations in other problems, such as ; infantile sueta miliaris (St.Nicolau et al.), hydropigenois neuroviroses of fish (St.Ricolau et al.), jaundice of silkworms (A:0Derevici), infectious anemia of horses (C.Surdan et al.), distemper (St.Nicoiau and C;06ur?dan), unapparent viral diseases, spon- taneous inclusions (St.Nicolau, R?Fortocala, N. Cajal), in infectious mononucleosis (D?Sarateanu, G.Marinescu) , infectious lymphocy4osis (N?eajal et"al., G.warinescu), Kurloff's bodies (St.0Nicolau et al,), cytomegalic disease (R.Portocal& et al.), certain eruptive viral diseases (C.Mitroiu _et al.) and foot Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 - Declassified in Part - Sanitized Copy Approved for Release 2011/12/06 : CIA-RDP80T00246AO19200640001-8 soon hA~ At tiahe'` bro'adcasting' stet Ora, t8 ev sion ystaat: on:,:= and R.iftimovici More. than 'too lectufres on ~%~rus:eis? have? Babee, by St Ni~colau )and-'3) The wor1d..of ?v use$, Eby t.C,aja1 view "S&udii si cercetari de inrramicrobio;logieft (Stud es . and investigations in inrramiorobiology), (-5895 pages) ?: Two bookie bs for the populaEizatiot~ of science b.ave., also- been published: 1) Microoes and :viruses: ,. 2) V. .(in the press); 27) Mumps, etc. The institute has published 42...aambers of the re- ,.F ,Y iser s ,a. microscope techniques in the press);. eo) 41CketG$ press); 4) Adenovirals dise;as,es. (in the press);;: ~ ) le~ctron l d i , r inr_rau.microbialO V ;(in..the. biology. 2o) ale te_nt* of. gezserr i nfratio }robiOLOgY}: ; of, special infram crobio;1,ogy; ;e2),:.Orn Ftrhosis dl.)" Elements inframicrob ology ('in t;hinrese ),;, 19), he,et.ures_, on. inf am cro,; 17) Influenz clefs on. the:,. 1959:, epdemic-; 1$) t'roblems of disease ; 1) Swine p-t;f 16) . fihey jaundice of : siaga lk worms ; Diseases transmissible to man from animals; . il) Polfiomye Otis; 12) Viral infectious hepatitis; 13) Rabies; 14) Fo.ot anRd mouth seases; 9) 1nr'ectious-lymphocyt?s;is and mononucleos;is;.. l.oY) ?/) viral hepatitiae.s,;, 8) Laboratory diagnoses, of v,rq4, d1-s: phalitides; 3) Ly?nphocytic...meningitis.. 4). Influenza; 5) Eie? menus of general l niramierobioogy, 6) cancer and 'viruses; of influenza; 2) Soviet invesi; ggtions, in. the field of ence- or are in the, press: 1) Soviet investigations ,in the fish. Thus o monographs < heave _, be?.n written and.' pub 1. she q 9 sis were likewise pub1ished.by.G.Marinescu. the works concerning mononucleosis and..,infectious.,lympnocyto- in his monograph Febra,;aftoasa. (F,oot. and.mouth; disease.) , andz:\ latter disease-,were summed up by V-eTomescu- and,,,collaborators (V Tome seu-,. et al.;) manyinteresting investigations were ,.: carried out. r Most of the..Rumanian investigations in the and mquthkxdsease, for which, an=;efficient vaccine was- prepared of science.' published` ii newspaper's` anti ?revieW6 fb - ?t~h p ;pn'iariza~t}io.,nxa: hundreds5 of scientific articles, clecklakra'tiahs tc: have been. public lecture halls in Bucharest and, the prvinc'eis,.F.. ee the percent- age of mortality and prolongs the period of incubation and the life of these animals. Larga-ly administered by intramuscular route to the white mouse infected with the street rabies virus does not influence the evolution of the disease, but seems to exercise an effect on experimental rabies of the golden hamster, 26. M,CEPLEANU, Y.COPELOVICI. Comparative study of the culture of, some' viruses' On2embryonstad-7ia; : deeiabry6hdted"eggs ^R@pett-t@aadnatoth@" S@@sion of the _ In@t T Of - Ifframicrobiology of the R.P.R. Academys July 31, 19580 The inoculation of some viruses on the deembryon- ated egg according to a modified technique, makes it possible to obtain good results with viruses that develop on the endo- derm of the chorio=allantoic membrane (influenza virus, mumps virus, Newcastle disease virus). This methos.3 can be used for electron morphologic studies, for investigations on the dynamics of virus multi- plication, for the study of the effect of. certain substances on virus multiplication,, etc. Besides being economic, this method. also has the advantage bf removing the individual biologic factor of error. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 17 27. G.DANIEI,ESCU. Study of interference in the field of virology.: St.oerc.inframicrobiol.,microbiol. , para2itol., 1958, 9, 2, 223. The author's report on her dissertation for the -title of candidate in medical sciences. In the first chap- ter, many bibliographic data are given in connection with this phenomenon. In the original part of the work, the author describes-the results obtained in the study of the phenomenon of interference in experimental infections caus- ed by the herpes, influenza and street rabies viruses, us- ing as interfering and excluded agent the same virus ino- culated by two different routes at given intervals. The data obtained led to the following conclus- ions ; A weak encephalytogenic strain of herpes virus, introduced by intravenous route to rabbits, can interfere with the same virus inoculated later into the brain. The phenomenon of interference is manifested by a prolongation of the incubation period by 4?5 days in the animals experi- mented upon as compared to controls. Inoculation of the influenza PR8 virus to mice by intraperitoneal and intranasal route brings about interference manifested by inhibition of virus multiplication, demonstrated by the hemagglutination reaction. The fixed rabies virus, inoculated by intravenous route to rabbits confers a certain degree of resistance to the street rabies virus, inoculated into the sciatic nerve after 24 hours. 28. M?IANCONESCU, I.ADERCA. Technique of cultures on human amniotic cells, St.cerc.inframicrobiol, microbiol., paratitol,.1958, 2, 4, 429. The economic advantages of cultures on human am- niotic cells are shown as compared to monkey kidney cell cultures0 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 18 The cultures are obtained by trypsinization of the amniotic membrane, with an output of 9-11 x 106 cells per gramme of tissue. The best culture medium investigated was Hanks solution with o,b5% hydrolysate of 79% lactalbumia, 20% calf serum and 2oo 2aM 1% glutamin. 296 ACAB.ST,eS.NICOLAU, I.MIRZA. ability of viruses in antiviral veecines stadted:'.b ''~WUS:,Gf` ion( In an anti-viral vaccine, the chemically attenuated In the experiments men- titer is of paramount importance. tioned, the fixed rabies virus inactivated by formol conden- trations higher than 4 %o becomes non-immunizing and also indifferent to pre-established conditioned reflexes in rats. In concentrations lower than 4 %0 ,formol respects the immunogenic property of the virus and apsets the pre- viously established conditioned reflexes. Similar experiments results. carried out with the influenza virus yielded the same re It is reasonable to expect that up to 4%o, formol maintains the virus alive, preserving its property of up- setting the preestablished conditioned reflexes and its immunologic potential. A formol concentration exceeding 460 causes death of the germ-and ipso facto failure to react tb conditioned reflexes and abolition of immunologic properties. The sensitivity of the conditioned reflexes test for determining the ' ,Viability of germs in ananviral vaccine prepared-with attenuating chemicals is clearly revealed. 3o. R0PORTOCALA, V.BOERU. Electrophoretic micromethod on 1 16. agars Biochimia A,plicatA, 1958 9 5-+ St.cerc..inframicrobiol, 1958,v.29 2, 167. By applying a layer of buffered agar on a micros- cope slide, it is possible to obtain an eleetrophoretic micro- Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 19 method that yields a curve which can be superimposed on those obtained by the classical electrophoretic methods. Accurate electrophoretic determination with minimum amounts of serum can be performed by means of this micromethods 31. R.PORTOCALA. Electron microscopy in cyto-pathologic investigations on viral diseases.. Microbiol., parazito1 ; epidemiol ',' 1958-T `-193- A brief survey of the present-day data on the techniques employed to obtain thin sections for the electron microscope. The principal results obtained by these methods in the study of cellular lesions caused by inframicrobes are succintly listed. 32. I.ADERC` 1, 1,;.IANCONESCU, M.NACHTIGAL. ];or, to obtain human embryo cultures by trypsinization, St.cerc.inframicrobiol., 1959, lo, 2, 187. The authors describe in detail the technique employ- ed for preparing human embryo cell cultures by trypsinization,, One to three months total embryos .;.ere used. The output of trypsinization was 4o x 1o6 cells per gram of tissue. The following nutrient medium was employed : Hanks and Earle so-I lution + calf serum + glutamine solution + lactalbu .i.ne hydrolysate. The cultures develop quickly forming a conti- nuous layer of fibroblasts, which can be inoculated between the 4th and 6th day after seeding the cells. 33, I.ADERCA,''..IANCONESCU, U.NACHTIGAL. Study on in vitro cultivated human cells. Methods and cytologic stud,Y. Consfatuirea Interregional. de morfologie, Tg.Mure*, Jan.18-19, 1959, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 2o The techniqueSof tissue and cell cultures devised for inframicrobiologic investigations have led to the deve- and microbiology. The cultures obtained lopment of cytology out extremely by modern methods make it possible to carry minute cytologic studies. A description is given of the technique employed for obtaining human cell cultures from human embryos and human amniotic cells. The authors des- cribe the normal cytologic aspect of the Cultures nddi- cuss certain cytologic problems linked to the development of cells in cell cultures. 34. IJADERCA, M.IANCONESCU, M.NACHTIGAL. Susceptibility of humanbr o t sini.~ed~ell~`;~':;3~3d~ ReV;SOt Med'., ' 9398. The human embryo, a readily obtainable material, prepared by trypsinization, has a high cellular output per gram tissue and develops rapidly. Human cambryo cell cul- tures are extremely favourable i. the growth of poliomyelitis, Coxsackie, herpes, vaccin.a viruses and adenoviruses, yield- ing a constant cytopathic effect, 35. N.CAJAL, O.BURDUCEA, S.MATEESCU, G.MARINESCU, MTCEPLEA- NU, Y.COPELOVICI, The evolution of some experiment- al viral diseases Wender the action.. of tadioactive Phosphorus and iodine ( P and-1131). Report read at the second'Congress of Hungarian-micro- biologists,. Budapest, September 22-25, 19590 The morbidity and mortality of animals infected with various viruses (influenza, Coxsackie, poliomyelitis, rabies and herpes viruses) and submited to a treatment with radio- 11 active isotopes ( P32 and I ) is clearly increased and the average perio,i of incubation j.d survival reduced in compa- rison with controls. Biologic titration of the presence of Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 21 the respective viruses showed a more intense multiplication of the germs in the organism of the irradiated animals. Under the action of beta and gamma radiations, some viruses,such as the poliomyelitis virus, type II (MEF1), may induce experimental infection following inoculation by routes (peritoneal or subcutaneous) other than those usually employed. The Coxsackie virus (A2), pathogenic only for the new-born mouse, determines in the adult animal under the action of P32 or 1131 a lethal paralytic disease. Moreover, under the action of internal irradiation, the virus persists in various organs and tissues a longer period of time than in the control animals. 36. M.IANCONESCU, I.DERCA. Susceptibility of human embryo cell cultureA obtained by trypsinization, to infec- tion with certain virusese St.cerceinframicrobiol., microbiol.,"parazitoi., 1959, lo, 2,193. Human fibroblast;: cell cultures obtained by the trypsinization technique previously described by the authors, were inoculated with a great variety of viruses, in view of studying the cytopathogenic?ef-fect. The following viruses were tested : poliomyelitis type I. II and III, ECHO virus type 1-3, 5-9, 11-13 and 19, A.P.C. virus 1-k and 6, herpes virus, influenza, epidemic mumps, rabies, swine pest viruses, obtaining a cytopathogenic effect with all the types of polio- myelitis, ECHO, A.P.C. and herpes viruses. The morphology of cellular degeneration is different and characteristic for each group of viruses. No cytopathogenic effect is obtained with the rabies, influenza, mumps and swine-pest viruses. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 22 37. M.IANCONESCU, I.ADERCA, N.CAJAL. Cytopathologic modi- fications produced by -viruses .r " all cultures: ConsfAtuirea interregionala de "'morfo2agge^,^ Tg.Murept Jan.18-19, 1959. The authors study the various c;-,.topathologic aspects caused by the poliomyeli-;-is, herpes, A.P.C. viruses, etc. in human embryo and amniotic cell cultures, obtained by tr*psinization. They discuss the specificity of the lesions pro- duced by various viruses on "in vitro" cultivated cells and the possibility of identifying them with a view to making a diagnosis based on the cytopathogenic aspect. The investigators likewise discuss the problem of the parallelism between in vivo and in vitro cellular lesions. 38. S.MATEESCU. A new type of sterilisable lyophilization apparatus...with'a vac uffi deutce $t:~e ~ "Infra= miorobiol 1959.s-109-1:'1- 357 n A new type of lyophilization apparatus is des- cribed, sterilisable, economic, readily built.in any labo- ratory and easily handled, with a vacu= device. The apparatus comprises two parts : a double wall glass vessel to which the lyophilization viale are attached and an empty, 3o to 4o liters water-tight closed container. A vacuum rubber tube links the vessel and the container to. a vacuum pump. The vacuum pump operates only 3o minutes at the beginning of'the process of lyophilization. Drying of the biopreparations lasts 30 minutes to 2 hours. Experiments were carried out with this apparatus during 4 years. The lyophilized preparations obtained were excellent qualitatively and quantitatively. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 23 39. R..fORTOCALA, S.DUMITRESCU, N.I.IONESCU. Simplified washing technique of the preparations for electron microscopy by dialysis. Comunicarile Acad,R.P.R. Sect.St.Med., 1959,,2, 11,1205. The authors developee.-. a washing technique of the preparations for electron microscopy by dialysis. The grids, covered by a formvar membrane, are put in a Petri dish, on moist filter paper. A drop of the suspension to be exa- win@-a.- .s put on this grid and washing is performed by diaj.. /1,U _InL (3 distilled water soaking the filter paper. The essential condition for good washing of the preparation is perfect adherence of. the grid to the moist filter paper. This technique has the advantage that it does not lower the virus content of the suspensions and achieves a correct washing of the preparations by the removal of electrolytes from the suspension to be examined. 40. R.PORTOCALA, N..I.IONESCU* M.ANDREESCU, S.DUMITRESCU. Use of suiphuretted - latin^!ur and palladium in the shading of preparations for the electron microscope. Comunicarile Acad..R.P.R. Sect.St.Med., 19599 29 11, 12o9, A mixture of sulphuretted platinum and palladium (at a ratio of 3:1 for the respective methods), readily evaporates and.can thus be used in the current technique of shadowing of the preparations foh the electron microscope, obtaining a sufficient contrast even for E;.:L,yer of 6 A thicYr c4 ness. The diffraction electron images showed that after electronic bombardement during 3o minutes at 8o KV, the layer deposited maintains its amorphous structure. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 24 41. R.PORTOCALA, V.BOERU0 Biochemical methods of inyesti? ga?hion in electrophoresis micromethods on agar?agar Selo Voprdsi meditinskoi himii, 1959, .~, 3100 Electrophoretic micromethod for determination of serum proteins in thin agar films on glass slides. The electrophoretic curves coincide with those obtained by the usual electrophoretic methods. It is possible to separate very small amounts of protein fractions from the serum in a short interval of time, and then to stain them with bromm phenol blue. Quantitative electrophoresis determination is obtained by means of an automatic densometric system. 42. N?CAJAL. Recent advances in virology. Microbiol., pa- razitolo, epidemiol., 1959, 4, 293. The latest advances in the field of virology are presented, particular attention being paid to morphology , biology, the methods for, isolation of viruses in cell cultures, diagnosis and prophylaxis of the most important viral diseases. The role of nucleic acids in the reproduction of viruses, controlled variability especially by recombination of ribonucleic acid and 'viral protein, host-cell virus rela- tionship, radioisotope labelling of viruses, the discovery of ECHO and AoP0C. viruses, the relations between viruses and tumors, the viral etiology of leukemia., the effect of ioniz- ing radiations on viruses, new vaccines with live viruses, efficient in poliomyelitis, measles, etc., are viewed and analysed in the light of the latest investigations in our country and abroadd With a view to recent advances in virology, the prospects of this specialty are discussed and the important place it must occupy in biology. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 25- 43-- I0SABUEL. The origin of viruses in the light of dialectic- materialism. Stud.cerc.inframicrobiol.196o, 11, 2, 307. Various theories on the origin of viruses are re- viewed in the light of dialectic materialism and the idealist theories concerning this question are combated. To conclude, stress is laid on the discovery of the role of nucleic acids which raises new aspects of the problem of the origin of vi- ruses. 44. R0PORTOCALA. The relationship between the immunogenic activity of an antiviral vaccine and its infecti- vity. Stud0 cerc . inframicrobiol 0 ,196o, ll, 3, 3650 The role of ribonucleic acid in the viruses that constitute antiviral vaccines is discusse d0 The conclusion is reached that active vaccination can be obtained only with a virus that has maintained to a great extent its infectivity through its RNA9 capable of inducing the cell to synthetize the homologous virus. The viruses that have lost this capacity can no longer induce intracellular immunogenesis. This implicitly points to the conclusion that the site of immunity in the sphere of viral infections is the cello since only within an integral cell, can RNA exert its inducing action that leads in the last instance to the generation of virus, which becomes antigen through its protein. 450 ROPORTOOA.LA. New data and interpretations in the problem. of viral multi,pli.cation~ Stud.cerc.inframicrobiolo :Ij6oa I, 49 597. The work is divided into three parts: the first part is dedicated to our present knowledge on the role of nucleic acids and particularly of ribonucleic acid ig the mechanism of multiplication of small viruses, with a simple chemical struc- Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 26 ture, true nucleoproteins The second part sums up the research work of the author and his coworkers on the in- fectivity of RNA extracted from influenza and Newcastle vi- ruses. The third part deals with the interpretation of cer- tain notions of i.nframicrobiology, viewed in the light of the latest discoveries on the role of nucleic acids in viral particles. 46. N.CAJAL? Some considerations on the diagnosis of human 4TF _41 inframicrobial diseases. Microbiolu,.iarazitole-9 opidefniol m 1960, - 5.,.. 3850 A description and analysis of the present diagnostic possibilities in human viral diseases. The authors show the use and classify the methods for the isolation of viruses in laborators animals, az-mbryonated eggs, cell cultures, describ- ing the various serologic, histopathologic and allergic me- thods. The authors point out the importance of a close collaboration between clinician, epidemiologist and virolog- ist, as well as the necessity of organizing a great number of laboratories, endowed with the equipment and biologic products for viral diagnosis, and staffed with specialists? The illustrated by a number of synoptic tables giving indications for the diagnosis of the most important hu- man inframicrobial diseases, according to the clinical phase. 47? G?DANIELESCUV- V.BOERU, I?ADERCA, M.IANCONESCU. Glucid metabolic alterations in the chorioa7allantoic membranes in .vitro: under the influence :of inter- feron? Studocercoinframicrobiol. 1961;, 12, l,1o9? Interferon modifies the cellular metabolism of the chorio-allaatoic membrane, in the sa:cse of an in ibition of glucose consumption from the medium. This modification be- Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 2-7 homes detectable after .7 house P ;d 'acz t, .24 hours after contact between the cell ';and the mod-l,rm I. e t inter- .feron. A l o w e r ,8:' 11~;IGl/tt~_ C i S l . A 4 u ~ m p ~ i t k n. ' h a s Uketwiame, ibe.ea b- served in the membranes ,pf th :fte r-f ,xy-n s- Ac e-upare:d with the controls ": i axd ~Afte.r,add ithe wires. etfter 24 and 48 hours. it .is discussed whe.ather the obses-red bdq 4tion of glucid .metabolism .-is. not one of the e;nuess of interference or of virus synthesis inhibition under the :i_afhte:e ,of late r- .feron. M BA888.. 70 years :since the discover r of the ,basis Of ,sero- the:rayys 1a882=1959) . Stud,.:ee.rc ; 4fr crob. ol.:1961, 22,, _L .143... It is emphasized that the first ,categoril.etametet on the law' of passive immunity, that stands at 'the bae.is of serotherapy, was made by Victor Babe:g in 1889, After showing in detail the stages .qf Y ator ;Babee~ s laborato2 e.xpe r m_ents up to their. f final anc.ceas ;amd ,appiic ationr of the results obtained to persons ' seoe_re 1y b~tten by wolves' Ft the Victor Babe.Q Institute in Bnchar~est fbasi.des the Pas- teur vaccination - the author sta' es that the *oment ;has come in which the priority of the discovery made by Qictor Ube, that stands at the basi of serothera ,ahou::1.d be rzecognised throughout the worie,., .,k_ee;ping account of the Aunqusst~iosable hstoric:al _facta recognized moreover by a nwlnber of scientists. 49. . A.DVICI. The imctomoete virus. Btud,eerc..infrsmicrobiol. .wig. .~ia~~e 1961. 12.9 19151:. The techniques for obtaining ncomi?Y leyte vi=ark ~%e described .and the theories concerning the mechanism ,of pro-- ' duction of incomplete elementary bodies are discussed. ~4milarly,, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 ,__,. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 28 morphologic studies linked tc the presence of the incomplete virus are presented. 50. R..PORTOCALA I..1ARCULESCU, I..SAMURD. On the materialis.t- dialeetic interpretation in the problem of'vipal biosynthesis. Cerc.filozofice 1961, 8, 1, 81, Starting from the experimental results obtained concerning the replication of some viruses by inoculation into the. live.cell of their isolated nucleic acids, the authors indicate the materialist-dialectic aspects that should be de- veloped in order to understand the process of viral multipli- cation. Different conceptions on the nature of viruses are .critically analysed and the conclusion is reached that although the fact that viruses are not living matter has not yet been demonstrated.-it may be admitted at least as a working hypothesis. 51. R.PORTOCALA, N.I.IOI SCU . The morphologZ of aucle.ic acids.. Stud.cerc.inframicrobiol. 1961, 12, 4 55740 The results of morphologic investigations concerning nucleic acids extracted from tissues or microO anisms are reviewed. It. is concluded that morphologic O arch work on nucleic acids is still in its initial stage;. 52. , ACAD. ST..S..NICODAU. The achievements. o1 .,,the, Institute of InframicrQbiology in its ten years of , activity. Stud.cere~rinframicrobiol. 1961, ,,supl. , 15. The-'report briefly reviews the achievements of the Institute of Ibframicrobiology in the last ten years : sc.ie;n- tific works,,. didactic activity, acti'Vity kinked to field necessities..,,., Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 29 3. R;.RORTOCALA. ntrestigatio;aj in t *: inftioua_r-0ie of viral. muole. c acid"." Stud.ceras.infram~.- g bio1;1961, 129 supl., 43,-- _, This, paper reviews the results obtained ' in ' inves- tigationa- carried out. on the infectivity of ribonucleic acids extracted from the influenza and Newcastle viruses, and des- '~,. o ibonucleic said egtraated.from adenovirus. The assumption is made of the biosynthesis of .viruses under the ~inf- lue;nce, of nucleic acids and the role of the latter in the mechanism of immunization. 54. L.MIRZA. The results of the investigations of the Insti- tute' of Tr~fraiffi1Cr8b'o?erSiII~Lhe.,raienf the -dent dT^aartsat.-Bestem,In-v aA,*^di6eas6s,,3s?hd^ viral I=antty: ata~fl a~ a s ab3A t ~ . 12 _8up; ; ~1T9;' The author reviews the experiments carried out by the: Institute of Inframicrobiology of the R.P.,E;Acacmy con- cerning various aspects of the reactivity of-the-Organise in. viral diseases, in terms of the state of cortical reactivity. On the one hand, the reactivity of the .oatganise , whose cortical activity has been previously modified by various procedures, following inoculation of various vites? was in- vestigated, and on the other, the disturbance in cortical activity that appear after inoculations without the intervention of an additional factor acting upon the central nervous system. 55? I.ADERCA, M.IANCOIESCU. The value of human embrryyo cell cultures for-the J.2abarat?rie h ot"'ihfram3Crc~UloTo $tud. eero infre dcrobiofigl;:-"'3 A:'l~al 127A -The investigations of the authors on cultures of human embryo cells obtained by trypsiztion of the entire 1 to 3 months old human embryo, accon to :a technique Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 30 devised by the authors, are _ ,iewed. These -cultures are susceptible to the cultivation of 51 different virus strains, - IAo c scusp- -- of which ECHO, polioviruses, etc. The authors works carried out in the Institute in which hum;a :e~ yo aeil cultures were used, such as replication of adeao-iirus type 3, the preparation of interferon, isolation of hepatitis virus. The theoretical premises that stand at the bazis of the high biological value of this type of cell culture are discussed. 56. ACAD.ST.S.NICOZAU, L.MIRZA, I.SAMUEL, T.ENACHE. A study of the immunogenic-potential of formolized rabies and influenza- vaaeines b means of the conditioned reflex method. - Stud eerc:inframicrobiol.1961, 12; supl: 175? The conditioned reflex method was used as a test for the determination of the degree of viability of.. the... Y1 ruses contained in a rabies and influenza vaccine ~-tx',~a`ka. by means of chemical agents. The investigations showed that addition of formol up to 4% in the case of the rabies virus and 1 %o in the case of the influenza virus respectively, does not impair their viability and maintains their immuno. genic potential as well as their property of disturbing previously established conditioned reflexes. Addition of formol over 4 %o or 1 %o in the case of rabies and influen- za viruses respectively, kills the germs and Mtppresses their immunogenic potential and their action on conditioned reflexes, which are not altered. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part -Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 II VIRUB$8 AND TUMORS 57 - 86 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 `-?-~ 31 AC.STeZs1`IIQI,AU. C?11ug roll >rat_~, s in the human and animal or ani?m . under. the -"influence -of._ certain inframierobial erma. Some considerations on the viral-h. othesis of cancer. Lectures. The Publish ing House of'the Chinese People's Republic Academy of Sciences, 1954-55, 79? The author reviews the various proliferative eel- lular pro'cesse's' induced in the organism by the activity of inframicrobial germs, referring especially to a number of 17 viruses with the most varied affinities : neurotropic,. epitheliotropic, mesodermotropic and pantropic. On the basis of more than 70 bibliographic indications, part of which from personal worse, new data and concrete arguments are brought for the understanding of the mechanism of tumoral genesis under the influence of known inframicrobial factors. Interpretation based on numerous morphophysiopathologic documents lends support to the conception of the viral etiology of tumors, a field of investigation in which Soviet science has carried out increasingly interesting research-works,. The problem of the inframic robial etiology of cancer.. seems to near, its final solution due to the remarcable' and fruitful effort of the Soviet investigators. paper. .Many figures (41 figures) illustrate the present N.CAJAL. Inframicrobial etiology of tumors in the light -of the latest investigations. Probleme de Infra- microbiol,, Edit .Med;,Bucuregai, 1956, 19 99. After describing the-known data in literature on the viral etiology of certain benign human tumors, and benign and malignant tumors encountered in animals, new data are brought sustaining the hypothesis of the viral etiology of cancer in general, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 32. These data show tb.- intervention of a specific virus, which penetrates into the organism already during the first days of life (probably with the mothers milk), in the production of malignant tumors. This virue is maintained for a long time in a state of equilibrium in the organism in a biophyt state. Following an upset of the equilibrium betveen the inframicro-organism and the macro-organism, a situation to which numerous factors can give rise but especially neuro- endocrine modifications, the virus regains its virulence, multiplies abundantly and enters into conflict with the organism. When the organism manages to overcome the germ by defense reactions, it becomes sterile as regards the virus; in the contrary case, the inframicrobe develops actively and furthers its oncogenic action which results in the appearance of cancer. 59. ACAD.ST.S.NICODAU, N.CAJAL. Considerations on the viral. etiology of cancer. St.cerc.inframicro- biol.,mierobiol. , parazitol., 1957, 8, 4, 493? After reviewing the last investigations regarding the viral origin of cancer, and describing their point of view, the authors uphold a personal theory on the mechanism of appearance of malignant tumors under the action of an inframicrobe. The hypothesis of, a complex mechanism is emitted, according to which an important part in the appear- ance and.evolution of cancer is played not only by the virus that penetrates at an early date into the organism and per- sists for a long time in a biophyt statetbut also by the endocrine and nervous systems. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 6e. E.NASTAG. The action of some viruses on experiment tumors in mice. and rats. St.cerc.inframicrobiol., mierobiol.,parazitol., 1958,- , 2, 213. A.study was carried out concerning the action of the fowl plague virus, strain 863, adapted to mice, on mouse spontaneous sarcoma 97. The virus does not modify the cli- nical evolution of the tumor. Inoculated to epithelioma M I bearing mice, the virue' sups ressea the graft ng faculty of the tumor, which develops slower than the controls. The same virus influences rat methyleholanthrene and spontaneous sarcoma, determining resorption or necrosis, with partial or'total elimination of the tumoral tissue, suppression of the. grafting faculty at the 3rd serial pas- sage and prolongation of the incubation period of the tumor. The virus isolated from the tumor or from the brain of the infected animal is much less virulent thtLn the original strain, Rat spontaneous sarcoma is likewise- influenced in its evolution by the swine plague virus inoculated into the general circulation of the animal, but is not influenced by the parapoliomyelitis MM virus. Ebidemic parotiditis virus, and to a lesser extent ectromelia virus, exercises an evi- dent lytic action on this tumor. 61. E'.NASTAC, h,SARATEAJU. Study of the action of the virus on Ehrlich-ascitic carcinoma. St.cerc.in- framicrobiol..,microbiol.,parazitol. ,1958, , 4,421. The mumps virus inoculated in situ exercises on the tumor a temporary destructive effect, manifested by the higher percentage of degenerative cells and by the lower number of cancerous cells per cubic mm..in the groups which received the inoculum simultaneously 48 and 72 hours after the graft. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 The mumps virus does not influence the grafting faculty of the tumor until the third passage and does not cultivate in it0 P-o E.NASTAC 0 B.FUHRER. Research-work regarding the action of certain virusea. on Lhrlich ascitic carainomao Action of the hexes virus and vaccinia viruso Sesiunea Tinere tului Accad0R.P0R. ,Avril 25 0 1958 ? The herpes virus inoculated in situ does not influence the transmissiMlity of ascitie carcinoma in mice, inowalated on the 4th day after the tumoral graft, It in- fluences however the morphology of the tumoral cell,"inhibit ing tt,, multiplication process at the first passage. The vaccinia virus inoculated on the 4th and 6th day after the graft, prevents the development of the tumor t the first passage, a fact which is also confirmed by the morphologic aspect of the tumoral cell. Neither the vaccinia virus nor the herpes virus can be recovered from the tumor.,. 65. EoNASTAC. Effect of the mumps virus on rat spontaneous sarcoma Comunicarile Acad?R.P?R., 1958, 8, ll, 12o7. Rev.8ci,Med., 19590 4v 690 The mumps virus in allantoic fluid, taken from developing chick embryos infected with this virus, exercise a lytic action on the spontaneous sarcoma of the rat when it is inoculated intravenously to this animal, The oncolytie action may be detected clinically and ontologically. The virus does not influence however the grafting faculty at the first passage. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 35 64o N?CAJAL, E.NASTAC. Study of the development of the G u e r i n .yitheliena in rats Pr9Ti-GR@A:Lr infected With- the IM44 is vi u ~Bq Staeersoimf am3arobiolfl q 1,959, 9 4g-4 14 L? R4 oSoAohI0q,oq~ The evolution of the Guerin 1'8 epithelioma grafted on previously immunized rats is studied? The grafts made on animals immunized 2o and 4o days previously9, give a higher rate of positive results, the *u ,.ors appearing soom.,r and their development being greater thanI the control animals, Sixty and especially eighty days after immuni- zation, the. tumor grafts gave a lower percentage of positive results, the tumors appeared later and they developed less than in the control animals. The .grafting faculty and the morphologic substrate are not influenced by the development of the Guerin T8 epithel- ioma in rats previously immunized to the vaccinia virus. 65. O?COSTACHEL9 EJNASTAC, BAILIE. The influence of the vacci:= nia virus associated to narcosis on t4.e evolution of Guerin T8- a ithelioa;iaa ice.-..rats. fr .ierobiolo,~..1959'~..l,o 4a-42,50 Stacercoiu? . . The vaccinia virus inoculated by intraveoc or intraperitoneal route to rats with Guerin caarcinow in- hibits tumoral growth at the beginning of its develop Deep and prolonged narcosis during 45 urs, associated to-the vaccinia virus, decrease the pro ion of metastases and the mortality rate, as compared the control group and to the group inoculated only with the vaccinia virus. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 36 66. A.AEREVI3I, AL.PETRESOU. The action of the hydrosoluble fraction of bee royal ' jelly on mouse8 - aseitogenie tumor: C:R:dea"Sacaa"fle"Ta~Soc.Biol., 1959, 1539 2, 172o. The hydrosoluble extract of royal jelly produced y Apis mellifica exercises an oncolytic effect on the Ehrlich ascitic tumor, inoculated to mice by intraperitoneal roate. This effect is proportional to the amount of the product used.and to the interval of time after infection, maximum changes being observed on the loth day after ino- culation, Oncolytic changes are to be found also in smears from the peritoneal fluid of the second passage,.in which the above mentioned product was not used. 67. E,NA TAO, B.FUHRER. Research-work regarding the action of certain viruses on Ehrlich- aseitie eareinOmaa Action of the lymphooytic'. ehorio=~me:ningitis" virus and the MM Virus*"*- -Comtic&rile.. Aead;R.P.R; 1959; 9, 6, 64.5. The MM virus destroys and prevents multiplication of the tumoral cells and suppresses the grafting faculty of the tumor when inoculated in situ 24 hours or 4 days after grafting. The chorio-meningitis virus does not influence transmissibility of the tumor. The morphologic study of the tumoral cells shows the destructive action exercised by the MM virus and the ab- sence of this action with the ahorio-meningitis virus. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 37 68. E.NASTAC, B.FUMRBR, d.SAMUEi. Reproduction of the Ehrlich. ascitie- carcinoma by acellular filtr_ St0cerc, inframicrobiol.,microbiol,,parazitol., 1959, lo, 1, 89. m Rev.Sci?Med., 1959, 4, 730 Inoculation by intraperitoneal route of an acela lular filtrate of the Ehrlich tumor reproduces the tumor in a clinically inapparent form. The amount of ascitis producqjj is much amaller and the evolutjam of the tumor much slower than that of the control tumor obtained by inoculation of total ascitis by the same route, The tumor obtained by acellular filtrate can be serially pasmaged.. The etiologic agent of the Ehrlich tumor seems to be a filtrable germ, 69. B,NASTAG, B,Ft R. Action of the herpes virus on the Croocker sarcoma in mice, St,cerc.inframicrobiol,, 1959, 10. 217;3 The herpes virus inoculated subcutaneously together with a tumoral suspension, stimulates the development of the tumor as compared to controls, The herpes virus was not recoverable 2 weeks after grafting, neither from the tumor nor from the brain of the animals with infected tumors. It is possible however that it should exist in various tissues in a "masked" forma 7o. E.NASTAC, B.FUHRER, D,SARATEANU. Research-work regarding the action of certain viruses on Ehrlich ascitic carcinoma in mice. R4v.Sci.M4d., 1959, 1,770 The viruses of epidemic mumps, parapoliomyelitis MM, vaccinia and herpes, inoculated by intraperitoneal route to mice with Ehrlich ascitic carcinoma exercise a partial or total destructive effect on the carcinomatous cells, The lymphocytic chorio-meningitis virus inoculated Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 38 in the same cond.itions.did not show the same properties. 71. O.COSTACHEL, B.NASTAC, B.ILIB. The action of ovovaccine associated to cytostatics on the evolution of Gu4rin tdaer in . rats. St.cerc.inframicrobiol., 196o, 119. 1, 31. Potentiation of the action of certain cytostaties (Degranol, Sarcolysin) on Cu4rin tumor with the aid of the"$accinia virus was attempted. The following results were obtained : . Ovovaccinia inoculated concomitantly to grafting of the tumor intensely stimulates successful grafting and growth of the graft ; o This virus inoculated on the 3rd week after grafting inhibits the small tumors-and stimulate the growth of large tumors. The interval between inoculation of.the virus and of the cytostatics is determinate : ;;motion of the ovo- vaccinia-Degranol association on Guerin tumor. The action of association of virus and cytostatics on the tumor show-Ah&t the trophic modifications produced by the viral infection have a marked influence on the action .of the cytostatics. 72. B.NASTAC, B.FUHRBR. Investigations concerning the action of certain viruses on some experimental tumors;, Stud.cerc.inframicrobiol.,196o, ll 89 and Acta virol.Hung.196o, Z. 2, 181. Using seven virus strains, the following results were obtained The'avian pest virus, the neurotropic strain 836 adapted to mice, suppresses the grafting faculty of sarcoma 97 in mice and prolongs the life of the cancerous animals, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 39 when inoculated in situ or in the general circulation. Si- milarly, it suppresses the grafting faculty and inhibits the development of mouse epithelioma MI. The same virus inhibits the development and partially suppresses the grafting fa- culty of rat methylcholanthren sarcoma. It induces partial or total oncolysis of spontaneous sarcoma, protracting the period of incubation and suppressing the grafting faculty at the 3rd serial passage. Inoculated into the organism of the cancerous animal, the avian pest virus loses its viru- lence to a certain extent. The mumps virus exercises an oncolytic action on rat spontaneous sarcoma and a temporary destructive action on Ehrlich ascites carcinoma. Associated to the vaccinia virus it does not maintain its destructive effect on Ehrlich ascites carcinoma. The encephalo-myocarditis MM virus does not influence the evolution of rat spontaneous sarcoma, but pre- vents the multiplication and destruction of carcinomatous cells in Ehrlich ascites in mice, suppressing the grafting faculty of this tumor. The ectromelia virus determines intense necrosis and elimination of the cancerous tissue in spontaneous sar- coma bearing rats. The herpes virus, inoculated in situ arrests the multiplication process of cancerous cells in mouse Ehrlich ascites carcinomatous tumor. In mouse Crooker tumor, sti- mulation of the development of'the tumor is observed after inoculation of a mixture of tumoral and herpetic suspensions. Influence of the vaocinia virus on Ehrlich as- cites carcinoma is manifested by suppression of the grafting faculty and destruction of the tumoral cells when inoculated 4 to 6 days after grafting. Inoculated to epithelioma T8 bearing rats it has an oncolytic action that is enhanced by deep narcosis of 4-5 hours and association of o"9.1.eACadOA--re ? 1 t 8 9 8950....., The e~'ierogenous hepatitis virus, examined in the electron microscope, appears in most cases W when the germs are isolated m in the form of more or less regular, small spheruleso Their average size is 9o o loo Mpg maximum size 10 m~ and minimum size 8o M/4 - When the germs are ranged in twoes or threes, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 57 they appear in a flat discoidal forme recalling up to a cer- tain extent the aspect of red blood cells grouped together like a pile of coins. The size of the sclerogenous hepatitis virus is approximately twofold smaller than that of the Botki,n hepatitis virus These data constitute an additional argument con- firming the previously established concept of the plurality on inframicrobial epidemic hepatitidesE, lo3a R0PORT00ALA. Investigations on epidemic hepatitides, Report read at the inauguration of the Institute of Inframicrobiology (July 12, 1951,) A brief description of the contribution of the Rumanian School of Inframicrobiology to the study of epidemic hepatitis, These investigations resulted in identification of the hepatitis epidemic which broke out in our country before 19489 with Botki:n&s hepatitis; establishment of the viral nature of this di.4ease by histopathologic studies, the finding of specific inclusionse experiments on laboratory animals and, detection of inframicrobes; establishment of histologic diagnosis oriterions based on the presence of triad (I = intranuclear inclusions 9 N = giant K = multiple karyokinesis); listing of catarrhal icterus together with Botkin?s hepatitis; determination of conditions in which epidemic" outbreaks appear- detection of cases of unapparent disease; data regarding the trans- mission of the disease to animals. In sclerogeniv 'epatitis the following were es- tablished :'the histo J. thoiogic appertenance 9 which defini- tely differs from that of Botkin's disease; clinical dif- ferences between the two hepatitides; application of the hemagglutination reaction; certain properties of the virus; study of peripheral blood; determination of nucleic acids in the sera of the patients. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 58 104. NOCAJ'AL, S.MATEESCU m .w , - tSS.IFVICI 9 G0POPESCU, M.CEPLEA- NU, R0CAPRARU. Distribution of the sclerogenous epidemic hepatitis virus in the human body. St.cerc.inframicrobiolo,microbiol.,parazitol., 1952, 39 1-2, 1010 B4 means of the hemagglutination and hemagglutina- tion=inhibition tests-, it was possible to demonstrate that the scierogenous epidemic hepatitis virus is to be found constantly in high concentrations in the liver, duodenum, pancreas and kidneys and in smaller amounts in the jejuno-Q ileon0 The virus was not constantly found in the stomach, large intestine, suprarenal capsules, spleen, lungs, brain, cerebellum and spinal cord ; it is usually absent in the ~- I~lfl d.esti?les and lymph nodese The sclerogenous epidemic hepatitis virus is there- fore a virus with viscerotropic affinities, but can in cer- tain cases be found in ectodermal tissues, having therefore also pantropic possibilities. 105. ACAD.STeS.NICOLLU, N.CAJAL, C.ALBU, M.CEPLEANU Cultivation of aclerogenous epidemic hepatitis virus in embryoonated eggs. St.cere.inframicro-d biol.,microbiol.,parazitol., 1952, 3, 1-2, 29. Inoculation of the sclerogeuous inframicrobial epidemic hepatitis virus on the chorio-allantoic membrane,. yolk and amniotic sacs of the chick embryo, induces after the third passage, constant lesions in the embryo, which however lives until hatching. The most interesting lesions are to be found in the liver. They become apparent three days after inoculation and become more severe as the moment of hatching approaches. Only after inoculation, inconstant, congestive, infiltrative and proliferative lesions can be observed on the chorio- allantoic membrane. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 59 After the 9th passage with antigens from the liver of embryos and from the whole embryo9 positive homagglutination reactions are obtained, inhibited by the serum of convalescents of sc.lerogenous inframicrobial epidemic hepatitis. .lob. ACAD.ST0S,NICOIAAU, C.ZAMFIR, NOCAJAL, GOBALMUS, M.SEPEA- 1W-FIRICA; -S.MATEESCU, E.TURCU; I.NICOLAESCU. "Legiofls. of-the"liver in inframidrobial sclerogenous _1112: p~tSt2~o13t~TeStS~.StaA~ad:R4P:R. Sect. StoMed;"3932s,4;^3; 105.- In the course of the study of a new type of viral hepatitis, i.e. inframicrobial sclerogenous epidemic hepatitis identified in the R.P.R. in 1949, the histopathologic modifi- cations induced by this virus at the level of, the liver were described. Investigations were carried out on 63 cases; 44 biopsies were performed according to the Roholm Iversen method and 19 necropsies; these examinations , were carried out at various stages, beginning from the first week of the disease until the 28th, On the paraffin embeded liver sections, stained by various methods, it was possible to detect, in the first phases of the disease, the presence of massive infiltrations with prevalent polynuclear and mononuclear elements; in a more advanced stage, the mononuclears became predominant. However, in this case, when the infiltration on all the sections is characterized by mononuclear cells, there may exist zones of probably recent tissular infection, which take on the aspect of acute inflamation with predominant polynuclear leukocytes. Moreover, during the initial period of the disease, marked hyperemic modifications can be observed, contrasting strong- ly with the images encountered in Botkin?s inframicrobial hepatitis. The investigations of the authors likewise show that sclerogenous hepatitis is fundamentally different from the latter. The processes of multiplicatiom,,4haracterizeA in Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 6o - Botkin's hepatitis by num roes karyokineses, are extremely rare in this viral hepatitis, in which pOeu.do-giant cells, that appear after swift amitotic multiplication, are pre- valent. Nuclear inclusions were not detected either in this stage or in a more advanced stage of the disease. The general aspect of the liver suffers greater modifications with the evolution of the disease. The glan- dular tissue has a tendency to be replaced partly or wholly in certain areas by a juvenile connective tissue. In severe cases,. with a lethal end, the sclerous neoformation'tissue may replace up to 3/4 of the surface of the section examined. Numerous newly formed bile canaliculi furrow this tissue whose hepatic origin is unrecognizable. Certain cells in the microscopic field show varied degenerescence (vacuolar aspect, necrobiosis with picnotic nucleus, achromatic dege- nerescence). The-hepatic cell often completely loses its characteristic morphologic aspect, presenting the aspect of a ghost cell. 1o7. ACAD.ST.S.NICOLAU, NOCAJAL. The latest investigations in 1 the Rumanian People's Republic on viral epidemic hepa- titidesa Rev.St.Med.' Med. Intern6 -1952a-4s-1 12- Data regarding the investigations carried out in the R0P0Ro on the hepatitis epidemic, that started in 1948, and which revealed the plurality of viral epidemic hepatitides. A description is given, particularly of the results obtained following morphologic study in the electron microscope, of the properties of the virus, the histopathologic aspect, the clinical and hematologic picture of sclerogenous hepatitis. ,Thus, the hemagglutinating-property, the action of certain physical and chemical agents on the sclerogenous virus, its cultivation and the alterations produced in the embryonated egg, the distribution of the virus in the human organism, hepatic lesions in the course of the natural disease, the clinical and epidem.iologic characters of sclerogenous he- Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 61 patitis, and the hemommedullogram aspects are dealt with in detailo The data obtained following the latest investiga- tions in the RbP?R?. establish the characteristics of sclero- genous hepatitis and differentiate it from the other known hepatitides, thus lending support to the plurality of viral epidemic hepati.tidese lo8? 'ACADOST?S,NICOLAU, N?CAJAL? Etiologic ? histopathologic and itmunologic data in' epidemic viral-he Pa .Per read'at the-"Rumanian Soviet-Medical D s" 17 April 19524 A presentation of the known data in literature, starting with the year 1885 when Botkin emitted the hypothesis of the infectious etiology of catarrhal icterus, with special stress on the etiology, histopathology and immunology of the disease, The classical findings are completed with the re- sults obtained by the authork, which evidence the plurality of viral epidemic hepatitideb? A description is given of the characteristics of Notkin's hepatitis and especially of scle- rogenous hepatitis,-a nosologic entity recently identified in the ROPOR? Stress is laid on the differences that reside particularly in the histopathologic picture (the presence of the inclusion-analeole-karyokinesi,s triad in Botkin' s hepa- titis; the absence of inclusions and abundent proliferation of connective tissue in sclerogenous hepatitis) and on the a .sense of cross immunity? Data regarding the properties of the virus and experimentation are likewise expounded, special mention being made of the results obtained by the authors in their investigations& Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 62 109. N.CAJALfl S.MATLRSCU, G.POPESCU. LL.LISSIEVICI, M.CEPLEA- - Det a obtained by means of the hemaggl utino- inhibition test in' epidemmie " sclerogenous hepa e.~r.~.rir ieca titiso St?core.inframierObiel;miCrcibial.,pa- raZitol., 1953, 4, 1-2, 67. The hemagglutino-inhibition test with the serum of hepatitis patients (1,oo4 cases), using as antigen liver emulsions from a man who died of this viral disease, was positive in 81.38% of the cases studied during the height of the disease. A titer higher than 1/4o is considered positive. A--number of 238 tests Were performed with the sera of persons in contact with the hepatitis patients, yielding 428% positive results. In 382 healthy persons, positive res!)'Its were found in 12.82% of the cases, which is accounted for by the occult immunizations in the course of the clinically apparent disease. Hemagglutino-inhibit- ing antibodies were experimentally revealed in guinea pigs and rabbits, inoculated with the hepatitis virus. llo. N.CAJAL, EL?LISSIEVICI, S.MATEESCU, G.POPESOU, Y.COPE- LOVICI,'RoCAPRARU. Duration of elimination of the 'epidemic- hepatitis virus - in patients and. coivalesef~ts~, -8t;eeio:inframicrobiol.,micro- biol.,para2itol., 19539.4o 3??4, 2'71? The duration of virus elimination in 445 pa- tients and convalescents of epidemic hepatitis was studied. The presence of the virus was tested in the feces and urine of 219 patients, only in the, urine of llo patients and in the naso-pharyngeal washings of 116 patients. The examina- tions were repeated several times in 51 patients, in order to establish the evolution of the specific elimination of the virus9' The hemagglutination and hemagglutination-inhi- bition reactions were performed with centrifuged emulsions Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 _63 off{,Bg~,rne^_and,`nasopharyn_geal washings asanetgens; lPor,~theehemaggl,ut nat on-inhibition te's:t.s.tandnrd anti _Rotk n wand axiti sclerogeriottsf1serra tweje)gusedo The investigations carried out showed that the ' l .. ..Y-- - \t .n++.', t- 'i!f a. _. .>.lfl' as y}R>-,O Mr .- (? hepatitis-;virus is~ constantly,--elim hated through the feces, more seldom through the urine. and only exceptionally in the xiasoApha ^ n@;ea~l~ se'eret3"ons'od `Eid? nation' of --the, virus may `take? pl=acedruring`the 4diseas?'e?:up~ toy a'ive'~moment= mot's or Tess? diet tit"from- he moment oaf acl`iri c'al recover a ~n 'c"er'tain cases the vibrus,`~diDi' st lwl ?be e1 m3Hated a ter' ht s1- e riod (acco di'rg rto -t'he I inver - igati'ons` of' 'the f'aizthors` tip "to 255 days). Flimin'ation? of th*evirus after' "cliniea`l~ r'r. =r`e.c owe ' ` -demdnstraties'tatar the' patirei%tcondred as healed, cos " ':trues' Yto? shave '?iHbux"" s f -1 Re f?ort hepatitis. .ogic alterations inhuman embryo cell culC;sres obts3nfd by read at the Interreg. iO il-:.C.6SLferefic6'o>Q !epidemic hepatLt~ia.:c8inaia,: Oct?ber X21=P2 19;60.. A .study was carried out on the ![dtol strafii," the assumed .agent of epidemic hepatitisj, isolated by .Kulaelka in Czechoslovakia' from the viewpoint of its' cultivation.in va- rious cell cultures, laboratory animals and embryonated egga. The authors describe the characters of the m64ho- trypsinizaton'and'in the organs of inoculated @riimals. -1770 ACAD:ST.S.IJICOLAU; '`N.CAJAL. t1oz s - ?r'th8' ine`tftute' O: micr'o'bial e de' c e& it s o`l 'r9: ew i . , AsaP14. y :... Mhe, !results~ ,.,obatained, tby the . investigators of the . stit.ute of Infrramicrob.iology An the field of viral.,Ipl(eaic h:patitis during the 1.949-1961? period are ,described. S O.ZAVATF . N:CONSTANTINNSCU.. N..BIRZUj, C.OANA, GH..PANAI- TFBCU, AL.MIHAIL. Infectious virus henat~? i 8' EI:V.?H) within ,the _.:faaily env. .ronme:nt...8tud The familial .incidence of h .V J. bavas `" rives3t gated over r-a period of six years `(953-1958) three towns' of about 25o.ooo inhabitants each, situated in different geographical areas of the, R.?-R. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06 CIA-RDP80T00246A019200640001-8 - 1o6 Out of 5,468'families in which I.V.H. oeeured,, the disease was confirmed to a single subject in 95 per cent of the cases. In 1.8 per cent or the iaruilies, tine secondary cases occured at intervals ~ of o-6o days following the first case'while in 3,2 per cent of the families several cases occured at long intervals ranging from two months to five years. In 151 families in which secondary cases appeared within o-16o days, 74 per cent of these cases involved children,, -.:the possible intra-familial source . of infection being an ini- tial::..case concerning another child. In contradistinction of tha::group of families in wich hepatitis exclusively ocoured among children, in the group in which the disease involved adult subjects, the incidence rate of families exhibiting se- condary cases within o-6o days was much lower, i.e, of 18,6, lo.9 and 16.9 per cent respectively. higher during the cold season of the year, corresponding to the.autumn-summer peaks of I.V.H. A series of general prophylactic measures manly concerning the o-14 year age-groups as well as the prophylac- tic use of gammaglobu.lins are required. The.incidence rate of secondary cases within the family is The incidence rate of secondary cases among.chil- res is of 4.8 per cent, as compared to o.6 per cent in adults. .179 P.ATHANASIU, N.CAJAL, M.IALOMITEANU, M.ANDREESCU, 0. SFERDIAN. Comparative investigations on liver and muscle aldolase in epidemic hepatitis sera. Stud-.cerc, inframicrobi'ol.,1961,' 12 supl.,295 ...and Rev.Sci.M4d., 1961, 3o4. Determinations of fructose-l-monophosphate aldo- lase and fructose-1.6-diphosphate aldolase in the sera of loo psr- tients suffering from virus hepatitis revelated the presence of Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80TOO246AO19200640001-8 increased values up to lo-4o u and 2o-8o u respectively, as compared to o-8 a and lo-2o u respectively in normal subjects or in those suffering, from other liver diseases. Since fructose-l-monophosphate aldolase is exclu- sively formed in the liver, simultaneous performing of both tests makes it possible to distinguish this enzyme from muscle aldolase. Nstimation of the two results may yield more com- plete'informa-tion concerning the diagnosis of virus hepatitis. l8o N.CAJAL, O,MITROIU, C.BARBU, O.CONSTANDACHR, M.POPA. Comparative study of hemaarglutinatiou reactions with` the red cells of MRhesus and of one-da-vi d chickens in epidemic hepatitis -Stud* cero'`infga microbiol.,1961,-12, sup1:; 301. The results are reported of a comparative study of hemagglutination reactions performed with the sera of patients' suffering from epidemic' hepatitis or other viral diseases'an*'the red cells of one-day-old chickens and of M. Rhesus. The figures recorded by the present authors in cases of epidemic hepatitis are lower than those reported by other investigators, Altough these figures are definitely increased as compared to those recorded in normal subjects, Vz.ey cannot be used for diagnostic purposes since similar- increases occur.in other diseases as well. Hence,' these tests a ve of little avail for the diagnosis of, epidemic hepatitis as prevailing at'present in the Rumanian People's Republic. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06 : CIA-RDP80T00246AO19200640001-8 .;.~ Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 181 ACAI FT NIC T:.AT` STROE G ~SC'J GR, LAZAR:i;SMT r Herpes and. herpes parae3mmunity induced in man bypoliomye- litis. The possibility of paraspecific prophylaxis in poliomyelitis by means of experimental herpe- tizationa, St.cerc.inframicrobiol., 1950, It 1, 1210 After a review of various cases of paraimmunty reported in viral diseases, the facts.that point out the existence of para-immunity between herpes and poliomyelitis are discussed. An experimental study was carried out, by inoculations of the herpes virus on the skin of paralytic poliomyelitis patients and former patients, in order to in- vestigate if an increased paraimmune resistance to herpes is built up. Inoculations performed on 55 subjects showed that starting from the second month after the onset of polio- myelitis an increased resistance to the herpes virus exists, the number of positive inoculations decreasing from 72.7 % to 26.1 % in the second month and 4% between the 3rd month and the 7th year after the poliomyelitic infection. The authors propose to investigate if herpes is followed by an increase of resistance to poliomyelitis and if herpetization could constitute a method for combating poliomyelitis. 182, ACAD.ST..S.NICOLAU, T.HORNETZ, R.DINU, S.MATEESCU, M.SE- PEANU-FIRICA. A histopathologic study .of acute anteriorpoliomyelitis;.changes in the peripheral and central sensitive systems. St.cerc.inframicro-. biol. microbiol.,parazitol., 1951, 2, 1-4, 131. Diffuse changes of the glial reaction type, with neuronophagia, inflammatory infiltration or necrosis, were ob- served in the spinal ganglia, in the posterior horns of the spinal cord, in the Goll and Burdach nuclei, in the nucleus Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 109 arcuatus,~'in the locus coeruleus, in the roof nuclei and cortex of the cerebellum, in the thalamus and in certain zones of the cerebral-cortex. 183. N-.CAJAL, EL.LISSIEVICI-OPRESCU. Investigations on sego- neutralizing Poliomyelitis II Clansing) antibodies in the inhabitants of Bucnerest. Third' European Symposium of Poliomyelitis, Zurich, 1955, 63. St.cerc...inframicrobiol.,microbiol.,parazitol., 19569-2, 1-2t-'570'. A.'..study on .the, presence of neutralizing antipolio- myelitis II (Lansing) antibodies in lo8 persons of different sexes 'and: ages in , BucaareS'y, established that these antibodies are to be found in a high percentage in new-born infants, dis- appear. at the =age of". 6-12 months, after which incidence, `in- creases, attaining a~maximum titer at the age of 2o-3o,.after, which it dec"reaies `again, It was not possible however`. to define the;'nature of these antibodies ; they are either type or group specific or non-specific - paraimmune.- The investigations carried out showed however that a close coitmelatjon can be established between the presence of these. antibodies and receptivity to, poliomyelitis, the morbidity rate being proportional to the number of negative results obtained'in each aige-group. 184; N.CAJAL, N.DRAGANESCU. The action of hargactyl in mouse experimental poliomyelitis with virus II (Lansi)..Lucrarile cele de a II:a Sesiuni gtiin- Vifice a IO?F,, Buc., 13=-15 aprilie 1956, 9 , A study was carried out in 3 experiments on 84 mice inoculated by cerebral route.with poliomyelitis virus' 'type II (ansing) on the evolution of the experimental disease un- der the.action.of Largactyl, administered daily by intravenous Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 110 route, starting from the day after infection (5 . mgr ,per kilogram body weight). The experiments showed that, in comparison-with the controls in which mortality was loo%, mortality was rela- tively low (85%) in the treated animals and incubation longer (14 days as compared to lo days in the controls). The diffe- rence.was more evident in the groups inoculated uitj 10-2 virus dilutions. 185. G.MARINESCU, N.DRAGANESCU, I.TURCU, I.FRIDMAN,VCIUREZU, P.ROMAN. Interstitial . pneumonia. in 'poliomyelitis. Presse Med., 1956, 64, 94, 2207. The present paper deals with 62 cases of polio- myelitis, most of which with ascending forms, followed .up during the last 6 years. The macroscopic examination showed:' 19 broncho-pneumonia, 22 marked congestions, 5 pleuro-pulmonary hemorrhagies, 1 pulmonary collapse, 1 massive emphysema, 1 pulmonary tuberculosis. The microscopic examination revealed broncho - pneumonic foci in 11 cases, typical interstitial pneumonia An to cases (3o%), mixed pneumonia (associated interstitial pneumonia) in 7 cases, ateleotasis processes in 3 casesand hemorrhagic processes in 2 cases out of the 33 cases examined. The interstitial pneumonias were more frequent and wide-spread in small children who died during the first week of the disease, although the most typical lymphocyte infiltrations were to-be found in children with a longer period of survival. Rich in- filtrations were likewise found in the cases with more intense interstitial myocarditis and processes of 'perivascularitis in .the nervous system. These infiltrations seem to be due to the polio- myelitis virus, either because of its particular viscerotropism or its presence in the blood during the first - part - of `the'.di- sease. The action of the regurgitated gastric fluid and aspired ?bucco-pharyngeal secretions may also (be:considered:as,.adjuvant Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 lii factors. Stress is laid -on the importance of interstitial pneumonia as an aggravating factor in evolutive poliomye- litis. 186.. N.CAJAL. Present stage in anti.. olio elitis vaccinatio , "Viaita Medical." 1957, 3, 31.0 This paper is a brief survey of our general know- ledge on the protection and efficacy of the Salk vaccine. Stress is laid on the necessity of continuing investigations in view of improving this first effective vaccine, since the protection conferred by this vaccine with an inactivated virus is not complete. In view of the results obtained by,various investi- gators, in experiments on live, modified poliomyelitis viruses, .and the mechanism of immunity in viral diseases, the future of specific anti-poliomyelitis prophylaxis seems to belong to vac- cines prepared with live, modified viruses. Under the conditions of our country, and on the ba- sis of recent serologic investigations carried out, it is be- lieved that vaccination of children against poliomyelitis should be introduced as soon as possible, as a compulsory me- thod of prophylaxis. 187. N,CAJAL, EL.OPRESCU, G.FOPESCU. Investigations on neutra- lizing antipolio velitis II d,ntibodies, St.cerc. inframicrobiol.,microbiol.,parazitol., 1957, 8, 2, 2o1. Comparative seroneutralization reactions with the Lansing poliomyelitis virus and the herpes virus in persons without poliomyelitis in their antecedents, showed a frequent parallelism between the titers of serum anti-herpes and anti- poliomyelitis type II antibodies. Additional investigations carried out with the sera of dogs plead in favour of the exis- tence of other viruses with antigenic functions, common to those Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80TOO246AO19200640001-8 112 of the Lansing poliomyelitis virus.. The data obtained showed that some of the posi- tive results obtained in the seroneutralization test with the Lansing virus in persons without poliomyelitis in their ante- cedents should be interpreted with a certain caution. On the other hand, the fact that this reactivity to poliomyelitis and the frequency of antipoliomyelitis,antibodies are inver- sely proportional, fully justifies continuing researches on the antigenic relations between the Lansing virus and the other vi- ruses, in order to complete specific antipoliomyelitis prophyr laxis with a non=specific prophylaxis of the herpes type, a method advocated by the Rumanian school of inframicrobiology. 188 C=Q11AxINESOU, I.TURCO, AJ.FRIEDMAN, V.CIUREZO, N.DRAGA- ,NESCU. Poliomyelitis myocarditis; Minerva Medica, 1957, 48, 49, lobo. Involvement of the heart in poliomyelitis is an eventuality to be feared, as it aggravates the prognosis. Circulatory, degenerative and especially inflammatory le- sioxis stand at the basis of this affection. Eloquent micro- photographic-documents illustrate this paper. Almost half of the 52 subjects who died of poliomyelitis, exhibited im- portant cardiac lesions, which favoured or were even the cause of death, 1890. GGMARINESCU, I.TURCO, V.CIUREZO., A.FRIEDMAN, N.DRAGANES- CU, Poliomyelitis myocarditiis (anatomo-clinical stud .a) La Semaine des H8pitaux, 1957, _2o/3, 3300 Study of 62 lethal cases of poliomyelitis - micro- scopic examinations were performed in 32 of these cases - revealed the existence of a typical acute myocarditis in 46.8 per cent of the cases; in 13 cases myocardiac and pericardiac Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 113 lymphopolyblastic infiltrations were prevalent and in another cases infiltrations with polymorphonuclears were more accen- tuated.. The highest incidence and the greatest amount of lesions were as a rule encountered in the first days of the disease (the 4th and 6th day) in adults and older children, in cases accompanied by pulmonary complications (interstitial pneumonia, broncho-pneumonia) or in cases with intense le- sions of lymphocytic perivascularitis involving almost the entire nervous system; these lesions were likewise encountered in several cases that appeared during the last two epidemics that broke :.out. ,_in r , ;ix1 1953 6hd 1055, Cardiac insufficiency that accompanied the ner- vous disturbances was the decisive complication in at least 3 cases. With a view to the pathogenic interpretation of these. cardiac lesions, the authors discuss the various factors that may intervene alone, simultaneously or successively: tropism of the poliomyelitis virus,, extension of the lesions of the nervous system, pulmonary complications,- superimposed infections, biochemical disturbances, the stage of the disease and age of the patient. Some of the properties of the polio- myelitis virus and particularly its cardiac tropism-represent the most important elements in the onset of poliomyelitic myocarditis, 19o. I.ADERCA, M.IANCONESCU, N.CAJAL. Cultivation of the polio ym es litis virus on human amniotic cells Morphologic changes. St.cerc.inframicrobiol., microbiol.,parazitol., 1958, 994, 435, The cytopathogenic effect is identical with regard to the morphologic aspect, with all three types of poliomye- litis viruses, The initial lesions appear in the cytoplasm in the form of vacuoles of various sizes. In a more advanced Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 114 stage the lesion also comprises the nucleus and the nucleole. These degenerate and the chromatin gathers into large clumps. In the final stage, the cells are condensed in the form of round elements with a slightly acidophil cyto- plasma surrounding a.pyknosed or karyolysed nucleus. 191. ST.DRAGANESCU, A.PPTRESCU, N.DRAGANESCU, O.BOTEZ, S..CAL- MANOVICI, GHERASIMESCU. Lethal bulbar poliomyeli- tis paralysis in a pregnant woman, Lesions of the reticular substance in the mother. Anatomo-patho- logic examination of the fetal brain, St.cercvfleu .rol., 1958, 3, 265, An ana'tomo-clinical study was carried out on an acute case of poliomyelitis bulbar paralysis ending in death after 3 days, due to respiratory insufficiency, in a pregnant woman in the 6th month, The microscopic examination showed severe bulbar lesions involving the nuclei of the cranial ner- ves and particularly the formation of the reticular substance, both in the group of the medium nuclei and that of the lateral nuclei. Death due to respiratory paralysis showed however that in this case the functional damage was greater in the group of lateral nuclei.. The brain of the fetus was likewise examined and phenomena of stasis were observed, as well as perivascular oedema-and oedematous rarefaction of the surrounding tissue and even discrete hematic extravation 192. G,MARINESCU. Renal lesions in the severe .forms .of polio- . ._.., , myelitis. (Poliomvelitic n.ephritis,)Inter-regional ~r~ ~s~o~acq Rte.. o~Cli. C1 ia~li~ symposium on poliomyelitis, Vatra Dornei.,. May 22-23, 1958 - and Second Scientific session of the Insti- tute for the Study of Poliomyelitis,, the USSR Academy of Medical Sciences, Moscow, June 19-21,1958. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 1'l5 The macroscopic examination of 7o cases of polio- myelitis most of them ascending forms, showed modifications of a prevalent medullar aspect in the kidney in 58 of the ca- sese The macroscopic study performed in 23 cases re- vealed the presence'of important lesions at the level of the tubules, glomerules and-renal interstitium in 47,8 per cent of the cases. The direct action of the poliomyelitis virus on the renal cell due to its tropism and the effect of adjuvant factors, affecting the metabolism of the nervous cell (anoxia caused by nervous, vascular, cardiac and respiratory lesions) seems to stand at the basis of the etiopathogeny of poliomye- litic nephritis. The aggravating role of renal lesions in the evo- lution of certain forms of poliomyelitis is postulated. 193. GeMA,RINESCU. Hepatic lesions in severe forms of polio= Myelitis olioat el .tic hepatitis) Q (Physic -patho- logic and therapeutic deductions.)The fifth Euro- pean Conference of Poliomyelitis. Madrid, Septem- ber 1958; The third Conference of Czechoslovakian virologists, Bratislava ? Smolenice, October 14-17, 1958 The occurence of hepatitis is described in the course of severe cases (lethal) of poliomyelitis, in a pro- portion ranging between 7o 8o per cent. Hepatic damage is represented by: severe degenera- tive lesions (as a rule fatty degenerescence), inter-, peri? or even intralobular lymphohistiocyte or lymphoplasmocyte inflammatory infiltrations, hypertrophy and multiplication of reticulo-endothelial cells, discrete connective reactions and important vascular disturbances (stasis and especially oedema). Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 116 The author postulates that owing to its physio- pathogenic cop.sequences, hepatitis greatly aggravates the prognosis of the ascending or respiratory forms of polio- myelitis. Besides reanimation and the well-known treatments, a protective treatment of the liver should likewise be applied. 194.. QeMARINESCU, D,SARATEANU, E.NASTAC, E.OPRESCU,,B.FUHRER. Hepaticreacti determined by certain viruses followi inoculation into the portal vein of the -rabbit. Third Conference of the Czechoslovakian virologists.., Bratislava - Smolenice, October 14-17, 1958. After inoculation of the MM and Coxsackie viruses into the vena porta of rabbits, the presence of interstitial hepatic lesions, representing the defence mechanism of the organism, were observed under anaesthesia and laparatomy (re- action of the reticulo-endothelial cells and lymphocyte in- filtrations in the portal space.) Mention is made of the part played by the Kupffer cells in the diseases due to the MM and Coxsackie viruses.. 195. ACAD.ST.SJNICOLAU. Data and considerations on the para- immunity relationship between poliomyelitis and herpes. St,cert.inframicrobiol.,microbiol.,para- zitol., 1958, 9-, 3, 293. An attempt was made to determine the influence of the state of antipoliomyelitis immunity on experimental herpes and the influence of herpes the evolution of poliomyelitis, by means of skin herpes inoculations. The,great number of negative rbsults, starting from the second. month after the'beginning of poliomyelitis, shows an initial antiherpes resistance, enhanced by paraimmunity Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 117 brought about by the onset of antipoliomyelitis immunity. This unquestionably shows that in poliomyelitis, besides the specific refractory state to the poliomyelitis virus (immunity), there is likewise a non-specific, hetero- ogous resistance (paraimmunity) to the herpes virus. It was also demonstrated that antipoliomyelitis vaccination with the Salk vaccine induces in the organism resistance to arhythmic recurrent herpes. Para-immunity between herpes and poliomyelitis raises the problem if experimental herpetization might not be made to play a part in antipoliomyelitis control, parti- cularly where antipoliomyelitis vaccines are not available. 196. N.CAJAL, I.ADERCA, M.IANCONESCU, E.OPRESCU, A.BIRCA. Incidence od seroneutralizing antibodies to anti- , genic strains I. II, and III of the poliomyelitis virus in the inhabitants of Bucbaxw~ St.cerc. inframicrobiol., 1959, lo, 3, 281. A study was carried out on the incidence of sero- neutralizing antipoliomyelitis antibodies in the inhabitants of Bucb. st, that had not been vaccinated or had not suffered from clinical forms of poliomyelitis. Seronetttralization was performed in cultures of human anbryo cells obtained by trypsinization; 137 sera were ermined: 8o.3% presented antibodies for at least one type of poliomyelitis virus, and 19.7% were negative. Of the po- sitive sera, 31,8% were positive for a single immunologic type of poliomyelitis virus; 35.4% were positive for two types and 32.7% for three types. The younger age-group pre- sented two-stages: between 1-3 months persistent maternal anti- bodies were found in 87% of the cases, between 4-6 months incidence decreased to 14% and the antibodies were almost ab- sent (5%) between 7-12 months. Starting from the age of one year, the incidence of antibodies swiftly increased especially Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 118 those to type I. attaining loo% in the age-group of 1-2 years, a percentage that is maintained after 2o years. The authors recommend starting antipoliomyelitis vaccination at the age of 3 month"" 197? EL.LISSIEVICI-OPRESOU, The anatomo=pathologic study, of po1,el .ti.s in mice. Lesions of the reticulo- endothelial aystez, St?cerc,,inframicrobiol.,.l959, o 3, 3t -ia A study was carried out on the lesions of the re- ticulowendotheli,al system in mice, inoculated by intracerebral, route with the poliomyelitis virus II (Lansing). The material used was brain and spinal cord collected at various moments after inoculation, the sections obtained being stained by the Maim, hematodilin-eosin., Giemsa and Nicul ascu-H?tne't methods ? Analysis of the material showed that the morphologic substrate of experimental poliomyelitis in mice is represented not only by the well known neuronal lesions, but,also by endothelial and neurog1yal alterations. The latter appear at an early date - already in the pre-paralytic period and are unequally distributed, the tirus presenting an affinity for certain areas. , ;r,0 c rit?rio& O g this affinity does not seem to be of a functional order, since the lesions may be found both in the motor areas and in the areas affected to the sensitive and ve- getative functions. The. type of these changes is hyperplastic: turges- cence and hypertrophy of the endothelial elements and modifi- cation of their shape- in some cases, endothelial elements detached from the capillary wall can be detected in the ps i- vascular parenchyma,, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 119 138.N.CAJAL, Pao IANCONE$CU s I. ADEBC*, G.DANIELESCU, A.BIRCA, Study d the innideftee of antipoliomyelitis antibodies type I. II and III in nOn-vaccinated persons from rfra1 localities in the Rumanian People's Republic. St.eerc.inframicrobiol.,196o, 11,1,21. This paper, completing previous studies concern- ing the incidence of antipoliomyelitis types I, II and III in the population of Bucharest, deals-with a study in human embryo cell cultures of the proportion of these antibodies in 3 vil- lages in the country, in which paralytic polio cases were only exceptionally reported. Neutralization tests with the sera of 135 indivi- duals of different sex and ages showed that: - The degree of occult immunization to poliovirus is very high also in rural environment (74.82 per cent of sera contain antibodies for at least one antigenic type of polio- virus). - In the first years of life, a predominance of types II and III exists, especially of type III. - Antibodies type I, in contrast to.findings in urban environment, do not increase explosively, but slowly and progressively, exceeding the level of antibodies for the other poliovirus types only after the age of 12. i99 : (;AJ.A"L9ADERCA, M.IANCONESCU, E.OPRESCU, G.DANIELESCU, A. BIRCA. The incidence of antinolioelitis antibo- dies in children in the Rumanian People's Republic. Microbiol.,Parazitol., Epidemiol.196o, 1, 47. In order to establish the incidence of seroneutra- lizing antibodies to the 3 poliovirus antigen types in the in- fantile population of the country, investigations were carried out in Bucharest and in 3 villages. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 12o The sera of 219 children of different sex and ages, not vaccinated. against poliomyelitis and , which did not present in their antecedents signs of poliomyelitis, were used for neutralization tests against poliovirus types I, II and III (loo CID50), done in human embryo cell cultures. The results showed that of 219 children, 187 pre- sented antibodies to at least one poliovirus type (85.3 per cent), which proves an intense occult immunization due to clinically unapparent diseases. Thus, the incidence of anti- bodies to at least one poliovirus type, which was very high in the first 3 months of life (due to maternal antibodies), gradually fell in the following months, minimum values being found in the 7-12 months age-group; at 1 year, these values suddenly increase,. attaining the level of maternal antibodies and continue to increase with time, so that in the 13-15 years age-group no negative serum is found, In terms. of the antigenic type,, childhood is cha- racterized by predominance of type I in Bucharest,. and type. II, and particularly III, in rural environment. 2oo.d GeMARINESCU, I.DINU, I.PREDESCU, S.DIACONU, M.STARK, A1MEROVICI, S.NICOLAU, M.IVOPOLm ' Comparative electrophoretic modifications in the sera of vaccinated and non-vaccinated .noljom,Yelitis patientse The 4th scientific session of the Institute of Poliomyelitis, Moscow, May i'/-2i, 1960? Electrophoretic examination of the protein, frac- tions in 45 sera samples from 3o poliomyelitis patients, 9 of whom vaccinated against poliomyelitis-with Salk vaccine, showed in general hypoalbuminemia with increase of gamma2-globulins in the acute stage of the disease and gamma-globulins in a late stage, In a smaller number of cases (lo per cent), total hypoproteinemia was observed and in a more severe case, bypo- globulinemia with hyperalbuminemia,, As a rule, beta-globulin values were higher in the mdld. and favourable cases. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80TOO246AO19200640001-8 :121 The slectrophoregrams of previously vacinated vourable prognosis:L' `further argument, apart 'from he epidemiolog3 c"-and clinical factors, for the application of ;the live attenuated virus vaccine. In genes.l.,.gaimma-globulin values are higher in the first weeks of`the'diseasein=vaccinated patients, increasing ustill.'.fart in convalescence acid staying `level. for a long time, by-comparisoi~"w th'the non vaccinated patients,`examined"at the same stage of the disease, In a lethal case, which had been vaccinated against poliomyelitis; beta-globulin values were. s. ~.: _were' high; in " n:t>u-vaccinated patients this points to a fa- '... ijL: "2o1. ACAD.ST.S.NICOLAU; N CAJALM.CEPLEANU, I.ADERCA, N.M concerning the peritoneal,polio~virus neutralization ~rrrW~~on t, ees_t. Comun.Acad.R.P,R 1960, to 12 1157. A.pp1 cation:inexperimental poliomyelitis .of the test: previously used by S.Nicolau-. and. co-workers in=rabies, Balled the "peritoneal neutr`alization test" showed ; its utility for assessing the state of immunity induced in the mouse organ- ism by different''poliovses and vaccines (wild viruses type I, '~ fiI, III mo( poliomyel1tis The peritoneal test is highly type characteristic, and ihtensely:positive"after immunization with wild or "atte nuated" polioviruses, and negative after, attempts at immunizes- tion' with' inactivated virus ' vaccines, , of the '-Salk type.. It' is concluded that the peritoneal test ?'can re:- place the classical neutralization test, which is difficult r to apply-*indiv'idually to small-'laborat'ory animals, and thus becomes a useful method for immunologic invent gations in UXLV rV.L.tvwy Vl.l U.L5 LUb-U1-UU- a 01 Moscow*) Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 CONSTANTINESCU; M.IANCO CU. Investigations nada; lot`1o8-l: and the live Sabin virus-vaccine prepared in t 1, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 122 2o2. N.CAJAL, M.IANCONESCU, M.CEPLEANU, I.ADERCA, M.GRUTA, G:.MARINESCU, C.BABA, Y.COPELOVICI, G.POPESCU, G. DANIELESCU, 0.BURDUCEA, P.ATAANASIU, M.CIOBANESCU, Y.PEIULESCU. Investigations on seroneutralization antibodies in persons vaccinated against poliomye- litis with inactivated and live "modified" viruses. Report read at the Symposium "lo years from the founding of SANEPID" Bucharest, April 28, 1961. In 196o, the authors administered experimentally to 1,2oo children the vaccine prepared by the Poliomyelitis Institute of Moscow, with A.Sabin's-strain. Interesting conclusions were drawn from the re- sults obtained is a comparative study on the evolution of neutralizing antibodies in boo of these children and 371 sera from children vaccinated with the Salk vaccine, on the one hand, and on the other from children that had not been previous- ly vaccinated. In the latter children, without poliomyelitis in their antecedents, neutralizing antibodies against at least one antigenic type, were found in a proportion of 85.3%. Tn the children vaccinated with the Salk vaccine, the proportion of positive sera was similar (82.5 per cent) and paradoxically, in some age groups (1-2 years) even lowed. In children vaccinated (1-7 years) with the live virus vaccine, the incidence of positive sera was 92 per cent. In the same age-group, the Salk vaccine gave an incidence of positive sera of 76.5 per cent. Of 37 trinegative children, 32 (86.4 per cent) ela- borated antibodies for the type of virus in the vaccine. Virus elimination in feces was almost constant in the trinegative cases and inconstant in the children with posi- tive sera previous to vaccination., The cktldren with serum antibodies induced by.admi- ni.stration of the Salk vaccine, regularly eliminate the virus following its multiplication in the intestine, in opposition Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 123 to those immunized by occult, unapparent infections, The results point to the superiority of the vac- ciJae prepared with live, modified viruses, and its obligate application on a wide scale, 2o3. N.CAJAL, M.IANCONESCU, I.ADERCA, E.OPRESCU,. G.DANIE- LESCU, A.BIRCA. Comparative investigations on the incidence of antipoljomyelitic antibodies in vaccinated and non-vaccinated children in the Rumanian People'sRepublic St.cercvizxframicro- biol. 196o, 11, 4, 549.. A comparative study in human embryo cell cultures of the incidence of polio-type neutralizing antibodies in 371 children aged 4 months to 7 years, before or after anti- poliovaccination with the inactivated vaccine, showed no factual differences between the two groups. Moreover, investigations on antipolio antibodies in 45 children of a closed community, carried out 6 months after vaccination showed that in spite of the vaccination, 33 (73 per cent) of the children were trinegative and only 12.(27 per cent) presented antibodies for one or two polio- virus antigen types (13 per cent for types I and II, 6 per cent for type III), In view of these results, the authors recommend substitution of the inactivated Salk vaccine by a vaccine prepared with live, modified viruses. 2o4. G.MARINESCU, P.ATHANASIU, L.PREDESCU, C.TAINDEL, C.$0- CIRNFA, Lesions of the central nervous system in patients with the res iratory form of poliomye- litis, maintained for a loin time in ies,iratory apparatus, Report read at the Institute of In- framicrobiology, November 2o, 1961. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 124 The clinical evo.Lution of two patients suffering from a severe respiratory form of poliomyelitis, who were maintained for about 6 weeks in a respiratory apparatus, with- out retrocession of the secondary paralyses, is described. Histopathologic examination showed massive dege- nerative and older medullar vascular lesions, belonging to the polio infection and more recent abiotrophic processes of cerebral malac ia,patognomonie- states of "exceeded coma" a-. gainst a background of older discrete inflammatory lesions. 2o5. G.MARINESCU. Recent advances in the specific control of polio- yelitis. Spitalul, 1961, 2,110.. The latest data concerning the sero- and vaccino- prophylaxis of poliomyelitis are presented. Improvement in the preparation of attenuates vaccines and especially the remarkable results obtained with the live attenuated polio- virus vaccine are emphasized. 2o6. M.VOICULESCU, G.MARINESCU, I.PREDESCU, P.RADULESCU, A. RADULESCU. Some poliomyelitis aspects in per- sons immunized with the inactivated antipolio vaccine. Spitalul.1961, 2, 167. Clinical study of 38 previously vaccinated polin cases showed varied clinical forms, without any difference with the non-vaccinated polio cases; overall. mortality was lo per cent in the author's observations and 7 per cent for the entire country. No marked differences were observed between the cases inoculated, with the Lepine vaccine (29 cases) and Salk vaccine (9 cases), and neither between the completely and in- completely vaccinated individuals. No polio case was reported among the children who had received the booster dose. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 125 As a rule, the clinical evolution was more favou- rable in vaccinated children, the period in which retrogression of the paralysis occured was shorter and no indelible sequelae developed. In more than 2/3 of the cases, favouring factors were observed: injections in the paralyzed member (9 children), weakening and fatiguing diseases (13 children), other fac- tors (4 children). 2o7. G.MARINESCU. On the problem of extraneuraxial lesions in poliomyelitis infections. Stud.cerc.infra- microbiol., 1961, 12, supl.,223. A description is given of various lesions, such as interstitial myocarditis (46.8 per cent), interstitial pneu- monitis (3o per cent), interstitial hepatitis C7o per cent) and glomerulo-tubulo-nephritis (47.8 per cent), occuring in the course of severe forms of poliomyelitis which resulted in death, The direct action of the poliovirus within the pattern of viraemia and of its viscerotropism, as well as the existence of factors related to the responsiveness of the body, the type of disease and its course, age, additional infectious elements and organ correlations, lie at the basis of the mechanism resulting in the formation of these visceral lesions. The presence of such lesions in severe forms of the disease may aggravate the prognosis "quo ad vitam" of poliomyelitis and should, therefore, be well-known, detected as early as possible and treated in time. 2o8. I.FRIEDMAN, S.DUMITRESCU, I.SAMUEL, T.SANDULESCU. Coxsa- ckie virus strains isolated from cases of Para- - lytic,_2oiiomyelitis in Jt3ueh St. cerc . infra- ea car e i A~r microbi.ol.,microbiol,,parazitol?,1955,6, 1-2, 51. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 126 In october 1954, ;wo strains of Coxsackie-virus were isolated, by inoculation to new-born mice of f'e-cal suspensions taken from 12 patients hospitalized in the cli- nic of Contagious diseases Bucharest. The patients from which these strains were iso- la ted, exhibited clinical signs of paralytic poliomyelitis. The anatomo-pathologic examination showed that the two strains of Coxsackie virus belonged to the G.Dalldorf type. 2o9. B.DAN, I.FRIEDMANN. The Coxsackie virus isolated from patients with paralytic poliomyelitis. Etiologic relations, St.cerc.inframicrobiol.,microbiol., parazitol., 1956, , 1-2, 51. The connexion between the Coxsackie virus and various neurologic syndromes, including paralytic, poliomye- litis type syndromes, is discussed. A description is given of two clinical cases (iso- lated peripheral facial paralysis and flabby tetraparesis with affection of the Vlth pair) in which the Coxsackie virus was found in the stools. The seroneutralization test yielded high antiCoxsackie antibody titres. The apparent epidemiologic and experimental antago- nism between the Coxsackie virus and the poliomyelitis virus, as well as the relative scarcity in the Rumanian People's Re- public of Coxsackie virus infections - a virus whose ubiquity is well known - represent the arguments lending support to the Coxsackie etiology of the paralytic syndrome, of poliomyelitis type, described in this paper. 21o. R.PORTOCALA. Electrophoretic proteinogram of the muscle of new-born mice. Changes occuring in the course of infection with the Coxsackie virus'. Riv.dell' Inst.Sieroter.Italiano, 1958, a,2, 107. Comuni carile Acad.R.P.R., 1957, 7, 12, 1o85. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 ;27 A comparative electrophoretic study of the pro- tein fractions extracted from the skeletal muscles of new born mice and the same proteins extracted from new-born mice infected with the Coxsackie virus was carried out. This study made it possible to establish the exis- tence of three protein fractions, different as regards their migration speed and temporarily called "?a", "b" and "c'-# In the course of experimental infection with the Coxsackie virus, an increase was observed in the proportion of the "b" fraction, parallel to a decrease in the proportion of the "c" fraction, 211. J.SAMUEL, N.DRAGANESCU? S..DUMITRESCU. -Research-work on the Coxsackie v lses. Giorr,Malattie Infettive e Parassit. Ed.Minerva Medica, 1957, ..Ten strains of Coxsackie virus were isolated by inoculation to new-born mice of feces suspensions from pa- tients with various affections; paralytic poliomyelitis, a- septic meningitis, myalgia, herpangina, influenza,, etc. From the anatomo-pathologic view-point, one of the strains presented.a longer period of incubation than the others, causing histologic modifications of the nervous system; the others did not produce any massive alterations of the muscular tissue. The authors try to establish etiologic relations between the presence of the Coxsackie virus in the stools of the patients and certain clinical manifestations, such as para- lytic poliomyelitis, aseptic meningitis and herpangina. 212. S.MATEESCU, N.CAJAL, Y,COPELOVICI, R.CAPRARU. Apparent clinical infection with e_.Qoxsacki-evirus ru) in'the adult white mouse irradiated with X r .s St.cert.inframicrobiol..,microbiol.,parazitoi:,1958, 9,4, 463. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 128 The effect of u adiation with X rays on the sensitivity of the 3flult white mouse to the pathogenic ac- tion of the Co: - &; k:ie virus group A is studied. The animals were irradiated with a Therapix apparatus with a 24/24 cm radiation field, at a distance of 4o cm., during 3o minutes, each animal receiving during a single exposure a total dose of 6oo r. (180 KV, lo mA, o.5 mm Cu filter). After irradiation, the animals were inoculated by intracerebral or intramuscular route with a 20o LDS, of Cox- sackie virus suspension (A2). The same virus dilution was inoculated at the same time to non-irradiated adult animals. The results showed that 66% of the irradiated ani- mals inoculated by cerebral route, contracted the lethal para- lytic disease. The biologic tests showed that the etiologic agent of this paralytic affection was the Coxsackie virus. A study of the virus in several organs and tissues (brain, pan- creas, muscles, etc.) showed that the virus persists longer in the irradiated than in the non-irradiated animals. Titra- tion of the virus likewise showed a more intense multiplica- tion of the virus in the tissue of rats submitted to irradia- tion. The results of the experiment raise the problem of the possibility of carrying out experiments with the Cox- sackie virus on irradiated adult mice. 213. I.SAMUEL, N.DRAGANESCU, S.DUMITRESCU. The presence of the Coxsackie virus in clinaA. effactions with" di.f=" ~?~r~r~riirr ~i irrrife "rant ` charactara: " St: care: inf rniagabiOl; ;inicro- biol.,parazitol:, 1958, 9,2, 193? Ten Coxsackie virus strains were isolated from the feces of 186 patients, hospitalized in the Clinic of conta- gious diseases in Bucharebt #during the November 1954 - June 1956 period, with a diagnostic of poliomyelitis, meningitis, herpangina, influenza and 4 poliomyelitis contacts. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 -129 Sepa7ration and identification of the infectious tcsv was- riot possible with two of these strains. 0?? the 8 other strains, 2 were isolated.from 2 children with benign paralytic ;poliomyelitis; . one strain was isolated from a child that-had been in, contact with- ,a polio- vqelitis case during the 1957 summer epidemic; 3 strains, were isolated from 2-children and one adult with a clinical diag- nosis of aseptic meningitis or meningo-encephalitis and, fi nally, 2 strains from 2 children with a clinical diagnosis of herpangina. From the anatomo-pathologic view-point, only one of the lo strains isolated produced minimal lesions of the muscular tissue and discrete modifications in the central nervous system-. ' ".The -other 9 strains gave rise to massive le- sions in the muscular tissue, without alterations of the cen- tral nervous system. 214. N.CAJAL, S.MATEESCU, Y.COPELOVICI, E,PACURARU. Trans- mission. of the Coxsackie virus A2) to adult White, !nice treated with-- :- Comunioariie-- Acad.R.P.R. 1959; x,'1,-81: Wishing to establish if internal. irradiation with beta rays can influence the sensitivity of the adult mouse to the pathogenic action of the Coxsackie virus (A2), strain which is not normally pathogenic to the adult mouse.-the au- th.ors inoculated by intracere'bral route 20o LD50 of a Cox- sackie virus suspension (A2) to adult white nice, which had been previously injected with an isotonic. solution of P32 (Na2HPO4), in a dose calculated for an activity of 50 C? The experiments showed that intern, irradiation with the beta rays emitted by radioactive phosphorus (P322) sensitizes. . the adult white mouse to the pathogenic action of the Coxsackie virus (A2), Vitus which is usually pathogenic only for the. new-born animal, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 13o After intraper_.t deal introduction of a radio- active stlution of Na2HP04 (P32), inoculation by cerebral route of the Coxsackie virus (A2) gave rise-in 81.8% of the animals to:a paralytic lethal disease, with prolonged persis- tence.of the virus in certain organs: and tissues'(brain, pan- creas, spleen,.:'-kidneys, muscles) 'as compared to controls. These results are due, in the opinion of some authors, to a modification of the resistance of the animal organism under the action of beta rays and to a more intense multiplication of the Coxsackie virus in the body of animals treated with P32. 215. N.CAJAL, S.MATEESCU, X.COPELOVICI. Sensitization. of adult white mice to the pathogenic action of the Co "sackie-Viras" fo1loWing- foni2ifag^ irradiation (RoeStgeh anQ"beta Fays:)"Acts"~tiroiogica"1959, 'The effect of radiations (Roentgen and.beta rays) on the sensitivity of 14-18 gr adult white.mice to, the patho- genic action of the Coxsackie virus, type 2 group A, inocu- lated after irradiation, is studied. Irradiation was applied externally and internally. For external irradiation (Roentgen).'a boo r dose was applied, employing a Therapix apparatus, 18o kv, lo mA, 0.5 mm Cu fil- ter, at 4o cm. distance, 24 x 24 cm. localizer, during 13 minutes. Internal irradiation (beta rays) was done with Na2HP04 by intraperitoneal injection of P32, with an activity of 5o-/uC. The conclusion. is reached that external irra- diation with'Roentgen-rays or internal irradiation with radioactive phosphorus injected intraperitoneally sensitizes the adult white mouse to the pathogenic-action of the Cox- sackie A2 virus, inoculated intracerebrally. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 131 Paralysis and. the lethal disease appeared in 82% of the mice in the case of irradiation with rays (P32) and in 66% of the cases with Roentgen irradiation. The mortality rate in controls ranged between o and o,8%. The virus was found in the organs and tissues of the irradiated animals for a much longer period than in non- irradiated animals. 216. R.PORTOCALA. Electron images of Mofibril_ new-born mice, in the' course"of infedtioiiwith- the-Coxsa ckie-virus. Riv.dell'Ifist:Sieroter .Italiano-1959, 34, ' 4, .263. The changes that appear in the myofibrils of new-born mice in the course of. infection with the Coxsackie virus (group A) are studied in the electron microscope. The protrusion of aatomye n at the level of the Z striae, with disappearance of the N striae and homogenization of the Q segment, followed-by hyaline degenerescence in the advanced phases of the disease are successively described; mitochondria were the only formations revealed in the myofibrils during this interval of time. An exaggerated fragility of the myo.:.._.:. fibrils, abundence of collagenous tissue and, in the pre-coma phase, the appearance of regeneration myofibrils was likewise observed. ECHO 217. M.GRUTA, Y.COPELOVICI, I.POPESCU. Isolation of an ECHO 12 virus from a meningitis patient with-exanthema. Isolation, for the first time in the country, of ECHO 12 virus, from a child with moderate meningeal syndrome, is reported. The virus was isolated in trypsinized human embryo Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 132 cell cultures. The presence o.,: ECHO 12 virus is reported for the first time in serous meningitis. 218. G.MARINESCU, LIU CIUN PAE. Lesions induced by ECHO virus (tpe 9) in cortisone treated adult and new=born white mice. Stud.cerc.inframicrobiol. 196o, 11, 3, 4o9. Using ECHO 9, carried through numerous passages on monl.ay kidney cell and human embryo call eultures,a soserate myo- sitis process was. obtained in the new-born mouse inoculated by intramuscular route and a moderate hepatitis process in the new-born and adult mouse, inoculated by intraperitoneal route.. . Association of cortisone, 3 days and 1 day before inoculation of ECHO virus, resulted in diminution: of the inflammatory and degenerative processes, due to its antiedema- tous and antiinflammatory effect. A comparative study should be done with freshly isolated strains, in order to establish if their pathogenicity is modified, and if their cytopathic effect may serve for the diagnosis of ECHO infections. 219. M.GRUTA, Y.COPELOVICI, V.ARMASU, C.LECCA,-V.VASILESCU. Investigations of the incidence of hemagglutina- tion-ihhibition-'antibodiesto-certain ECHO virus strains"in-some-eommnnittes-in-Bacharest:Stud.cerc. inframicrobioi: -i96o; "Ui 4; - 79; A study was carried on anti-ECHO hemagglutinating- inhibiting antibodies in the sera of 385 healthy individuals from two industrial and a military unit in Bucharest. Anti- ECHO 7 antibodies were found in 63.3 per cent of the persons investigated, anti-ECHO'9 antibodies in 36.7 per cent and anti-ECHO 19 antibodies in 31 per cent. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 133 A parallelism was seen to exist between the inci- dence of anti-ECHO 9 and 19 antibodies, however With differ- ence in the titers. 22o.. M.GRUTA, Y.COPELOVICI,.V.CIOBANESCU, MOGROZA, M.DAMIAN. Comparative study of complement fixing and h m agglutinotiot~-iahibitink'b tibbdies to-ECHO ?11 and ECHO 19 viruses in a" childi*h1 b d62M h1tt: Stud.cerc.inframicrobiol.,1961,'12, suppl.,2o3; Rev.Sci.Med...1961, 6,.1-2, 53. In a,closed children's community, hemagglutination- ir_hibiting and complement` fixing antibodies to ECHO 11 and ECHO 19 viruses were found to be totally absent in the o-6 months age-group.'.Low-titre antibodies to ECHO 11 antigen (1/32 - 1/64) and higher titres to'ECHO.19 antigen were de- monstrable in the-hemagglutination-inhibition test. The almost similar results of both tests determine the authors to suggest the hypothesis that within the ECHO virus group the complement fixing test may be considered as type-specific. 221.. G?.MARINESCU,,.G.SORODOC, D.SARATE,pNU, S.DUMITRESCU. A st dv on th`gglutinatingProperties of cer- tain ECHO":~iiiis strsibs"ystsufi'$4lflafl"af`ytfb@ytes" of different grbttbs~8hd the ex`vtFociites"sf'sb~ne" animal .spec ie'Stud,cerc:iriframidt6bidl:"1961, 12, suppl, 2151 Rev.Sci.Mgd. 6, 1-2, 61. A description is given of the agglutination reac- tion of type 11, 12, 19 ECHO virus strains with human red cells belonging to various: groups of the RH-and ANO-systems. As a rule, no titre' differences were recorded. Obviously the reaction is dependent on the cell receptors involved in the Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 134 enzymatic process occuring at the level of the red cell and has no relation with the specific..blood,.group.antigens. The agglutination reaction of some ECHO virus strains'wich strongly 'agglutinated human erytrocytes, yiel- ded weakly positive and inconsistent results and low titres (1/l0 - 1/20) with the red cells of puppies, rabbits, hamsters and mice. Two of the 6 ECHO virus strains yielded moderate agglutinating titres,(1/4o - 1/80) with monkey-'red cells. This raises. the problem of. the heterogeneity;sand'possible correla- t~ on . of human orphan, viruses with animal strains. 222. G.MARINESQU, P.ATHANASIU, O.MITROIU. Interstitial pneumonia and experimental encephalitis-ih-hew- born white' mice;`3.noculated'with"the-hemagglutina- tingECHO ll virus. Report-read at-the Ihstitute' of Inframidtbbiology. October 16, 1961. Inoculation of the hemagglutinating ECHO 11 strain into the cervico-dorsal lipo-Muscular mass, induces in new-born white mice encephalitis and interstitial pneumonia lesions. From the lung and brain of the mice sacrificed l and 3 weeks after inoculation, the virus was-isolated by inoculation into monkey kidney cell cultures. 2239 M.K.VOROSHILOVA, G.MARINESCU, V.I.JEVANDROVA, M.S.BALAIAN. Investigations on. the hemagglutination of some ECHO virus strains" isolated ifl"-the' USSR dui-if g antipoliomyelitis: vaccifiatiozi.: Report read" at" the Institute of lhfp picrobiology, July 4, 1960. Hemagglutination with human group 0 erytrocytes of 16 ECHO virus strains (types 8, 11, 12 and 19) isolated in-the USSR from the feces of children immunized with the live attenuated Sabin polio-virus vaccine, was intensely positive for 6 virus straths (two ECHO 11 strains, three ECHO 12 straits and one ECHO 19 strain.) Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 135 No-definite parallelism was observed between the hemagglutina:ting.and infective potential of these strains. Hemagglut.nation-inhibition with type hyperimmune sera yielded much higher titers than in the controls; inhi- bition of cross. hemagglutination gave very low titer:st which confirms the specificity of the test and the possibility of using it for. diagnosticI purposes. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 VI NEUROVIRAL DISEASES 224 - 339 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 136 224. R.PORTOCALA, N.CAJAI,. Histopatho1ogic alterations of the retina neurons"following"experimental~i~t=" fection- with- the . herpes-virus '- Bul.Stiint. " Acad.R.P.R., Sec tt St.Med. -1.949, 1, 5, 411. Two strains of herpes virus, a neurotropic and a dermotropic strain, were inoculated to rabbits on the sca- rified cornea or by intracerebral route. The cellular le- ~sions, produced by the herpes virus at the level of the re- tina, were studied. The nuclei of the. neurons that constitute the chain of ganglionic cells are the site of alterations cha-. racteristic of the herpes virus when it is inoculated intra- cerebrally. The virus propagates by septinearitla route along the optic nerves, reaching the retina, where it localizes in the nuclei of the ganglionic cells; here it multiplies and produces veritable colonies and typical inclusions. The lon- ger is the incubation period of the experimental disease.,. the greater is the number of neurons affected. Inoculated on the scarified cornea, the herpes virus did not give rise to any histopathologic alteration in the retina. The route taken by the germs from the inoculated cornea to the'retina being longer in this case, it explains why the rabbits infected by the corneal route do not present herpes alterations of the retina. In the light of the data of this paper, it is assumed that "cortical blindness" of experimentally infected rabbits is due to a great extent to the alterations induced by the herpes virus in the retina and that certain distur- bances encountered in human ocular pathology, whose etiology is not well established, could be explained by localization of the herpes virus in the retina and induction of certain lesions in the ganglionic neurons. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 137 225. ACAD.ST.S.NICOLAU, G.BALMUS, N.CAJAL. Transformation of the herpes recidivans virus into' herpes _ simplei virus bt'adaptatiOfl tb-the-tabbit"brsin; ------ c:inframic20bi01:; 1950;'i;~ 2;-137. The herpes virus, collected from the vesicles of 2 herpes recidivans patients, was inoculated by swabbing on the scarified skin of 4 persons. After 3-4 days, herpes with a relapsing character appeared in 2 of these persons. On the other hand, the initial virus was also inoculated to a group of rabbits on the scarified cornea, causing a keratitis fol- lowed by typical encephalitis. A number of 5 serial passages were performed on the brain of a rabbit, resulting in a short- er period of incubation, as well as increased aggressivity of the virus. Brain fragments from the last passage were inocu- lated to 5 persons by swabbing of the scarified skin. Inocu- lation was positive in 3 of the 5 cases and no relapse of the herpes eruption was observed in these patients, which were followed up .during 8 months. The induced herpes presentee: the symptomatology of herpes simplex. Therefore, by adaptation to the central nervous system of the rabbit, the herpes recidi- vans strain behaves like.an ordinary herpes simplex strain. 226 N.CAJAL, R.DINU, P.STROESCU. The herpes virus and catarAct in rabbi.tsw St:cerc:inframicrobio1., mietoblo1 pi Ztt01.; 1951, 2, 1, 163. The authors describe a case of cataract in a normal rabbit'which had stayed several days in the same hutch with a rabbit inoculated by corneal route with the herpes virus, closely resembling-the cases reported by Nicolau and Koprowska in 1931. About a month after having being put together with the rabbit inoculated with the herpes virus, the non-inoculated animal presented a mature cataract of the right crystallin and an incipient, posterior polar cataract in the left crystallin. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 138 In order to prove that the rabbit had contracted a subclinical herpes following contagion in the hutch and had then become immunized, it was inoculated with the herpes virus on the left cornea. Twelve days after inoculation, when the control animals died of herpes kerato-encephalitis, the rabbit with cataract, which had survived, was sacrified and the brain examined histopathologically. On the sections, stained by the Nissl, Spielmeyer and Mann 6-aLod,as he picture of an old herpes meningoencephalitis was found in the left hemisphere together with an area of softeninr7 in the occipito-calcarine cortex and in the axis of Ammon's horn; in the right hemisphere dis- appearance of the pyramidal cells in certain spots and of some cells in Ammon's horn could be observed: at the level of the entorhinal one of the pyriform lobe, herpetic inclusions were noted. Both the. mature and the incipient cataract may be the result of tropl disturbances caused by the lesions described, a fact which might justify an eventual cortical representation of the trophic disturbances of the crystallin? 227? ACAD.A..KREINDLER, N.CAJAL, I.OLTEANU? The action of tellurium in intracerebral iniectl.Of iii"' rrXp~- rimei~tal " he5rbe~ ~ etace~ihalY.'~is ~ of "' the ~ raT~b~.t ?" Bu.1?Stiii ~oAcad""R?PoR~ Sep :St~Med:;~I951;'2, 1, 329. In view of establishing the way in which experi- mental herpes encephalitis in the rabbit is in-joluenced.by the parallel evolution of a toxic encephalitis of the tellu- r;Wa.encephalitic type, a study was carried out on the effect of an intracerebral injection of black metallic tellurium, at the height of a herpes neuraxon infection. The experiments showed that injections of a 1% black metallic tellurium suspension in small amounts (o.lo ml) into the brain of rabbits at the height of herpes neuraxon Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 139 infection prolongs the evolution of the disease, sometimes even leading to recovery. The attempts made at tra=T_'Iing this vi- ral disease by intracerebral inoculatior,starting from the brain of these rabbits, yielded negative results, demonstrat- ing the existence of an autosterilization process. Intracerebral injection of a larger quantity of the tellurium. suspension (0.25 ml.) causes death of the animals in the following 24 to 36 hours. Intracerebral passages of brain emulsions from these rabbits constantly yielded positive results. From the histopathologic view-point, a large num- ber of characteristic inclusions were found in the first group ..1. animals. 228. R.PORTOCALA, N.CAJAL, E.LISSIEVICI. $yperglycemia followr_.' ing experimental herpes encephaliti8-1f the' .xabbit. Comu.nic9rile"'Aoad-.R;P;R:-1951;-1;-507: ------------ Experimental herpes encephalitis in the rabbit, subsequent to sub-dural inoculation, is followed by a sig- nificant increase of glycemia. This increase begins on the second day after inoculation, attaining maximum figures on the in which the animal dies. Saline solution and normal brain suspension do not modify the glycemia curve. Glycosuria is absent in the animals with herpes.. 229. N.CAJAL, E.LISSIEVICI. The evolution of glycemia in ex- perimental herpes infection _ in- tie-Iabbitm - '- -' ? . St o cerc ~ i::~"~ amicrobiol m .uiicrobiol: ~, parazitol. , 1952, 3, 3-?4, 161. The glycemia curve in a group of 15 rabbits ino- culated on the cornea with the herpes virus is studied. In 9 of the animals an increase of this curve was observed, running parallel to the appearance of the characteristic keratitis and Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 encephalitis. The glycem a ,:avve continued to rise until death of the animals occured. Similar results were obtained in another group of 9 rabbits, inoculated by intracerebral.route with the herpes virus, the symptoms of herpes encephalitis developing parallel to an increase of the glycemia curve. A comparison is made between the hyperglycemia obtained under experimental conditions and human diabetes and the viral etiology of diabetes, which could be caused either by the herpes virus or by another virus capable of modifying sugar metabolism, is assumed. 23o. N, CAJAL, R.DINU, S?MATEESCU, G.POPESCU. Evolution of experimental herpes neuroinfection in rabbits . with` a'-xnodifi ed ` reactiVit ?of vous system,: '. ComubicariIa"'Aead;ReP R: "1953; i., 9=1.0, ~341. The comparative, study of the evolution of expe- rimental herpes in rabbits, with a modified reactivity of the central nervous system, showed that the incubation period is much shorter in the animals submitted to repeated electro- shocks and slightly shorter in the animals in which the reac- tivity of the central nervous system was inhibited by chloral hydrate. The results obtained showed the importance of the cerebral cortex in the defense of the organism against inframicrobial agents. 231. ACAD.ST.S.NICOLAU, GvIVIARINESCU. On the possibility of paraspecific prophylaxis by means`tf"exberiffiaetal herpatizatio~~al~.o~i,~a1i'Cis: "'Re~iart"1~ead."at~"" the Fourth" anti=polio!i7elitIs"'International Sym- posium, Bologna, September 1956. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 141 Previous researches showed that the reaction of the organism, spontaneously infected with the poliomyelitis virus, against experimental herpes inoculation, definitely indicated a progressive attenuation of the usual sensitivity to the inoculated virus (duration and aspect of the eruption). This attenuation which begins in the second month after the onset of poliomyelitis, is later transformed into a strong resistance. Based on the assumption of the possibility of developing a pa.?-~ _i:iwie refractory state against poliomyelitis, following herpes immunization, herpetization of persons liable to contamination is suggested. This paraspecific prophylaxis of Heine-Medin's:. ;P bd performed at the same time as the specific vaccination. 232. N.CAJAL, C.BABA. Action of vitamin C in experimental herpes neuro=infection;"St:cerc:infrainicrobiol., microbio1;,-paraZitei;, 1957, 8, 1, 23. Vitamin C administered by intramuscular route to rabbits inoculated with the herpes virus on the cornea, acts differently according to the law of large and small Danielopolu doses: in small doses it prolongs the life-span of experimen- tally inoculated animals, whereas in large doses it curtails the duration of the disease as compared to the controls. The mechanism of action of these differences seems to reside in the stimulation of acetyicholin and gamma- globulin production. 233. S.NICOLAU, G.BALMUS, N.CAJAL. Experimental transformation of the herpes recidivans virus into herpes simplex virus.. Giornale di Malattie Infettive'e Parassit. 1957, 9. With a view to transforming the herpes recidivans virus into a herpes simplex virus, by adaptation of the reci- Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 142 divans virus to the rabbit brain, experiments were carried out with 2 virus strains from 2 patients with arythmic herpes recidivans. The virulent vesicular fluid, collected from one of these patients, was inoculated by scarification on the skin of 4 persons, 2 of which presented positive results with attacks of herpes recidivans. The same virulent fluid was inoculated at the same time on the cornea of rabbits. The brain of one of these rabbits, which died of herpes encephalitis, formed the starting point of 6 serial cerebral passages on rabbits. The brain of the 6th passage was used for dermal inoculation to 4 persons, 2 of which presented positive re- sults: eruption of herpes simplex. Another similar experiment with vesicular fluid from another case of herpes recidivans yielded identical re- sults, demonstrating that, by repeated passages on rabbit brain, the herpes recidivans virus is transformed into a her- pes simplex virus, 234. R,PORTOCALA, N,CAJAL, E.LISSIEVICI. Hyperglycemia in experimental herpes, The National"Congress-of Med.Sciences Bucharest, SecV.Neurol.,endocri- nol . , 1957, 620 The influence of experimental herpes virus in- fection on the glycemia curve is studied in rabbits, after their inoculation by cerebral and corneal route, From the data obtained, it results that the rabbits inoculated with herpes virus exhibit an increase of glycemia, which yields maximum values in the pre-lethal period. These experiments show therefore that introduc- tion of the herpes virus into the organism of the rabbit by central or peripheral inoculation, gives rise to a locali- zation of the germs also in the glycoregulator centers, where they stimulate or even damage the' neuron with subsequent appearance of hyperglycemia. These lesions are irreversible Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 143 in the lethal cases and reversible in the non-lethal auto- sterilized cases. An analogy is drawn between the aspects obtained in experimental herpes infection in rabbits and those of cli- nical human diabetes; the possible herpes origin of this di- sease-is discussed. 235. R.PORTOCALA,.V.VELICICOV, Y.COPELOVICI. Cyto-chemical investigations in experimental herpes'( eso ribonucleic acid: `St: cef'c: iSfratfiicrobiol: ffiicro- biol.,parazitol., 1957, 8, 2, 149. The study starts from the premise that multi- plication of the herpes virus requires part of the nucleo- protein and determines stimulation of desoxyribonucleic acid synthesis. Experiments were carried out on rabbits inoculated by intracerebral route with the herpes virus. The brain sec- tions obtained were stained by the Mann and Feulgen method. During the first stages of the infection, infini- tessimal amounts of desoxyribonucleic acid are revealed within the nuclear virus colonies, As the cytologic changes become more accentuated, nucleic acid accumulates in the virus colony to the detriment of the remaining ehromatin. The amount of desoxyribonucleic acid increases within the inclusions that appear in the midst of the virus colony. This may be due, on the one hand, to impairment of normal cellular metabolism, that tends towards a stimulation of nucleic acid synthesis and, on the other hand, to uptake of residual chromatin substances du- ring inclusiogenesis. The results obtained make it possible to establish an analogy between the mechanism of stimulation of desoxyribonucleic acid synthesis in the course of the phe- nomenon of bacteriophagia and the phenomenon determined by pe-- netration and multiplication of the herpes virus in the neurons of the experimentally infected rabbit. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 236, R.PORTOCALA, V.BOERU, R.CAPRARU. Investigations on hyperglycemia in experimental"herpes:(Ladaded by er- and by oglyce~iaa St?cercaiafraffiicrs= bic14smi6rbbio1m;JaAa2it6l., 1957, 8,3, 341.0 A study was carried out on the evolution of induced hyper- and hypoglycemia in the course of experimen- tal hyperglycemia in rabbits, caused by intracerebral ino- culation of the herpes virus. Induced hyperglycemia was obtained by oral administration of glucose, 'hypogglycemia by subcutaneous injection of insulin. Evolution of glycemia was studied with the aid of the Hagedorn-Jensen microreac- tion. Under these conditions, the curves obtained in the animals which showed an accentuated hyperglycemia following herpes infection differed from the curves obtained in the control animals. The reactivity of the experimentally in- fected animals differs to a greater extent from that of the control animals, the more accentuated is the herpes glycemia. The aspect of the curves pleads in favour of an approach between the response of the herpes-inoculated rabbit and that of persons with aoglycemia, submitted to the tests of induced hyper- and hypoglycemia. 237. I.SAMUEL. Electrophoretic modifications in the sera of rabbits experimentally infected with the herpes virus. Comunicarile Acad-.R.P.R. Sect*iSt..Med;- 1958s 8, 11, 12210 The results obtained in electrophoresis of the sera of 14 rabbits experimentally inoculated with the herpes virus, the Bruxelles strain (5 on the scarified cornea and 9 by cerebral route) are reported. The electrophoretic determinations were done with an IK?3o apparatus. The curves were interpreted accord- ing to the method of P.Cazal and G.Carli, obtaining gradients of the Tisseli .q type. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 145 In the animals inoculated on the scarified cornea, a decrease is observed in the albumin-globulin ratio, reflected by a marked increase of betaglobulins and a constant decrease of albumin, together with slight modifications in the proportion of alpha and gamma globu- lins, which as a rule remain within initial limits. In the animals inoculated by cerebral route, similar modifications are observed, but of lower intensity. In this group, the gamma globulins have at the beginning a marked tendency to decrease and then begin to increase slightly. 238. I.ADERCA, M.IANCONESCU. Researches on the herpes virus lished in in human amniotic ahd emabryo tissue"cfi~tures: Report-read-at-the-Sed6ad-Cotgro8a-af"Hiingarian Microbiologists, Budapest, Sept.22-26, 1959- A brief summary of the data obtained and pub- our country in previous works on the herpes virus in cell cultures,. 239. M.CEPLEANU, N.CAJAL. Research on the transmission of herpes to"the goiden-hamster: (Crieetuss auratus.) St core: ififrafnicrobi1 a,' 1959, to 3; 349 In two experiments carried out on 51 hamsters and 8 rabbits, with 2 strains of herpes virus (P 5433 and Bruxelles), the golden hamster was demonstrated to be an animal species sensitive to experimental infection with the herpes virus. The cerebral and intranasal routes are very sensitive, whereas cutaneous, subcutaneous, intramuscular and intraperitoneal inoculation are ineffective. Experimental herpes infection of the hamster is reflected morpho-pathologically by a typical meningo-ence- Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 146 phalitin, constantly accompanied in nasal inoculations by bronchompneumonia of the interstitial type. 24o. G.DANESCU-POPESCU, N,CAJAL. Evolution of experimental herpes in cortisone treated rabbits:"St.cerc. inframicrobiol;, 1959, io;1, 71; - Experimentation with strain 5433 of the herpes virus on cortisone treated rabbits at various moments, showed that : experimental herpes infection in this animal species is greatly aggravated under the action of cortisone acetate. The influence of the suprarenal hormone is more- strongly revealed when the animal has been infected by cor- neal route. The microscopic examination showed in the cor- tisone treated animals, in comparison with the controls, di- minution of the infiltrative reaction, an accentuation of the congestive reaction and a marked development of virus colonies, with decrease in the number of characteristic in- clusions. 241. MOIANCONESCU, I,ADERCA. The herpes virus in cell cul- tures, III.- Cellular" lesions" firod0.ced.'br the herpes ~rirus ' 0f~"hCii~afi' embPyo" afifl" aiotic"cell cultures. " " St. care: iflframicrobiol o ; ' 1959; '10 ; 4, 487. The cytopathogenic effect produced by the her- pes virus in human embryo and amniotic cell cultures, pre- sents aspects similar to those of the lesions produced by this virus in the organism, The aspect of the cytopathogenic effect is charac- teristic enough to permit a swift and presumptive diagnosis of herpes virus, to be confirmed by seroneutralization. Dissemination of the herpes virus takes place both directly from cell to cell and by spread through the culture medium. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 147 242. R.PORTOCALA, V.VELICICOV, Y.COPELOVIC.I. On the lution of intranuclear desoribonucLeic a=;,',... in `the"coiirse`of experimental herpes-of the brain ste~i Rev': Sci Med: 1959; -4.9 -100;---- Experimentation was started from the premise_ that multiplication of the herpes virus may be assumed to require part of the nucleoprotein in order to determine sti- mulation desoxyribonucleic acid synthesis. Investigations were carried out in rabbits inoculated by intracerebral route with herpes virus. The encephalon sections obtained were stained.by.the Mann and Feulgen'method. In the first stages of infection, infinitessima]_ amounts of desoxyribonucle'ic acid were found in the nuclear virus colonies..-As=.the cytologic alterations became more accentuated, nucleic acid accumulates in the viral colonies to the detriment of chromatin remains. Desoxyribonucleic acid amounts increase within the inclusions that 'appear in the virus colonies. This may be due, on the one hand;, to alteration of normal cellular metabolism, which tends to wards stimulation of nucleic acid synthesis, and on the other, to a taking up of chromatin residues in the course of inclusion genesis. Herpes., 243. I.ADERCA, M.3ANCONESCU. Investigations on herpes virus in huzhan amniotic ahd' embryo - celi. cultures, " - Microbibl ar~2itblo; e.ide ibi?~~96o -2 155a The susceptibility of human fibroblast cultures, obtained by'trypsinization of 1 to 3 months old human embryos, was studied in view of isolating the herpes virus directly from the patient.. Six strains were isolated from 6 cases presenting typical herpes eruptions and 7 strains from patho- logic products obtained from cases of ulcerous stomatitis, non-diphtheric croup, measles and encephalomyelitis. The advantages of this method as compared to other methods for Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 148 the isolation of herpes virus are shown. Morphologic study of the herpes virus infected cells showed the presence of characteristic lesions, similar to those in the animal or human organism. These lesions and their evolution, described in detail, explain the spread of the virus in the organism and cell cultures. 244. N.CAJAL, HUNG TAO, V.IONESCU, G.MARINESCU, S.MATEESCU. Electrocardiographic alterations in rabbits with experimental herpes. Stud.cerc.inframicro- biol., 196o, 119 21 219: Electrocardiographic examination of rabbits ex- perimentally infected with herpes virus showed morpho-func- tional alterations at the level of the heart. The low intensity of the myocardial anatomo- pathologic lesions and the impossibility of isolating the herpes virus at this level, pleads for a central nervous origin of the cardiac alterations occuring in the course of experimental infection. In view of these results it is assumed that the herpes virus might play a part in the etiopathogeny of cer- tain cardiac functional disturbances encountered in the hu- man clinic. 245? I.ADERCA, M.IANCONESCU, A.BIRCA. The herpes virus in cell cultures. I.-Isolation-of-herpes-giros-in human? embryo" cell" cu.Itures: "St~iQ;cerc: i~if~ea=" microbiol?,"1960,"119 -'-2243. The sensitivity of human embryo cell cultures obtained by trypsinization of whole embryos is studied in view of isolating the herpes virus directly from patients. From the material collected from 13 cases of typical herpes eruption, 13 virus strains were isolated and identified by Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 149 seroneutralization and the production of keratitis on sca- rified cornea of a rabbit. Another 7 herpes virus strains were isolated from products obtained from ulcerous stomati- tis, non-diphtheric croup, measles and encephalcmyelitis cases. The advantages of this method over other methods for the isolation of herpes virus are discussed. 246. M.IANCONESCU. Herpes virus in cell cultures. II.- The incidence- of _ antiherpes- antibodies; studied.? - by the"serofie~ittaiiatio8~teactio is tiSaue ____ res:" Sti.d:core:infraffiier8b1oi '196o;"11, 2, 249. The incidence of antiherpes antibodies is inves- tigated by means of the seroneutralization test in cell cul- tures. It is shown that the antibodies appear early already in the second year of life and attain a proportion that ranges from 6o to 9o per cent. The value of serum antibodies in antiherpes immunity is discussed. 247. R.PORTOCALA, N.CAJAL. The presence of Negri bodies in retina neurons - i.fl' natural "rabies dine - to - street virus: Bui:Stiiiit;Acad:R:P;R;Sect:St. Med., 1949, 10,109 891. The rabies street virus in its centrifugal dis- semination, gives rise to Negri bodies in the ganglionic neurons of the retina. These formations could not be found in the corneae examined, due probably to the small amount of germs that reach, by septineuritic route, a :odium so little favourable to the development of the rabies virus. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 150 248. N.CONSTANTINESCU. New data on the prophylaxis of rabies. Rev.St,Med.9'.i950;"Ii1__8l9;' ____.__...._... ~_._._.__. General report on the problem of specific and non-specific prophylaxis of rabies in man and animals.. 249. N.CONSTANTINESCU, A.TOMA, C.DRAGOMIR. The process of Nektigenesis and alterations of. the"netzt6h_ _. in"rabies; "AL;Acad;R-OPaR;-SeeV:St;MOd.;;1950, 2g-15'345. The morphopathologic study of cellular altera- tions and the inclusiogenetic process showed that no rela- tions exist between the frequency of the Babe*-Negri bodies and the intensity of these degenerative processes in the neurons. The latter process involves not only the nucleus, but also the cytoplasm of the.neuron and its frequency seems to be conditioned by the duration of the paralytic phase of rabies. The specific nature of the cellular alterations were confirmed in the spermophil., an animal species whose neurons react by a massive inclusiogenetic process, even in rabies with the fixed virus. 250. ACAD.ST.S.NICOLAU, MIRCEA V.BABES. "The Babe*-Negri bodies" and not "the Negri bodies":"Rumanian science-is first-in the discOvery'of rabies Inclusions' Bul.Stiint.Acad;R.PoR:Sect:St:Med., 195o,2 g 9, io75- As far back as 1893, in the first edition of his "Textbook on the therapy of infectious diseases", pub- lished by Professor Penzolt in the Fisher Printing-House in Jena, Victor Babe* describes rabiesinclusions in the cyto- plas the nervous cells in the form of "small vacuoles, coat, _.r_.g a hyalin corpuscle", to years before Negri. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 151 It is thus demonstrated that Babes was the first author to give an accurate interpretation of the rabies in- clusions. It is therefore requested that these formations should in future be named the "Babel-Negri bodies" and not "Negri bodies", a designation which does not correspond to the scientific truth. 251. ACAD?ST.S.NICOLAU, N. CONSTANTINESCU, C.DRAGOMIR. ELPUIU Ax.,t ;i rabies vaccination of dogs with the fixed vi?ixs attenuated.:" by "~ tbylic alcohol' and emulsioned in mineral oils`Afi:Acad:R:P:R:"Sec .St:Med. 1950, 2;_18;"461. Although the oleate vaccine prepared with the fixed rabies virus attenuated by 5o per cent alcohol is patho- genic when inoculated by cerebral route, by peritoneal or mus- cular route it has no effect. The immunity developed is per- manent; all the vaccinated rabbits and dogs were immune to the test=infection with the street virus inoculated-byomuseuler route (all the control rabbits and 6 of the 7 control dogs inected by this route contracted rabies), but 2 of the 5 dogs and 3 of the 6 rats resisted to the cerebral inoculation, which killed all the control animals. 252. A'CAD _oL,'.ST.S.NICOLAU, N.CONSTANTINESCU,tDRAGOMIR ;E.PUIU'. Comparative study of septineuritis and "closed rabies" in vaccinated' and' nbn=Veceinated" nabid animals and'men -`Ab:Aoed;R.P;R;Sec4 St:Med:195o, 2, 20,..617:.._.. The investigations carried out on 7 human cases of rabies and 54 animals, experimentally infected with the street virus, showed that rabies septineuritismanifested by the presence of the virus in the peripheral nerves, is negative Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 1.52. in the vaccinated persons. Therefore, the street virus.is present in the encephalon, but does not spread centrifugally through the intermediacy of the nerves to contaminate the sa- livary glands. In vaccinated cases, clinically apparent rabies becomes a non-transmissible infection, the saliva of the man or animal is no more virulent, constituting therefore an ac- tual "closed" rabies. Negative septineuritis and consecutive closed rabies was revealed in 86% of the vaccinated animals and men, which however died of rabies, as compared to 2o% reported in n6n-vaccinated cases of rabies. The phenomenon is explained by tissular immunity of the peripheral nervous system, acquired by vaccination and prevention of centrifugal spread of the street virus from neuraxon to the peripheral nervous system resulting in nega- tive septineuritis and closed rabies, non-transmissible by the salivao 'P- 51 3, ' ACAD ,.ST.S.NICOtAU, N. CONSTANTINESCU, C.DRAGOMIR. Rabies with -a long incubation in vaccinated per_cns __StocercoinfranigerOb1OY:;_i9ga;~. 1-29"17. Two cases of human rabies are described, cor- rectly treated with anti-rabies vaccine in which however the disease developed after a long period of incubation, of respectively 158 and 738 days. Experimental data showed that failure: of the antirabies treatment was not due either to the existence of an antigenic variant - different from the street virus - or to a "reinforced" virus strain. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 153 254. :.: ACADy.ST.S.NICOLAU, N.CONSTANTINESCU, CONSTANTA -DRAGOMIR. Study of the h;.stologic lesions in the brain of experimental animals in )inapparent rabies infections St.cerc.inframicrobiole,195o, Experimental investigations and histo-patholo- gic examinations, made evident the existence of an "'Inappa- rent rabies infection". Although rabies symptoms are absent and the animals infected with sub-rabigenic doses survived, this inapparent infection exists even after 3-4 months, and oxyphil degenerescence lesions of the encephalic neurons can be observed, infinitely more intense than in lethal ra- bies in which the inflammatory process is predominant, due to the short evolution of the disease. The fixed or street rabies virus is in latent conflict with the neuron, determining the oxyphil degeneres- cence reaction, compatible ith the apparent state of health. The cellular alterations, observed in ~tnapparent rabies infection, explains the state of cerebral tissular im- munity discovered by Nicolau and other authors in rabies. 255. P.STROESCU. The presence of rabies inclusions in the carotid" lomfls' ifs-fah" aDd- afiima18 - that" died- of" rabies: "St: a +c: iflP~affi1cPabIOl:;i~icgOblol., parazitei., 1951, 2, 1-4, 117. The histopathologic investigations carried out on products obtained from human or animal cases which died following rabies with the street virus, showed the follow- ing in the intercarotid glomus : Rabies inclusions are revealed in the cyto- plasm of the nervous ganglionic cells, in the thickness of the nervous periglandular plexus or in the cells that constitute the sympathetic microganglia. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 - Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 154 These formations are likewise to be found in the rare ganglionic cells encountered within the inter-carotid gland and interglomerular plexus. In the glandular cells proper, the rabies virus could be detected in the form of inclusions in a single hu- man case. In the rabies infection, the inter-carotid gland cells are in a state of hyperfunction, either due to the presence of the virus, or to incitation of the ingected afferent nerves. 256. N.CAJAL, S.MATEESCU. Intranuclear Babes-Negri bodies, S t. care . inframicrObiol: ; ~iie obioi ; paDa itoi: , 1952, 3, 3-4, 200. Experimentation with a street rabies virus strain with long incubation (21 - 23 days by intracerebral route and 47 - 53 days by muscular route), showed in 3 rabbits, besides numerous cytoplasmic inclusions, the presence of few inclusions situated in the nucleus of certain neurons and of certain glial cells. There were one, two or three inclusions in one nucleus, measuring approximately 1-3 /h in-size, with a more or less homogenous aspect and surrounded by 'a charac- teristic halo. The nuclear inclusions, stained by the Mann method, appeared - similar to the cytoplasmic inclusions - in various shades from blue to red. On the basis of these observations, it is con":.. eluded that rabies inclusions can no more be classed in the "cyto-oykon" group, but in the "cyto-kario-oykon" group, cytoplasmic inclusions being prevalent. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 155 257. ACAD.ST.S.NICOLAU, N.M.CONSTANTINESCU, A.TOMA, I.ADERCA, E.DUCA, M.DUCA. Experimental rabies infection with the "neurptropic""fixefl?virts. St:ce~c: inframicrobiol:;~icrobiol:;parazitol.,1952, 3, 348 k7. Rabigenous activity following peripheral ino- culation of a fixed rabies virus strain, modified by adapta- tion to the brain of the ground squirrel (Citellus citellus) and of the rat is studied. This strain is extremely virulent and becomes exceptionally aggressive, causing rabies paralysis in 2o per cent of the rabbits and 42 per cent of the guinea pigs inoculated by subcutaneous route; in loo per cent of the rats, 75 per cent of the rabbits and 37 per dent of the dogs inoculated by intraperitoneal route; in loo per cent of the rats, 5o-6o per cent of the rabbits inoculated by intra- muscular route and finally 7o per cent of the guinea pigs and 57 per cent of the rats inoculated by intradermal route. 258. P.STROESCU. Researches on.the "Babe*-Koch" formations in street-rabies:''St:cerc.inframicrobiol.,mi- crobiOl.,parazitol., 1952, 3, 1-2, 89. Experimental and histologic investigations have shown that the granular formations described by Babes in the nervous cells of animals that died of street rabies, repre- sent a histologic reality. They appear under 3 aspects : pulverulent formations, close to the size of the virus, dis- covered by Babes and later by Remlinger by means of the ultra- filtration method; slightly acidophil corpuscular formations containing 1-5 small granulations, more resistant to argentic decoloration than the corpuscular mass and formations with arborescent aspects, which are extremely argentophil. The granular and corpuscular formations cannot be identified with the live virus itself, but represent evolutive stages in the formation of the Babep-Negri bodies. These formations are Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 l56 numerous in the ,;ells c the animals infected with street virus -r. or a~, . r another species; their frequency of adaptation on the same species. ` he se a rn a .ions represent the morphologic expression =->. th3 degree of cellular resistance to the ac- tion of the rabies virus. 259- N.CAJAL, S.MATEESCU, EL.LISSIEZICI, M.CEPLEANU. The evolution of rabies due to the street rabies hires, in-rabbits- 8ubnitted' tai-the - action-.of certain isolated"'or associated stimulents." (heat - milk). St.cerceinframicrobiol:,micro- biol-Dparazitol., 1953, 4, 1-2, 79. Investigations were carried out in 26 rabbits on the reaction of the organism to the street rabies virus after an injection with milk or after thermal stimulation of the skin, as well as after association of the two sti- mulents The results of the experiments showed changes in the period of incubation. As compared to the controls who died after an average period of 14 days, the group in which stimulents were applied separately died after a period of 21-23 days and in the group in which the two stimulents were associated (thermal stimulation + milk injection),, this period was of 47 dayse The authors conclude that when the two stimu- lents are applied separately, the response of the cortex is manifested by an increase in the defence reaction, which does not exceed however a given threshold. When the two stimulents are applied simultaneous- ly, according to the technique of elaboration of conditioned reflexes, the defence reflex is much stronger. This is due on the one hand to summation of the stimulations' and defence responses of the cortex and, on the other hand, to the mecha- nism of negative induction of the previously fixed dynamic stereotype- Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 p {~ n An ern 157 -'Co. R.DINU, CGIACOB, E.NASTAC. Resistance to rabies in rats with experimental reflex"epilepsis-'Bu1;StiinV Acad;R;P;R; Sect, ;St;Med;; "-1953; ..2; 1, 97. The street rabies virus was inoculated to a group of 33 rats by peripheral route. Nine of these rats had ref- lex epilepsis fits induced by auditory stimulations, 7 pre- sented only motor excitation, 7 were submitted to electro- shock and the other to served as controls. None of the rats with reflex epileptic fits had the disease 32 days after the inoculation. Of the other 24 rats 66% showed. rabies paraly- sis between the 14-26th days. The authors conclude that reflex epilepsis increa- ses the natural non-specific resistance of rats to the rabies virus. 261. ACAD.ST.S.NICOLAU, I.ADERCA, G..DANIELESCU, P.STROESCU. Study of the evolution of rabies with the street virus. In rats" submitted-to_ a" state"'of conditioned sloe ; BulaStiint;Aea:d-ORaP;R; Sect/vSt;lied., 1953,.. 9. In order to remove the toxic substrate that e- xists in narcosis-induced sleep, a reflex conditioned sleep was induced in a series of rats, by means of chloral hydrate and strong light. After establishing the conditioned reflex, the rats were inoculated peripherally with the street rabies virus and divided into 3 groups. The results obtained showed that in,the group ; ;a which extinction of the reflex had, occured, 3/5 of the ani- mals died the as in the group of control animals; in the group in which saline injections replaced the chloral hydrate injections, 2/5 of the animals died of rabies and in the group in which chloral hydrate injections alternated with saline in- jections 1/4 of the animals died of rabies. These data demonstrate the role of the cerebral of t S U~ FAR Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 158 cortex in the reactivity of the organism and the possibility of mbdify,ng. theaevolution of infectious diseases by inhibi- tion of cortical reactivity. 262. ACAD.ST.S.NICOLAU,'N.CONSTANTINESCU, M.DUCA. Investi- gations on the effect of induced sleep-ih-ex.;. perimentsl-rabies; Bul Stii.nir;Acad:R:P:R: - _. SeOt;St:Med;; -1953,' 5, 29-229. A study was carried out in a series of experi- ments on rats on the influence of prolonged sleep on the evolution of experimental rabies. The results obtained showed that sleep induced by medinal has a net influence on the evolution of rabies in rats, prolonging the period of incubation from 11 days in the controls to 14..4 days and even permitting survival of one of the lo animals inoculated. The effect of the state of inhibition is more obvious in the lea in which sleep preceeds the cerebral in- fection. .263. P.STROESCU, I.MIRZA. Modification of higher nervous activity in miee-inoCaldted-WSth--the^entt.: rabiee-vaeeSfle:~St:eePe:tSPPeffiICP08Sd~?yffiSCro- biol:;pat&2ttbl.; 1953, 4-, 3-4, 367. Investigations were carried out on modifications of the higher nervous activity in white mice inoculated with the anti-rabies vaccine, in which conditioned alimentary-motor reflexes had been previously elicited. After inoculation, modification of the higher nervous activity takes place in the first 24-48 hours. These modifications reflect the process of supraliminary inhibition manifested by a prolonga- tion of latency, inhibition of o ti k~r ;, p ei ve cond~,t3.oned reflexes and enhancing of internal inhibition. Certain non-conditioned Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 (defence and orientation reflexes) are more accen:? t! .,~ ~. ~d g which indicates a certain liberation of the subcorti- co 1. regions from under the restricting influence of the cor- '::a ? od7 fic6tions of the conditioned reflex activity lasts ' 'he mean 16-2o days, after which the reflexes come back or al. CONSTANTINESCU9 A.TOMMMA, R0DINUo Excperimental inve stun , avions on the so called "natural"` immunity.. of coo , in rabies iffe tion. St.cerc.inframicrobiol., micro b lol pa.zazitui-o 1954, 5, 3649 269. in 5% of the 15o dogs examined, which were re- f ra-0u L,-, to L epeated inoculations of the rabies virus by ce- r~v route, no serum rabies antibodies were found, not even =t? titers, and the nervous tissue did not kill the virus -; vitro. Since it was possible to immunize the guinea pig :y ,peated administration of subrabies street virus doses, tL natural" immunity of dogs was interpreted as revealing an actively acquired immunity by contact with the sip:': t: virus in areas with enzootic rabies. 265. N . `OidSTAIVTINESCU9 C.DRAGOMIR, E.DUCA, MLDUCA, GR.TEO- DOROVICI.- Strains of rabies virus isolated in Moldavia. Pathogenic activity, fixation capa- cif and antigenic qualities. Stocerc.infra- microbiol..,mi.crobi.olm;pa.razitol0, 1954, 5,3--4, 291. Of the 43 rabies street virus strains - 17 iso- lated from animals s and 26 from man, - with or without anti- none, not even those isolated from human cases considered as due to "unsuccessful treatment" behaved as `?re .~_r_:fnr.ced" virus strains. The cause of insuccess in the persons correctly treated with phenol treated vaccine "killed" Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 16o by heating 72 hours at 37? C was to the method of vaccination or to individual incapacity of immunogenic reacti- vity of the organism. The first of these two factors led to new investigations, following which the classical Fermi vac- cine was introduced in our country. ~c51 13JInfluence of the street rabies virus on higher -nerV613_8" acti~rity in whita ? rats. " 5z a cerc. inframicrobiol.,microbi.oi. parazi.tolo'"1954, 5, 3-4, 359- Motor-alimentary conditioned reflexes were ela- borated in rats, after which the animals were inoculated with the street rabies vi"ru.s. In the first 24--48 hours after i.?o- culation, evident modifications of the Conditioned reflex acti- vity appeared, which were not observed in the, controls inocu- lated with normal brain. This demonstrates that incubation in rabies is much shorter than was thought, and that the cerebral cortex reacts before any other organ in this disease. Modifi- cation of the reflexes points to an early establishment of supraliminary inhibition processes in cortical activity; in- crease of the latency period, inhibition of the motor-condi- tioned reflex, prolongation of feeding time, apparent impro- vement of differentiation-inhibition. The animals in which these modifications appeared suddenly died, whereas those in which they appeared slowly survived inoculation. 267. P.STROESCU, R.PORTOCALA, I.ADERCA, G.DANIELESCU. Resear- ches on septineuritis-in "closed 'rabies". Stecorc. iziframicrohio1', smicrobiol~ aparazitol-. -1954, 59 3-4, 4o9. An experimental histologic study was carried out on septineuritis in "closed rabies", They investigated at the same time the virulence and lesions caused by the rabies Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 161 virus inoculated to animals which had been previously incom- pletely immunized against rabies. The experiments demonstrated that the rabies vi.ruu.;' has an initial affinity for the vegetative nervous system, bDtb peripheral and central, so that the parotid gland, the sympa- thetic and parasymphatetic nerves are often more virulent than the peripheral sensitive motor nerves or even the neuraxon. The anatomo:~gathologic examination shows in the peripheral vegetative system intense rabies inflammatory processes and the presence of a great number of Babeg-Negri bodies. The inflammatory lesions in the neuraxon are to be found at the level of the vegetative centers, around the 3rd ventricle, the bulbus or spinal cord. In the spinal ganglia, inflamma- tory processes of medium intensity and rabic inclusions are revealed. No apparent lesions can be found in the sensitive--- motor nerves. In view of the well known role of the vegetative nervous system in the elaboration of immunity and defence func- tions of the organism, the difficulty of establishing immunity in rabies can be explained both in the course of the declared disease and after anti-rabies vaccination, by the fact that this system is the most vulnerable to the attack of the rabies virus. 268. N.CAJAL, S.MATEESCU. Rapid diagnosis of rabies with the contrast phase microscope. St.cerc.inframicrobiol-g. microbiol.,parazitol., 19551, 6, 1-2, 35. Gior.di malattie infettive e parassit.,195?,2- Since the problem of a rapid diagnosis of rabies is of paramount importance, particularly with a view to es- tablisxi.ing the beginning of the antirabies treatment and since until to-day the methods proposed have, besides advan- tages, many debatable aspects, the authors proposed to find a swifter technique of diagnosis. To this end, they centered upon the method of contrast phase microscopy, which permits Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 162 examination of unstained preparations. The results obtained were excellent; the interval of time required is extremely short: from the moment the fragment of neuraxon from the suspect animal is collected and up to its examination at the microscope, not more than 15 minutes are necessary. Hot fix- ation is performed in a lo% formol or Dubosq-Brasil-Bouin mixture and sectioning is done at the microtome, cutting 2o - 50 /u thick preparations from the frozen products. The slides are then rapidly passed through absolute alcohol and toluol and the cover slide is fixed with Canada balsam. The sections thus prepared are examined under the contrast phase microscope with an immersion objective, after having previously identified with a small objective the layers of Ammon's horn, particularly the central pyramidal layer in which the Babes-Negri inclusions are to be found in great numbers. Identification of the Babeg-Negri inclusions is very easy, eaten for an unexperimented eye and their presence confirms the diagnosis of rabies. 269. N.CONSTANTINESCU, M.DUCA, E,DUCA, A9CHEPTEA. Heated or autosterilized experimental paralytic-rabies i?1 partial,lp mmu,,aized animal,so St?aerc. infra mi.crobiol$s icrobiolotparaz tol.,1955, 6, l?29 9$ In mice and rats vaccinated against rabies with the Fermi vaccine or with the "katadinized" vaccine (M.Duca and E.Duca), the paralytic period of the disease was prolonged up to 9-16 days instead of l-2 days, as noted in the control animals. This prolongation of the clinical phenomena allows the partially immunized organism to defend itself against the infectious rabies virus, autosterilization of the neuraxon takes place and the animal sometimes survives ("healed para- lytic rabies"). Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 163 27o. N,CONSTANTINESCU, A.STIRBU, A.CHEPTEA, C.TAINDEL. The influence of the treatment on antirabies vaccination before and after infection. St.cerc.inframicrobiolOmidrobiolo parazitol., 1955, 6, 3-4, 3750 In preventive vaccination, applied to mice and rats, according to-the treatment scheme with small doses of Fermi vaccine (6 doses at 2 days interval, total dose s 0.37 - o.4o g nervous substance per kg body weight) a more accentuated state of immunity was obtained than with the intensive treatment (21 daily doses, total dose l0.5 g per kg body weight). The intensive treatment, does not completely inhibit the immuna-genetic process and seems useless and definitely unfavourable in antirabies vaccination. 271. G.DANIELESCU, L,MIRZA? Modifications of the higher ner- vous activity in white, mide followin `anti` abies vaccination and subs. izent inoculation of the ., ,_ stre~ t `rabies- virus St cerc o iriframicrobiolo s midrobioia9-para2itol., 1955, 6, 1.2, 55? Investigations were carried out on changes of the .higher nervous activity in mice, in whom alimentary-motor conditioned reflexes had been elicited and who were subsequent- ly vaccinated against rabies and then inoculated with the street rabies virus. Modifications of the higher nervous activity that appear after vaccination are very reduced in comparison with those that appear after inoculation with the non-attenuated live vjruE...(~ In the first 48 hours after inoculation, a state of supraliminary inhibition appears I thelconditione4 reflex activity, manifested by prolongation of latency and duration of the run for food after applying the positive conditioned stimulus, prolongation of the necessary time for ingestion of food and apparent improvement of differentiation-inhibition. None of these modifications appeared in the controls. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 164 272. L.MIRZA EMINET,,J.SAMUEL, E.NASTAC,-C.IACOB. Conditioned reflex activity in dogs during vaceinatian"aOinst and subsequent inoculation with the street rabies. St.cerc.inframicrobiol.,microbiol.,parazitol.,1955, 6, l-2, 65. Alimentary salivary conditioned reflexes were elaborated in 4 dogs, after which the animals were vaccinated with an antirabies vaccine and 34 days later inoculated with the street rabies virus. Modification of the conditioned reflex activity, of the supraliminary inhibition type, appear- ed 13-22 days later. These modifications appear at the mo- ment of maximum resistance, checked by biologic tests. They L. appear more swiftly but more superficially in the choleric type, more slowly but profoundly in the phlegmatic type. In the first 2 days after inoculation, an exaggeration of the excitation process is observed, followed by a process of supraliminary inhibition: prolonged latency, inhibition of the positive conditioned reflex and nonrconditioned reflex, alimentary reflex and apparent improvement of differentiation- inhibition. Disturbances of conditioned reflex activity are more profound in the choleric type, in which they were more superficial after vaccination, and more superficial in the flegmatic type, in which they were more profound. 273. N.CAJAL, G.DANESCU-POPESCU. Research.-- on antirabies immu.ziization in rabbits-with.-modified-te&dti vity of the central nervous" systeM...St.cere. inframferobiola,microbiol.,parazitol.,1956, 7, 3, 311; na c;n gress of Medical Sciences, Comunicarile Sect. Neurol.,endocrinol., Bucuregti 1957, 27. In order to study antirabies immunity in animals whose nervous reactivity was modified by certain drugs in the Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 165 course of the specific vaccination process, benzedrine was used as a stimulating substance and medinal as an inhibit- ing substance. Vaccination was obtained with a phenol treated fixed virus vaccine, prepared according to the Fermi method. Twenty-one days after injection of the last dose of vaccine, the rabbits received a challenge dose by intramuscular route, with the street rabies virus. The experiments carried out showed that the antirabies vaccination of rabbits, performed with administration of benzedrine, establishes a higher specific state of resistance than in the controls. In opposition, medinal administered at the same time as the-vaccination, is followed by a lower degree of immunization in comparison with the controls. These differences may be noted only when a vaccine with an attenuated virus is used and never with vaccines in which the virus has been totally inactivated. 274. N.CONSTANTINESCU, A.STIRBU, C.TAINDEL, A.CHEPTEA, I.STOIAN. Comparative study of the preventive immunogenic _ -taliie " Of phenol. - attenuated and killed"antirrabies-vaccines: Arh:romede"epide- miol:-i-microbial:;"St?ei_Cerc. Edit.Med., Bucuregti, 1956, 353. Comparative study of the immunizing activity in mice of 4 attenuated or killed vaccines yielded the following results, regarding the proportion of animals pro- tected against challenge infection by cerebral route, with the fixed virus (F) or the street virus (S) Fermi vaccine = 29% (F) ; 38% (F) ; 29% (F) ; 52% (s). Phillipps vaccine = 21% (F); 16% (F); 30% (F); 59% (S). Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 - 166 Vaccine killed 72 hours at 370 C ll% (F); 0 (F); 0 (F); 25% (S). Vaccine killed 2 hours at 37# C 32% (S). Controls 0 (F); 0 (F); 0 (F); 0 (S). 275. NQCONSTANTINESCU, A.STIRBU, A?CHEPTEA, C.TAINDEL. Study on the comparative protective activity of the auti,abies ' sePura' atd" of "' fihe~ol" heated.. vaoe.i,ne: .n.. e- pPbim- e ital, St?cetd a infra- microbi,ol , flmicrobio flpa1^azitoi ,1956, Z, 3-4, 423,., Giornale Malattie infettive e parassito, 1957n 2, 231. In experiments on 1,408 animals of various spe- cies, it was.,observed that the antirabies Fermi vaccine does not confer protection when it is administered after intra- muscular infection with the street virus. When the immune serum is administered alone or associated to the Fermi vaccine, a net protection is obtained resulting in survival or prolon- gation of the incubation period and paralytic phase of rabies. These results led to the renewed application in our country of sero-therapy in human rabies. 276. N.CONSTANTIN.ESCU, N.BIRZU. The process of autosterili- zation in rabies. Sty,eerG infrainicrebiol ,micro- biolo,parazitol.,1957, 8, 4, 517. - The National Congress of Medical Sciences, Comunicarile Sea#? Neu.rol-,endocrinol.,Bucuregti, 1957, 29? In animals treated preventively with egfective antirabies vaccines which died however with the symptoms of Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 167 rabies following challenge inoculation with the fixed viruu>, by cerebral route, partial. or total (LD50 = o; autosteri.i i , zation of the neuraxon occurs. Comparatively, in r..- ~rar cinated mice, the titer of the brain is LD5? = lo ' to 10-11._3 6, Some of the vaccinated mice contracted rabies of long duration, followed by recovery. 277? The brain of mice with evident rabies para;'_y sa s, that had been previously vaccinated with a low efficac?y~a. cine (14% of the animals resistent to the challenge inocu, lation), yielded the same titers as the untreated animals which died of rabies (LD5o = approximately to"6"5).: In con- trast, in the animals vaccinated with the Fermi vaccine (58% survivals after similar test-infection), aut;osterilizat'ion of the neuraxon almost constantly occured. 278. ACAD.ST.SJNICOLAU, L.MIRZA-EMTNET, N.CAJAL. Izyesti- gations ka the role of the hi,&her ae ova -gip : in experimental" rabies- and.- the- Pkftepg- Ofd rabies" imnttrtit ~ _ Gto .ale" di~Maiitti.e" i fettive e parassit;fl_295?, 9fl 2a - The res4ts obtained in numerou-is and varied ex periments, ' carried out with a view to studying; experimental. rabies by means of Pavlov's methods are described (condii?: icned. reflexes, modification of-cortical reactivity by chemical and physical agents, etcg,) The data obtained clearly show that both in the case of rabic infection and during the process of antirabi immunization - immunization manifested by a minor disees A,~CONSTANTINESCU, N.BIRZU, GR.TEODORO-VICI N.,STAVi1 .. Prbdess of auto sterilization in rahi~s ; r:.a tially immunized animals. - Giornale Malatti.e infettive-e parassit;-1.57, 29 229. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 168 the central nervous system plays an important part. This was demonstrated particularly by means of the Pavlov techniques, which are an excellent means for revealing early modifications of the cortical reactivity caused by infection, modifications that appear long before any detectable clinical symptomatology. 279. ACAD0ST.S.NICOLAU, RQPORTOCALA. Rabies with delayed incu- batiOn in vaccinated subjects:" Gibtriale'di-Malattie it.fettive._ e-patassit?; '1957; - 9, 2, 22o. The National Congress of Medical Sciences, Comu- nicarile SecV.Neurol.,endocrinol., 1957, 59?. Investigations were carried out on 3 cases of hu- man rabies with delayed incubation (158 days, 738 days and 6 years) which occuraduin inoculated persons. The pathogenicity of the strains isolated on animals is fairly low. Microscopic examination of the nervous system in the 3'cases showed, besides the usual modifications, typical rabies inclusions in the cytoplasm of the glial cells, white cells and vascular endothelial cells. These inclusions were likewise discovered in the nuclei of the'glial cells in Ammon's horns. 28o. R.PORTOCALA, P.STROESCU, N.CAJAL. Histopathologio study of the vegetative nervous APteia" ib.. rabies:Gior- nale di Malattie'infettive`e-patassit:;'1957,9, 2.25. In the course of rabies, due to the street virus, the presence of numerous rabies inclusions were revealed in man and animals in the ganglionic neuron of the retina and in those of the carotid glomus. In the partially immunized animals, similar in- clusions are to be found in the vegetative ganglia, The same Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 169 findings are likewise valid for the nervous intra-parotid microganglia. The peripheral vagovsympathetic system of these animals is virulent, which demonstrates that immunity cannot be obtained without integrity of the vegetative sys- tem. It is assumed that the alterations described explain to a certain extent the vegetative and psychic dis- turbances encountered in rabies. 281. N.CONSTANTINESCU, N,BIRZU. Investigations on the me- chanism of autosterili~,atien-..." rabies; ..' " _. ag 19589 2e 2, 331. The brain of vaccinated mice completely destroys the rabies virus in situ or inhibits its multiplication in vivo. The inhibitory activity explains by itself the total-or partial process of autosterilization of the neuraxon. The brain of mice passively immunized by antirabies serum,does not exhibit this rabicide capacity. Once the virus has pe- netrated into the neuron, it multiplies - sheltered from the circulating specific antibodies - as if immunity did not e- xist. 282. N.CONSTANTINESCU, N.BIRZU. Phenomenon of autosterili- zation and recovery in.eperimental rabies_ Anzi;de" l1Inst0Pasteut, '1958I _94 - 739.... Antirabies vaccination prolongs the period of incubation of rabies caused by challenge cerebral infection. Once rabies has appeared and when the animal is vaccinated preventively, the autosterilization of the neuraxon almost constantly occurs. This process of autosterilization may sometimes lead to "healed paralytic rabies". As a rule, rarief, behaves in the partially immunized organism like any autso- sterilizable and curable neuroviral disease. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 17o 283? ST.DRAGANESCU, A.PETRESCU, N,DRAGANESCU. Inclusions of the Babe .Negri type in a case of - ascending paralysis not determined etiologically in the c,iini a Stecerc.neurala 1958, , 2, 161 In a case of ascending encephalo-myelo-'radiculo-n neuritis, considered clinically of a non=determined primary viral origin, with albumins-cytologic dissociation and with- out antecedents of rabies infection, intracytoplasmic Babec? Negri inclusions were revealed in the cells of the cerebral cortex,. the Purkinje and Ammon horn.. cells, besides the non- characteristic infiltrative processes in the nervous system. The hypothesis of encephalitis with Dawson in- clusions seim, s very little probable, the virologic diagnosis remaining that of rabies infection with a clinical character unusual in the human infection with the street virus. 284? ACAD.ST?S0NICOLAU, N.DRAGANESCU, CLJNICOLAU, N.I.IO- NESCU. Investigdions on a new type of anti- rabies vaiiae'with" sil,rer" ihs~ _Staeerc ?in? framicrobiolo?micrObiblogpaia2itele, 19589 ,2v 3, 315. A new method for preparing the vaccine with fixed rabies virus, inactivated with the aid of silver ions is described. This can be obtained by electrical concentra- tion with silver ions and also by mixing the virulent rabies suspension with certain silver salts and particularly with silver nitrate, In a o.15% solution in distilled water, this salt adequately attenuates the fixed rabies virus. By adding an aqueous suspension of "silvered" virus, to an equal volume of 18/boo sodium chloride solutiol, potent antirabies vaccine is obtained, whose immunizing potential. is at any rate equi- valent to that of other antirabies vaccines prepared by other methods. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 171 285. ML.BAB.ES. On the beginnings of Rumanian rabiolo y. St. cerc: inframicrobi.oL ~ ; " 1959; "Zo ~ " 2; ^ 24T, m Victor Babes, the founder of Rumanian rabiology, was the first foreign investigator to be personally initiated by Pasteur in Paris (January 1886) in the details of anti rabies vaccination in man. Babeg's research work at the Pasteur Insta.tu.t,F, immediately suggested to the Rumanian investigator the idea of studying the a"gnomonic histopathologic signs of rabies, the mode in which the rabies virus propagates along the ner? vous pathways, the method of attenuating the virus by heat, trepanation of rabbits with the gimlet instead of the crown- trepan, the swifter transition of the street virus into the fixed virus by the use of guinea pigs, etc,. Rumanian rabiology greatly benefited by these discoveries of Babeg's, already from its beginnings (May 6, 18880) 286. N. CAJAL, G?DANESCU- POPESCU. The evolution of expo mental rabies and antirabies m~an~f; dhUt- the action- of-dorti exo; 4-' -The -Sed6hd-Cohgtess of the Hungarian Microbiologists, Budapest, September 22-=26, 19590 Investigations on hamsters, mice and rabbits, concerning the influence of cortisone on the evolution of ra- bies with the fixed virus or the street virus and anti rabies immunity obtained with the Flury virus, or with a vaccine with inadtivated fixed "Babes" virus, led to the following conclu- sions Administration of cortisone at the beginning of the experimental rabies infection increases the sensitivity of the animal to the pathogenic action of the street virus and, to a certain extent, to that of the fixed rabies viru.sm ?Thus, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 172 the period of incubation and of survival is reduced, the dif- ferences being much more evident in hamsters, which as a rule contract rabies of long duration. AMrabies immunization in rabbits is more ac- centuated under the action of cortisone although the sero- neutralizing antibody titer is lower. In this way, the ab- sence of a parallelism between the degree of inframicrobial immunity and serum antibodies is again demonstrated. 287. N$CAJAL, G.DANESCU:POPESCU, Investigations on anti, _-._- rab ,es immunity in rabbits-V& dirlated -With h--the "11'a jr" strain ? under the infliuence - of - cordsote . Stocprc.inframicrobiol., 1959,~1O;4; 447: A study of the degree of immunization induced in rabbits by the avian adapted "Flury" rabies virus under the-action of cortisone acetate, and the dynamics of sero- neutralizing antibodies, showed that immunogenesis is signiw ficantly accentuated under the action of this suprarenal hor- mone. The influence is more obvious when cortisone is admi- nistered 2 days before vaccination and then 2 more days, coincident with the "Flury" vaccine. The degree of immuniza- tion is independent of the titer of the serum neutralizing antibodies, which definitely decrease under the action of the corticoid hormone. 288. N?CONSTANTINESCU, N?BIRZU, I".MICU, O.ZAVATE, FL.VASOIU. Process of autosterilization in human rabies. Stooero;Stiint;;tedicin ;'Acad:R:P:R:"Pil:Ia i, 1959, 1oD 1, 1290 The same process of autosterilization is ob- served in human rabies as in animals. The cerebral tissue" in non-vaccinated human cases yielded titers of LDSs =lo .3 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 173 to to 5009 whereas in vaccinated persons, who however deve- loped rabies, the virulence of the brain only attained LD = 10-1?3 to 10-2"5? Since the possibility of the inter- 50 vention of a. naturally attenuated strain is excluded, these low titers reflect the process of autosterilization in vac- cinated persons. 289. N.CONSTANTINESCU, N.BIRZU. Is not rabiesa curable disease ? La'Irlesse M6dicale-1959;'7,98 33i Considerations are made on the autosterilized or healed rabies infection in naturally resistant or sensi- tive animal species, on 'inapparent infection, rabies septi- neuritis and "closed" rabies, This behaviour of the disease implies the necessity of renouncing - in the case of human rabies in vaccinated persons - to euthanasia by morphine and of adopting an adequate symptomatic therapy, as in the bulbar form :of poliomyelitis. 290. N.CONSTANTINESCU, N.BIRZU.y IJMICU. Research work on the mechanism of protective-activity of the antirabies set='I Stocercoinframicrobiol,1959, lot 3, 297.0 The protective activity of antirabies serum is conditioned by direct contact between the rabicide antibodies The virus is neutralized and the infectious rabies virus, only as long as it has not penetrated into the Nervous system; once it has penetrated - the intraneural phase - the action of the serum becomes nil. These results implye that, in hu- man therapy, local serum infiltrations should be done directly in the anatomical region infected, at the same time as general serotherapy. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 174 291. ACAD.ST.S.NICOLAU, B.FUHRER. Investigations on the presence of the street"rabi :virui?'it- the" blood-of a eritnentally' infected' rabbits... Report-read at-the_'Festive-Sciefitific'Session of the Institute of Inframicrobiology,.. August 17-18,.1959. The existence of a period of.viremia in experi- mental rabies in rabbits and guinea pigs was demonstrated. This period occurs between the 4th and the 7th day after inoculation of a virulent suspension by intramuscu- lar route. The presence of the rabies virus in the blood of the animal was demonstrated both histologically and*bio- logically., 292. ACAD.ST.S..NICOLAU, L.MIRZA, P.ATHANASIU, AL.PETRESCU. Is it possible to determine by'meana of'the conditioned-refie2'>aathOd-the limit of rabies ?iras-variabtlitg-in^formolated-antirabies- vaccines^? The experiment was carried out on lo groups of 4 rats each,'with previously elaborated conditioned ref- lexes. Six groups were vaccinated with the variously atte- nuated antirabies vaccine (formolated o.5/1,000, 1/1,000, 2/l,ooo, 4/1,ooo, 6/l,ooo, 8/1,ooo) the other groups sarv- ing as controls for the reflexes, for the virus and for the 0.5/l,ooo amd 8/1,ooo formolated protein. The experiments showed that no changes occur in the reflexes of,the con- trols for reflexes, of the controls for non-virulent homo- logous 0.5/1,00o and 8/l,ooo formolated protein,'and the rats vaccinated with 8/1,ooo and 6/l,ooo'formolated anti- rabies vaccine (prepared with homologous protein). Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 175 The' reflexes are-''~however slightly upset in the rats vaccinated .with- 4/1,:000-- form?lated vaccine and * strongly disturbed:.'in rats vaccinated- with 2/1, ooo, -1/1, ooo ' and'.- 095/l,oob-formolated vaccine; like-'in the,controls for the non-attenuated rabies: virus..: -It 1s " known' that'. potent vac- cines in the-field of-,v.iral.diseas?s contain at: the utmost. 4/l,ooo formol. ,.A.-vaccine with. a.. higher: concentration of-. formol becomes- jion-immilnizi4g.t,,:.non..,operiktive. It may be' assumed that ,a higher concentration, . of: formol denaturates. the. chemical ,,constitution of .the germ.and;. destroys- ito The- dead virus, is inactive against,conditioned.reflexes. as may be seen from the .. above,,experiment....,,This supports the-idea-.. ,that the met hod- .. , of..conditioned .reflexes is a good experimen- tal 'scheme =for assessing the degree. of ,viability of a virus '. contained in .avaccne and.its immunogenic potential 293..' ACAD.ST.S-NICOLAU, N.DRAGANESOU, CL.S.NICOLAU,'N.I. IONESCU. Investigations on a new -type of antirabies yaccih6-o-"Report"'read at the"-Second .Congress, of:-the Hungarian - Microbiologists, Budapest,,. September . 22' 26,, 1959.; heavy'metals, an effective antirabies vaccine was prepared for mice.-and.-rabbits,. with . a. -silver nitrate.. . A lo% sus-- pension ..of fixed rabies .virus, Babe 0-, strain, in a. 0.15% sil.- ver nitrate solution,...-left ,.24hours at---.room,. temperature -in. the dark,, -becomes vaccinating and.; confers..to the, white, mouse. On,.the,basis of the oligo-dynamic. action of With a view to long-term storage of -the vaccine. thus prepared, to.the., drained off supernatant ;or after centri- and the rabbita solid resistance to.ingection;.with.the fixed.-virus .by,, cerebral route. way, -_an antirabies .vaccine .is.. obtained with an. efficacy on mice.and .rabbits ,that lasts more than 3 months. fugation. an. .equal .volume, of saline, solution is added. In this Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 176 Comparative investigations' on the 'efficacy of this vaccine and that of the Fermi vaccine have shown its superiority in mice and rabbits. Dialysis 48 hours against distilled water at 440C of a suspension of fixed rabies vi- rus in 0.1.5% silver nitrate solution,,showed that in the working conditions applied, attenuation of the fixed rabies virus seems to be an irreversible phenomenon. In order to study the mode of spread and accumu- lation of this vaccine in the various tissues of the immu- nized animals, investigations were carried out with Agll0e TYie vaccine was prepared in a NO3Ag1 0 solution and the radio- activity of various organs was then determined, The results show that the attenuated virus penet- rates into the nervous tissular system, which it immunizes, through the homologous sciatic nerve. The conclusion may therefore be drawn that this vaccine follows the centripetal route of migration characteristic of the live neurotropic viruses. 294. ACAD,ST.S.NICOLAU, N.DRAGANESCU, CL.S.NICOLAU, B.FUHRER, E.GIRD, N.I.IONESCU. Experimental researches anti.r.abie'~~ac5,.fieo Arta" Viro? on "silvered" logica, 195931.91,_"RevoSeioMed.,l959, 81. An antirabies vaccine, prepared with the fixed rabies virus attenuated by means of a 0.15 % silver nitrate solution, was demonstrated to be as effective in mice and rabbits as other vaccines prepared with the attenuated virus. Migration in the organism of the radioactive vaccine prepared with Agl10N0~ was studied. Radioactivity of the sciatic nerve in the inoculated thigh was higher than in the 14 other different tissues studied, thus demonstrat- ing the neurotropic affinity of the attenuated virus contained in the vaccine, although the latter was not pathogenic for mice on intracerebral inocu.lati.on. The viability of virus particles in effective vaccines and the results supporting the views on tissue immunity in viral infections are then discussed. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 177 295. ACAD.ST.S.NICOLAU, NODRAGANESCU, N.I.IONESCU, M. ANDREESCU. Investigations on the preservation of the efficacy of the "silvered" antirabies"" vaccine. Comparative study of the immunogenic effect of the "silvered " vaccine and Fermi vacs St.cerc.inframicr.obiol., 1959, lo, 3, 2670 An antirabies vaccine was prepared with o.15 per cent silver nitrate solution. Twenty-four hours after preparib4 the vaccine, a metallo-protein precipitate is formed: silver-cerebral substance of rabbits infected with the "Babeg" fixed rabies virus. By eliminating 24 hours la- ter the supernatant fluid - by simple draining off or by centrifugation, and adding an equivalent amount of saline - an antirabies bvaccine is obtained whose efficacy in mice and rabbits lasts more than 3 months, which proves, in con- trast with the results obtained in previous experiments, that the factor determining decrease of the protective action of the vaccine is to be found in the supernatant. Chemical de- terminations showed that to ml supernatant contains o.oo16 gm silver or o.oo2.5 gm silver nitrate. The infinitessimal amounts of chlorides and NO3 ions present in the supernatant, denote that the existence of excess silver ions constitutes the factor that determines the loss of the efficacy of the virus with time. Comparative researches on the efficacy of "sil- ver" vaccine, prepared by eliminating the excess of silver ions after 24 hours and of the Fermi vaccine, showed the su- periority of the former. Diilyses during 48 hours against distilled water at +4U of this fixed rabies virus suspension in o.15 per cent silver nitrate shows, in the experimental conditions of the authors, that attenuation of the fixed virus can be an irreversible phenomenon, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 178 296. ACAD.ST,S.NICOLAU, N.JJRAGANESCU, CL.NICOLAU, N.I. IONESCU, B.FUHRER' E.GIRD. Experimental inves- tigations on silver antirabies'-7aecine; .. -ReV,- - Sci,Med,, 1959,:49-816' An efficient a t.irabies vaccine for mice and rabbits was obtained with silver nits?ate in 0.15% concentra- tion. With the aid of this vaccine, the ascending migration of the virus-%raccine along the sciatic nerve to- wards the central nervous system, which it immunizes, was demonstrated, by calculation of the infectious activity of the-various organs. 297, ACAD.ST.S.NICOLAU, N.CONSTANTINESCU, N.BIRZU, O.ZAVATE. Immunity in rabies appraised by the peritonea', neutralization tests St.cerc;1nframicrobiols; l96os"ll--I;'9?,--'Acta Virol.Ung. 196o, 7, 2, 184; 'Biull.Nauc.inform. 196o, 2, 131. The correlation between rabies antibodies and the evolution of rabies was investigated,* substituting the classical neutralization test by the "peritoneal neutraliza- tion" test, To this end, the animals (hamsters, rats and mice) immunized against rabies with the Fermi vaccine and which had finished the series of vaccinating injections 1 to 163 days previously, received by peritoneal route a 10-2 suspension of cerebral substance from rabbits infected with the fixed Babel rabies virus. After 6o to 9o minutes, the peritoneal fluid was sterilly collected by puncture and inoculated intracerebrally to 5 mice.. The state of immunity was appraised by survival of the animals in the experimental group. The results obtained demonstrate that in comparison with the animals of the control group, that died of rabies encephalitis, "the peritoneal neutralization test" is very precise in showing the state of antirabies immunity and the Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 decli.ne of immunity, G s ",i alss af trer boos, t? r .o re i.~.7,~e?.lo'ii'-:'.~.[4'.,. f.,7'r.`~.~,? - .A.!:l ~.. ~m'ad~e evident o:~" I__7 of the 'Vr .rus.-. 298'. 1VoCONSI'ANTINESCtT9 "'N.CAJ'AL9 ' N..BZRZU9 M.CEPLEANU', 0 ZAVATE. Experimental rabies infects in the Syrian hamster: St. cerc. i lfran~icrobio1.196o" 11,`' 1, 61^; Adam Virol.Ung.196o,- 7,, 2, 184. 0 L, Study"o `t`he evolution of experimental rabies infection in the Syrian hamster,showed. that this - animal .. - species ' is ' vary 'receptive both to the `street and the fixed rabies virus. ' In -contrast"'to other sensitive animala, the to 20 days. paralytic phase in the hamster is exceptionally long, up bodies in loo per cent of. theneurons, with a characteristic internal structure --;instreet rabies virus; numerous in- clusions-of-variable size;:formed - of_ homogenous oxyphil mass, situated in, _ the,.. neurons of Ammon''s horm- and --of' the ~. hippocampus :in, fixed "Babel",..virus.rabies. ticularly intenseilarge and. sometimes giant Babe?-Negri -By'prolonging the conflict - between the virus and the organism, a process*of"autosterilization develops in a number: 'of 'a'nimals. similar to that observed in other laboratory 'animals' partially` immunized by antirabies vaccina- tion. In ~this-species, " the inclusiogenetic process is -par- x.79 : ;y' r..n hamsters and rats. ,:Grua.: l: -' attenudteo byy long inter- t,_ .'s:o9 m_ y e r ~. t ;:vated by 'tralization. test Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 299. C.SURDAN, E.DUMITRIU-CAROL, C.CURE, M.ANGELESCU. Contributions to the study of post rabies vacci- nation neuroparalytic complications in the*doG? St virus which does not cans , self or because it immunized sub jec .s, tion is, as a rule, bo d wes, may be absent, either s i., e case is due to an "enhanced" u' e appearance of inclusions by it- e in... vaccinated, i.e. partially 1 -,hieh the process of inclusion forma- i_:ryhibited in its development" 3o8. I.:BERLOG.EA, I.ST TI, &SCHOENFELD, NOCAJAL, G.POPESCU. 1' , e t ; dns on rabiess infectjo . and rabie3 a-m,-,r? ty in an imaIa subjected . to irradiatioh,' SL1G~:;rrt'i'X3.fa.`IiI,.`~p16.1 f' In e- p riwents performed in white mice and white rats, i--rrad i atior. of the animals with 450-5oo r was found not to alter their susceptibility to the inoculation of va- r? ous rabies strains ("Babeg" and "Pasteur" fixed rabies V ;..ru e-, s e: ^.?e.L ?'iru 9 "Flury" virus) o Consequent to the introduction of "Flury" virus, resistance to rabies is slower to set in irradiated animals. at the :~n:1- of 20 days, it will not differ from that ob rci ed. Ic. non.- irradiated animals. A .- I RhVICIq ml, --SICULESCU, M? GRUTAO Benign lyan.phoo tic menin itis~ Experimentation oi.' an n.a soStoeePc. iui?ra:aicrobio1 9 M11950; ..ls '2; I.oSw - Th.e etiologic agent of certain non=-bacterial Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 meningitides: is investigated. Fourteencase s, exhibiting the`: Colentina? Clinic of Contagious Diseases. symptomatology of lymphocytic meningitis-,wee-,-studied in the -at ona "' ~7Sse ~ s' mittahle i i serf"4l1 passagesby In the experiment, 122'guinea pigs, 64 mice and 1;2.,rabb is were sed:o From the cerebro. spinal fluid and the blood, of the ,pat ents~;a causative. agent wasp isolated,;. capable of .nd cirwg ,~ 'hair- disease: in: g . nea i s' on~.6 of;;the?p. case S. after' 6cillitioh and in 261o the , ws s s ., .:~..Lce- ret aee.d 'the 1:8=_'P6tb:.' 3ay:,~after inoc'u`= t.,_ a; T. ~,:RtTIA The ~- k 3 ~ ? ' ~l . ~ , ?,~ -,:,~;SCU Q i T . - -~ - grope rt"e s- lung triturates. of the etiologic .gent of lymfihbeytic-neningitis? St corca LT c robjol, 195o; 1, 2, 155. ai'rir.fl _..s., .~-n2$ "7T' The authors investigate the portal.. of entry and route of propagation of the benign lymphocytic meni.n- gitis virus, isolated by the authors, who demonstrate the role of the mouse as vector of the. disease, this animal be- ing found in the house of 24 of the 28 cases investigated.. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 l91 317. IpSA!i~NEL. Cho.rc me.:i it: s strains isolated in . : lea s in 1954 10;2350- C omuniearile Acad. R,P.R. , l956 - .. " 6, 5, ?.3 ;. Du r. t.. ? g t. h c; period, 8 meningitis strains were isolated by _io,c,ulation to mice of cerebro- spinal fluid drawn from .9 ca ~:. es with a clinical aspect of benign meningitis. The possibility of breeding contamination among mice was excluded. Except for a single strain, isolated in July, all the other strains were isolated in autumn 1954 and 1955. The clinical aspect of the disease, the symptoma- tology of the experimental infection and the anatomo-pathologic picture of the animals which died following this infection, are those of a benign lymphocytic chorio-meningitis, of the Arm- strong type. 318. I.SAMJEL? Eti?logic researches in viral meningitis, Gioth;M&iatti.e`itfet'Give"e"parassitQ; 1957, 2.9 An attempt was made to isolate the etiologic agent from 115 patients with a clinical diagnosis of aaeptic menin- gitis. It was possible to isolate 11 strains of chorio- meningitis virus by inoculation of the cerebro _3-'spinal fluid to white mice. The isolated strains were obtained from 9 adults, -. adolescent and a child , in whose homes the existence of mice had been observed. In another 3 cases (an adult and 2 children), the Coxsackie virus was found in the feces of the patients. Four strains of mumps virus were isolated by ino- culation of the cerebro-spinal fluid into L-,:he am_rictin' Mav y o :f 7'-day chick embryos. One of these strains was obtained from the cerebro-spinal fluid of a child suffering from mumps, without any clinical signs of meningitis, but with spinal fluid reaction. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 192 319. N.DR.AGANESCU? Study mrf an i,: fra!nicrobi,al germ isolated from a ease ..f''~i,gn?~~' Ltf'F-.wti~'Sst~fli?2;1ti.So S.t.cer-c- .. ram icr oyd6I ~Az'i.br6bi6l,; 'b&ir&ZitoIo, 1958 39 -L L 6 .L eir(.,SMed., 1.958, 3, 2( An inframicrobial germ was isolated on embryonated eggs, from the ce.rebro-spinal fl.aaid. of a patient with multi- recurrent meningitis. From the of its characteristics, this inframicrobial germ: .:..sated, like the M C virus described by Mollaret and Cateigr.e, only on the chorio-allantoic membrane of embryonated eggs. Inoculation of the cerebro-spinal fluid by intra- cerebral route and the scarified cornea of the rabbit, as well as intracerebral inooU.lat_or to mice and guinea pigs, were ne- gative, woich rules out the possibility of a chorio-meningitis or herpes virus. The isolated virus did not have hemagglutinating properties, but induced intracytoplasmic inclusions in the layers of the chorio-allantoic membrane. The embryos hatching from the eggs inoculated by intraamniotic route, exhibited cerebral hemorrhagic le- sions without the presence of inclusions. 32o. ST.DRAGA ESCU, N.DRAGANESCU, M.I.POILICI. Acute primary choreiform'encephalitis with inclusions; Presse' Med ,..1958; 66.48-..1693_.. A case of acute primary choreiform encephalitis ending in death 28 days after the onset is studied. The morpho- logic examination of the brain showed only discrete lesions at the level of the white substance (lipid di.;integration, slight perivenous glial proliferation). By means of the Mann staining method, typical intranuclear and intracytoplasmic inclusions were revealed Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 193 L n 'the nervous cells and famphophilr corpuscular matk Pons in the filial nucleiL. -The presenae-.:of these inclusions showed the viral origin of this encephalitis, the nature of which was not determined,. 321. ST.DRAGANESCU, D..SARATEANU, N.DRAGANESCUr Gamvlement. fixation; with' the tick-borne jvirus> tCcPraga -antt .mammon- No e )l in Ygariaus:,forms of,~-panenccephaalr t.44s::- Neurol., psY ihi.,~ neurochir. 1958,-2--163-,,---- The results of investigations carried out on the -.omplement-fixation test with the sera of 27 patients suf- r. from various forms of panencophalitis and from ~8 iaps? V~ : ently heiallthy ?persons, from the .ho;c:ality 1,n wh1ich :,-cases of encephal it.is ' ha'd .occured 2 years previously, are presented. In 4 of the 14 cases --of p.anenq+ephaliti~s in various stages of the dis'e-arm, ;.positive complement fixation reactions were obtained with the -sera collected .1-3 months .after the onset of the affection. The complement fixation reaction -was posi- tive in a single ,c~awsgj. ft ut : .1'ateva=1 scl:.er.o!s s) -of .the 13 napes of per3, hk aFl pane. .^ 4,a~l :t.is -studied semolog tally. Fn 3 of the ~$ =a~ppa~r-entbl~y phe:aalthy' c.a.sees,, the complement f ix- r,.. n .react:%an was positive-* ST*DRAGANESCU, I.VOINESCU., N.DRAGANES.CU.. P .ch? o Ic enceephaa-lit s,. Isolation: of .,the, ytirus-,-~Repc:rt read at the Session;-:of" the".Society .bf`Medical ,S.cfiences, Section of Neurology,:Bu4ll,a:cosrV,, yS%p.tember :24,1958. A clinical case of psychotic encephalitis with a favourable evolution is described, The disease began in April 1958, with anxiety, head-ache and fever. The patient was hospitalized a few days after the appearance of the first symptoms in a psychiatric ward, where a diagnosis of schizoid syndrome was made. In hospital, the patient exhibited a mode- Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 194 rate subfeb.L iL: state, with a fever-peak of 4o.20 C and a convulsive fit. From the neurologic point of view, slight meningeal phenomena were observed, paresis of both m.rectus externus, a right facial paresis of central type, slight modifivation- of the osteo-tendinous reflexes and motor de- ficiency. Moreover, the patient was confused, with slight desorientation and catatonic tendencies, Intracerebral ino- culations to white mice of the cerebro-spinal fluid withdrawn in the regressive phase of the disease determined in this animal species . ?:L_Lv - ...,,-siomena of encephalitis, serially transmissible. 7.23,, ST.DUGANESCU, S.VOINESCU, A.PETRESCU,' N.DRAGANESCU. Diffuse neoformation process with encephalitic .~e r aspects, St?ce~c;ne Oi:, 958; 4;"375: A case clinically labelled as pseudo-tumoral encephalitis is described. The morpho-pathologic examinadion d diffuse sarcomatosis Of the cerebral blood verse actual perivascular sarcomatous encephalitis proses; he various regions of the brain, gaving however a t- o a:. :hickness in the subthalamopeduncular area. Cytologic study the infiltrative processes in the meninges and nervous parenchyma showed polymorphism and the character of the va- rious tumoral cells. On the sections, stained by means of Mann method, numerous eosinophil inclusion- like- form Nations of various sizes, some of them with a halo around them , were observed within the nuclei of the tumoral cells. These are chromatic formations, since they are positively stained by the Feulgen method. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 195 32!~a belonging to the arthropod borne grvapIdea- tification of the causal agent and study of the nervous.lesions inducedin the white mouse St.eerc..inframicrobiol@,1959, lo, 3, 3630 An encephalitogenic inframicrobial germ, patho- geaie for the white mouse was isolated from the cerebro- pinal fluid of a patient from the Dobrogea, admitted to the ,11-nic with phenomeiia of psychotic encephalitis. It was proved by the sero-neutralization test against.the standard encephalitis serum (strain Hy PR), that the isolated germ belonged to the group of tiok-bonne ;._ . ahalit ide s . Auatomo-pathologic examination of the brain of the mice whioh died following the experimental-disease showed a pawn-encephalitis, characterized by a process of perivascu- , inifF I t:;-.-at' ion, glial proliferation and neuron ophagic no- dules. N,DRAGAIVRSCU, inframierobial meningo-encenhalitiiss N?DRAG ;r ' 3OTY. Virological determination of spring summer tick-borne encephalitis. in the Rem perime ntal researches on isolated strain. Report read at the Second Congress of Hungarian Microbiologists, Budapest, September 22-26,1959? An encephalitogenic 'inframicrobial germ, patho- g-nio for the white mouse, was isolated from the aerebro- spinal fluid of a patient from the Dobrogea, hospitalized in the clinic, with phe:omena of psychotic encephalitis. The sero-neutralization test in mice against the standard ence- phalitis serum (strain Hy PR) showed that the isolated germ belonged to the group of Russian tick-borne encephalitis vi- Anatomo-pathologic..examination of the brain of the mouse, which had died following the experimental disease, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 196 showed that it was an ence,~,.aalitis charac Lerised by a pro- cess of perivascular infiltration, glial proliferation s,^l neuronophagic nodules. Inoculation of the virus by respiratory route. to mice induced,,, both clinically and morcho-pathologically, pneumo-encephalitis lesions, characterized by interstitial lesions at thelevel of the lung and by inflammatory le- sions throughout the neuraxon, specific of this viral di- sease. Four serial Intrapulmonary passages multipli4d this virus in the pulmonary tissue, without giving it how- ever strictly pneumotropic properties. Intracerebral inoculation to the young and adult hamster determined clinical phenomena oi'encephalitis, cha- racterizad by paralysis of the hind quarters, without con- vulsive tonico-~clonie fits or muscular fibrillation. The anatomo-pathologic lesions are much more intense than in the white mouse and are characterized by a meningo-encephalitis process. 326. N.OAJAL, Y.COPELOVICI, M.CEPLEANU. The action of amniotic.and all.antoic.fluids of birds on the . MM encephalomyocarditis virus. Lucrarile celei de a 11-a Sesiuni pt. a I.M.F. Buc., 13-15 april. 1956, 91. In view of the results obtained with the influenza virus, investigations were carried out on 362 mice in order to establish if the amniotic and allantoic fluids can be used as diluents also in experiments with the MM encephalomyocar- ditis virus. After inoculation of the virus by intramuscular route, it was found, as in the case of the influenza virus, that amniotic and allantoic fluids - especially the former - produce an increase in the virulence of MM virus, assessed Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 197 by the LDS0 in comparison with dilutions in r r,1 saline. The mechanism of production of the differences observed appear to reside in protection of the inoculated elementary bodies by the embryo fluids against the ithmediate defence action of the organism. X27. L.MIRZA-3MINRT, I.SAMURL. Cortical reactivity in case of ininap arent infection of the MM type inwhite rats.. St.care.inframicrobiol.;microbiol.*,para= zitol., 1956, 2, 3-4, 327. An attempt was made to detect i apparent in tti43tion of the MM type, with the aid of conditioned reflexes. Oka this end, alimentary motor. conditioned flexes were ela- borated in a group of 2o rats, after which the animals were inoculated with the encephalomyocarditis MM virus. Only 2 rats exhibited transitory paresis, no evidence of the disease being observed in the rest of the animals. However, signi- ficant modifications were observed in their conditioned ref- lex activity during the first 24 hours s prolongation of la- tency, inhibition of the motor conditioned reflex and non- )nd.itioned alimentary reflex and an apparent reinforcement of differentiation-inhibition. These disturbances did not ;.pear in the controls, which meant that although the cli- nical signs of the disease were likewise absent, the animal 4P,d contracted an inapparent disease, detectable by means of the method of conditioned reflexes. 328. 1.ADBRCA, M.IANCONNSC1T. Cultivation of the MM enceuhalo- _ Ids m-..o?~ myoeardiac virus in roller-tubes; St;oorooinfra- m.icrobiol.,miarobiol.,p&razitol:,1957, 9919 49, MM encephalo-eyocardiac virus was cultivated on neuronal tissue cultures of mouse embryo, by the roller-tube Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 198 ~.etho0 `?'r> virus constantly produced a cytopathic effect, in the cultures of proliferative cells, Six serial passages were carried through with a theoretical end dilution of 5xle 100 The attempts made at washing the virus off the cells, showed that it is constantly adsorbed within 15 minutes,, the washing not preventing the action of the virus on the ce i l'i. Tntracerebral passage in mice of the culture fluids from each passage showed a decrease `?a titer due probably to attenua- tion of the neYIRZA . Cortical reactivity in anti-influenza immu- nity processes.._- Report read at the' Society of Medi- cal Sciences,Bucharest, October 19,1956: One of the:several aspects of immunogenesis processes, was investigated,i.e. the reactivity of the cerebral cortex during anti-influenza immunity. The experiment was carried out on a group of 3o rats. Cortical reactivity was studied by means of conditioned reflexes. When the reflexes were well established, the animals were vaccinated with the an- ti-influenza vaccine. In the first 24-48 hours after vacci- nation, a powerful state of supraliminary inhibition was ob- served in the conditioned reflex activity. Periodically the reflexes came back to normal, an inhibition--phase appearing soon afterwards, the conditioned reflex activity thus taking on an ondulating aspect. A parallel study of this activity and of the titer of antibodies showed that this titer did not increase to the same extent in all the animals and that there existed a strict parallelism between this factor and the severity of the disturbances of conditioned reflex ac- tivity. 372.- D.SARATEANU, I.NICOARA, EIZICOVICI, T.VIRF, COVACI. Dynamics of s6ru .anti-infl uenza- antibodies in--vacci- nated persons in the- tbWa'- bf-- le Soc. Stun '.fined:. T~:ffiti re$" 'ebraary 8,-19 9 56. The results of serologic investigations in 277 cases of vaccinated persons and 266 controls are reported. An increase of anti-influenza antibodies is observed for all virus types (A, A', B), the increase being higher for type A and lower-for types At and B. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 ?227- The highest antibody titer was noted 2 weeks after vaccination, decrease taking place approximately 2 months after vaccination. Although epidemiologic tests were not. carried out, the authors conclude that in view of the innocuousness of the vaccine, prophylactic vaccination should be recomended as the: most effective method for the control of influenza, 373.- DaTARCHILA, CH'MARINESCU Laboratory.diagnosis-.-Gf the severe forms of influenza in' infan.tsq O.i S.r1. - '~_onal Conference of Pediatrics; - Fehr ua31-8~1o e1956, 257 ? The laboratory diagnosis of influenza in small infants is of great practical use, particularly in the severe and atypical forms. Difficulties are however encountered due to the particular serologic reaction of the small child and to the collecting of.pathologic products at this age.. The la- boratory diagnosis of influenza in the small infant'is ba- sed upon specific elements ( the causal agent and serologic reactions) and nonspecific elements (le ogram,etc.), The presence of the causal agent can be detected by morphologic changes in the naso-~-pharyngeal epithelium (rhi- nocytogram and pharyngocytogram), by inclusions,: elementary bodies and finally by identification of the influenza virus, ixr"various pathologic products (naso-.pharyngeal washings., _Vates, 'blood, Scerebrospinal fl td, urine, and fragments of, viscera in lethal cases) and their inoculation to sensitive animals (mice, ferrets); Specific antibodies are revealed by hemagglutinoinhibition, sero.aeu 9l ~t bz#s- a Anplement .fixations, and precipitation on llc9~~ T~tc &p The nonspecific elements of diagnosis are: the leuko- gram, sedimentation rate, cold agglutination and certain me-c tabolic disturbances that appear in the course of influenza- The above procedures are described in detail, special stress being laid on the value of repeated hemagglutinoin- hibition, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 - 228 - 374.- M.VOICULESCU, MaRADULESCU, P.MICHEL, I-.SAMUEL. The incidence of influenza etiology in atypical pneu- monias. Second 1 oiahtific Session of the Medico- Pharmaceutical Institute,BDabarest, April 1956, 184. In the absence of influenza epidemics, investigations were carried out over a period of 8 months, on the inciden- ce of influenza etiology in atypical pneumonias - and a proportion of 29.1 % influenza pneumonias, was found, iden tified by means of the Hirst test. The clinical forms were medium and slight. The data obtained demonstrate maintainance of influenza in'the inter- epidemic periods. 375.- A.B$QN TKI, A, 'ET.REEEffi, PPETRP:SCUU, P,ATHANASIU- STROESCU'.-. Isolation- of an - influenza- strain with pre- dominant netirotropie "' character - from.- an- itfaiitiX lethal c e; ". St: eetc e, ihfra ni crobiol.: " mierobiol s .. parazitol 1957, 8, 4, 561. A new influenza virus strain was isolated from a su- praacute formcof influenza, ending in death of the infant, and adapted to the white mouse by intracerebral and intra- pulmonary inoculations of trachea and intestine. The presen- ce of the influenza virus was initially demonstrated by a positive serologic hemagglutinatio:,ition reaction, with serum standard A' from the pathologic products (intestine, trachea), taken from this case. The immune sera prepared on rats'and rabbits confirmed the antigenic character of strain A'. During passages by different routes (lung, brain) the strain exhibited predominant neurotropic properties, which were constantly maintained during the experiments. These pro-. perties were revealed by a shorter period of adaptation, ra=pid death of the inoculated mice, a more constant positive Hirst reaction and in higher titers for the strain main- tained by cerebral route as compared to the strain maintai- ned by pulmonary route. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Theanatomo-pathologic lesions caused by the strain . i oculated by intracerebral and pulmonary rou. n under study,, to shove the presence of the same pathogen# agent, determi- ning influenza lesions with similar characters,iin which the vascular element is prevalent. The toxic effects strain brought about clinical manifestations (convulsions) and anatomo-pathologic manifestations characterized by by- peremia of the internal organs. 37th:- N:;CAJAL, t4.MANOLIU, N,4DRAGANESCU. Evolution of ex- 14 t lar ac- -- ' t t ? e rea , pe-e rim rital influ n nir o .,957~_f 9561 St?ce ~x__a r ex erimental influenza in p mice., prolongs the period of incubation of the disease and slightly bwers the mortality rate. nronhglaxis in. t}~? 377.- A.DEREVICI. Prenatal antiinfluenz Report .-. s vaccine. autecht -~ n R.P. Re carried Wit-With a rea R.at~t .a'Se sich~ofth'O -.InstitC t 8f- ISf robi.t-- logy of the R.P.R. Academy, - September rder to determine the best period of vaccination t o , n for pregnant women, with a view to protecting new-born in- fants against influenza, investations were carried out on the anti influenza HAI antibodies in 57 pregnant women, du- ring the last 2-3 months of pregnancy, vaccinated with an A nine=fold increase autochtonous anti-influenza ooovaccine.,, in the titer of these antibodies is observed two weeks after vaccination; two months after vaccination, the titers begin to decrease, presenting only sixfold values and returning almost to the initial titer after about 3 1/2 monthmS. In the group of pregnant women which had rece3lMd apprQx ately 2 values months previously tetanie anatoxin,, higher antibody were obtained than in the women vaccinated only against in- fluenza, As regards the correlation between, maternal antibodies and those of r the new-born infants, examined.. in threw` diffe- ent groups, according to the interval between vaecination and parturition, it results that for each .group a fairly Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 - - - 23o close coincidence. exists between the titers-of the mater a1 antibodies and those of the child, It likewise resulted that the best period of vaccination for pregnant women, with a view to protecting the.child, is approximately 1o +5 days before parturiton, 378.x- L?DEREVICI, A?BRONIT$I,. A?PETRESCU. ~_nvesti ations on - the robl-em- of influenza virus caff`"Rep read at the Session of the Institate,_?f_,infraiorobio- logy of the RbP. R. Academy,-April 29, 1957. In view of isolating the influenza virus in non-epide- mic periods, hemagglutination tests were performed with the nano--pharyngeal washings of two. groups of ,without respiratory manifestations. persons, with or In the first eategory ewithout respiratory manifestatie ons ,. which included 12o persons, hemagglutinatioza was nega tive in the naso-pharyngeal washings examined directly; 8i- milar results were obtained with the allantoic fluids from eggs inoculated with these washings, submitted to concentra- tion with human red blood cells (3-5 passages), In the second group of patients with resPirato - ry manifestations forming par, of various collectivities with a total of 1,9oo persons, only 3 positive hem;a lutinations were obtained with the naso- pharyngeal wa'shi,so Inoculation to embryonated eggs of some"of the naso- phaXyngeal washings yielded negative hemaglutination reac- tions, Serum hemsgglutinating antibodies were positive in 8 patients with respiratory manifestations;* dynamic increase was observed (3-4 fold) in three of these patients, which made it possible to establish the influenza etiology although it was not possible to isolate the influenza virus. g It is probable that the vixM, from the naso-pharyngeal washings is masked during the mon epic to periods or that the phenomenon of reciprocal Interferenoo intervenes. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 231 3790- A?DEREVICI, AL0BRONITKIo Application of a cytol a i 1 the method fnr the rapid diagnosis of influenz 9 errors applied the Cateigne cytologic method in ct idemic in the RAP?R Report react at, .4 0 the Session of'the Institute of Inframicrobiology of the R. P. R o Ac ademy, July 18,, 19570 ~.~ The a the examination of tracheal washings in mice inoculated with the products from persons suspect of influenzae The cytologic aspects, specific of influenza, found in the cells of the respiratory tract of the mice, 48-72 hours after infection, make it possible to extend this simple and rapid method to the speciality laboratories in view of ob- taining an accurate and swift diagnosis of influenzae I t,PETRESCU9 AoBRONITKIfl DQSARATEANU, N. 3004 A~AEREVIG9 e DRAGANESCUo Comparative stud of the d namics of se- r,?~a are?i~~?.nflue A-Af Trod es &td" of. .nfluerLza strains . _ _ 6t. ceros , a~xifnainidro'oiol,o g icrob Ol parazitola, 19579 The titer of serum HAI antibodies was investigated at three months interval, by means of survey testse Comparison of the mean values showed that the curves of antibodies A obtained in 195`5?- 1955 showed a disjunction between the curves of antibodies A' ,which 'ran parallel durng the two years of investigation, and the curves of antibodies B. which ran, parallel at the beginning, but showed a marked. 1()55, partie*a- to rise during the last quarter of ency tendlarly in the towns close to the USSR, where as influenza c type B epidemic was reported. A comparative study of the strains isolated during twa years likewise showed that there exists no diffe- virzlenoe ~,_. between them as regards ad.aptabilit for the number of pathologic prodizt isolated was however greater in 1955. The isolated strains belonged to t5 vpe AY, having also antigenic AO f ormati with the excepa- `iJ of one strain type A? which exhibits4 prevalent near. +:ropic plopertieso Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 -232- 381.- A..DEREVICI, A?PETRESCU, A.BRONITKI.. Variability in nature of the influenza virus strains studied in the R..P..R,e, in the 1953 ? 1955 period. St.cerc.inframicro? biol., microbiol., para2it6is-l957, 8, 2, 173- A comparative study of the influenza virus strains isolated in the R?P.R. in 1953, 1954, 1955 and these strains and the standard A/PR8, A'/FM 1 and B/Lee strains was car- ried out. The strains present a complex antigenic structure be- longing prevalently to type A. but having also fractions A' and B, more accentuated in the group of strains iaolated in 1954. The biologic properties (adaptability to mice, virulen- ce, interval of time until death of the animal supervenes) were more evident in the strains isolated during the epidemic period, as compared to those isolated during the inter~epidem mic period. Investigation of the evolution (every 3 months) of the serum HAI antibody titers showed that this titer, which re- flects the anti-influenza reactor rate of the population, was modified paralla,. to variation of the antigenic structure of the isolated strain. 3820.- A.DEREVICI, A.EETRESCU, A.BRONITKI, D.SARATEANU., Biolo- gic characteristics of the influenza virus straihb isolated in the RoP. R.. in 1956 St. sere ? iiifra~nioro- biol?, microbiole,"paraLitol~ 1957, 8, 3, 34.9E The biologic properties of the influenza virus strains isolated in the R.P.R. in 1956, are investigated in comparison with those found in the previous years, showing that from the points of view of adaptability, induced morbidity, viru- lence, HA potential and induced histopathologic lesions, the ,strains isolated in 1956 resemble those isolated in non-epi- demic periods and differ from the strains isolated in the epidemic period by a decrease of the biologic properties in- vestigatedm Dynamics of the serum HAI antibodies chow an increase Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 233 for type A. corresponding to type A of the strains isolated in fieldwork, which demonstrates that immunization of the pop. ation takes place especially against the predominant strain. Antibodies A? give very low indexes, reflecting the disappearance of the A' fraction from the antigenic complex of the previously isolated strains The reactor rate to virus B shows a significant increase, more'evident than in 1955, particularly in the towns in the North--Last of the country, close to the USSR, in which type B epidemics were reported. 383,x- A.DEREVICI, D.S:RATEANU, AoPETRESCU, A?aBRONITKI? Laboratory investigations on the 1957 ZOfluenza epide? mic - in the R.P.R-?, tt., cere. inframicrobiol.?, microbiol?, parazitolos l957, 8, 4, 507? During the summer influenza epidemic, a series of strains belonging to type A, having antigenic fractions common with the strains isolated in the R,.P?R. during the 1953-1954 epide- mic and with strain A (Asia) 1957 were isolated on embryonated eggs. These investigations showed the absence of a good a.dap- tation'of the isolated strains to mice, it being necessary to passage the mouse products on embryonated eggs in order to establish the presence of the virus.. Serologic investigations in patients during the acute phase of the disease and during convalescence revealed a dy- namic increase of serum HAI antibodies, especially for the A/PR8 strain, as compared to the 1957 strain (Asia), The leukocyte counts performed in the same patients during'the acute phase, showed a smaller number of *hite blood cells (an average 5,o69 elements/cmm) than during con- yal_e_sc&poe (an average 9 elements/cmm) and an increase of %. of 22 - 44 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 and staphilococcus),, by intr4.asai or intracerebral route,, in various dilutions,oti -Hemorrhagic and, degenerative lesions were frequently tbsued in the toxic forms of influenza in mice and baby- mice B, inoculated wi~h large doses of influenza virus,, whe- reas the inflammatory reactions (perivascular infiltrationg' gliesis) were.observed in the purely nervous forms,, in the forms associated to pulmonary lesions and in mice inoculated with the influenza virus associated to bacteria4 Perivasou- lar-and peri:cgllular oedema was observed especially in baby- mice?. The.path?genic mechanism of nervous -manifestations in most` influenza cases in breast-4fed and_..small. infants is an- counted for by the direct toxic *Si.q~% of the influenza vi- rus upon the capillary endothelium at first, and then upon the nervous cell; anoxemia following vaaeul r disturbances contributes to the seconda nervous sgns Multiplication of the-influenza-virus in the nervous gel1 or actual neuro- tropism has not yet been demonstrated, although it scams possible.0 path.bgenic,bacteria (haemophiius influenzae, pneumococc 234 o 3840- GJMARINESCU? On meningoencephalitis of influenza origin-. and experimental investi- gations'% The Third Congress of Clinical Biology, Bruxelles, July ]A--2ool9510 An anatomo-pathologic study of the central nervous system was carried out on 25 breast-fed and small infants with nervous manifestations,,, that died of various forms of .nfluenza,,and on 232 mice ands rats of various ages, inocu- lated with a pne.umotropj~o~ influenza virus on the one hand,, and on the other with the sam.e virus associated to certain Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 ? 235 - 3854- I;.MIRZA EMINET, A.PICOS, AL.PETRESCU, AL.BRONITXI. The role of cortical reactivity in the course of anti- influenza immunization in white mice:., St:.cerc.:inframi- crobiol,... picrobiol.?a parazitol*,. 1957, 8,, 2,. 191.. Investigations were carried out on a group of mice, in whom motor-alimentary conditioned reflexes had been elabora- ted., This group was subsequently vaccinated with the anti - influ6nza vaccine, After vaccination#~ modification of the conditioned reflex activity was observed, manifested by peri*__~. pdi.t alternation of predominantly a Cation phases and pr-ea. doninanti.y inhibition phases. No modifications appeared i* the controls., These disturbances were slighter than those observed in previous investigations, in which the animals had been ino- culated with non-attenuated influenza virus.. It is concluded that the law of intensity known in phydiology'is also appli-. cable in immunology. The above mentioned disturbances appear sooner in animals with a lively temperament, than in those, with a phlegmatic character, in which they appear later. 386.I. MIRZA B . P..ATHANASIU-STROESCU t AL.PETREScU, AL. BRONITTI a 8F.~ Stu of anti-influenza immunogenesis in white 'rats *1tt -_varlo s 0-1 _. of - ziervou19 - 3 stem carried out. 1:utifri6ti6t i jbition test and, the- m~ `hO Of nditi d refle es Stcerc. infra i biol?Dmicrobiol... parazitolH., 1957;'8,:4.,587. Rev..ScL Med. B 19598 4 , 61.. A study was carried out on thereactivity of an*U in connection with the nervous system type., A number 0*awni- malsq, in whom motor --alimentary conditioned reflexes ha been elaborated, were inoculated with the anti-influenza vaceine or with the influenza virus, Inoculation of the vaccine or of the active virus causes an'accentuation of the inhibition proces , more intense and persistant in the animals of phleg- matic type, more discrete or of shorter duration in animals of the choleric type. After vaccination, hemagglutination-inhibiting antibo- dies do not appear in animals of the phlegmatic type and are Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 236 to be..found in low titers . however,; in the animals of chole- ric typez. "fter`noculaton withrf the 'active`-virus's the titer is somewhat f -increased, but ithe s"ame ratio is maintained bet- weep the choleric rand the phlegmatic} t' es., yP _ Booster doses', erformed 11 =weeks later,;, wipe ,out these fferences between. the two diff t eren . types: of `the ne;rv:ous iysteino, 387. D.SARATEANU, B;*FUHRER. Study of anti=influenza immuni ty in .p`revi Duals a iti-?vaccinYa immunized animalso cerc.. inframicrob1.0 ' microbiol P.- 2o7... t ~ k 4 Report read at the Session of the I aatjtute o ~ ani a obi olo ~ of the R.P.R. Aea .emy on August 117 BW ana117 . Kie amt L enio relations betweer, the influem, r ai.x s i3olated im, the 1959 epidemic and the strains i.m t6iO proooding yearoq It was possible to establish Qte.3t to sx?e ip characteriaties were *aiatai ned 4~ o ar . tho vaxNiability that ouperwener with time. zoo anal%L of the structure of t ,e Asians A2 stra.na .. i 1951 and those isolated the 1959 epidemic made .. ~ponolbl - to- we them a@ va oe1aation strains 0 since thoy exhii a ou~~r a tiffesaie potentials ?._ appearax of xod A + A2 type of strains suggests {'~.c e o~ to a 2zi mat e of both type@ of virus ? A and A29 "; :aC~ ~c~ t inn i oa leadi,AG to the forsatLe of newt a? of AoM. Mt3'Xq Mo ON I0 ALo $'CUa ! J? ~ ;p! l_ 'tea 9 3c~ y~c n _f~ __ go V?,o M cripa 0 - Data a supra n p .6,nei 1i 1959h, T ado 1959a 82 influe sSa virus otr .ng isolated. in the 1959 opido- , to the .utoe tomeus strains isolated in >~~~~lq aye r~~hoaoc~u~q their antigenic structure Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 ? 251 - allowing their classification as strains type A/PR8, type A2 and mixed strains A + A2., The variability^of.the strains isolated during these years is shown and demonstrated by crossed hemagglutinati on- inhibition tests. The constant changes.in the titers of serum. hemagglu-_. . tinoinhibiting antibodies are likewise shownsp a U+ aq;-?de- Qj-z;;&;Cp anti-A2 HAI antibodies before the beginning of the 1959 epidemic. 427.- A.DEREVICI, AL,BRONITKI, AL,.PETRESCU acid C,2ATMARI Preventive action of ' the Rumanian anti-winflueizza va= Gripa-"-Date-aetlptA_epideffiiei-din, 1959, EUMed; anw.1959; 161. The prophylactic activity by means of anti-influenza vaccination started in the R.P.R. in 1954, when experiments: were carried out with the anti-influenza vaccine prepared from mouse lung. The prophylactic section of the Institute of Inframicrobiology subsequently prepared a purified ovo-- vaccine, with the aid of a special apparatus,. built for closed circuit handling, which reduces the time of prepara- tion of the vaccine, administered by intranasal route by means of a sprayer with which the amount introduced can be measured. Application on a wide scale of the vaccine in 1959 was preceded by various restricted vaccinations during 1956, 1957 and 1958. It was observed that the vaccine prepared with strains type A*PR8 likewise gave protection against the pandemic vi- rus A2/1957. In the course of the 1959 epidemic, in a group of 3,114 children and 1,907 adults the total average of the protection index was 5*93 when the vaccine was prepared with the same type A2 strain, isolated however in the 1957 epide- mic- ? The vaccine applied in the 1959 epidemic acted by in- terference Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 i ?259? 4280- A?DEREVICI, N,DRAGANESCU. Cultivation of the influenza virus in vitro in a fluid medium: The action of added thymo and ribonucleic acidaComunicarile Acad.R.P.R.p1959e Cultivation of the influenza virus in vitro in a69 e39. fluid medium according to the Lee and Rivers method, was performed with certain modifications consisting in the ad- ding of thym, and ribonucleic acids. After establishing the best concentration of embryo tissue in Thyrode solution,. the effect of various nucleic acid concentrations was studied. A delay in growth of the virus was observed follo- wing the additionoof thymonucleic acid and an inhibitory action in the case of ribonucleic acid. These results are attributed to the action on the embryo cells and not di- rectly upon the viruses. 4290- AoDEREVICI, ALOPETRESCU, P.ATHANASIU. Experimental sty z,.dy on _the..:Lmmunogenicity of the, inflaenza-viru.s' strains isolated in the R.P.R, in the epidemic '.and Postepidemic,e ,dS "."'Mierobiol:s pd!,- at It 61'e"11 epid.eniol. , - i959,.-48 4o5?409. The imrpunogenetic relation between the Asiatic t; e ': 2) of influenza virus strain, which caused to i our country, and the influenza virus type A/RRf ?i,~ o.Lat.od. in the postepidemic period in 1958 is it N? C) Tfhe r c f: ~> int; .u.n:,ity tests carried out on mice showed protection, which does not correspond o: some authors in connection with the to man by vaccines prepared with ,: -'ainst infection with the A2 epidemic The expe'o.!.v~.ts ca tried out on mice demonstrate the advantage of *,-, Lactic acid evaporated 6o mi- nutes before aerosol .spraying of the influenza virus as well as after aerosol spraying had no virucide effect, Still better results were obtained with fumigated fir- vruL resin, (alcoholic extract of Pinus alba resin),, ? Fumigation carried out both 30 minutes and 66 minutes before aerosol spraying of the influenza, virus, led to survi- v of loo % of the animals. The fluid alcoholic extract of 1inus albs resin did not have a virucide action. b`5oe= G.MARINESCU . A simple procedure for investigating co1._,. agglutinins: a " gt idin9" test- ii the dia nosis of iflf. a ii- erobial primate atypical" pneumofiiaa Sts ? ces~co "'infraicrcm biol0, microbiolo pa:ica ito1;; 1951, 2, 1-2, 1570 With a view to-investigating cold agglutinins for the diagnosis of primary viral penumonia, a simple, economic r-ethod is described which can be applied at all ages (especially breast,-i,fed infants0 This method consists in drawing the blood into the Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 4w 272 .. white blood call pipette up to the o.15 mark and diluting it wha.tb. saline up to the -11 mark} the oentent is put into hemolysis tube and then into the iie-box, it 6 be read after 4..18 hours. The agglutination titer, when agglutina-_ i=m ocours, is approximately 1/125, the lowest limit at which such a reaction can be considered positive for infra- microbial atypical? pneumonia. If the reaction is negative, can bit readily repeated, at momenta particularly in the mouse,, certain of the aspects not- in 3umah pathology. severe (mortals ... Y ty rate 154 %) especially in .infant inoculation to the common laboratory animals induced, =he total . number' of pneumonia cases observed.,,, was excepti?- Primary viral pneumonia,. representing about 10 % of trim; 195, 2 ~2 ... 51:. LLVOICUU SCU, G. Ii CU. C;i 1 ?and:e.., er# ienta1 im~res ati ons o Vi al a c~ni 14,ren. Podia- 452,'_1 .VOICVLESCU, G, INESCU,. Pr mar$ Viral_..x~neum in children., Clinic ,,__ ?~? ai, mor~ho~athologic and::exr~ r meht.al to o $to -dertd.. Pediatric ,. 19 ll` Cl .~;al a n d i abser? at ion in hivzan see Ire Pressed to ge t ier . %N t .. a comparative study on :Laboratory animals in which some of the hi rtopathologic as.. ecte encountered in man were reproduced by inoculation of pathogenic products. The clinical study of 26 cases of pri?- -. ry atypical viral pnnumonia in children (6 months to 16 years), representing to % of the total number of cases of pneumonia hospitalized, revealed the severity of this di- sease in small infrn s in which the mortality rate attained 25 % due to nervous, complications, The morphopathologic examinations showed at the level of the' lung interstitial.infiltration with prevalent mono- nualeara, vasoUlar changes that led to disturbances of res= piration and degenerative.-proliferative leaioaa im the- - Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 ice, a u !. !r&SM p ", g i 7) r.Ie}! -virsl L ,; brain) from 2 lethal r .. xa,gs p:et`'v severe route. of i ffia ri o } u;e-, on ,a0 showed that the most respiratory routefl and the most viralen . ? _ ,, II matous degenerat? broncho,vascular t xl o Discrete par xioh system were obs? Lions and modifications ~f the s e~enou m ? stems ~ ningo a the new In ve in the other organso The "?an.i.mai,G inoculate ~ml, ijnfiltrations were rt~tc do ,- tho eni . pro tof 273? ? hR! lul: gs and spy e A0 affected organs..?were:, the i s ere .Y The most sev mesodermal componen of the nervous y stem and t Gib ?? ? ar? _b d newo - rec the live an,, l.e er d"Ag (I ki 3'all A Ti r l Es E o 'A y MoM.IRSA I9 SoGIR ARJs. d gy a T l CU R S `,fi9 ?o ;E3A~tASIST.a L'1~.Ab the CtR ~ ; ' aw e S p u oft t- .,, 9 R~ 6" if?fT,t7 f d opid ffii o B liar St 4 5 19 8 S ^ases of a,o?,te peaania we adm 7L,(, ~ ?' t. o lesionat the ba pneumonia lesicrss o eri.co wpF~al r mixed were constantly ne~sQ g nth n?eoropti oases t: , , The a i logi? tud 's on the other hands revealed Lin ,ra ^= lases a non-hema2utinatzag inf ramirob3:a1 afagaui; path g 6 n i ~ . for mioee These animals died i &5 days or days after peritoneal ino ^ .a i.on by intranasal route or A t ino: ulatio -:o The virus passes L'"~Ugh the Seitz E,93 filters ra maintaineu~ in saline at 2o~'Q r i glycerin ~t x'40 in neutralized b the arati~influea A8 9 A20 BO C and ~^a and. dove not pros immunize with afty of the influe ~ Thy histDlogi,,e lesions were eithea; p-. in 1 ~L sad t the .5, oond -Clinic of pediatrics, Be har?sts of Which 2 wer r diagnose ~ as atypical pneumonia o three of thec tomatolo= eases alone. s u pi? eaio In view of the clinical syp " hi.st .1ogi@ lesions * and serologic reactions, all the ca- erp listed in the group of primary atypical p ,eumoni.a taJYe~ ) and influenza atypical pneumonia (1 ease) ? In. 2/5 of the cases the evolution is supraaaOut f 01- Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 274 As T za virus,-)s isolated in our country, pathogenic for mice. It is not inactivated by penicillin, streptomycin and ter. ramyci.n. 454?_ V.PANCU, AL.,PETRESCU, P.ATHANASIU, Considerations on a viral respirator epidemic in the s rin -of 1 8-s in the town and district of Boto ani o-'Symposiur-on the _' 1957?1958 _ ifAflu?S2a - epide io?"Bacharest, December 4-5 1958. In the March-June 1958 interval, 367 cases of acute r~ -piratory affections were reported among all infants, taking on the aspect of an epidemic outbreak. Of these ca- ses, 166 were diagnosed clinically and radiologically as .ypical broncho:+pneumoniat with a severe evolution and a :or cent mortality rate. The onset was usually insidious, niter 3-4 days the symptoms became more se1rnre and the ge- noral state developed towards collapse accompanied by the other characteristic e'_gns of broncho-pneumonia. The attem S ; made at isolating the causal agent from the naso--pharyngeal secretion or the .-Fziezes revealed in 2 cases a non-hemagglutinating virus, pathogenic for the white mouse by intraasal or intraperitoneal inoculation. The animals died 14-15 days after intranasal inoculation ,id 4-5 days after intraperitoneal inoculation. The virus passes through the Seitz EK.3 filter, and is stored in -,.yeerin at + 4?C or in saline at - 2ooC. It is not neutra- lized by anti-influenza sera type A, Al, A29 B, 0 and D. w' -d does not cross-immunize with any of the other influer - za viruses isolated in our countrye The virus is pantropic and can be revealed in all the organs of animals which died of the experimental disease. It is not inactivated by antibiotics, penicillin, strepto- mycin and terramycin. FOP OFFICIAL USE O Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 - 275 - 455.- D.;;ARATEANtN.DRAGAN'"('TTY EL.NASTAC, EL.OPRESCU, in -bulmonary_ s:^Conferenc~ f_th.e-CzecYiosib~'a8fan viral affection virologists, Smolnice, 14-17 October 1958. A serologic study was carried out in 130 cases of primary atypical pneumonia. The results obtained show that influenza viruses of viral type A and A' are incriminated in the etiology neumonias in 42*3 % of the cases, the ornithosis virus in p 36,9 %, nea-rickettsia in 6?1 %, primary atypical pneumo- nia virus in 2.3 io and iymphocyte choriomeningitis in o-.76 % of the cases. Attention is drawn to participation of nee-rickettsi- as in the etiology of atypical pneumoniasi and stress is tests laid on the importance of double and triple serum q in order to be able to make the serologic diagnosis* 4569- D.SARATEANU, N,DRAGANESCU? Non-influenza viral InO & ,; AAm3 n-Re-Dort neumvula~~~~_. - - ~0ember 195 read at the , Inf liUebta - Sy~po sit ., `4" 5-Bucharest. Experimental and serologic. irestigations on the are described. problem of the etiology of viral At the same time, the data in literature, refering to the etiology of viral pneumonias are reviewed. From the data presented, the possibility of esta- blishing the etiologic diagnosis by means of serologic reactions, isolation and identification of the virus re-- sults? Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Mixoviruses 276 - 4 D.SARATEANU,. AL.BRONITKI, Laboratory diagnosis of in- fluenza . Microbiol. , ' parazitol. , .. epidemic ?_P 1959i- 3.251* The paper is a synthesis of the diagnostic methods-ap- plied in influenza. The dynamics of the data are shown and mention is made of the products to be collected from patients and neoroptic cases, of how to prepare the products for ino- culation, inoculation of sensitive animals, embryonated chick eggs and tissue cultures. The use of the serologic (comple- ment fixation, hemagglutintLtion and hemagglutination- nh3bi- tion), cytologic (Koleaditzkaia and Panthier) and histologic methods of diagnosis are. described. 4:8.- R.PORTOCALA, V.BOERU, I.&AMUEL. The...role of..ribonu - cleic' acid in the infectivity' of O.Sfiflueh ,a'yii^us. St.cerc.inf'ramicrobIol; i ,1961! ?l -1-#%i;...,....,-.,,...M., The phenol extract obtained from the influenza virus contains ribonucleic acid that is the infectious factor of the virus since, inocu-lated into the allantoic cavity of the embryonated egg it induces the sensiti~vf, cells to syntheti- ze the 'homologous virus with all its morphologic, antigenic and infectious properties. The ribonucleic molecules are very labile and under t action of ribonuclease, temperature and prolonged storm .e, completely lose their activity. The new influenza virus strains produced by intracel- Wlar synthesis under the action of ribonucleic acid, pre- sent certain biologic characters that differentiate them from the initial strains. The virus strains of antigenic A2 type never release active' ribonucleic acid, probably due to a high proportion of incomplete elementary bodies. - Recently isolated type A virus strains appear to re- lease a much more active ribonucleic acid them the strains carried through numerous passages. in the embryonated egg. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06 CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 277 ? Synthesis of a virus prom its ribonucleic acid may be obtained not only with simple nucleoprotein viruses, but also with more complex viruses, such as influenza virus. 459.0? AroDEREVICI, AL?BRONITKIe Study of tissular antiinflu.er.- za antibodies iu animals app' ently?resistant~tO tbe_~ experimental infedtio.L,, cer inframieroBio ;; -fi%6o9 .11 1 During.investigations on the immunogenesis in influenza tissuiar..and.serum antibodies were investigated in animals apparently resistant _...:-uenza (rats and rabbits), subjec- ted to repeated bleeding and perfusions. Separation-of tissu- lar from serum antibodies is best obtained by the technique proposed. by the authors, i.e. collection of organ fragments before and sifter perfusion. The serum antibody titers warn higher than tissular titers. ' Tissular antibody titers, before and after bleeding, present differences which are more accent -: tuated in the spleen and liver, organs with an ample circu- la.tion,e The highest tissular antibody titers were found the lung' ' a fact explained by the pneumotropism of the virus; then the kidney, through which the virus is eliminated. The ,. liver and spleen present low titers. 4- P,ATHANASIU 460 AL.?PETRESCtJ. The effect of cultivation of , , the' influenza irus* straif-A1' /53+1 ^ahimafis"'~v3tfi"ID~O dified readt y;- St e~daiSff~midFSbi01 I9 O 1- ll7.--WT,ifobt,;Bled. Roumm 1960, 12. An influenza virus strain adopted to the chick embryo and slightly pathogenic for the white mouse was cultivated in normal mice and in mice previously infected with 1/2 LD50of street rabies, herpes or poliomyelitis viruses, or immaftized against rabies or poliomyelitis. The variants obtained were. studied as regards pathogeni- city,, hemagglutination, antigenicity and immunogenicity. The values of the biologic properties of the variant cultivated in the rabid mice were close to those of the initial strain Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 - 278 - with an increase of pathogenicity and antigenicity. The biologic properties of the variants cultivated in the ani.- mals previously infected with herpes or poliomyelitis, or immunized against rabies or poliomyelitis, showed a decrea- se of the values as compared to the initial strains, except for the immunogenic potential which was higher. With the virus cultivated in normal.mice, the pathogenic potential, hemagglutination and antigenicity fell to zero, and only the immunogenic potential was maintained, at a lower level. 461.- A..DEREVICI, AL.BRONITKI, AL.PETRESCUQ Biologic aspect of-influenza virus strains. isolated in-the Raman1ah- Pe6fi1a's_Rep blie, Relationahi$,t the Pebraar~ -"" MBtch'epidaffiid-sttaies;-St: eP ;-inrmm1erobial. A comparison study was carried out on the influenza virus strains isolated in the country from 1953 to 1959; showing the variability of the autochtonous strains and` the appearance of new types (A2) . Study of the influenza -vi us strains isolated in the country during the 1959 February epidemic showed a lack of homogenicity; during the first days an :a~uf]:ueiza.lstrain t3 p; A (PR 8) was isolated, then type -122+4,4 finallyA+A2. The strains were isolated during-the-epidemic period in various university centers in the country. Y.COPELOVICI, M.GRUTA. Experimental investigations on influenza virus - straing-igolate -drLtSng"t$?""^^ 1959 _ gebr~ cry-M~trc$~eldemio:St oeo i!remierobioi. Study of the pathogenicity for mice of some influen- za A2 and mixed A2 + A strains, isolated during the 1959 February-March epidemic, showed a low pathogenicity for this animal. The mixed virus strains can be adapted to mice after previous X-ray irradiation of the animals (45o r). Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 - 279 463.- E.SORU, A.DER1CI, AL.BRONITKI. Chromatographic determinations of ' ce- -- ~;. amifoacids: frog influenza virus ains isolated " in the Rumanian People's Repu- bl during the 1959 e ?idemic Sts care e itf r idtobi(l. 196o, 11, ... 3 ..357 ? _ _ .. Chromatographic analysis of certain inoacids from influenza viruses isolated during the 195$ a uid:eme (Al, A2 and mixed A+A2 strains) and a physical mixture in egt.al parts of A and A2''eluates, showed: 1. More significant gt.antitative differences as regards tirosine', glutamio acid, argiuine and the aspartic, serino and glycocol grc,s.p; iidl imal differences were found for leu- cine, alanine,.phenyl.-ramylalanine and no differences for valine and lysine. 2. Comparison of aminoacid concentrations in t the mi- xed strain and the physical mixture of the component A/59 A2/59 strains, showed equal values in 66 % of the aminoacids determined. 3. Comparison of aminoacid concentrations in strains A2/59 .and strain A2 isolated during the 1957 epidemic, sho- wed different values for glutamic acid and lysine and small r1 "fferences as regards the concentration of the other ac ids. These results characterize the correlation between the chemical substrate and biologic properties of the various strains investigated. 464.- A.DEREVICII L.PBEDESCU, AL.BROITITKI, I.COTARGEA? 1'articul itie f '_.. fl x aza. ti.'..:. strain of. the t Sate - t . . r -1959 . hiic dur mitred A+A 1 poi eted" fa" Re-- S cerc. ramier-a'bi o1-ar I96o, -11w ;3~r 375. Vi.~ ologic study of strain Pa/ '; iso1et+ towards the end of 1959, showed in inoculations to mice predominant neu- rotropism. Intracerebral inoculations permitted.iieolation of the virus from the first passages. Adaptation of the strain to the ' lung is obtained only with products from passages in the brain or the allantoic Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 28o fluid of embryonated eggs, likewise after the first passa- ges. These characters differ from those of the mixed A+A2 straiiz isolated at the end of the 1959 epidemic, -probably due to a new constitutional structure of the virus, which underwent, during the 7 months interval,, numerous passages through the human organism* The hyperergic character of the;d.cliAie 1,.&volutioa in children may be=attributed to the bi-ologic characteris- ties .of virus Pa/59, as , well. as to the_ specific reactivity infants, ,- A.DEREVICI. Heterogeneity of certain influenza vixus variants tested _.b_ th? - techni .. to of end infectious dilutions: F,t:oerc; i8fi's i robiol:, ..196o ;ii;4;55% Investigations were carried out by the technique of ;nd infectious dilutions on some influenza virus variants isolated during the 1959 epidemic, presenting from the se- rologic viewpoint the com.:lex antigenic structure of type AandA2.' .Determinations were. done with 1,75 % and.33 % of ID50 and seroneuitralization with immune serum A and A2. The strains obtained, were..1 hibited by both sera A and A2 &n d others only. by~ A2o t . of_ type A2, resulting from inoculation.of 3 % of ID50, Neutralized by antiserum A J o f , strains A2 and A+A2.The ,perimezrtel ;results : showed that the strain investigated 1 a transition strain, with an instable structure, that Y be dissociated--.in the course ' of the passageso 'I).MIF1ROIU, P.ATHANASIU,. Y.STRULOVICI M.POPA. The varied, etiology of certain influenza-like cases with an epidemic character -Report--read_at-the"session'of the-Institute"'- of Infraticrobiology, July-4. 1960. In order to establish the etiology of certain influen- *.A1ke cases with an epidemic character,.investigations were carried out on 25 of'17o cases diagnosed clinically as in- fluenza or influenza with pneumonia. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 281 Cytopathic agents were isolated in tissue cultures from 8 cases and identified a:~ i orming r art of the APC group by complement fixation. A virus, isolated in mice, was neutralized by conva- lescent serum and Boto-antiserum. Attempts at isolation of the influenza virus remained negative. The predominant adenoviral etiology of these cases became apparent and attention is drawn to the existence of epidemic cases caused by APC viruses and the necessity of bearing in mind this possibility in future etiologic inves- tigations 457 $.PCRTOCALA, S.DUMITRESCU9 N.IONESCU, I.SAMUEL9 V,BOERU. Replication of the influenza virus y mean ICI Morpholo$id as eat of viral ri.b?nuclei acid, .-- ofthe generated ~rizsusm"Courtin: Aead.R.P.R. 196O; A study was carried out in the electron microscope on the morphology of the influenza virus generated by the ribonucleic acid extracted from a strain of the pure A an- tigenic type. The morphologic aspect and the size of the viral elementary bodies obtained in this way do not differ as a rule from those of the initial strain. Thus,, the mean size of the Virus from the first culture and' from the first passage in embryonated eggs is leoW7 ? o.7 m1 ? This is identical to the size of the initial virus,, .from" which the ribonucleic acid was extracted. Serologic investigations demonstrate the antigenic identity of the ribonucleic acid generated strain and the initial strain. The morphologic results obtained eonflxm the hypothe sis?'a(ftording to which the receptive dell syn- thetizes the @"V'Iete influenza virus starting from a tem- plate of the nucleic acid molecules introduced into the cello Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 2.82 468.- R.PORTOCALA, S.DUMITRESCU, N.I.IONESCU, AL..B.RONITXI Morphologic aspects of an influenza virus strain of the"mixed. A + AP " type as compared to types A and A2. ....... Rev. Sci. Med.196O,,2' 3-a-49.2230 In view of the experimental results obtained, the authors consider the mixed influenza virus strain as a physical mix- ture of two different populations, each belonging to a diffe- rent determinant type of influenza virus. 469.- R.PORTOCALA-'., V.BOERU, I.SAMUEL. Replication of the in- fluenza virus ,y means' of viral rib6 ucleie' acid;"'"' IV.- Study of . rib0Yitaeieaee 3.i"e~~ated ';agga` of va- ~iou.s Study of ribonuclease in chick embryonated eggs showed that in the chorio-allantoic membrane and the allantoic fluid, the enzymatic amounts follow an ascending evolutions. in terms of the development of the embryo. It is recommended to use, for investigations on the in- fectivity of influenza ribonucleic acid,, lo to 11 days old eggs, since they contain the lowest amount of RN-ase. The likelihood of certain quantitative and qualitative changes of RNA in terms of the season in which the virus is cultivated in eggs and, implicitly, of nucleic acid extrac- tion, should be taken into consideration. These changes might explain, together with the evolution'of RN-ase, seaso- nal failure to produce the influenza virus from-its RNA. 470.- R.PORTOCALA, M.ANDREESCU . Replication of the influen- za virus by meafig"of viral ribonadleie" 46id.:V . IYifluen- __ rr r ce of the giial.ilF,ar~ of phenol on_ribonucl.ege acid"acts= ~I Awn ~~ vity. St cerc "infrsmicrobiolo, 196l; I;-77;- _ The impurities found in the four types of phenol sup- plied from different sources and used for the extraction of viral RNA, were identified and determined. The presence of heavy metal ions, especially lead, in some phenols, accounts for the inactive RNA obtained.The smallest amounts of impu- Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 riti es are to be found in Soviet phenols with which an active RNA is constantly extracted. It is recommended, in order to prepare antiviral vacci- nes attenuated by phenolo to use a pure solution of this subs- tance so as to avoid total inactivation of the vaccine virus 471?. O.RURDUCEA0 MGCEPLEANU0 R.CAPRARUe- RKOlution_of ^HAl antibodies in white rats immunized a : aifi.-Winflflan.2as u.ndor the influence of "' l and- __ i..t ?adiatiOnB; " St. cer{ infraini.erbbio1 ... ,96i,~ "~ is 1370 Investigations were carried out on the evolution of HAI antibodies at 4. 8fl 12 and 2o day intervalso following ino=- culation to white rats immunized against influenza, 25 )C amounts of 1131 or P32 or stable iodine or phosphorus. at a pH and concentration equal to those of the radioactive solu- tions. It was found that under the action of gamma radiations emitted by I1310 the titer of antiinfluenza HAI antibodies significantly decrcasess as compared to the control group, P32 stable I and P do not influence the titer and, eve- 0 lution of antiinfluenza HAI antibodies. 4720? PoATHANASIU9 M.POPA? A study of hema_g l utino.i,ibiting influenza' antibodies ifk-rabbits' ihoc .latefl"wilt a"virus into the Cori ncti rai sack"afterlig~t, on-and sed-tion of"the" lachr maT cans:Co "St: "cerceinfrafnicr~b ? 19619.x,29 229m_"-.r--'- -- -_ Inoculation of influenza virus into the conjunctival sack of rabbits whose lachrymal canal had been ligated and sectioned, resulted in a moderate increase of influenza an- tibodies in the blood serum- This result points to the poa sible occurre e6~7 f influenza' infection by the conj tival route, i.e0 iA&SDVe ado -tly of the nasopharyngeal route, and confirms similar investigations reported by Cl.Hannoun. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 284 473.- ACAD.ST.S.NICOL U,. N.DRAGANESCU, V.BOERU, N.I.IONESCU_. Saectrophotometric investigations in UV light on_ribo-? nucleic acid extracted from the influenza virus inacti- vated by copper ions, St.cerc.inframicrobiol. 1961,126 3, 285; C.R.Acad.Sci.1961, 213, 1503- S I tudy of the absorption spectrum in UV light of tibo? nucleic acid extracted from the influenza virus inactivated.. with copper nitrate in. a 0.5% concentration did-not show any change in the ribonucleic acid curve. 474.? R.PORTOCALA, V.BOERU , S.$4RAGEA, IGSAMUEL. Reproduction of influenza virus with the aid of viral ribonucleic acid. St.cercfi framicrobiol. 19618 12 , 3, 303. The ether-phenoi&... axtract of influenza ribonucleic acid, subjected to dialysi? precipitation with alcohol and redisolved in normal saline, presents in ultraviolet light and. absorption curie characteristic of. ribonucleic acid, with a peak at 258 Up. The absence of proteins in the studied extracts proves. once more that the infectivity of these extracts is due sole- ly to ribonucleic acid. 475,-R.PORTOCAI,A, N.IsIONESCU,. S,DJ'MITRESCU, V.BOERIT, I.SA- MUEL, E,PACURARU.ReproductioU of the influenza virus with the aid of viral ribonuc,leis acid. VII.- Morpholo- gic aspect of ribonucleic acid, St, cerce inframicrobiol. 1961, 12, 3, 3o9? A study was carried out in the electron microscope on the morphologic aspect of infective ribonucleic acid extrac- ted from influenza virus. For this study the preparations were made with the products collected begore the end of the extraction. The electronooptic examination showed the presence of characteristic strands, In most cases these formations are joined together along their lenOforming thick clusters. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 285 The diameter of the finest visible strand in the prepares- tions is 30 . . Certain hypotheses are emitted on the.- imp-possibility of evidencing finer strands. In the products collected at the end of extracton the strands described are no longer found, a fact discussed by the authors., On the same preparations, the presence of viral ele- mentary bodies, with a morphology deeply altered by the.. btutal action of phenol, may likewise be observed. Their presence may be accounted for by the fact that the ribonu- cleic acid was obtained from incomplete extractions, 476,? A.DEREVICI, G?BAI US, AL.BRONITSI, G.ISAIA._ Local-and Eeneral paraallergic phenomena obtained in the rabbit with .the influenza virus. .St, , ccre.. infremicrobiol. 19618 2n 3, 335; Rev.Sci.Med. 19619 3-4. The findings of the authors show that the influenza. virus may produce in the rabbit localized (Schwartzman)...or` generalized (Sanarelli-Schwartzman) pars-allergic phenomena. Leuko=and plateletpenia become evident 2 and 4 hours after the inducing injection, followed by hyperleukoeytosis and return to normal of the platelets. Quantitative differences are obse:rve.d...in..the.._.contrals...._..._ The same hematologic characteristics were found. in the group subjected to the phenomenon of generalized para.- aller-gy, differing however in that leukopenia appeared only after 72 hours. The controls of this group presented marked quan- titative differences, which makes it possible to attribute. to the influenza virus exacerbation of the phenomena obser- ved? Pathologic histology of the kidney and lung of the.. rabbits subjected to the Sanarelli--Schwartzman phenomenon_ showed the intervention, of hemorrhages, thrombosis# lympho- monocytic infiltrations, blood extravasations, far more_ac-_ centuated in the rabbit sacrificed 4 days after the inducing injection than in the rabbit sacrificed after 41/2 hours,-.. The aspects observed in the lung and kidney were not obser- ved in the liver and brain. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 286 477OQ Al0BRONITKI9 AeDEREVICIV G.BALMUS9 PHAM SONG C otra? checornm in --i-- uui? k,ne action of histamin. St,cerc,4inframicrobiol. 19619 122 3, 367? Histamin administered in the form of aerosols to mice subjected to influenza infection brings about quantitative histopathologic changes in the tracheal cells, studied by means of the Panthier technique.. - .. The tracheocytogram of mice receiving,,) besides the virus, histamin and an antihistaminic (suprastin), takes, on an aspect closer to that observed in the mice that recei- ved only the virus. The control mice., receiving only histamin or tracheal washings with normal saline, did not present giant cells with a vacuolar contents nor catoplasmatic formations con- sidered by some authors as virus colonies4 Administration of histamin to mice inoculated with ma terial from an influenzasuspect case,. may help to reveal. the presence of the latter by means of the cytotracheogram study, according to the technique described in the paper, 478.c A?DEREVICI. Investi ations in the problem of influen4 za in the Rumanian People?s Republic bet. weep 195o and 196o*?Stecere9inframicrobiol. 1961, 12, suppl., 57? .......... _ . _ The main stages-?in the study of influenza during the 9 1950 1960 period are described The.results of isolation and typing of influenza virus strains in Rumania are reported, as well as comparative stu- dies on the various strains corresponding to the different epidemic_periods6. The author likewise describes the first antiluenza vaccine made in the country and the results of its mass application. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 0287 .a. 479 A.DEREVICI, AL.BRONITSI, GoBALMUS. The allergic factor in experimental influenza infection. The role of histamin. St.,cereo inframicrobiol., 1951, Ijb supple0 235; ReveSoi.Med. 1961, 6, 1a2, 33. 'The allergic factor in influenza infection was inves. tigated by exposing mice to histamin.aerosols following their infection with influenza virus. The disease was found to be exacnrbated,it was accelerated and the mortar lity rate was higher. This effect is definitely attenuated by the administration of an antihistaminic (suprastin)...._._.. Substitution of the antihistaminic by chlorpromazine (Largaotyl) did not result in an improvement of the diseaa. se, as expected, but on the contrary, in enhancement of in- fluenza infection. Virus rnu' tip]. i cation, as tested by hemagglutination in the lungs of the dead mice, is not influenced by adminis : tration of the above drugs0 This points to he presence of. neuro=hormonal disorders in the course of the influenza in- fection processo From those re .lt4 3 9 the existence of an allergic i'a~- for in the course of infuenza infection may be inferrede 48o AL.PETRESCU, PATH: NASIU0 Alte?ations of the tophofl dria, in white mice during influenza immunization0 St? Gem, inframicrobiolo, 19610 12,9 supple, 249e__ The authors have studied cellular mitochondria in white mice undergoing influenza immunization, _ as compared to the controlsa-._. _.v It -was found. that between the 5th and - the loth day- following immuniZati-one.no mitochondria-were demonstrable in-the tracheobronchic epithelium, liver. and skeletal mus 6~.e 0 This phenomenons _vhich is reversib,ie t is assumed to be due to changes in their biochemical c onstitutione Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 288 gjUI.VOICUI2SCU G.MARINESCU. Nervous involvement ill inf luny The third Congress of the Czechoslovakian microbiologists on respiratory viral disease* # Ma A G1. During and after the influenza ppa emic due to virus 'x20 38 cases with varied nervous ' "a-mI t were obeerveds 13 cases of serous meningitis, 7 encephalitis, 5 meningopncepha- litis, 12 facial paralysis and one transverse myelitis. . The etiologic diagnosis was confirmed by the presence. of inhibiting and hemagglutina.ting antibodies, studied dyna- mically by the absence of enteral viruses `in the cases with, facial paralysis) and on the basis of epidemiologiio finding; "4 The neurologic signs disappeared without sequellae in 2 to-12 weeks, except the case of transverse myelitis, in which a syndrome of motor spasticit; persisted. 4820= Y.OOPEWVICI, MLGRUTAO .BQSGA.T, n s ations on_, tb mumps vaoaine repared from au tochto ous. stra .nsv+8t sere. inframicrobiolo, 1961, , guppl?, 2070 _ A.. live ovovaccine against epidemic parotiditis has been prepared from three autoohtonous mumps virus strains after 26=31 passages on embryonated chick eggse Two types of vaccine were inveatigatedx a dialysed.vac, tine according to the method of A?A9Smoroddntsev and an undia~ lysed vaccine. As compared to the dialysed vaccine. the undia- lysed vaccine was found to have a higher imfectivity as well as a higher antigenic potential, as determined, by hema,ggluti- nation-inhibition antibody titration0 owing to its qualitieso as well as to `;.S simpler pre- paration, the undialysed vaccine is recommended for use in current practice Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 m 289 4 AOPQC0 483p? I ?ADERCA9 MGIANCC ACUe . GODANIELSCU. Investigations on. adeennovia' ._ .aq_--n lo- Isolation of adenovirus strains I _:i in the-Rumanian Pe ople ft s Republica St0cerc ,infra mic robin l o ft l96o a ll., 2 D 2290 The authors report the isolation of 4 adenovirus . strains on. human embryo cell cultures obtained by tryp. ,1nization0 Two strains were isolated from the nasopha-._ ry ageal washings of patient& with respiratory affections and two strains from the feces of patients suspect of a Coxsackie infection and presenting moreover a rhinophan gea;1 infection, The etiologic diagnosis was based upon .... complemeEE fixation and characteristic alteration of the human c. ,bryo cell nuclei in OQ in vitro ?D cultures, 6-4,Q M 0I OO SCU9 IOADERCAp Inves? do on adenova- ruse ll, Gel lesions prod,uce l b denoviruse . -4embryo cell ultureso a tocerc.oio-nframicrobjola 196cq llv 2v 237, The characters of the cytopathic effect produced .... . by adenoviruses in human embryo cell cultures were studied:, The lesions oboerrved. were similar to those described in other typAR of cells. The characteristic aspect of cell lesions produced by adenoviruses render them easy to identify.. 485,= AJ REVICI, M0ZSTRATIa Onmparative investigations on the diagnosis of adenoviral diseases m complement fixation and-M r el prop i itat$,on.0 St,ocerc0inframicrobiolt,p 19619 12? lD 630, Comparative investigation, for the diagnosis of ade- noviral, diseases, by means of complement fixation and agar gel precipitation of 243 sera from ?Ed.vi.duals with respioo ratory manifestations in the acute Stage or eonvalescone as well as from apparently healthy persons o showed a pro-. portion of 92085 per cent agar gel precipitation reactions Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 in the group of sera with the highest complement fixation titers (1/128) and a proportion of 13e,33 percent in the group with a complement fixation titer of The existence of a number of negative 'complement fi?. Nation testso but with a 7.8o per cent of positive precipi- tation reactionsg would suggest that the latter test might give earlier information on the etiology of adenoviral in. fections9 a fact that Mill remains to be proved by further dynamic investigations. Stocer o 4860- L.PREDESCUo P~qgress in adenoviral.diseaseso inframicrobiolo fl 1961 e 12 , 2n. 2330 A brief history of . the data in literature concerning adenoviroses is drawn up? listing these agents in the group of respiratory viruseso , according to the classification of Gorbunova and the W.H?O. The clinics and diagnosis of adeno- viral affections are then reviewedo The properties of the viruses are dealt with in detail. New data are.cited from literature regarding% the multipli- cation cycles of adenovirusesfl production of characteristic cytopathic effect after inoculation into tissue cultures of. deoxyribonucleic acid extracted from adenovirusess the onco- lytic effect of adenovirusesa their antigenic component and. hemagglutinating properties; cultivation of adenoviruses in tissue cultures'e Data are likewise presented on vaccination in adenovi' ral disease@8 suggesting vaccination of deoxyribonucleic acid extracted from the virus 9 according to '? r` owski who rocom- mends vaccination with pure RNA in other viral disease!,, 487,x- A GADsStc $,.NICOLAU a S.DUMITRESCU 19 N.?I ?IONESCU, UA.id Ti. AN e I.PACURARU Morpholog%an es in human_amnio= tic cells fected with adenovirus type Stpcerco.in? framicrobiol?? 19619 12.L 3a 289; Rev?Sci.Medo196196 9 3.4A kinetic study was carried out in the electron micros- cope on the morphologic changes produced by multiplication of adenovirus type 3 in human amniotic cell cultures,O cellular Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 2.91 - line F J4,0, These changes observed for the first time by the authors are present especially in the nuol.t? They are characterized. by the presence of complete.$ polygonal elementary bodies., dis. tr .b?}ted throughou tl.e nucleus and, protein crystslso, conside- red by the authors incomplete forms of adenov rus.O X88- L o L~ R CI 0 r D SGU Z .ISA.IA trjo ' s t3 ov . 0 an ibor es i various a e~ a ou, r amola t ~. ~opt~la on, of the l B-a~, i ..Peo le_a s Republic ? t -.r __ - - te cerc, inframiarobjola fl 1964. 12p. 3o 325w . . Investigations were carried out on antiadenoviral comple- r,en fi,ng antibodies in 699 ser^aj, considering as positive _ . tom, seaoa with titers of 1/8 and higher? The proportion of po- sl,tive saga0 classed according to age-groups was 29. per cent the 1?12 months age-group, 67 per cent in the 1-7 years a s.g.-ecup, 84 per cent in the 7-18 years age-group, 73 per-..- cent in the 1825 years group and 58 per cent in persons over 25 years old. The lowest proportion is therefore to be foal, in the,. J-12 months age-group (29 per cent)0 In the other age-groups the proportions.are about equal, the highest values being i .9 u fo n(' in the 7 - 18 years age-group (84 per cent) and in re-. . tits (8o,.2 per cent) fl a fact Which may be attributed to clo. ~~. contact under the living conditions of these rou g ps'o__ The number of isolations of adenovirus strains which, was smaller than the proportion of positive complement fixing reactions, points.to the utility of this test for a retros- opeative diagnosiso . i,89 e- G Q f NESCU O . D JA g GeISAIA0 L. PREBESCU J: Y?CO PELOVIC I . LOIOBANESCuo Amite obstructive laryngitis in the tour- se7, of infections due to viruses from. the APO Zrou ? St? cerce $ inframicrobiolee 1961., l2 fl 3?~.y~7 Five acute obstructive cases of laryngitis are reported out of 12 laryngitis cases caused by infection with viruses, of the APC group:,, There was a definite croup sy ptomatology Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 in all the children under the age of 2.. in one child aged 5g who had croup for the third time o and a milder fora in an older child? The diagnosis of APO infection was established by isolation of a cytopa'Gfl genic agent belonging to the ad, -. novirus group and by kinetic study of complement fixatio,e 49o e A?D VICI 9 AL?BRONIT 0 LoPREDESCUm lnve sti at ::.s on the etioJ~ogyof soae focal respiratorvir .l d, r es in different cen s in t h'2 count duri t ho first half 2j _136o# Microbiol?g raraZitol,e g epidemi ':= ,,:, 19619 6g 499e. The authors show the result of investigations carri ~F?~ `k r~ tr. SA ~ > i`?t` C` .. ~^. -.,... w r . *^~*~.*.. ,..:...".~.s:.aeu ak ap:t a..w ~` ve para11e1~ exper meat s . ca ri,ed o oa r58 piglets showed that. adsorption on aluminium hydrox ie.;doee note n- ? fluena.e theF~ d f~ferenceLoe that exist between the immunogenic k iJ . ~~.w . oFM?~evt ~~ value:Rof:~virul~en ex~traaz ~ of liver-or bra no a A a ?.! V wi#i1 i+T. Y' t Y.1i .11tY '. ~.2 iL:1 Af~te:u adsorptionjjon;aluminium hydroxide gel,T the vac ~ ? ~. z ~~~.~ x~ .~. awn a~ ~. e-~ passd fromlaad,,:brain {'have a'' ~:~ tines. re. ' r tt.lower 4mm6pogenia ~ ~ ~ T.1 `~4?.:.'.~ 3 M ~Vl fie.. +T y i ~. value .-than he.~ adsorbed, extracs of formolartR ii spleen. v, t R l3 BAg;.compareed,,t Cthe immunogenic value of .the. yadsorbed .} a ati ' . /e+.:Y 1 splenic~. extract,,. the. ,value , of the adsorbed he{patic extract { repro-senta>lapprcLx mately`5o bper, eent.~~andt,adsorbed brain es-l tract on4lty about? 20;oper Beat lof,rythe Amkunizing.,,value of the adsorbed'e err 521:-{ VI-sWYNC'RR'ADN Kc L. ~; I.I GRIN Y, I.GI QRGRIU, N?BIRBU. a dteric1dal-_action_of. tri paf1 the .an d' acr flAv'lne to asteurella ink;the' bls .d ue~l.d~foP.~h rperim izar- j 0ption*1'6fLranCAJAL. Laboratory dimosis of some ,yagayellas y (benign lym ranulomatos s.. trachoma and-viral, prima atypical eumohia) b y " ieaihs of the WeilmFelix tes e S t - e - mic-ro' Viol" 1951. 22,, 1-49 3510` Based on the presence of certain antigenic fractions common to the pararickettsia group,astudy was carried out among other tests also on the Weil-Felix test in some miyaga. wallas (benign lymphogranulomatosiss trachoma and primary atypical pneumonia)" The investigations showed that the Weil-Felix reaction is positive in trachoma, irrespective of the stage of the disease, in benign lymphogranulomatosis, especially in the inguinal forms, and in a great number of atypical primary pneumonias. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 - Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 .~67 590-- N.CONSTANTINESCU, C.DRAGOMIR, E.DUCA, A.VITA, A..DARINQA , D.CIOCHINA. Experimental investigations on " . " fever in Moldavia:" St. cerc., inframicrobiol., microbial., parazitol 1951, 2114,99 Several strains of Rickettsia burneti were isolated from foci appearing almost simultaneously in the month of April, in two areas in Moldavia. The rickettsiae reproduced the characteristic infection in the guinea pig and were cul- tivated on the yolk sac of embryonated eggs. Cross immunity reaction establisho,d antigenic identity between R.burneti in Moldavia and R.bur?neti isolated in other regions-,of-the country. 591- Conf.A.STROE, C.MUSETEANUq V.CIOCIIfTEU, G.MARINESOU, . Sp.MIRINESCU. Typhus fever in Children. Report read at the Section 6fr'Medical"Sciences"of'. the ~R.P.R. Acade- my on December 30,-1951. Clinical observations and laboratory data obtained in 393 cases of children suffering from typhus fever during the 1946-1947 epidemic, confirms the opinion that this disease is usually benign in children: moderate fever, short dura- tion (less than 12 days), discrete or no eruption(15% of the cases), few respiratory-and cardio-vascular complications, moderate neuropsychic signs. Splenomegalia was observed in 6o % of the cases, en- larged liver in 25 % of the cases, abnormal urine analyses in 2o %, physico--chemical modifications of the cerebrospi- nal fluid in 78 % of the cases. The Weil-Felix reaction was more often negative than in the adult. N? lethal cases were reported.' Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06 CIA-RDP80T00246AO19200640001-8 5920- A0STROE, C0MUSETEANU$ ?"OCIOCII/EU9 G.MARINESCU,SP-.MA. RINESO'U Clinical and laboratory study of Bole fever patients during the 194 1 7 epidemic. Report, read at the Section of Medical Sciences of the R.P.R. Academy,on December 3ds 1951. A detailed clinnco-statistical and physiopathologic study was carried out on a group of typhus fever patients (8ol cases),during the 1946=1947 epidemic. New data are pre- sented in connection with blood pressure ...and.modification of the cerebrospinal fluid during typhus fever. Moreover, the' statistic shows that the fever lasts 14 days, as described clinically,only in 15 to 2o% of the cases. Fever does not de- crease suddenly, but slowly in 3-6 days in 8o% of the cases. The eruption apDears in most cases on the 5th'day of. the di- sease and is often absent in severe cases; it can ' aptear on.. the.face. palms and soles, as in Mediteranean fever ( 13 out of 8ol cases) . The authors describe a syndrome,the so-calles "pressor- effect" on the 9th day in some of the to tients presenting before death a rise of the blood pressure together wit . aggravation of the neuropsychic symptomatology. 5930?N9CONSTANTINESCU,C.DRAGOMIR,E0DUCA,M.DUCA,Z.CARAMAN,--. AOVITA, E.PAI,ADEC. Strains of _meningo?neumotropic viru- ses isolated in man and micee Bul0Stiin#;.Sectia Med. 1952,...4,.1, 321._._ Virus strains from patients with lymphocytic meningi-. tis and from a mouse, caught in the home of one of these pa, tients,were isolated on guinea pigs.The.pneumotropic activi- ty of the virus was made evident by wide-spread foci of ami- crobial monocytic pneumonia, as well as by inclusiogenesis with 'jasophil inclusions in the cytoplasm of the hepatic cells. The totality of these properties together with exclu- sive culture on the yolk sac of the embryonated egg, made it possible to correlate these viruses to those of the.miyaga- wellae family, the psittacosis-lymphogranulomatosis-pneumo- nia group. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 A . P, !T.' rll r,1 592',-..R.BRAUNER, H.LACK, H.ANGELESCU, V.NICOLAU, A.DEREVICI, A.CIUCULFSCU,-D..SARATEANtJ I.PANDELESCU, M.ERACLIDE*' T.PASCU, I.SANDU. Study of acute'"pneumopathies,Works of the Second session of the Medico-Pharmaceutics Insti- tute, Bucharest, 1956, 90. In order to establish the etiology, diagnosis, therapy and prophylaxis of certain acute atypical pneumopathies, the Hirst test, cold agglutination, the Weil-Felix reaction and hemogram are applied besides other diagnostic means. In most cases, an influenza etiology was established, with a'peak incidence in autumn and spring. The influenza vi- ral etiology of certain fibrinous pleuresies'-was likewise established`; 5950- C.IACOB._Beni inoculation phoreticulosis " Protle- me ? de Infrariliorobiol" EdMedic, BuoareOti 1956; ; j~. Recent data are given on this new nosologie entity, for which R.Debri_proposed the designation of " cat's claws di- sease " and P.Mollaret " benign inoculation lympho-retieulo- sis. ", "in 1950, A description is given of the epidemiologic,' etiologic and experimental aspects, symptomatology, clinical forma,positive and differential diagnosis, histologic modifi- cations, and ending with therapy.' A fact stands out in literature: that anamnestie inves- tigations of the 300 cases reported up to 1954, establishe d in 83 % of the patients a single or repeated contact with cats. Relatively frequent epidemics are reported in one fami- ly or in a building, showing that the cat is a virus carrier and eliminator , without however presenting a manifest sympto- matology. The possible geographic spread of this new viral disease is' very great. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 :.- . Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 37o ? 5960 A@GIRCU), G,MARINESC,-,. Ocular and nervous reactions caused by infection of neorickettsiae or elements of psittacosis group into the anterior camera oculi? C.R. des s4ances de lSAcad. Sci.1957, , 2433-2435 Inoculation of a virulent suspension of neorickettsiae into the anterior camera oculi of the rabbit gives rise to an almost total infection of the eye with lesions, particularly at the level of the cornea, ocular choroid and papilla, The reaction.. of the nervous system is manifested by meningo- encephalitis, with marked vascular modifications. 597e- P.GIRCUD, G.MARI ESCU. Hepato-nepritis in new-born rabbits-due to neorickettsial 'and certain' elements of the psittacosis group, _ C ?R. de( Sdances de l'Asada Sci?_ 1957, 16 2566, The harmful activity of neorickettsias, multiplying in the'animal organism, is manifested by various deposits at the level'-of the blood vessels and viscera.%. (lung, heart, liver, kidon~,1 , spleen, brain). The most important histopa- thologic modifications were fund at the level of the kid- neys and liver, with prevalent degenerative lesions* which indicate the toxic action of certain neorickettsial strains. 598.- N.DRAGANESCU. Studer of inclusion-like eosinophil for. mations within'"the cell cytoplasm in experimental neo rickettsial. pneumonia,.. ComunicArile Acad,R.P.R. 1958, ~'g .1 l.. ?. 23 Applying the Mann staining method, the author describes in the o .plasm of the alveolar and mononuclear cells in neorickettsial pulmonary infection eosinophil formations closely resembling inclusions. The presence of these forrmations in mononuclear and nervous cells, in experimental neorickettsial meningitis in rabbits, leads to the assumption that they are the final re- sult of the struggle between the cell and the inframicrobial germ Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 ? 371 Experimental neorickettsial 599. N~.p~RAGANESCU, HUNG TA4. E~_____ menin As in rabbits: Comunie 6rile Acad,.R.P.R. ,1958, 8, 129 1353: It was possible to reproduce meningitis,by suboccipi-= tal intraspinal inoculation to rabbits of a lung suspension from mice experimentally infected with a neorick?susstrain, isolated in Bucharest', i An infiltration with mononuclears and polynuclears observed under the microscope at the level of the meninges. Some of.these mononuclear cells present intraeytoplas- mic eosinophil formations, identical withc- those described in the cytoplasm of. alveolar and mononuclear cells, in ex- perimental. neorickettsial pneumonia. Perivascular lesions, inflammatory nodules and the presence of eosinophil forma- tions within the cytoplasm of the nervous cells is observed at the level of the cerebral parenchyma, subjacent to the meninges`: 6oo.- N.DRAGANESCU, D.S .` EANU? Serologic identification erm ., isolated-from" a of - a virulent Ae orickettsial! perm-isolated-from" rami= human case of ati~ is al .. np eumonfiaSt:. c e re 3af crobiol ?mierob of "OparaZitoi., 19589 # 19' 61' Positive micro-agglutinations performed with antisera prepared on rabbits against the Giroud antigen, as well as the morphologic characters and cultures of the virus,, fully justifies the assumption that this germ isolated from a hu- man case of atypical pneumonia belongs-to the neorickettsial group described by Giroud. e resense of neoricket-~ 6o1'.- N..DRAGANESCU, D.SARATEANU., Th ...r L2r_e se trial germs in the Rumanian" People ? s Republic.. Study of a neorickettsial strain isolated from- a humag case _- = of acute pneumopathy. Report read at the" Second Cog ference of the Czechoslovakian virologistsq Smoleni- ce?Bratislava,Oetober 14 -17.,1958. A study was carried out on a viral agent isolated in Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 372 the R.P.R. and cultivated L yolk sac of embryonated eggs and intrapulmonarily in mice and the cotton rat, inoc u-? lated by intranasal route. The round or. oval isolated germ was stained red or blue by the Macchiavello staining method, on smears from the yolk sac and lung impressions from mice and cotton rats. The positive serologic results in the micro-agglutina- tion reaction show that this virus belongs to the neoricket- tsial group. From the microscopic view-point, the prevalent lesions in mice and cotton rats inoculated by intranasal route are localized in the order of their severity, at the level of the lungs, kidneys, meninges and liver and are constituted of lymphocyto-polynuclear infiltrations, stasis and hemor.- rhagies. Inoculated intraspinally to the rabbit, this germ causes meningo-encephalitis. The eosinophil formations in the cytoplasm of the al- veolar'and mononuclear cells in experimental neorickettsial pneumonia in mice and cotton rats, as well as in chick em- bryos inoculated in the yolk sac, seem to be the final re. sult of the struggle between the organism and the germ. 6o2,6- P.GIROUD, G.MARINESCU. Oculoenc o,1t tics lesions caused-by various neorickettsial.strains`;,' Bull. de 11Acado' Nat. Mede.958, 1421 5-6, 135. On the basis of certain clinical observations regarding ocular complications in several diseases caused by neoricket- tsiae, 16 rabbits were inoculated in the anterior camera ocu- li, uni- or bilaterally, with various strains (X14, V14, L179 and Q18) isolated in different regions, from cases with the most varied clinical syndrome.. The clinical and anatom.ccpathologic study showed the presence of a quasi-total ocular infection (kerato-conjunctik. vitis, uveitis, optic neuropapillitis), with fairly important vascular meningo=cerebral reactions. The toxic action of neorickettsiae multiplying in the Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/1.2/06: CIA-RDP80T00246A019200640001-8 m 373 the zeningo-.cerebral-modifications. anterior cameraY oculiexplains both the, ocular lesions and. On the basis of this study, an? analogy was ' drawn bet- ween the 'ocular and nervous lesions observed in rabbits, and the loculomcerebral manifestations observed in man during the course, of._the:.:_diseas&? 1958; :.I42V"i7 18~' 77e :' types` of n` eorick'ettsial' strainrs; ? evidence of hep tone`- phritiein ne.*-.born-rabbits eBuli ~de`,lAcad Nat Med:r 603, P.QIROUD,'`' G MARINESCU , M=?me `N.DU . ' Toxicity` of two' Inoculation .to new, on and suckling rabbits of, various., neorickettsial strains, isolated from sick animals and .peop;le tiind cultivated inlungs ' or the yolk membrane of the,, embryo- it ted' egg, gave rise to ''important hepatic and renal lesions. similar to those observed in renal pathology. 6o21-.= ? D.SARATEANU9 N.DRAGANESCU, ' R.PORTOCAI,A, M.CEPIEANU, N.IONESCU. Isolation` of a viral' agent -'at ' the:'limit of the r etts rou from a--case`.'of"at pical ieumoniia . St, c e From the sputum +. of~a patient with iatypical pneu monia, a viral agent was- isolated' by" Inocul ation into the yolk. sac of 7-12 day embryonated eggs, maintained at 37o and334?C; they,. Are' able to transmit to mice. by intranasal route a serial,ly. transmissible lethal disease: f th M ebiavello staining method --polymor~? a B +-"k.0 -non['-1rt~ttA to OrouDr y means o phous' corpuscular`"formations1 2oo-5`66 in size., staining red and blue, were revealed`on'Ythe ti wears obtained from the yolk sac of? the inoculated eggs; and ;on mouse lung: impre$-,.. ; sions on the basis;. of serologic examinationsof the patient` and the morphologic orl cultivation charactersof the virus, it is' considered that the yisolated age msy ,be classed. in ? K ~ .l "? _. i .....t v ~.. ... ~ V ...~) ~ ..?, t ... 7 v as L Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 374- 605.- D.SARATEANU, EL?OPRES T., N.D.RAGANESCU. Isolation of an inframic.robial agent from the Psittacosis-ornithosis group during an epizootic in ducks. St., cere.,. infra- microbiol.smicrobiol., parazitolo. 1958, 11, 4, 417. During an epizootic in ducks a'virus was isolated which, inoculated to mice by intracerebral, intranasal and intrape- ritoneal routes induced a lethal disease. In the smears from the lung, spleen, liver and peritoneal exudate, the causal agent appears polymorphous, staining red and blue by means of the Machiavello method and localized in the forffiv of intra- cellular particles or extracellular clusters, Inclusions are to be found in the cells of the alveolar epithelium. The pathogenic and morphologic characters of the causal agent led the authors to list this virus in the paittacosia.- ornithosis group. 6o6.- D.SARATEANU, N.DRAGANESCU, R,PORTOCALA, N.I,IONESCU. Neo rickettsial 'pneumonia. Isolation-and--ideiitification of the causative went. Miorobiol., parazitol., epide- miolog,,1958, .. 6, 529, A case of acute pneumopathy is presented, from the spu- tum of which a germ was isolated, serologically identified as belonging to the neo-rickettsias described by Giroud and asso- ciatese This germ inoculated to mice by intran.as4l route, indu- ced pneumonia with microscopic.lesions due to lymphocytic and polynuclear infiltration and necrosis. 6o7.- D.SARATEANU, N.DRAGANESCU, EL.OPRESCU. Isolation of vi- ruses other than the influenza virus in'pulmonary s dromes simulating influenza. The Third-Unional Conferen- ce of Influenza9 - 25'_ Sept; 1958, giew. The investigations carried out Confirms the participa- tion of the ornithosis virus in the etiology of atypical pneu mopathies and draws attention to a new group of viruses, the neorickettsias, which can induce the same clinical syndrome. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 375 6o80- R.BRAUNER, N.UCK, N.BUCUR, D.SARATEANU, N.DRAGANESC1J. Considerations on some cases-of ornithosie''St. cerca, inframicrobiol., 1959,.2. 1, 31' 81 cases of atypical pneumopathies, 8 of which are considered as ornithosis pneumonias and 25 'as suspect of ornithosis, are described. The clinical aspect of ornithosis is presented and attention is drawn to the existence of ornithosis cases in our country, 6o9.- PRO.F,V.BUSILA, I.VASILESCU, A..FITARAU, I.MONTAG (Clinica de Boli contagioase- Timigoara); D SARATEANU,-EL.OPRESCU-MSSIEVICI, EL.NASTAC (Insti- tutul de InfraMicrobiologie, ' Ace,d:R.P:R Bticure$ti). Contributions to the clinical-study of ornnthosim. , Microbiol: para~itbi:~"epidemioi 1959, 3 132; The case records presented illustrate the diversity of the' clinical forms, the intensity of the pulmonary spn.. drome and the difficulties of differentiating, on the basis of clinical criteria, ornithosis from other viral pneumopa- thies. Diagnosis may be elucidated by means of serologic reactions or by isolation and identification of the causal agent. In the cases described, diagnosis was made by means the complement fixation test. 61c,e- D.SARATEANU, N.DRAGANESCU. Experimental transmission to the cotton rat of a neo. rickettsia) germ isolated 'from a human c a4e of atypical pneumonia,** " .. Acta. Viro logic a " 1959, , 1; 46... It was possible to transmit lethal neo--rickettsia) pneumonia to the cotton rat (lethal 95 hours after inocula- tion), by intranasal inoculation of a suspension of yolk sac or lung of mice which had died following the experimen- . tal disease. Predominant microscopical lesions were to be found in Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part-' art - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 - 376 the lung and consisted of inflammatory, oedematous and con- gestive lesions. In the rest of the oz} ...._j s lesions of circulatory or- der were to be founds the inflammatory lesions being discre- te or 'absent. By means of Giemsa staining, the presence of neo-ricket- tsial elementary bodies was revealed both inside the alveo- lar and bronchial cells and extracellularly. 611,- D.SARATEANU,.H.TAO, Gh.MARINESCU. Experimental ornitho- sis in hamsters. aper_ rea _. a the Scientific Festive Session of the Institute of Infrazicrobiology of R.P.R. Academy, 1718 August,19590 - Paper read at the Interregional Conference of Morpho- logy Tg.Mureg9l8-19 December?1959 Experiments on 93 adult hamsters and 58 sucklings showed the morpho-pathologic modifications produced in this animal following inoculation of the ornithosis virus by in- tracerebral, intraperitoneal or intranasal route"4: From the data obtained, it results that the histopatho- logic criterion cannot be used for differentiating ornitho- sis virus strains 612.- D.SARATEANTJ, E.NASTAC, B.FUHRER, EL.OPRESCU, T..HUNG. Investigations on ornithosis virus-'infections in the Rumanian People's Republic. St. cerc., inframicrobiol., 196o, 11, 19 73; Biull.Nauc.Inform0196o929137; Acte Viro1.oUng?196o,Z,2,l860 Eight ornithosis virus strains were isolated from ducks(5)0 turkeys(2), and geese(i), pathogenic for the whi- te mouse inoculated by intranasal'route, and less pathogenic when inoculated by intraperitoneal route. These strains were also pathogenic for the(. chick embryo inoculated into the yolk sac or the chorio-allantoic membrane.. The antisera, prepared with the isolated strains in cocks, produced agglu- tination of the specifically stained antigen and gave posi- Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 377 tive results in the sercneutralization and complement fixa- tion tests in the presence of the specific antigen, permit- ting identification of these strains as ornithosis virus strainse The morphopathologic alterations produced following inoculation of the ornithosis strains to the white mouse, suckling and adult hamster and irradiated or non-irradiated white rats, were studied,' In order to detect ornithosis infectims in mans in- tradermoreactions and complement fixation tests were perfor- med with the sera of patients presenting a clinical syndrome of PAP, caretakers in bird-breeding farms, the personnel of the bird slaughter houses, as well as pigeon and parrot breeders, etc. These tests yielded a high proportion of po- sitive results (3o.9%-..833), demonstrating the wide spread of ornithosis infection`in the country. Intradermoreaction is a sensitive, early and specific test, that permits detection of the infections even in the first 5 days of the disease. Complement fixation is more sensitive in human cases, and the authors stress the necessity of using double or even triple serum samples in order to obtain conclusive results. r013.- I.BERLOGEA, I.STRATI, S.SCHOENFEID, D.SARATEANJ GH.MXRINESCU; W.T.LIAN: Data on ornithosis infection in the ' irradiated and non-irradiated white rat'*' St. cerc-e inframicrobiol. , 1960,E 11, 19 111. An attempt:was made to transmit.ornithosis to the white rat, a species that is resistant to this infection, by using ionizing radiations in order to overcome the na- tural resistance of the animal. The investigations were caried out in 47 white adult rats, that received a dose of 51orrat each exposure. After 3 days, this rats were inocula- ted by intracerebral or-intraperitoneal route with duck or- nithosis strains, The experiments showed that the lethal disease cannot be transmitted to the irradiated white ratj,, which presents Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 =378- no c linic a.1. k . _,~1m the d::_.: e ? Complement fixation with the sera of irradiated animals yielded titers twofold lower than those of the non-irradiated animals. It is considered that the dose of ionizing radiations used was not sufficient to modify the cellular systems in the rat, so as to satisfy the synthesis requirements of the ornithosis virus, for re- producing the experimental diseasee 614?- V.TOBUSIIA, R.ALEXANDRESCU, D.BACALOGLU, D.SARATEANU, EL.OPRESCU-LISSIEVICI. Clinical and morphopathologic aspects in an mic dtae to! ornithosis virus St. cerc., inframicrobiolm, 196o, 119. 2 _ 187 '_ ,...._ _ Investigations were carried out on an epidemic due to the ornithosis virus that broke out in a duck breeding farm, affecting the 3 caretakers and the watchman. Subsequently, to these cases.another case was added - the mother of one of.. the caretakers, in which the infection is explained by direct contact. The clinical diagnosis was confirmed by complement. fixation that was positive for all the patients, and by iso- lation of the virus. The epidemiologic survey showed that the..epidemic had been preceded by an epizootic among the.. young and adult ducks. A single human lethal case was regis- tered; the work relates the more important morphologic as- pects observed at necropsy and at the microscopic examination, 615.- D4SARATEANU.,.E.NASTAC, V..T..BUSILA, A.ALEXANDRESCU. Laboratory study of an epizootic due to,the ornithosis virus. followed .by.....a human, epidemic. St. cert, infra microbiol., 196o, l.1, 20 195. _ .__ .,The_oxisteiice of.-.6h.ornithosis-..epizootic--in..ducks.-, followed..by._.5. human. cases, is reported, showing once again the possible transmission of ornithosis from birds to man and from man to man. The presence of antiornithosis agglutinating antibo-. es in the sick, convalescent or appa;ently healthy ducks, and of complement fixing antibodies in the sera of human Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 !I I 379 d~~"'1 I" ems.. u..,_. ~Y,. ,..4 ,4, .: ?? ~.. le ,. Iogyi,of the~"epizoot c aud' epidemic studied;o:t., cases, as well as isolation of some virus strains from birds. ~, ?, r. ~: ,- r ; fit' . and a lethal- human'case, ;_, -~po;in"te,rd,' to a common' ornithosis etio- ' 616.- G.MARINESCU9 D.SARATEANU, T,HUNG. Comparative studyA... of 'fhitolag alte'raticris `in white,rats inoculated with _ "various auto'chtonous ornitnosis'. strains. St.: cerc,, in- framicrobiol '1960 ? ll '2 201: The authors describe ,.the ,evolutive .:aspects. of, then his- tQpathologic picture followinoculation, to.:the.. adult wrhte: mouse of 3 different ornithosis strains isolated in the.R,P.R* by various routes.. . Inoculation .of these, strains by intranas ;al route. indu- ces character ftstic`ornithosis ,pneumonia. foc i. The TC strain- (turkey) gives rise sooner to interstitial, andalveolarlpro- cess'es-=C3' t6-1 hours) than the'CR ' (duck) end TG( o V ? The r stra ticulo-eadotheiial system-were noted. at" the level of the the v.rhite mouse 'following" `inoculation by iltraperitone al rou- to'o - Stasisa degenerative lesions, and `hyperplas a of the re- .d" manifest a low patnogenici` v for phocytc''menngo-encephalitis even after intranasal inocu Intracerebral inoculation produces lethal 'meningo I en- aephalit:s The' TC 'strain (turkey) produced serous or lym 'Element'ary bo'dies were found''in all' the organs:, lung (infiltration molionuc'lears) 9r liver and ''spleen '(reticuloen- (alveolar cellso bronchic cells and macrophages),'b?rain'. 61 -' DoSARATEANU'9 'T~, UNGy Go IEESCU. Experimental orni- 1-29 1-29-169," Three ornitho,sis strains were inoculated,,-by,intralna.- i.ntraperitoneal and 1ntrac.erebral route to. 93. adulat , 96ol. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80TOO246AO19200640001-8 380 and 58 young hamsters. The 7e tilts obtained point to the . possibility of transmitting the lethal disease only to young hamsters and only following intranasal or intraperitoneal inoculation. ... The present paper describes the morphopathologic alte- ration$ produced by inoculation of the strains by various . . routes The histopath.ologic criterion cannot serve to diffe- rentiate the strains. Irrespective of the route of inoculation used, the strains presented marked pneumotropism. 6l8b= TeHBNG. Experimental and moophopath.ologic inves- tions in the psittac-osismornit o is g. roizp.4. Ste cero. inframiorobiol., 196o, 1~,, 3, 425. The present paper deals with four experimental inves- .a. tigations carried out in the white mouse, adult and young._ _ . hamsters, chick embryonated eggs and rabbits, with the o'nii thosis virus strains (OR, TO and TG) isolated in Rumania _ The various staining methods used and studies in the optical and electron microscope led to the following results:.... In the development of the ornithosis~ virus it was not.. possible to confirm the existence. of the evolutive cycle des- cubed by Bedson and other authors, From the moment which the virus penetrates into the host cell up to rupture of the.. cell, the.. virus is to be found in the form of elementary bok'o*i dies, that can be modified, agglutinated, enlarged by the de- fence action of the cell. The presence of inclusions proper was not observed,@_ since they were formed of individual elementary bodies. In.. the electron microscope, large elementary bodies with a lO density and small bodies with a higftdensity, . were observed.-. Apart from the cytoplasmic ?'alterations, examination in-, the electron microscope also showed alteration of the nucleus. The presence of viral elementary bodies in the endothe- lium of the small blood vessels and infiltrative elements, proves dissemination of the ornithosis virus by hematogenous route Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Intradrermoreaotion is a sensitive, specific and early test, which can detect ornithosis infection in the first 5. days of-the- disease. Ae iplement fixation is just as species fiat but not so early or lasting as intradermoreactiono ornithosis strains. -Marked pneumotropism of the strains studied was obser? ved , irrespective of the animal or route of inoculation used. The morphologic criterion cannot serve to differentiate the o 619. D.SARATRANU,, '-psittacosis?-~ornithosisoGene ralities his. tors, eharaoters of the viruses methods o~ diagnosis.St0 certo inframiorrobiol. a, 196o v 11, 3 p 445 The author reviews the data in specialty medical literate tire referring to infections produced by ornithosisppsitteieo4 sit germs, and especially to nomenclature,, taxonomic clas j fication and history of the disease in mane The morphologic and Immunologic characters of the viru- ses of this group are described, as well as the methods of diagnosis. The value of various diagnostic serologic tests._ and the way in which the results should be interpreted, are discussed. The economic and epidemiologic importance of pararicket. taial infections in various mammal and bird species demands the organization of a systematic study of these affections in our countryo ced by pararieketttsias in various mammal speciea'o. 62o.- C@SURDAN, G.8ORODOC. Infections in mammals produced b inframicrobee of the pararickettsial group. 8ta cerce~. inframicrobiolo a 196o, 11, 3;; 4590 -----The-most_.recent bibliografic data-regarding infections Produced_by_germe-.of the- psittaeosise ornit ..psis.lymphogranu. lomathosia-group are.reviewed.-The-authors-briefly describe the anatomocclinical,, ?pizoatologic . characters- and- methods. of diagnosiao,,and...tho-control ? and prevention-of-diseases product Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 General considerations are made on unification of -. the e tioiggic nomenclature, the best being that proposed by Aoa&.oSt0SoNicolauo Opinions are likewise stated concerning the designations given to the various morbid entities des. c '4bed by different autho 5 in the same or different species which have factually the same etiology The authors raise the proble of mixed pararickettsial and bacterial infec4 = Lion; fl that have been considered up to the present as ha vies a bacterial etidlogye In the investigations wader way, an inframicrobial agent with characters e3 la to those of the pararickettsias was isolated from cases of swine enzootic pneumonia, Similar ger were also isolated from cases of eua and calf pne a.oentv. 'i.t ,a U 621oa DOS.ABATEAMU9. NoNASTAC Bok?tJHRERO. EL.OPRESCU9 C.T0HUAI9 12e1 C o C aratiye investigations on the diagnostic, vale of complement fixation and of intradermoreaetions t ors i,thokav-' 196o, 3.. 2530 Mierobioloo parazitol?s epidemiolo e By using 3 ornithosis strains, in intratracheal moon. latton the authors induce disease in rab'bits' in Which. they-... s,ubsequc~ntly test the comparative value of the jntradermoreae tton (IoDQR?) and complement fixation teat (C.F*i' 0. Aceor ding to the data obtained, in 7 human cases -1nYestigated--__ there. was--a--concordance of - ?2,. between the CeJT.- and the l 4,DvRo ...while -yin the experimental :vest,igations .the --concor- de oe-was of .14e.%%o--The_.CeP T.. a .ume..to-be .more-sensitise than the .L.l ,,Ro .. in .h ca e fl ity. inde* ofx 5l.*$ being obtainied with the former aiad o'- wit; the latter.- 'The experimental investigations ~hh;ow d the 116De2r to be _a_ . sensitives specific and early test-: . affords the possi ? bility to detect the infection ; as a.aof. as during the first 5 days of the disease a Repeated intradermal inocuiation- of the antigewl does not cause the formation.-of coleme t fi- xing antibod_iseco In a sensitize or an :i .p, tbo o ?.R may play the role of a booster inocuiatic no The *:Ce,F.,To is just Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 383 as specific, but less early and persistens than .the IOD.Ho 6229b D,BARATE NIJ, T.HUNG, BoDUMITRE8CU, NoI oIONESCU. of the behaviour and mor ho atholo c alteration _pro. duped b ornithosis etrain? in the chorio-a lanto c membrane of the developing chick embM~, Sts cerc0 in_ framicrobiolm, 1961, 129 It 830 Investigations were carried out on the behaviour axtd_ morphopathologic alterations produced by ornithosis strasi in the chorio-allantoic membrane of the developing duck embryo. It is shown that propagation of the ornithosis infection to the unaffected tissues also occurs by circulatory route? Histologic alterations are initially due to the toxin and subsequently to the action of the virus. The histopathologic criterion cannot be used to di-ftsren~- tiate the strains with a low virulence, inoculated into the chorio.-allantole membreaws . - - The presence of viral elementary bodies; of various as-. peets and sizes warn demonstrated on thin sections in the elec. trot microscope. 6234,m ACAD.BT.81.NICOLAU o 9ardiova ulcer Ifections induced b~ i a .crobese at ceres inframicrobjolo, 1960$ 49 619p - - The author relates the observat ons0 experimental interpretation8 and Conclusions-of prof op. Gjroud . and co, Wor ra, in _tha.prob1em-of..caa+diovaecu a affections produced by in ra microb :The works.`of-prof P..Giroud have proved-that'" nume _ roue-cases of thromboarteritis._obliteraae., and..cardiac;;:,infarct are caused bar ricketteiao.=pararickettsjas and, neorickettsias. .1;~ L.. e. sp demiologic . clinicalaand.histopathologic - "ind s' ands ? cpepimontal data refesrrin~g. at' :the :anal et oa and~ider tif"io cation of inframicrabiaL germs, V and the results of.s olog . . investigations in various card.ova,ee are affections b a .?,~ , ria?d.- Ora ohs b~ssie~; o the~da a. reeented.'-the au d 3 =ma ee a series of ad8. 'trio nah considerations on the importance of Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80TOO246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 the tests usedy. interpretation of the results# t therapy and wide prospects of the investigations in tb..I fields which will contribute to the preservation of . health@ recovery of numerous arteritis patients and the pre -vention of cardiac infarct or coronary thrombosis, affections that are often fatal,, at stimulating the discussions following which definite 624,0- Vo TeBUSILA . D SARATEABU m OniheYM~wY.?prevention of ornitho= aY.~Yw ~M4 Y~ .Y .W. . ^ Mi ~Ir~~,G~ si_. deduced from a izootolo ii an:d a idemiolo i data. Mierobioio parazitol.% epidemiol?, 196o, 5e 4o5? The data in literature on the epizootologic and epidemio- logic aspects of ornithosis are reviewed. After analysis and discus@ion of the means of control, certain measures sioe_re- comets d that might interrupt the infective chain, These-_ measures are addrease : to the source of infection.,, the recep- tivre kmss and, interruption of the routes of dissemination. . The proposals are deductive to a great extents there.--- f ors theoretical and perhaps difficultly applibable,, -but -they novc will be established' . 6250=1 D SARATEANUa O. SU'RAANQ GeSORODOO0 B.P'U .ANAGNOBT3w .- _ _ sol at.3o of infamicrobe from~ a IYIfocourlilYlYof .-ewe-enzostic ~Cp? 4q .wY YY.rF.-tiia.r 7YI4r111 Y~i1.~IYY M~.~pi-+~.Y Yr~11IriYeborti ono Stv came Infrsmicrobio]. *v 1961e 12a1f, 95+ MMM. . _._ __An . inframic,rcbe was isolated c oneomitt'antly with Sal. moxaella_ abortus_. ovia from a focus of ewe 'en? ootic abortion ~- The_.i.nfr .microbe- grows _in-the yolk sag of the .' -days old-chick embryo and-is-pathogenic .?. fqr the mouse inoculated by intrana- gall or in'tracerebral route, In smears from the yolk sacs. and from mouse organs, _ -. staineL by the Macchiavello methods the intra- and. eztraee1 _ lular elementary bodies have a polymorphous aspect, are large ex4 stain red and blue .The epizootologic character of the disease, the morpho4 logic o tinctorial and ims.unobiologic properties of. the isola- ted framicrobea led to the conclusion that it was similar Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 to the ewe enzootic abortion virus, listed in the ,rnitho- sic poittacosis group. 626.- AQAD? BT,S,NICQLAU,, . C.$URDANa n.S4RATEANu, Pf.ATHANASITj,. B.FUHRSR?-ANAGNOSPE, C ILIESCU, R.RADRSQU. Viral. etiol4.? in the field of car ovascular affections. I.. Xa~ola? 1103M Of viruses froze the blood. of patients with cardie- ~~gr,~MIYA,rYr~~ vascula - *ffeattioni. Sto cero.,c inframicrobiol., 1961 12 m 3. 275. For the first time in specialty literature,, the authors describe the isolation of three viral agents from-the-blood ?_ of patients with various cardiovascular affections (Reymaud's syndromes throabo. angiitis obliterans, cardiac infarct) _~ _.. . The isolated strains presented common characters, eial ? tar to those of the Rickettsia and Farariakettsia, demonstra.. ted by cultivation in the yolk sac of eabryonated eggs: patho- genicity for the white mouse and guinea-pigo largo size and stainability by the Giemsa or Macchiaveilo method'* The-serologic investigations showed that two ssitrains_eg- hibited en.:.. antigenic structure common to the or. nithosis vi- ruses# and the third strain was closer to .R.prowazekj. 6270- D,SARATSANV' f '? ;, g G.SORODs , B.ANAGNOSTE, D..DUMA i M.MARTA, V.CUPSA: Investigations on an ornithogiss ,pf, zootic in canaries., accompanied by human cases. 8t, cere, iiaframicrobiol* * 1961, , 3, 347. .. - - ?- Fr+.+.ri v 1;y V aalYCL.l as i ,.,a During an epiaootie among canaries (Serinus canaria)9_a virus was isolated which was listed,, in view of its biologic, morphologic and werologi? characters, in the group of avian ornithosist viruses. The isolated virus way also pathogenic for the siskin (Frigella carduelis spinuc), goldfingh (Carduelis elegans)i_.--. greenfinch (Chlorin ohlori chlori #),. linnet (Carduel- IM s anna- bina) and giail (Ceturnia aoturnix). The three caretakers and the other canaries, contracted the disease following contact with the sick birds. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 ? 386 c' 628.- MARATRABU , G.BORODOC s CAURDAR, B,PU -ANAGNOSTZ. Bovine ornithoais bronchopneum nia auad the,,poasihil &.r.- ties. of humus eontaminatigmoSt. care* inframicrobiol. q 19619 O 39353 From two fool of ensootic pneumonia; of CaiYesg 2 Vi- rue strains were isolated which were listed, in view, of their biologic 9morphologic and serologic. charaoters: ,. among the, ornithosia viruses belonging to the Parapiokr ttsia group. gd 1ogic investigations revealed the presence of anti- ornithosis antibodies in 31..7 per cent of the caly 8 that bad been sick and in 44 percent of th+atsrBe The presence of positive reacctionS aso ag lien leis: the .. . authors- to . raise the question of co xeider g enZootlc pne o nia of aa3vee as an anthropozoo*oeis. 629. ?oBARATRAU 0 C,St3RDAN 9 Gc.SoROD4t W 4 GN OST2.0; A . 3ERCAit o RI,RnSTFLe Inveeti tic son:. +r ett "ia ...abortion of atu the a erisenta dies ,s? d atte~r is at. active unization) a t? cove.* infrau.ero a .dl*91961, 12. 4. 4410 _ Investigation of the pathogenic aetiVity for `gta t - sweet of a pararieketteial etraiaio isolated frogs -ep abc 1ss showed- that it induced a minor disease followed by bo'rtio o ** premature . birth of.non-vvl able lambs and pneunoemteriti6 of? -. the.. newborn lambs: --Premature births or. abortions; were preceded p?s f partyam a+~ by-prod oral. signs -and- followed by ante--and cidents. The experimentally infected ewes presented iOn t0a wela snd.1-mphocytosis l, as well as wires a, consta l o ' 00- 3o days.. Complement fixing antibodies . were found at' me ewes 7 to 21 days after the infection,,, and. in the IOU, after sucking the colostrum for the first tim?r. w a. Pararickettsias were isolated from the abortus xga -B t. the foetal membranes and the lochia,. - -- Active imaunizations which could not be owe: vows. a clallenge inoculation of 175.9006 nice W was sbtainedi i - - - - 50 experiments with a o of per cent formolated alusin u* o' ' Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 387 adsorbed vaccine ..and a silvered, vaccine,;. 6 to 20 days after vac nation, 'the. owes presented complement fixing titers of lj8 am l/64. . _.. th n e mash or their researchesithe authors deriohstra., ? to the i ' spec fics oi pararickettsiae in the etiology of enn zootie abortion of ewes. 63o O.SURDAR, D BAR TFANTJ, G BORODQQ 1'?Al'RArTASIU, B,AHAGNGB? .1 am , I .B RCAN, a jRSspp . liver' mental study on . arCL U ket Isis . Pneumonia of calves. cars . inframicrobiol . 9 1961. 12 _ 1i _ Aar _ e authors describe the results obtained in e xperimen, tal infection of calves with a, pararickettsial strain isolated by then also from calves 'and maintained in the iI ;bar: . serial passages in mice'. And . embryonated eggs. The calves. devo lop a febrile catarrhal disease accompanied bya relapsing neu? me?emterie syndrome, stagnation of growth and progres?ive debi- lity. From the hewatologij viewpoint le ukopshim and 1 ymphomo.._ nooytosia were found. Anatoao-pathologicallY,, infiltrative' lym.. Phoeytlc broneho??pmeuaonia, proliferative lesions and haea:or-rh go in the lymph nodes and spleen, hepatitis, intestinal and _ parenchymatous nephritis and proliferative endothelitis were.... found..Bleaentary bodies. were revealed in the tracheo?bronchic epithelium; alveolar endothelium, in the epi=thelium of the tu? bull contorts, the -macrophages .of the spleen and liver and..the bl?.od..leucocytes... V remi,a lasts more than 4o days. In a..s3.n le mass was there _ a- gran- gram. negative -. uoccobacillus isolated., from. the lung;_it_ssriousl y aggravated the-evolution-of the, disease. giaet e_>sero~;ogic iriveatigatioas showed that..:onlT__in, a-? single calf werecomPleae nt.. fix ing-.antiboc ee, found after- grok Mob begi.aming. of the infection -The results ., obtained.... showed that,the atiologim::agent-of bronch6pnei omia and-eete? tisybjf . galves is a ' pararickettsia ident'ioa to the ornitho.. sis virus. `r j[?? ^ T'? Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 63l, AQADeSTsSJIICOLAU, C?SURD4N, D.SABATBANU, P..&THANASIUa GsSQRODOC, B.ANAGNOSTE,, C4ILIESCU, B.RADESCU. Viral, et olo in the sphere of card ovaacular affections;;. II 'Viral . stud offif casse_B,, St. cero. inframisro- biol.e1961, 4, 523 The author* dewribe the results obtained in their in- vestigstions on 5o patients with various cardiovascular di- seases. Forty one of these 5a patients presented a positive serologic reaotion to riekettsial and pararickettsial anti-, gene, the highest proportion being found to R.burneti (56.97%). From these patients, 24.straine were isoleLted, 15 of which be- longed serologically to R.bnrneti, 3,to R..prowazeki, 1 to R. mgosert, 12 to the pararicketteias (Q,18), of which 2 both to R,burut ti and to pararicketteia Q- .8? .-The-isolated agents were cultivated in white mice and in t b# yolk sac of eabryomated eggs. Intraperitoneal inocula- tion~ to guinea pigs induced the appearance of serum antibo- dies, _ The histopathologin examination showed the presence,, in th.-damaged-vessels,, of elementary bodies stainable by the l[a~ :`~ ?i_avello method. Antibiotics from the tetracyclin group exerted favoura?? bie. action in the cgses studied and brought about negative, serologi4 reactions. 632,- AcAD.S'F.d.AICOt,AU, D.SARATRANrJ, C,.SU DANA PATH &NA81U, B.OAGNOSTE, G.dSOBODOC a C.II,IESCU, R.RADSBCU, Eolis ant;t'odie~s `;ri,werkwr~. an tne zootechn_ice .'fi_eld.:: $t, ce'rc. nfr" crobl`al.o 1961, 12, suppi ., 365t Rev.?Bc- ti i...jted. 19E1; 6,..` 1?2', 105o By ?. performing complement fixation tests with 344 sera..... from workers in the zoctechnicai' field or in slaughter=house? the authors have recorded. a mean index of positive ornithosis reactions amounting to 4 ,':$ g 3.. per cent, The`. highest ind ces.'Were recorded in breeders of psi. tacine?. (72.7 per cent), pi eons ? e g (58?5 Per sent) and palmipe.D. des (490.o5 per cent). The authors also emphasize the impo>rtan' ce.of mammals as sources of infection in mans a fact resulting from.. the,, high serum positive indices recorded in sl:aughtei . house- workers. and in those. employed in, the department of an- ti=amine-plague products 4o .,. . per cent)e .. .. -.-..The -.widespread oceurrence of infections due to avian and--mammalian ornithosis gtraina belonging to the Parar cket. tsia group is poin 641 C .SURDAN B . D.SARATJAJU, G.8OR000C B ,FUBRER..ANAGNOS Ca' . +yoas? iv' -G L50JLation or a virus. belonging to the rickettsia group frQa an enzogtii of aranulous vulvoon. vag nitis,in, cattle 8t?.oere, inframicrobi9 ..,{\ 1961, 129- 'auppl. s 373; Nev.sci.lied.1961?6? 1-21 . p 127? From the vaginal scrapings of cows suffering from cons tagious granulous vag.nitis a virus was isolated in egg ciil. tures, which proved tube pathogenic for the white mouse. The LDS in mice is of 16 `5o The virus Isolated belongs to the group of large viru. sea;. it is polymorphous, multiplies in the shape of antra and extracellular col,onies,. and stains by Uacchiavelle4 - - Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06 : CIA-RDP80TOO246AO19200640001-8 method. A I/i? dilution of ornithosis serum neutralizes 700 3 5e in mice. The biological,, morphological and serological charae.- ters of the virus .solited demonstrate its relation to the ornithosis viruseso 642 e- I oBULOGBA, i O iOB' NFELD, I.STRATI , De5ARATNAI U... _ nithos38 3nfe tion in i radia@ some data Ronctrnll. ing. ted and in non- Irradiated whits ?mieee St, certe in- f robiole, 1961, 12, suppla, 3819 By irradiating whito .nice with a single dosi$ of 3oo-39o r the susceptibility to experimental infection with e ornithosis virus was increased.o - - Following irradiation, ornithosic infection could be induced by routes by which no infection 1s normally caused in the case,of certain strains' CARUNTU, L0PAUL, AOTIUGRBA. Histo aQ y 643GoIiARI1+NB(:U9 Fm th oldie aspects in a case of -re a aced typhvus f ear b Report read at the session of the Institute of Infra microbiology, November 209 1964. -- . -~ ? _~ A ca?e of typhous fever, repeated after 36 yearsy Is deseribede The histologic lesions were predominant at the level-of the -heart and. spleen of particular intensity in -the retiou- lo-endethe lial..system and _ c onnectivT tissues probably linked to the latent action of rickettsias'a' Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 X _. Vari a 644-722 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 6440- 20 ^7OLAU,. R,PORTGC ti?,T,.&.q eCAJ41 0 Staining and mores, of the mumps virus obtained by paro t 4 u;q- Recueii d1dtudes m4dicales? Bucarest.1949?189? In the fluid obtained by parotid puncture from 12 muunp-n patients, It was possible to reveals by means of the. Moron v atai.mi:ng method or by that of Nicolau with phenica ted isamins o elementary coccifo bodies with an approximaa. to d . eter of 12a T 0 These corpuseular formations of equal- olse are to be found on the smears grouped ih ciusterse On the smears made with normal parotid secretion , - oorpuoc,lar for.tions-, may be found, but much more seldom9 of vari. blo sizes. These may e :ceptionnal.ly represent b: Lo-. phy c Ingramiorobes or, ratbor.normal oytoplaamjc. granules, whin4 cannot be taken for mumps elementary bodies, inclusions in human salivary cellic. ffinmps patients ~ and. normal rso o-.., Bul. Stiin4?Acad?R,P.R. Secto tonedo,195o ? 2?, 50 411, ACj ' p o , a T O COLAUO R.POR?OCA3& O L?CAJ'ALO Pmto2lasmle In the cytoplasm of the cells found.. in the saliva of muope patientsq it was possible to reveals by means of .the modified. Mann staffing method, the inclusions described by. 8ohier and. Jaulmese The size of these spherical or oval in- clusions ranges between 2-5/1 0 As a rule ? they stain red',- sometimes sometimes light blue or a shade intermediary between red and, be they are surrounded by a more or less accentuated halo, These formations are to be found in be per cent of the mumps cases? A comparative study of the cells found in the saliva. and-of the epithelial, cells, soaped from the mouth epithe~ la.um in normal personae revealed the presence of these for- mationa in 20 out of the 21 persons under observatione- The high percentage of these inclusions in normal pore aonsg. definitely leads to the conclusion that the inclusions found in the saliva cells of mumps patients and described--- by 8ohier and Jaulmes as path j; ;4orr epidemic mumps, are Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 , ,e, spontaneous Q? inclusions produced by the viruses that exist . in a latent state in the mouth of healthy persons'. ' M tIED1 s I.SAMUhh, T.SANDUL!SCU. Three mumps virus._ ._ . et_aiaa, isolated from the cerebromepimaa. f1cid of . epide mic mumps patients. Comunic#Wile Acad.R.P.8. 19559 199 1385. Three mumps virus attain were isolated from the sere- brospinal fluid--of children suffering from epidemic cps,. Two of the children exhibited clibigally apparent. neningeal . forms and the =third 'a cytologic meningitis. The . strains iso? lated from the patients with _meningeal ,forms.' yielded from,,., .. . the first passage positive hemaggintinations; the strain.sod. lated from the?~patient' with cytologic meningitis , acquired weak hemagglutinating properties at the second passaged Stress is; laid on invo1vement of the nervous, :system. in mumps,, which can onqur before -.the appearance, of the cease, sometieesithout any.olinical sign, of meningitis, Complement fixation .in. the .diagnosis Of muape, ? St.cere 0 647. 1, ' UEL , I.FRINDVAN,, E.WIEGL R * a T.BANDUIISCU. , inframicrobiol., ticrobiol., parazitol., 1957, It 387. Antigen'V fdt complement fixation~.was prepared from.. the -allantoid fluid=' infected with, ,the autoohtonoua strain,- ._ :._..Tests_m de..'with.this -antigen on 135 Sera in.complement fixation,, demonstrated the specificity of the antigen and the utility of the test -rin- the.. diagnosis of mumps. The au- thorn considered as positive ` f?i the, antigen prepared by then a titer of 1/16 for a single test and at least a four- fold increase for two tests, on condition that the fire, serum should be taken in the first days of the disease. - Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 348 648 To lZDb , ZSA LO Fm EGLLR9 T.SA JL!SCUo Comparat ,ve value of serologic tests in St, care*., . infrjmicrobiol. , microbiol., parazitol., 19579:89 4v 571. A comparative study was carried out on the value of. compler lent fixation (C0F0) and hemagglutinationa-inhibition- . (~,A0X) on 4o sera from 24 patients or convalescents suffe- ring from mumps and 2 normal subjects without mumps in their past history. Parallel values were found betwe?n the titers. of-the two tests, F'arlier more characteristic and higher ti-r tors are obtained with C.F. Following these investigations C.F. is considers, as sufficient and more indicated for the serologic diagnosis of mumps. The amylasuria and.. amylas@mia tests likewise constitute a useful and early indication'. 9.oo 1oSAMU Lo.Isolation of the mumps virus. from- the care.. A itu v of strait, 8t. ? cerc., infra bro-spinal fluid braes microbiol,emicrobiolo, parazitol,, 1958, 2, 19 51e~ Four mumps virus strains' were isolated by inoculation of the cerebrospinal fluid drawn from mumps patients, with or without mgningeal signs, into the amniotic cavity of chick embryos Two strains (SI and GN) were adapted to the allanto, is t uid. and to the anterior camera oeuli of the guinea pi'4-. - -. The strains differed from the viewpoint of their adaptation capacity-and-with regard to the correlation between the i.nfec- ti.ous-and~hemagglutinating- titer, During storage. of . the.-Vim r a_ decrease of-the -infectious titer is observed.;-.the more _aceentuated. the _ less. adapted....is . the strain, Strain G29 adapted to the allantoic fluid,, was used for the preparation of an antigen for complement fixation. The advantages obtained by isolation of.the mumps vi a, from the cerebro=spinal fluid are showme lending - support to,the theories that sustain the neurotropism of tdh .t virus o Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 The resen . of :aacifio, 611 e taz c ozeugclew il 6,50-o- ACAdoSTO SJHICOLAUA? N0CAJALs McSEPEANU GH,MARINESCU. tears o rua:us ~ amd .imi~reasio af:';2'iatec~lick8 s Works of the General" iest fic- Session of the Rol',R. Academy., June. 2 32 1950 1339 ? ::. Until 195o the morphology of the measles virus had been very little studied and.the results obtained were both uncertain and contradictoryo:T'he measles virus was revealed on mucus and tear snare and ea the - impressions of Filatov Koplick"s enanthema9 stained with phenicated iaamine.a The virus appears in the form of cocciform elementary bodies.' grouped in clusters,. measuring approximately 12o T. The frequency. of measles elementary particles is higher in the. first hours after the onset of the disease p particularly in the mucus and. teara.9 gradually decreasing after 48 hourse enanthema -ih"`meas.es7patieftB 65-le- N?CONSTANTINESCU, M.IWCJ., E0DUGAg AOVITAO , Investigate tions on the transmission of the. - virtze' of r i ...a ea the virus from the blond. Le B ,. to - rabbi.lGS" IMd-_"228 of 1 e ` C tom~ OfIthe man a,r.n .em+....a^ mrsia..sa~ r~r?rn s yir971$`~ in ebb flat8t ".,eB'~$a^8t?- Cer6:;' Infrai 1crobiola, zaicrcbiolm a ito1 3 2 o~~ ~. 95 ~`~e,3 a 37 The virus of German measles was r veale,d in the blood of patients by inoculation to rabbits as nd guinea pigs only In the first three dais .of. the disease. never lfter& In the , ;l o a blood-leukopenia syndrome was induced with- appea- ace of plasma-cells,, accompanied` sometimes by variable fe? brile reactions, .The virus .cultivated ovvo in the yolk sac gave rise in the rabbit to : thee; sa ma""b1*sd syndrome a? -- Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 4so 0" o ' ~S~a~ aet~ fl R I . , _70MICU p eaEP -ICA, Oo ThOIU? Narvous man ,festatioV in measles. A tdmo= tF.ol e is stt d of aor - it .,ous men Dance hal ,t ,s Sto ce o inframicroblolo ? ticrobioi; paraz.taiO 19)2 2fl 32) 1190 Thr cerebral localization is the most frequent news nV a gc station" in the courage of rn as1es, Rumanian me tal literatu has not let published a more nportant work on the hiet?pAthology of cerebral omplicatio in ffie s1eaq Thy present paper deco; ,bea the histopatholog;ig s@peet? Oncon tovod, in 15 caags of mOas1e6 with lethal nervous manic fe~tst,o e clawi a1 picture of dom linizing encephalomyelitis with Glia1 proliferation wag Once tere .in a single eansi' The otboW 14 cases: we characterized.by the presence of a gem rou> arnin,oaeaeephalitia process of vable intensi; eid;.. tbelit`t ulth v? us preva1enee9 blood stasis with.leukoc a ;. throb r' Q vascular dilatation and stension of the perjVgA. c u.189 Rpsi;20S occupied by a serosityo These cases may be listed t supraacute toxic type of aeaslos described by 653o- G0 I SCad O P oaTROE8Ca?1 ? N, a nervous i dons in the course -,of mo bbs.' liml s ., as et -..Ste e d .., i 'ra is abiol0 0 _. m4crob 01,0 ?p a~ tt~l. 9 19520 ervous accidents in the coarse of seaales seldom encotan- tered.-a have been described in isolated cases in oar .country, The clinical study of 31 cases with nervous complication out of a group of 2637 cases of measlesg hospitalized in the children? s war for contagious diseases (Dr0A.Stroe) p during the 1945 1951 period is presentedo The av6tage inc ite of thanno accidienta (1015 per cent) is *lose to that found in po 1.ioD litio with slight a nual-dif arences ranging between .o44 per cent 1945=195? and 2,elg per cent in 19510 Nervous complicationo usually appear between the 3rd Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 .401 and the 7th day after the bogi, it of thc) ak athemae According to the clinical form, thc: e erGxs 2o caseb ene;?phaliti??9 meni eacephalitia , l raae i ;?me ph J litiss 1 serous meningitis* Theme neurologic nattifestatio were associated in over half of the cases, with other coplications (interstitial oV bacterial broncholpa eiioniag otlti ? etc o The mortality rate -in the cases with; nervous aempliqaticn~ wan 46 per cent; as ciatizn with pulmonary co!.plication of a viral or bacterial nature increased it ~almos tvafol1. (';.3o3 per canto)* 65 ? AC A~ O ~`1' o 0 3 tai kU 0 CQ'~1.^-"~'AI~TI 1ESC J ~l v' 3 . m ; lei Po ??l sox;a of t ?^ rUPI, of o lea: i VV IS arobi;ole~q mla o1 iw s o ptLt ?al1twt o ?. l9 ? I? 3 , l 9? sine a, Ito wi, _ pea c ti ato im to the org im by the aaopp',ja. .b X'DU+-:0 p a Rs ? at't > pt bay m t block fir OIL of GI nta? by ractiec. OZ Pa0e v + 1"+1 +1'n'i.z:.t?oZpp wit, a 5 3 to i.:, fecctione k btitr 1@g, 11_ imi nizationo The virus attenuated by *on- waleacent seg.v.,;aa it a P po:r is of l voia o virulent blo64 + 99 volumes ae Y01 (kopt in contact . dart cg 1 hour at ?C, .. then 2 hour at 4400) was administered by nua it in t ]1=,y:tia~:: to a group of 27 ivh: drez vdthoUt o?cbilii a?'. tece enta 0 . ataoa g which a case of measles 'had -appe edo in -the fol.loving 3o da as o ? no otter cane ,% reported e Passive local i umizatiom Convaiea ent aye wane in- stilled tr1co &..' . du t1 ' 4.. d`,8 (71 p in aeoh nostril) to a group of 12 children, ged 1.4 ra a so, without aea le s is their a ar%?tacc d: Mt- s:0 In a cc o , w aQ, im which a Cl se; of sa confounded with thought (conacienoeand: that our scientific, knowledge of the brain Ades not .justify the inclusion of conscience in the field of natural sciences. By the study of this article to-dayq the authors open; up prospects for consi=dering Victor Babe$ from a :new angle,,. that of the history of riaterialist philosophy :in -our..oountryr,, thus situating the man of science among the first materislist thinking inteliestna1s of the Rumanian people. 69o o- ACADoST oS.0NICOLAUa ECATAIro. a pparent g . enzoo tic dise ase> in rabbits -due-to as 'Yunltno `fie o" Bul.." St, ACad0R.P:e ar maf. " rabbits, reveale:4 in 45 cases ;(normal dr previous1y,A 0 -' culated animals) the presence ofchronic :lesions characterize by lymph nodules and perivaecularitie processes, with :-rononUe clears.? The most interesting lesions were encountered at the inocu.iatea win various, viruses Cana '! } anon- nocu.Latea-, TV cr A study of lung sections from .loo " rabbits,, 5? of wh Beet; -Ste-Redm 1958,,"? "' ".? -5"? . . It was not possible to detect "nuclear inclusions; o# Bible nicro-organisus in any;of the sections-6, level of the bronchioli or- :around the blood veeselsd The animals with these :lesions behaved perfectly 1nocmahl`., not presenting any morbid .syri tox .. Study of chronic pulmonary lesions in a group of rabiitip in wbiicb. the leukocyte count had .been performed,, showed a aer* tain parallelism between the :;presence of these lesions-and the: existence of monnucleoeis,9.'particularly .lymphocytoeise These data.gave rise .to the .question if a certain rela- tionship does not exist between blood lymphooytosis and pulmo- nary lesions. The growth of these lesionso, proodf _of the apparent d1 cease, eliminates any errofg .. of interpretation in the. ceur of experiments on rabbitse, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 r ,,)l. 3i.1; 1 ch r _tg iz"ng hboj, ___ .__? 'o ki oAC dO P0PO30 nic h ~.(1db Ra~~j~o~l la layer. JBL ~., Iayer~0 a(?i Pkonen /~~.s~~.pr'`~~^. ~la. of de/'^~ -A der ari- to F:S aipig?. i 0 r rt._, 1 ep is i.iu _ appea 'fl ao 'ok?lpa li^4. `9 infi it ~'~d` ~1 c1 of leu. ^ r P_.,Lz pa's't cularliL.,y pot 'ug1clearoo r A- C tio~~ I J C'wi! a al-9 fo[?.(~d artnai esr i eti'ree intraaeptt. elial g"ie o pustleaa tttw, e bec.on4 confluent aLe, constitute the eruptive elenQ:r t d'est:roya the co muoua epi -; e .ia 1&7,6r. that ee- parPater i. from the exterior, 'in the skin lesion duce to the virus of in ' Mile Sueta Cilia ciao cytoplasmic inclusic+ra are to be fou t in the epi= N.; ~+ay. ! cello, Viral colonies or pre-inclusion " stages ((p ,abahly motor, leer, du?(,,@ nora-teda as glutinated gerc ) nay a i ;t in the Malpigbi layer and, in the `:filtration le ooyteea r,;` rticul R-r17 in the 14) non, w1clQa , cells, The latter in= ^,,:a ^~~topl~~ naic fo ritlticns may als? be found in the infiltra- _'z"u'l Cc- ..a l the Amsrte, The enGa ble of the lesions induced. in the skin by the }w~t,a T?trs'u o because only a virus can produce such Inc lusion = 'Uffere from. t .e changes produced in the s .in by the vaacciniap) fix-;:-pee zoJt?org si!ali pox or herpes vi .seso So ASE CC v Econojjaic use Of food. Lo `i> a.l . Rev. Sto Med09l.951, J uary96oo In t %,a -'pod of laboratory animals oats are one of the It is known that at leas`' 50 of the grains ;;:,;,~,n to ter;' aalo is scattaredo nixed with the defections and It in prc-pomed to for i a paste with the grains a giving za rod-like ,shapes Which is dessicated and then cut into.. lf, pieces and given to the aninals. The animals can gnaw ;?F foot up to the last bit The authors propose to include into this paste Qrushed and. thole oat grains fl wheat bran and water, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 t,ryU~F r+ha{;yp~f~ r~8doa(w;;A k'?_ "Cu }~yE01 S8 yo~t9 2s 9?ppn `gyt Ica Ca~t.)},$ ;.rte al.`?. d" Gs,.d~ L. rrho'~lA oo.. M'm !,i t' t" s, now-born, infant), St. cg W' y > +a riicrobio1o9 parazito1n, ~b 9393= 'dM..%~0 Investigations carried out in. 22 cases of c~pi?e~.i 4..om r; nox rf in infants (epidemic diarrhea of the +e,a-`bo n tc .~la er t rate ~ the presence of the influenza vl.ruo t o ?., r l;l n-Hsu-pharyngeal washings (7 cases) and the f .ec e (8 c a ) o . 1--t was possible to isolate the virus fro,-- 9 cascs in is ,9 :tf `sue c LGrl z!_ -1.Ji1 _itd, ,n ",:,~j local :~.Q'i. tiJ'C?F,~a, cb`.',Gr,v,,- d. be 4 v,- co s b'L a'! -it k' ? e & ~' cab "A ty o~ :~o f_aS y' ,bra= .15 ..e all tr'.',~ C-3.3-8. ex except G"~' acid e of t 23 .1 cf tin L g T FF~y q~y. e ty T'srd a C 'L~ .1)e R y. * it xr ie' ~3?i ~:; l~' c) '' p espc i.iy in t ho 'c: ?7 Za 3 i. of a jud r"Ib', (:- r with a v t?'..C 4!.t".'fwn ci;; of th rg t a: ;`'c protection of the ree:1?"voaas zyo,tai:.1 a airiot .= ' Cr;' ', : 5e5F'..o Of fructac-'21 a^ ti`:~ ~ y Report read at the Institute of -e~s"~L ~ of the .o-_'PORO eca er s~ 5 October 1959 'Ic?ohc].: , {ant -ho. f Z, was prep 4. 'n..~ for GY .l b l..i arF ', ester.. a r.tb phosphoric a~_l P11' a:::llvi m .;ion of e;zy es0 determination km(' jI.J of .i.xlpor W arc e in the o-P : - a,:", test - in the c ours e o is of partiou.1a ' inn.ere ' ..n Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 - 428 - clinics of infectious diseases. Synthesis of barium 1,6 - fructose diphosphate was obtained by direct esterification of fructose under the action of specific enzymes,obtained form brewer's yeast;thus inversion of cane sugar and phosphorylation of the resulting dex- trose is avoided. Ezterificetion was done with a disodic phosphate mixture (0.25 m) and monopotassic phosphate (o.1 ni) at 37?C,up to com- plete binding of all the phosphorus available (3 hrs); the ester is sterilized with barium salt (acetate). The active value of the preparation was determined by a method proposed by the authors,using as standard,"standard." fructose diphosphate and as specific tests aldolase catalysis and 2 - 4 din; tro--phenyl-hydrazin staining. The indirect method for the determination of fructose -1, 6-phosphate cannot be applied to the biologic product, because of its content in pri- mary esters(phosphates) This synthesis procedure guarantees an output of 33% cal- culated in fructose pure barium diphosphate; from cane sugar only lo,8 % is obtained. 7o3.- R.PORTOCALA, I.SAMUEL,G.TIRNOVEANU. On the Presence of cytomegalic inclusions in the sub-maxillary g ands of the new-born infant. Report read at the Second Congress of Hungarian Microbiologists ,Budapost, 22 Sept .1959. The presence of intranuclear cytomegalic inclusions was observed within the strongly hypertrophied cells of the sub- maxillary glands in 4 out of 32 new-born infants, that died from various affections. The ration of positive cases in this study is l2*?%.which egrees with the results obtained by other authors. This paper purposas to report for the first time the pre- sence in our country of the disease with cytomegalic inclusions in new - born infants. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 vae4 7o40= IoADERCA0.Recent investigations on moasiesvirus.,. :Jcrobiolo,, parazitol.os epidemioloa 1959, 4, 6, 481. The author reviews the data in literature concerning the isolation and cultivation of the causal agent of measles in emhryonated eggs, animals (monkey) and especially cell cultures in vitro. The works of Endei gMilovanovio, Melnick and Ruckle regarding the susceptibility of various types of cell cul- tures and the characters of the cytopathic effect produced by the measles virus in these cultures, are described... The author likewise reviews the main works dealing Cp4,ith serologic tests in measles, especially complement fit. . xatioa using as antigen the measles virus grown in cell cul- tures. 705?? ID08ARATEA19Uo borata i nom of infectious mono- nucleosis oMicrobiolo, parazitol., epidemiolo, 1959, 4,. 2, 1750 _ _ . _ ., -. _ .__..,The laboratory methods applied for the serologic diag= nosis of infectious mononucleosis are described, _as well as the presumptive tests, and the quantitative and qualitative methods currently used. Appraisal and interpretatior of the serologic rebults is another aspect discussed. 7o6?? G0BALMUS, I0SAMU!L, LoMIRZA, T.BNACHEO Investigations on a severe epizootic due to paracolobactrui. in suck- li.laboratory rats. Microbio1m, parazitol?, epidemiolo 1960, 5, 445; Acta Virolo9, Ung. 196o,.2,, 2, 1870 The authors describe the symptoms, histo~)athological. lesions, pathogenesis and treatment of a spontaneous, seve- re epizootic ea Used by Paracolobactrum intermedium in sucling laboratory ratsm - Since the paraoolobactrus group with the exception of the-Arizona and Ballerup-Bethe..a types are considered to be nonpathogenic, or very slightly pathogenic, it may be Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 assumed that the cold endu:r?' '.. 7,t- the anima] s during transport and their young age interfered in the production mechanism of tie infectiono These factors promote changes k the animal's reactivity, permitting the development of the virulence of the virus, which passed.beyond the intestinal barrier and caused septicemia infection, Certain sulphonamides.:: and antibiotics of the tetracy- cline group, administered in the food,of the animals, brought the epizootic to an end in a fear days, 7o7,- TOHUNG, N.,DRAGA SCU, Experimental investigations in Molluscum contagiosum. St0 cere,infram.icrebiol0, 1960, 119 2, 2930 Inoculation of a non-filtered suspension of Molluscum contagiosun into the chorio?allantoic men,. cane of 9 days em- bryonated eggs, induced microscopic proliferation and intense stratification of ectodermal cells, with an aspect of invagi= nation into the mesoderm. .laF~ ....._Intens?_bistiocytic proliferative lesions and slight phomononuelear. l_nfiltrations, are observed in the mesod.ern Within the cytoplasm of the ectodermal cells, the pre- sence of eosiiiophil formations surrounded by a halo and varied in number and.. size, is noted0; Hire and there these formations present a non-homogenous aspect0 _h.- ___Intracerebral .inoculation to-250-300 gm young rabbits induces,. only_at.. the first . passage, inflammatory. lesions in-.the choroid plexus and-the wall of.the 3rd ventricle. Within the neurons of the cerebral parenc#Y.xa, the presence of granular clusters is observed, suggesting the aspect of elementary bo- dies that push the cellular nucloqu towards the periphery0 { Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 431 7o8o,. DT$T,SoBICOLIU, R.PORTOCA.L&, Recent Soviet investiga_.. tions in the field of inframicrobiolo. St, cerc.infra- microbiol., 196o, 11. 29 317. The present paper is a synthesis of the main Soviet achievements in inframicrobiology. After expounding the evo- lutioxa of inframicrobiology in the Soviet Union, the authors. briefly describe investigations in poliomyelitis, encephali, tie, epidemic hepatitis and influenza, viral diseases to which. Soviet discoveries have brought important theoretical and prac- tical contributions, such as the results obtained in antipo- liomyelitis and antiinfluenza vaccination, histopathologic. .,nd experimental observations in epidemic hepatitis and ence- p:kr~litiso 7o9.- G.UANESCU-POPESCU. The action of vitamine C on the c l-p tivvaation of certain viruses j4.4hick. embryonated eggso St. care, inframicrobiol., 196o, 11, 3, 4ol. A series of investigations were carried out concerning the influence of vitamin"."Con virus cultures (influenza, vac- ciz?ia, avian.diphteria-variola, mumps and avian pseudo-pest viruses in chick embryonated eggs. - -? . The results of the experiments showed that vitamin-0 in 00005 gm amounts favours multiplication of the viruses st...'ied in the chick embryonated egg, while in smaller dose it prevent s.to_a.certain extent development of the vaccinia and avian diphtero variola.viruseso The favourable action of Na ascorbinate on virus multi, plication suggests its eventual use in the preparation of vi- ral antigens, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 432 ? 71.o0- ACAD.ST.SoICOLAUo k e v ,~.L zed vaccii?.:s, A new method. for the reparation of attenuated vaccines ,_against ra- bies. tick borne encephalitisQ,m herpes influenza and - ornithosiso $to cerc, inframicrobiolo, 196o, ll,, 4, 489; Arch, fo expo vet0Medo 1961, A~A 349. Our metallized vaccines, obtained with heavy metal ions,open up we beleive a new-road to the preparation of attenuated antiviral vaccines. The immunogenic activity of the antirabies vaccine pre- pared with 1e5%lgooo sIPI.-ver nitrate is very intense, both in. laboratory mice and rabbits. By.elimination of the excess me- tallic ions from the metallized vaccine, an. antirabies vao- cine was obtained more active thant that of Fermi,, and which maintains its ectivity during at least 3 months. The virus, in the 11 silvered 11 antirabies vaccine is submitted to an irreversible'attenuationo _-- This-same ,vaccine, prepared with the radioactive silver slat (Ag11oN03)"Permits study of the migration of the virus vaccine in the organism of the animal and of the mechanism of tissular immunization characterizing viral diseases - The tick-borne spr simmer. enc. halltis virus in the . brain triturate obtained from mice which died of the experi- mental disease0 suspended in a lo5:1900. AgNO3 solution and. maintained. 44 hours at 37?09 is transformed into an anti-en- cephalitis_vaccine which immunizes mice against the homolo- gous virus. The metallized anti-encephalitis vaccine is much more efficient than the formolised tracc ine o Comparing the immunogenic activity of the fz;esh formoli~- sed vaccine with that of the silvered vaccine 9 stored even .120.- days at +44?C, the evident superiority of the latter may be-seefl' We were able to demonstrate the powerful immunizing acs' . tion of the silvered antiherpesavaceine. This vaccine was pre- pared wither with 1/lo cerebral substance from mice which had.- died of experimental herpes encephalitis,, or with the fluid..of- in vitro cultures of the herpes virus on human embryo cellsoThe best concentration of silver salts for these vaccines wasog25s l,ooo silver nitrateoThe silvered vaccine prepared with in Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 433 vitro cultivated virus is more active than that prepared - from the cerebral substance of mice which died of experimen tal herpes encephalitis, The anti-influenza vaccine prepared from the.allantoic fluid of embryonated egg cultures and containing o.5:1,000 sil- ver nitrates administered by peritoneal, intramuscular, subcu- taneous or intranasal route, immunizes mice against the fresh. virus inoculated by intranasal route. However, the maximum ac- tivity of the silvered anti-inflienza vaccine is obtained when the animals are immunized by intraperitoneal route0 The silvered anti=ornithosis vaccine possesses a manifest immunogenic potential. The 3:1,000 or 4:.lflooo silvered vaccine prepared with lung suspension from mice which died of experi mental ornithosis, immunizes very well the mice to which it . was administered in 2 intramuscular injections or once by in- tranasal route.0This vaccine, more active than those prepared. :from virus. cultures on embryonated :eggs or from cultures ried_dut '.according to the Shindarov method (in vivo and..then_ in.vitro), is likewise much more efficient than the formolised vaccine.- Just as active as the silvered antirabies vaccine is the cered antirabies vaccine. The brain triturate, obtained from. a rabbit which died of fixed virus rabies, suspended in a o06:l,ooo copper nitrate Pr 0a5:1,000 copper chloride, be- comes a very active vaccine. The o05:1,000 copper chloride an- tirabies vaccine appears to be more active.than the o06:1,000 c.opper..ni.trate vaccine.0The latter, however, maintained, its immunizing properties for mice a longer periad of time. jllo. AoDEMICI, ALOPETRESCU, P.ATHARASLUo Associated anti, toxic, antimicrobial and antiviral vaccinations .(We- rimental.investi ations , St?cerc.inframicrobioie, 196o, 11, 4, 50- was observed that mice immunized Jtiki.J} a c;c::. iex d.i.ph= .It therodtota,.nusG.poliomyelit3.sminfluenza vaccine, present comple- te resistance to the challenge infection with the influenza or poliomyelitis virus and in a lesser degree to inocu= Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 -434,- lotion with diphtheria or tetanus toxin. Guinea pigs it unize'_ .. with the associated diphtheria?tetanus=pertussis-influen za vacei- no present complete resistance to inoculation with diphtheria or tetanus toxin and medium antiinfiuenza HAI antibod,Y7,-~ titers,, lower than those of guinea pigs immunized only with the anti- influenza vaccineo The experimental results confirm the findings of other authors on the slight antidiphthbria and antitetanic i. uniza=> tion of the mouse and the fact that serum antiinfluenza HAT. antibody titers do not tally with the degree of resistance to. virus inoculations therefore to the degree of immunity proper Vaccination with the complex diphthero-tetano-pertussis=influ?-n- za vaccine induces a good immunity against diphtheric and to- tanic toxin, poliomyelitis and influenza viruses, 7120= AcDEREVICI, C0SATMARI, ALoPETRESCU, P?ATHANASIU4 I0POPA,. CoHONDORo Associated antimicrobial and antiviral va ina- tion to volunteers o Serologic ctk 4 idemiologic , datao Kiorobiolo, parazitol.o, epidemiol?, 196o94,. 3230....- .... The authors present the serologic and epidemiologic re- sults of associated vaccination to volunteero, showing that the best results are obtained with simultaneous administration of TAB and antiinfluenza vaccines. The results are not so good. when the two vaccines are administered at different 'intervals, 713oa O0MITROIU, GOBALMUS, MoANDREESCU9 M0PQPA0 Serum aldolase modifications in rabbits with alloxanminduced diabetes. Conference on Hepatitis, October 2123, 196o? The investigations had in view appraisal of the,serum al dolase test in rabbits with alloxan=induced diabetes. The re= aalts obtained showed increased aldolasemia values, probably due to an abnormal acumulation following destruction and hyper- permeabilization of the cells damaged by alloxan, on the one hand, and, on the others the fact that aldolase is not used in the process of tissular glycolysiso Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 435 7140- AL.PETRESCU, P.sATHANASIU, S..CIRNARU. Hepatic morphologic lesions caused by a viral agent isolated from infants.. with an acute toxic respiratory Syndromeo Interregional. Conference on epidemic hepatitis. Sinaia, October 21-22, 196o. A description is given of the lesions induced by this viral agent at the level of the liver: diffuse and nodular infiltrations with lyympho-monocytea, hepatocytophage proces, ses, lesions of the he-atocytes, fatty charging of the proto- plasm and pycnotic alteration up to lysis of the nuclei. ?15..- N.CAJAL9.O0BURDUCEA, S..MATEESCU, G.WINESCU, M.CE?. PLEANU, Y.COPELOVICI0 The evolution of some experimental viral diseases (influenza poliomyelitis, Coxsackie, ra- bies, herpes) under the, action of radioactive phosphorus and iodine (P 2 and IM). Acta.Virole..Ung,.196o,.2,2,181; Stn cerc. inframicrobiolo, 1961, ..2, is 29; Excerpts Medica 19610 Sect@IV9 14, 12, 3516. Experiments with the influenza, Coxsackie, poliomyeli- tis9 rabies and herpes virus, in adult white mice exposed to the action of gamma and beta radiations emitted by p32 and 1131 showed that increase in the sensitivity of the animals to the pathogenic action of the viruses tested is obtained tinder the effect of these radiations: _Under.the.action..of...internal radiations, some viruses as_.for-.instance -poli3virus type II (L~F1) may realize the- experimental-infection also.. after_.inoculation by routes other than the selective one (peritoneal and subcutaneous). Moreo- ver, the Coxsackie virus (A2), non-pathogenous for the adult white *iuse, induces under the action of radioactive phospho- rus and iodine a high proportion of lethal paralytic cases. The results obtained are explained by lowering of the animal's resistance, a true " irradiation disease ", develop- ping due to diminution of serum properdine and cellular meta-. bolic alterations that favour the, mechanism of viral multipli- cation Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 436 The increase of the rad ? -9c tivity threshold in nature is-...-. assumed to ifluence morbidity from viral, diseases and the appea- rance of new and severe viral affectionso 716. R.PORTOCATA, I.BAMUEL, GoTIRNOVEANUe Presence of cytome- .. .ic inclusions in the submaxillary _l ds_,of the newborn. .cta Virol..Ung..1960, 2, 2, 188; Probleme de Morfopato- logie.1961, Ilnd vol., 265? In the submaxillary glands of 4 out of 32 new-born chiles..: dren who died of various affections, the presence of intranu-.- clear.cytomegalic inclusions was found within the hypertrophie cells. Their incidence is similar to that observed in other countries. Their presence points to the existence in our country of a disease with cytomegalic inclusions, that has been detec- ted until now only by histologic examinations? 717.c NoCAJAL, O.BURDUCEA, M?ABDREESCUo Sturm the value of serum ro erdine under the action ofradiophosphorus.. (P32) and radioiodine (Z) St. c erc .inframicrobiol. , 1961, l2, 3S 2970 Internal gamma and beta irradiation with P32 and 1131 brought about in the white rat an obvious decrease in serum properdine. This decr4ase occurs 24 hours after administration of the radioactive substances and reaches a maximum after 4 daysO ..._.._ _ _.._ ._ _-_ _..- ._- The experiments carried out also-demonstrated the role of properdine, in nonspecific antiviral resistanceo 718.= G.MARINESCU, Z.MARGARIT o . An adenoh eraller is form of infectious mononucleosis. Spitalu). 1961, 280o Infectious mononucleosis, owing to its pantropism, takes on a great number of clinical forms. An.extremely painful cli- nical form with adenopathy is described. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 - 437 719- G.o MARINESCU,AOMOSTRGESCU.? Ve iculo.aphthou sto~ati Contributions to the etiologic and clinical stu .Report read at the Society of Medical Sciences, Dedember 7,,1961, From an epidemic focus of vesiculous stomatitis in a.day :oursery, affecting 16 children, Coxsackie virus group B was. isolated and identified by histopathologic and serologic in- vestigations- 72o G0MARINESCU9 I,MICHNL On the value of the presence of giant cells in rhinophar-Meal smears for the early ... di nosis of measlesse, The 3rd Congress of the Czechoslo- vakian microbiologists on respiratory viral diseases,, May 1961, The frequency of giant cells in 1,798 rhinopharyngeal smears,, from l04 measles cases, was studied in various phases of the disease- The presence of giant cells was confirmed in 6o per cent of the cases towards the end of the incubation period;75 per cent in the invasion period and 58 per cent at the beginning of the eruption; it then progressively decreases to 9,4 per cent i.n the 4th day of the rash, with significantly altered cells, Giant. cells were found in a single case among the con-- ?trol group (33 healthy children or children with various ca- ,~?rhal rhinopharyngeal affections) o 72i - OoMITROIU, .C,BARBU, M0POPA, O.-SFERDEANU- Etiological and'serological investigations in the course of some., affectionsaaccopanie d.by rashes in children,, St9 care* inf ramicrobiol Q' ,1961,. 12, suppl o , 2719 Rev-ScUMed. 1961, 6., 1?2, 650 Etiological and serological investigations were carried out in 7 cases of atypical morbilliform eruptive fever, &t- tempts at virus isolation are.negative :: iUr, 3 passages, Serological findings, however, reveal an increase of comple-. ment=fixing adenovirus antibodieso These increases being evi- dence of.a recent infection,, they point to an adenoviral etiology, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 -.438 - 722o... G,MARINESCU, V,MABES. e., ,orphopathology of respirator viral diseases.; Symposium on respiratory viral diseases$ Baia Mare, September 29-30, 19610 The authors describe the morphopathologie aspects of all viral diseases involving the reapiratory.tract, stressing both the points in common and the differences, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 XI Monogr__aph? 1 v 18 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 440 1,? Recent Soviet investigations in the field of influenza. Ed,AcadLR.0P0R0, 1950, oL pages 5 tab., 25 bibliographic indications, A monograph reviewing 25 publications by. Soviet authors in the field of influenza published up to 1950, The bibliographic material is presented at the end of _ each chapter concerning" Im Etiology, pathogeny, experimental study, immunology and enidemioiog:v of influenza; II, The cli. nice of this affection; III* diagnostic methods; IVs prophylaxis and treatments 20o Recent ? oviet investigations in the field. of encepnelitia dee0 Ed?AcadjR,p,R0 1950, 78 page, 4 tab0, 20 bibiiograa phic indications. - A.monograph regarding speciality Soviet literature.up to 1950. A review is made of data concerning louping Sian encephalitis, Bielorussian encephalitis, Farms astern erg cephalitis, acute human encephalomyeliti8g Dialangar encepha- litis and equine encephalomyelitis in the U.S.S.R. In the chapter " Experimental studies on the problem of encephalitides " mention is made of the Soviet author R,M?.__ Schoen, who cultivated different encephalitis viruses in tumors The work of this author regarding the latent forms of St.Louis encephalitis is likewise cited, ACADe8to8,NICOLAU01.Inframicrobial hepatitid?so E ado ROPOR,, 1954, 76 Pga., 22plates, 14o referenceso - -- -. u -The . author, . on scientific bases , synthetises his own original conception on. the plurality of human hepatitides and hepatitis viruses a His modern conception is founded on the biologic transformation in naturef microbes and inframicro= best under the influence of new eflvjronjaental conditions arib sing due to the intervention of various factors, Thus, star ting from the classical "epidemic icterus ", identified as " Botkin's hepatitis q due to virus At which caused the great epidemics during the last two wars, the authors identifies Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 ~.4 fl 00- All It the sclerogenous hepatitis " due to virus C.and the " hepati- tis due to the non-hemagglutinating D virus "o The natural pro- cess of transformation of viruses cannot be considered as ended a3od it is still to be found in full progress, Next to the above hepatitides, the author mentions the variant due to virus B homologous serum hepatitis " or the " Sergheev and Tareev di- sease", casually transmitted by contaminated serum or plasma. The character. of each of these types of hepatitis are analysed on the basis of the research -work of the Rumanian school of.. inframicrobiology and the data published in modern medical li- teraturee Chapter I, " Botkin's disease chapter II " scleroge- :?:()us inframicrobial hepatitis " and chapter III It homologous -::.,rum hepatitis ", give a brief description of the clinical and epidemiologic aspects, the results of recent investigations on the etiologic agent and the characteristics sf the histopatho- logic lesions of each of these hepatitides. The last chapter deals with other human and animal hepa- titis viruses... _- . __.To conclude, the entire problem of inframicrobial hepati- tides, as it appears-in the present stage of our knowledge, is sunned.up_in..a table. This synopsis represents the results of the.-investigations carried out over more than 18 years by the Rumanian. school of inframicrobiology. 4a- V.BABI'S, " Selected Works.!'. (published by-an ec}.iting.boaid; acad-~St S,DNicolau,..M.Babe$, F10Horodniceanu) Bd. Acad.R.P.R0 1954, volols 64o page, LOB4,BISO Bibliographer of the works of Victor Babes . Rd.Acad. R.P.RO, 1954, 88 page The present work is a systematic classification of Victor Baber's 1300 publications, compiled according to the year of publication and subject matter in order to aid investigations in connection with the works of the Rumanian scientist,- An and chapter gives the, most important bibliographic data in the life of Victor Baber. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 ?r4.2- 6W=-ACAD0ST,oSJICOLAU, Cancer, ?d virusesi.-''r;,?.e considera? tions on the viral hypothesis of cee Ed,Acad0R,P0R0 19559 79 pago, 22 plates, 89 references, New data are reported on the pathogenesis of tumoral processes under the influence of inframicrobial agents, len- ding support to the viral etiology of cancer, The material presented is a synthesis of numerous per- sonal investigations of the author on inframicrobial morpho- logy and of the findings in this.field of investigatioA.;, pu- blished in literature up to date. In a brief introduction the author shows that the star- ting point of his investigations was the presence in sclero- genous hepatitis of pseudo giant cells similar,to those pre- viously described in many other viral diseases, In the chapter on " Cellular proliferation under the influence of inframicrobial germs " the author reviews 23 viral diseases and describesbitotic cellular proliferation brought about in the organism the activity of inframicrobial germs, In the latter part of the volume,, the phenomenon obser- ved is discussed and interpreted, The author believes that this property of inducing proliferation ("proliferigen.ous property) in the.affected tissue cells, is almost general with all viruses, since it results from their metabolic re- quirements,-viruses growing only in live cells. The younger- the calls-and-the more-active their.gultiplication, the more favourable -they are for virus growths A Russian and Frenc-. translation of the Rumanian text completes the volume, 7p? A0DEREVICIo=Prim viral meningitis. Edo Led, Bucuregti 19559 131 r-,, a9 ;rigs. IX tabs, After a brief history of primary meningitides and an attempt at drawing up- a systcmatic.list of the diseases which can be classed among these viroses, the author describes the various clinical forms. and possibilities of making a labora- tory diagnosis, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06 : CIA-RDP80T00246AO19200640001-8 glassification ,according to the etiologia agent' is proposed, The ,following chapters discuss the properties 'of vari ous virus strains .of primary meningitis, followed by a wide' description.of the morpho"pathology 'and immunity, completed' A. description is given of experimental investigations' on animals according, to, thee data "of various authors and. ' to personal reSearCho, paramount 'importance `for the'.cointrol.`of, this.viral. disease o; reveals ' the role of murne vectors, whose destruction is.,o The epidemiologi& data obtained in,.personal. research,, with data. refering to attempts made 'at `vacdination'o The book comprises -lo chapters?. 80- ACAD.STgS,oNICOLAU. Elements of .general. inftamicrobjologgo Dd0Acad0R0P0R0 ' `19956 4.. ' 2u plates ? x 77 ~.~ 5 ,Apfl5p After a "brief introduction into the 'study of inframi-. crobiology, the 'author "outlines' this science, and stresses, its paramount importance inhuman, animal and plant pathology; -. He theh syetem 'atieally analysis "oh,the'?basis.; of 'a rich docu mentary 'mate rl?al the 'genesis' of 'these=''micro"organisms and_.-.., their propert4.es `studied-`bj t" most' up=to?date_~.meens of; ....; ' - iawestigationm' "A 'description 18.. given' 'of ' ..their : itorphology `established by.- various ? optical -means ,from the simple to the...-electron mi 1roscope-, . of their 'structure and' chemical-.Land ;biological In order to illustrate more clearly .the.'particular ? be- ,- haviour'of''these germ' -in'-the human',- aniaaal-or plant organism the author describes several exper mental9 histopatha 0gfc and...morphologie techniquesg: commonly. applied :.in.inframicro- biology, asvell culture of viruses outside the organism, in. embryonated eggs, tissue cultures and tumours,. a method. ini- tinted by-the author together with professor Cmhevaditie .. .. -.In. chapter VI9 concerning the behaviour ..of inframios e . bee in the organism, acada8t?gicolau.describes the affinity Declassified in Part - Sanitized Copy Approved for Release 2011/12/06 : CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 of viruses for certain tissues, which factually implies finding of the most favourable living cvaaitions for the parasite gcrMo A description is given of the spread of viruses within the c - ganism, the mechanism of viral infection, resolution of the conflict between inframicroorganism and the organism, the in- teresting results of this conflict both for the organism and for the biology of the respective germ. Thus can be explained the spontaneous transformations suffered by the infraicrobe under natural or experimentally controlled conditionsa Studying the consequences, for the organism, of viral infection, the author describes the alterations that is place in the.cells and tissues under the action of various crobes, laying particular stress on cellular inclusions an,4 their sigpificance, accepted today by all research workerso boa AC,~D:STQSoNICOIAU? Inframicrobig infeatious,hepatitides. !d.Acad? R.P.R. 19579 73 pago a 188 references, The author studies in detail the k human viral infec- tious hepatitis s6rinogwhich he designates as different noso-~- logic entities; Botkin?s hepatitis (viral epidemic hepatitis), sclerogenous-hepatitis, nonhemagglutinating.virus hepatitis and::,}somologous serum hepatitis (serum hepatitis or Sergheev -.._._._. -.__._. ..__,__._..-. ...~ ..._ . r_ Tapeev?s disease) c, Arthropod-borne human and. animal hepatitides and animal hepatitis are likewise reviewedo-.- _. _-k On-the-basis-of-the 15 years investigations. of the . Rumacm nian-Sehool. of-..Inframicrobiology-concerning viral epidemic.-w?~ hepatitidees-briefly..related- in the work, -the author.-sustains the - plurality--of -viral :hepatitides, - iaosologie ,. entities.,that di f,fer_fr4*-the.-eti.glogie view point, and draws up a provisio-' nal systematization, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 lo. = N..QAJALe - iaborator ? diagnosis of human viral diseases,, Edo Acad?R.0P.R., 19589 585 pages, 117 figs- The first part comprises nine chapters in which are ex=- poundedflon.the basis of data in literature and the author's experience, the general methods aDDlied in the diagnosis of via ral diseases (the utility of virologic laborator..y, collecting- and and transport of infectious material, techniques for the.isolam :ion and identification of viruses in laboratory animals, QM-.. ryonated eggs and cell cultures, storage of viruse techniques;, for microscopic examination of viruses and specific h1stopa -. thologic alterations,.serologic tests, cross immunity, anaphy- lactic reaction etc.), and in the second part (7 chapters) diagnostic possibilities in the-.most important human. viral di- ceases (eruptive. viral diseases, neuroviral diseases, pulmona-. r-%T viral diseaseq,lymphoviral diseases, epidemic hepatitis, ve- nereal and ocular viral diseases). Numerous original techniques devised. in the Institute of Inframicrobiology c. the R.P.R. Academy, are described,-- and in each chapter a series of diagnosis techniques, listed. according to their importance, synopsis and partial and gene- ral conclusions are given. Next to a-rich bibliography there are also 117 drawings and original photos, which suggestively complete the text,, 110- AoDEREVICI. " Influenza various aspects. of the . problem,a . Biblioteca Medical ~ X., Bd?AcadoR.P.Re 1958,472 p.9 156figs0 This volume ia..divided into three parts: 1. Data on the micro-organism. 2. Interrelations between the host cells and the virus. 3e Data on the macro-organic&o The first part of the book gives data on the classifica- tion,_chenical composition and biologic structure of the in- fluenza virus, data on toxigenesis, spontaneous and directed variahility, as well as multiplication in syanergie and anta- gonistic conditionse In the chapters of the second part, the relations Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 446 between the macro- an,.t micro-organism are established, descri- bing the elements that stand at- uae basis of tae .reactivity of the body and the component elements of this reactivity, stres- sing its nervous histo-physiologic substrate The importance of IoP?Pavlovgs nervist conception is emphasized. In the third part9 the clinical forms are des cribed.; stress is laid on the histopathologic modifications@ determined by toxic properties of the influenza virus and the toxi-allergic character of influenzao The mechanism of the es t,ablishment of immunity is inves- tigated, mention being made of the various factors which in- tervene in inwiunogenesiso A detailed analysis of the various types of vaccines and their application is given in the chapter on anti-influenza . prophylaxis; the probleme of nonspecific prophylaxis is like- wise treated in details . _. -_ This , work is a synthesis of the anti-influenza campaign in the R.P.Ro 9 headed. by the staff of the Institute of Inframi.- crobiology of the R.oP?R? Academy, in collaboration with th$ Ministry of Health and. Social Welfare, the sanitary units in the country and especially.with the Sanitary Anti-epidemic net- work in the towns of Jassy, Bucharest, Galatzi, Brasov, Cluje Tgo Murego Timicoara and Bacauo The results obtained in small groups of persons vaccina- ted with a -vaccine prepared in the Institute of,Inframicrobio- logy demonstrated the value of mass prophylaxiso Several proposals are made at the end of the.monograph, for the organization of anti-influenza prophylaxiso 12 19GHEm2GHIU 9 S.MIHAITA 9 V 0TOMESC1f, . 10MARINESC'J,P 0ONCICIU 9 NOPOPA0 Swine lagueo Edo Acad?R0P0Ro, 19589 299 pages, 28 figs 8 tables, 516 references. This monograph is a comprehensive study of swine plague, The first chapter deals with the biology of the pathogenic agent9 stbessing its relationship with other viruses or associa- Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06 CIA-RDP80T00246AO19200640001-8 ted micrubial flora, its multiplication and sensitivity to physical, chemical and biologic agents, An important part df-. this chapter is dedicated to controlled transformation of the characters of the swine plague virus by means of its cultiva- tion in other animal species. The chapters on the clinical.,._.,.._: anatomopathologic and diagnostic study of the disease are.rich- ly illustrated by the personal observations of the authors.- The illustrations accompanying these chapters show the_-clini cal evolution of the disease, which is often polymoa; p iO and the anatomopathologic modifications resulting from the action of the virus or that of the virus associated to secondary.germs0 The study of i,:munity and specific prophylaxis is wide- ly developped and contains many details on the preparation,...., _.Zoperties and use of the biologic anti-swine plague products. The chapter on general prophylaxis discussed the epizoo- tologic viewpoint and gives helpfull indic,,.pons for the pre vention and control of this virus disease, particularly in the breeding conditions in ,uc' 13?? V0BA$ES, " Selected works " (Published by an editing board: acad.St,Sjicolau, M,.Babe-5, P16Eorodnicea:nu) Ed.Acad.R.P.R09 vol.II, 1959, 896 pages. 1. . A.GRINTESCU, C,,IACQ$.. Diseases. transmissible from animal 1_0,0 Ed, Acad.R?P.R., 1959, 489 pass,, 85 figs., 4 plates, A monographic study on specific animal diseases to, whi. c:k man is also receptive, diseases currently named zoonoses. Be- sides several viral and bacterial diseases listed under this__ . designation, all animal diseases of viral and bacterial.origin' as well as mycoses acid parasitoses that tkireaten human communi- ties, are dealt with..... The subject natter :i.s divided according to the causal_..._... agent, and in the introduction general considerations are made.. on the role of natural foci, on the common sources of infection and infestation, on epizootologic and epidemiologic characte- Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 risties, as well as on the principles that;; stand at the basis of the prophylaxis of these diseaseso 15.- G.MARINESCU. Acute infectious lymphocytosis and infecti- ous mononucleosiso Ed0Med0 196o, 309 pag., 4o fig. New data are brought concerning these two diseases _. little known as yet in our country. They have been described. together in i.ew of the marked tropism of their causal agents, probably viruses, for the reticulo-endothelial and lymphatic systems throughout the whole organism. This is mentioned in.. a brief introduction,after which each of these affections is dealt with in detail0 Each chapter is of a corresponding didactic and scien- tific length, stressing in acute infectious lymphocytosis, th@etiopathogeny which is still under discussion and hemato- 1oRic alterations, that play a principal r9le in establishing the diagnosis. The chapter on infectious mononucleosis deals 9 .> .a ..,Y .1 especially with the maltipld. 'linical forms and the possibi- lities of an early diagnosis, The book is illustrated by many figures and a rich bi- bliography (2000 references), comprising the original works of the authors the contribution of Rumanian investigators and specialty pubilc ations in the last lo to 15 years. 160- V0TOMESQU,.I0GIiEORGHIU,. I0ISOPE$CU0 Foot,and mouth disea se"0 Ed.ACad0 R0P0R0, 196o, 409, pagos 85"fig0 The book presents the newest data in world literature,.- the contribution of Rumanian investigators and of the authors to the main problems that stand at the basis of our knowledge and the control of this terrible diseased The following chapters come after the introduction and. the history: Study.of the etiologic agent, Receptivity.-.-Exile, rimental infections Pathogenesis, Epizootology, Clinical stu= dy, Anatomo-pathologic study, Diagnosis, Prognosis, Immunity_ and Prophylaxis. Particular attention is paid to the physical and chemical properties of the virus, citing data from world literature and especially the works of the Rumanian scien. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 - Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 tists Levaditi and Nicolauo Study of the plurality and variability of the foot and mouth disease .virus is founded upon the initial works of--- ` Vall6e, Carr4, Waldmann and Trautwein and the new publications of the Soviet authors Kindiakov? 13aiadinove etco Description of virus type determinations begin with the method introduced by the Rumanian investigator Prof 0 of o sI CA in 1929, complement -fixation and the modern methods s neutrali,A?ti nn In tissue cultureso authors discuss in detail In specific prophylaxis* according to various me- thods, the preparation of sera and vaccineCOntributior to the prepa~ and likewise scow their own ,ation of the vaccine done for the first time in our co ntry ,:nd which led to eradication of the 1959-196o . pi t o VOBABESO 19 Selected work s , B ,, voloIll (under the.case of, an editorial boat~d? 0 AcadhS'oNicalaug t,BabespC,Uari.nes- cu) EdoAcad?RoPoRo "196l9 298 pago 18m- ACADoS ACAD0AL' RADULESCU$ N'oe',,C0IISTA1jThlS- ''dSo~ICO~A~~ CU, R,CA3AT,s QxoMAgIWSCU.Polior i?s Ed0AcadoRoPoRo 19619 ??1 page, 40o figso -? PO~,Tp~: LITIS is written at a high scientific levels under exceile.nt editorial condition,sq and presents the latest data concerning one themost important and actual problems-in di- m ,decineo The twelve chapte)ra of the book deal vitastct sd.i to date with all the p sease completely and are up this affections which has preoccupied invest:i_f:ators for al- most two centurieso Chapter I gives general indications on the etiolocy, Chapter II shows the. immunity and prophylaxis of the disease epid.em.i.ologic aspect.., pointing to the main epideniiol0gic fac- tors, the contagiousn:5s of the disease, its mode tion and sources of infections and particularly its evolution of the causal in our country. Chapter III is a detailed study agent and deals with its morphology and chemical constitution as well as with the reaction of the vh~uvzrus various especiall.y in the and physical ag-ents n cultivation of tissue culture,study of different virus st:ra.ins~ ei Chapter IV reviews numerous Investigations regarding Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 - _experimental reproduction of the disease in more;e mur,~ nes and other animal species. . apter V relates the invest gatioms linked to the physiopathology of poliomyelitis.' and ckapter' is concerned with the nonspecific factors that influence the clinical evolution of the disease. In chapter. VII there is a detailed study of the lesions produced by po- lioviruses in different organso Chapter VIII presents the most up to date investiga- tions and the newest methods for isolation of the virus, pre- paration of type immune sera, virus i dentificationo In Chap, ter IX the authors deal with problems of antipolio immunitye both active and passives paraimmunity and interference in. poliomyelitis, data of exceptional interest from the practi- cal standpo i.nt o Chapter Z comprises specific prophylaxis by means of antipoliomyelitis vaccinations Chapter XI describes in detail the symptomatology of the disease in all its phases of evolu- tion, the clinical formsg.respiratory imbalance syndrome: in. the course of the, disease, complications' positive and diffe- rential. diagnosis, prognosis and methods of treatmento ft-_ nallye Chapter XII is an ample study of the period of seque- laeo POLIOuYNLITIS is also a review of specialty scientific. literature from abroad and that of the Rumanian investigators and olini:,i,anso The exceptional contribution of the Soviet School-in-solving the different aspects of poliomyelitis--and particularly. the sucoes obtained by Soviet scientists with the antipolio vaccine are also described, POIRIMELITIS is useful both for specialists, in this problem and for doctors specialized in wt;Ler fieldso Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 !4,52 BA x7. G o = -7 75-9 63.9 95 1. 9 14488 o X52.9. 16.6. 225-233. 6949 X9897; 4360 476 9 477 9 4799 7139 BARBU Co- 1S0? 4949 7219 BARBULHESCU.GE.o= 3459 BENEDICT. I312 . BERCAN A 5299 570, 5739 63o BERCOVICI 00- 163. BERLOGEA 10= o69.613 0.642 6848 68,E 686 BIBERI-MOROI .So0 1599 1629 175 BIICU NO. 161 BIRZU I 93. BIRCA Ao? 1969 1980 199, 2o3, BIRCH No- 1329 149 142 9 1539 BLI DU Po-. BOCIRNEQ. C o -a BOERU V o- BOIU Ao'. BOTAN A0 ? BOT.EA Go= BOTEZ Mom BOTEZ Qob BRAN LO 2769 27.79 2819 2829 3.030 3o4s 3?59 3o7 1429 2o4. .30. 1459 446, 397 399 661. 245. 1589 1629 1630 1736 178,_ 2889 2899 29o, 297929893009 419 47, .128, . 0 1369 1370 .1389 -- 139,0143.0. v. 1549 156t, 1579 1659 1699 2360 3014 44'4'9 '59 4480 4589 4679 4699 4739 474, 4759 492 1129 124 1.91 548. BRAUNER R0='.. BRONI'i' Alm- 348 9 379, 3989 4269 4639 700. BRUCKBER. 8 o- 121 BIUCUR No- .. 608 6o8. 3499 350, 353, 3569 3589 3629 3639 .383780 3809 3819 3829 3839 385 0 386g. . 389 ,, 396.9 397 s 4oo, 4log 411 a 413 0 414S 419.94269 424,425 9 42.79 4309 4339 4409 446,449,457,459.,4619... 46494769 9 4799 4909 . 9' 6820 68396959 BURDUCEA O o- 35 9 2o2 9 479. 9 715 9 717 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 . ALBOIU 5299j 126, 128, 129,146, 71 g 39o, .196, .1984 19 .,2o1, .5 9 257,261,267,328? 0_.g4 202920.3, , 2410 38484, 492,6 929.4 ADERCA I.- . 28 9 32 .a ? 9 34o . 369, 3704790.9 9291160 117, AYI" a .TIC_ I C U A ABRAHAM Ao c 653 ALBU C`o~ 9, 10501139 515,516,682 ALBU ToT. 523, 526, 529, 53,01x,331, 533, 5689 5709571 A7.SDEA M = 449, . AI,ERANDRESC1' .Ram .614, 615 ANAGNOSTE Bo=. 62,,, 67, 68, 699 70-1)-72,)7.39,7-69 78, . 8o, .81,83 ? 84 .859 194 2919,2949296o 3649 3659.366,367, 368, 3699 370, 387, 403, .455o .6129621,625,626, . 628, 629, 6309 631,632, 6339 637, 638, 639,64o, 64.1 m ? . , * __ .. , --. . , ~, ;. ANDREESCU M,- 40, 147t 1509 168, 17191799295, 470, 0297138 ,717,. ANGELESCU Ho- 594 ANGELESCU.Mo= 299 ARMASU V,m 219, 437 ATHANASIU p 210-1129 1 ,. - I. , 168, , .17S,2o2,2o4,222, 2269 25 59 02619.26 .266.,.2809 292, :. 375, 386,. 3 ,. 4079. 418,42o, 429, 4416 4429 BABA Co- o= 4.53, 4549. .466, 7?'.., .48o, .497, 4.989Z?oj; 4,539 5 ?3 550 .626,, 630, 6319 632, 633, 634,653, 7119 7129 714 B 136, 17 , 138, 139,1409147, 1519 154,156,1659 1.69, 170,2029.232 9 .702. . BABES Mom ..48.9-250t , 6899 EL69 BACALOGLU. Do-6141.0 BALAIAN M0Soa223, BALM A0= 684, 685 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part- Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 BUSILk V om 60, 6.._.,,14, 6159 624 BUTTE V o?' 684 g CAJAL No- CIORCITINA U q~ CIOCITLTEU V ?m CIUCUty,CCU - A?? 24? 2 , 3 ?37 12, 17, 59o 6k,86 g 87, 88 ....~' , 104 105, ? 1088 .107, ? 108,9 lo" 1??'1?x29 _....,,' 1249 1269127+ e --... 122 9 1c) 111 ? 113, 1149 115, 1]L8, 1+ 9 1140, 14.5014 L3 8 1:19 128, 12 1 9. E 136, + 1.54 156,1.59,160,1 175,17, , ? 184 186-19 ?. 9.~ .l.Z6 a ?'. 1809 _' ?- 177). 179 s_...-.-- 2249 225,j ?. 201, 229 20.. 212 214 21 + 234 2390 240 244 233 ,_ 22 ~ 22?~ 22 0 910- g2 0 2 28o a 28 6 1 298, 247, ?16,," 8, 326 3" , .3479 3521 392{ 3? 303'. 330 5 5 e .~ 88 899 .644, 6450-656 393,!' .44.39 5o6, 5349 ON 672 674t.6759 6779 678, 6799 661, 6689670v:-6719 68o, 69019 0ALWOV10'( S 1919- ~.. 212, 236, 422, 4719 CAPRARU RO. 92 9 93' 1o4?e 110, 111, 135t CARANIAN vqm 574. CABA11aAN - zo- 593, CARP No- .- 505t 571 CARUNTU Fm-743. 114 124,_? CEPLEAI+7~J m 2 , 35 9.86 86, 104+105, 109, 1.13, 149 201, 202, 239,2599 298, 3o7, 3159 326, 135, 9 . 3529 4229 471, 5479 6o4, 715+ CHEPTEA Ao' ' 269, 270, 2749 275 CTrSTi TI1 HLTAI.o? 3o1, 3369 621, 637 OBA"-850't) VIO- 202, 220 , 4899 493 9 CIURBSU V9? CIRNARU 8w? COPPLER 11 0?' - COJOCARU Vo' COIPANET V ?~ 590. 5919 592- 594 185, 1.88; 189, 37:e 4149 4409 14 p X2.166 3 , 370 168 366. 44+ 498 9 714 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 CODREA Ao? 345 .. . CONSTANTIN.ESC 3 A o 49 4' . U CONSTANTIN:r,,~.`; 577 660 CONST,ANTINFSCU ~; @ c 329 162f, 173, 178, 2~1.. _. ? 24.8 j g4 ,251, .2529.2536254, 257 26 CONSTANTINESCUo?. 4o4. CONSTANTINESCU Nom 12 ]&1- 265i 2 . o20.30;r 304.0 3030 3929 651.6. 2, 281 ' 28 - 8- i2 .2~ 8 , 28 x_297, COPEEI.,OVICI Yo= 24 26T 3 CORTEZ Rom COSTACHEL Qom COTARCEA Io- t ANDACHE Do= COOS3' COVACI oas CRETESCU A0=,. CUC IiT@EANU J ?? CI] SEA . V CURE Co- CURTEANU Go. g g 5, 949 110, 111 114, 126,135, 1 .,t 149 2020 212, 214, 215. 217, 219,220, 235, 242, 32 ~' 419 422 _ , 437j,4380 46-20 4669 482 48 9s 4930 496, p ?15p 331 .... . Is ZI-1 82 4.64 , 168, X809 494, Soo 372 695 4o2 627 299-o5o1., . 5o2., . 5446 5559 5639 564P 565,566, 567 3459 354, 4o5, 56o, 561,562, DAMAN. Mo~ 22o. DAN Ho. 655 D A F T p . 577 DANESCUopOPESCU Go see POPESCU_DANFSCU Ge _. DANIELESCU Goy 7a .4 -7 117,. 3259 128, .150, 176.9.._198' 1999 2o2, 2o3, 2610 26709 483 DARINGA Ao o a 590 D.AVI ESCU 2o DICI Ao1= 5 _ . 1? 3. o ? 6.~.~.g . 30.g ,fie-- 66, -~?? 3_?~a. 100 311, 3120 9 9 d9 3169 34 934. Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 3449 3 4, 2'8_.9 33~~ -3 1 ?, ~ ? ? 3 , 6o i .361P, M2j 36,L . ; 3?8 3 638? o ; . 2-14. 82.t 3 k 3?, 395; 396o 32 Zo; 39 . 406, 408, 411, 413, 4239 424 k 42?,f 428,.~.'~?, .?9 .9.39461 463,. 464,4 b4779.478 . ,e4-_-488 194919 5949 6660 6, - 673. 6816 . 6829683, 7, 6889 05 9 00 7 , DIAOONU J'o- 495 . DIACONU So- DINCU.LSCU P ?- DINU I 2oo 583. 1459 1579 2oo. DINU R.? 102, 112i 2d , 124. 131, 1829 2269 23o9 26 0 o . 264, 314, 3159 3 0 5o9, 51o.511.. DINULESCU Go- DOBRESCU G$o-' DOHOTARU V0.. 512. 508. 500- 595* 513, 5149, 311r. 509, 5109 5119 576, 577,: 578.__ 5169 , 652 517 DRAGANESCU No- 18,.21,, 25, ,184, 185, 1889 189, 191-9211' 21319 j?1', 3309 ,13-2-. 2839 2840 32o9 3219 3319 122-9 , 350, 293, 322, 39 353, 294, 32.39 334, 3559 29.5+ 296t3o1,. 324 3 3290 335, 3369.337, 358, 361,33 3649 365 o. 3669 3699 3709 376,38o 0.388.0 3 9 42?.,..434, ? 4359 439, .455., .456, .473, 499, 9 .,,6sL 6ol .6o4, 6o3,6o6, 6o7, 6o8, 610.635, 6369 7o7. : RAGANESCU SP o? 1 1. 98-3-9 3L 219 2 3 9 . 3, e 4 652 DRAGATOIU V - 155. DRAGOMIR Co- 249, 251, 2529 253, 2549 2659 59 e593 DRAGOMIRESOU Do- 1o2 . DUCA E9- 257, 265, 2699 5909 5939 651 DUCA No- 257, 2629 2659 2699 593s 651 DUCA Po- DUMA Do?. 627 9 638, 639 DUMAS NO- om 6o3 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 DUMITRESCU Aw- 538 ?DUMITRESCU 1 565. DUMITRESCU STe- 2o,.39,.40,128, 129, 2o8, 2119,2139 221,409, 446, 46,7, 4680 475, 4.87, 579 622. DUMITRIU.Eac 501, 5o4., 56o, 5619 562, 563, -5 566,567, DUMITRIUUCAROL Eo- 299, 564 DUVAN X.e- 134 3D.U`;S- 505 da: 372 ETEFTERE8CU Ao=-^ 306, 5o4. ENACHE Aom ENACH .Td- 584.56.9 7o6, ENE Amy . . 453 ERACLIDE M0= ..594 EUSTAFIEVICI C0?577 5289 532, 577, 584, 585, 586 FACON Eo~.? FITARAU Ao~- FLECHNER Tom FLORIAN $o? FOARTA NQ FRADIS Aom. FRENKEL Mo- FREUD S~ 609 411 689 Lol 362. 137, 154 163 FRIDMAN 10? 1859 1889 189, 2o8o 209, FUHRER-ANAGNOSTE Be- see ANAGNOSTE Be G. 6469 647, 648 GAME-GHIA Ro- 357 GARIBALDI Ao- 155, 701 x. jESCU To- 435 GHEORGHIU I,,- 519, 520, 521, 522, 523, 525, 526,. 8,x 6,x,532, 9 568, 571, 577 -- ~. GHEORGHIU Me- 1329-1410 142, " ^ - GHERASIMESCU 191 GHETU..Vom__.- 194 GHITEA Do- 345 GIROUD P.- 6 5gZ a 602.E 6o3_ Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 457 196, 1.980 199, 2019 2o2, 203, 238. 2-219 2439 245, 246 9 328, ? 484 5779,578- 659.719 82. 626, 6319 632 399? GRUTA M.- 91, 2o2, 217A L-1 2j, _? 3o9, 31o, 311, 312-o., 311, 3159 316, 343. 349, 356. 3629 363, .418, x?199 o 2,438, 462, 482, 5069 543, 681, 682,6839 GIRD E.- 294, 296 GROZA M 22o HUNG TAO.- 2440 599, 611, 612, 616, 617, 6189 621, 622, .loo . GRUMAZk;SCU no-1320 153. 158, 162 GUNA 80- 389 HARAGEA $.- 474 HONDOR CO-.- 9... 412, 712 HORNETZ THo?? 182 HoRTOFAN D,- 101. HURDUC G.- 399 229 . . I . IAOOB Co- .2600 266.0 272, 5159 5169 IAWMITEANU M0-171, 179, .. IADICON SCU Y?- Z89-329-3.39 349,3-.64--M& 479 559 1469 17691906 IFTIMOVICI Ro- ILIE B 0- . ILIESCU.C0- IOAN E IONASESCU.Go- IONESCU Do- IONESCU MI-. IOIgESCU N-10 IONESCU,Y'" ISAIA G'oem ISBASESCU AQ- ISBASESCU C o- ISOPESCU 10- 343, 682 ;S89'- 370 39 40 9.51,_284, . 293 1 .2940.. 295, 29r o., 3o 1 J3o9 , X46, 4679 4689 473 4.95-, 4879 579, 6o4,6o6, 622 2414 476, 488, 489 155, 7o1 516, 51.7 3o6, 524 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 ISRAEL Mo?. 350 ISTRATI Me- 485. IVAN I o? . . X22 0 :3? IVAPOL Mo= 2oo ;:SZAA So- 696 JEVANDROVA 1101 o- 223 KAHANE G 681 KAH.A NE R . 91 EAUF : So- 161 SEIDE v,L . 44o5 KOS 10- ..662 REINDI R AoC,227. KRESTESL Ro- 6290 63o L TACK Hoa .4000 5949 6o8 IAAZARESQU 0x0'181 LECCA Go= 219. . .. LEIBOVICI *~o-1320 1410 1539 158 LEONESCU L = 1360 1379.139 912 LISSIFVIQI=OPRESC!U EL,= see Oprescu.Elena LITMAN AD- 400. LITM` N 8 138,. 139.0. 148 0 1519 152 9 156, 16,, 169 s I,IUUCHUNG=P&I0-14? 218, IAZINSCKI. 5o4 LUPASCU Low 578 U WAIITEI E .632 RICATIDE ELo- . IA1ICATID. T 0=453 IOLIU.No? 37. TTA le- 1329 1589 MAIRG, a oa . . 493 MARCY;LESCU -1, - 50. NARUARE. 10d 634 VIUMS V 4999 722 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 - Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 718 MARGARI`[' z0CQ' . MARINESCU QrH. = 35 0 121 .1249-1349.1 9.181,9-18 0 188, 1.89 9 e 19 a . got 2029 2o48 206 9. D2 4 2184 221, 2229..2?-39:P-319244- , 3159 33 34 69..3 ,..3570 22O ii; 88 4 41 9 0 0 451, 452.0.481.,489.s 0 4960 5169 517, 5889 591fl 592 596 597k 6o2, 6o3, 611,0 613, 6160 6179 .635, . 0 .64:39 65o, 6529 6 9 6550 X60 .659,,.66o., 661j 662* 66L 694- 6979 694-2019 715 9 713,2 Z& :b. 9 i 1 ' U SCU 1 , 7 . 5259 526 MARINESCU. SP.w? '5'919:-.592 . ova MARINOV L o??, M 519 9 52?0 .. 52~K9. 52.2 MARIA M 627 9 638 9 q _ loo l04 UATEESCU So- 12 1- , 359 8 93..,0 959 980 999 .0. , k 1o6 9 log s 110 , 1110 114, 123 9 135 9 182 0 2121 214, 2150 23o, 2414, 2560 259. 2689 421, 67o, 6730 692.9 6939 715 320E Do- . 4o5 ZTERCVICI Ao-. 200. ' SROBEOU limo 115 0 145, i ICHEL I. 374, 72o CLOSI.E.= 345. CU I , = ? 142* 1739 288 2` RAIL AL.= .. 132, 1789 ..-U AILEANU .1, 128- 531.0 532 0 2INCULESCU P.:ERSAN o'' . 9 53 . 5359 5 ~0 ''539fl 'S z0 0569 0 !05.9 4jLq, 66?- 453 MTRICA Mo? 115, 145 IITROIU 00? 134, 148'152, 1 619 1659 166, a 1.8o,2229 436; 4379 4389 4669 4949 5099 5100 51295139 514 5159 5169 517 0 652o 9 721 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 29o, 3030 3o4, 3o7 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 466 ~. - . MIRZA?EMINFT Lv- 1.09-119-149 22 ,. 299 54-f56 .263,. _,, ,27,11. MORARU A= MIRSAN M?m MONTAG lo-.. HUNTRANU.Gecm MUNTIU $??' MUSAT B0= MUSETEANU C0 ? MOLDOVANU Go- NACHTIGAIT. CC= NASTAO E0= AUMDc. ,? NEDELCIU Do- NEGREANU W,m NEGREANU Y,? NERANTIU F.- NICA Am= NICOARA I?P NICOLAESCU 19 NICOLAESC1J _ N o?e NICOLAU CLom NICOLAU ST 0cm 2 29 2789 292, 12293489 3719 Me 3,,86,,441 7o6. 3o5, 307 543 6o9 142 412 439. ,591 592 577, 578 N 32 9 339 34? 6o 61 s J. 64+ 6 - 68j ?,2# Toe s Z2 L6 75% 9-77 ,2. 22 8??q .81, 82., 83 8? :' ; 194, 26o, 266, .272, 403, 4399 ._455.,.. 516, 6o9'612* 615, 621, 637, 6390 64o ,698, 4899 495 5459 546 161 585 329 531 372 95, 106 655. . ? -188.284, 293, 294, 296 . -Lu 19.2,8, 2.'. ,-.222 .c.?2., 56 v ?2, - r 0- _. -G~ 9 1'2s 10B, '9...:d.9' Z, #-i u- 102, A 113 114e 124 1 16o# 162e, 22 7 __1749, 175,1 177 a 181 ~182 -_ 19,5~201i L2 23 9 5o 19 ZL2 0 . . ? 1 3 0 Z .56.g 3 9 2 6 1 . t 9 273 284? 2911, 22,9, 9 294) i? 6 a 92, 9 3 .,_. # 30 116.1 44o6. , 2 s 2.. * .506.L, 2081 1vg 5l ?e. X13-L 514+ 6 _ 626,E 63I& 6...322. M" 634 644i 65?.9 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 2!; 66 bog 672-9 673p 674v 6779 A L89 M9 6869 689, ?3o,, 6?21, 6229 694# ,7o8, 71?' N COLAU So.- 200 NICOLAU V O-.. 594 NICt)1SCU V o- 161 PASCU Lo.= PALAUE Com 168, 66o PALADE E o~ 593 PANAITESCU Go@ 178. PANCU Vo= ,4549 496 PANDELESCU I 594. 0 OANA C 162* 163, 178,399 OLTEANU I, 227. ONCIOIU PO- 525.9 .526 9 529 . OFRESCU Eo= 259,98-9_1009 104, .109, 110, 111, 114,__135, 18.3 9 1879 194? 196, 1.979 199, 203, 228 0229 9 2340 259, 315, 3349 337, 403, 455, 605,607, 6o9, 612, 614, 621, 637, 639, 64o, 6619693 OVANESCU AL 132.. 173 P . PACURAR!, E o= 214.+ 421 * 487, 479 PASCU M o- PASCU T PAUL Fod. PAUN FI,mc PAULA L0- PEIULESCU,Y PESCARU Ao=,. PETRESCU AL@= X8505,,5245254 528, 5329 52,555,568,677, 58o,.58:, 585L 86 699 594 499 4,349 453 643 2os2 437 21, . 56,. 292, 348 9,..349,, 350-.9 3519 3539. 356.9..-. 35.89 363, 3759 378, 380, 3819 3820-383.9,.385,, 3869 39o, 396, 397 9 398* 7, 40'6 ,. 411.8412 9 4130 4148 418* 42o.. w p) 4214 4258 426 42794299 43,0, 431, 432, 433, 4400. 441, 44.2, 449,4.53, 454, 46o, 461, 480, 497,_498, 5oo, 543,6839 6959 loo, 711, 712, Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 PETRESCU Ago- 191, 283. 323, 3310 434 PETRESCU P.?== 375.. . PETRESCU -COMO VG= 434 PETROVICI ..L0= 2o. ? PETRUSCA J .., 3539 3890 397 PHAM SO1 G o= 471 PICOS Ao= .385 - PIRONCOF ALo= 3530 389 PODHORSKI.E 115 POIL? ?M.10= 32 POPA Be- 5489 585 POPA Iod 3979 4110 412. 712. POPA M0= 1610 1679 18o, 5248 5259 5260 5289 5299 5300 5320 5339 .5440 5689 .5699 .571.9 5766 5779 578,,. 5790 390 4365 4560 472., 4949 7139 721 POPESCU Attu- .5190 581,p 583 , POPESCU GH0= 4o6 POPESCU GDMESCU Go- 1040 1?99 11o9 1110 1160 1359 14oo. 17op 1760 1870 2020 230.0 a 2739 2860 287, 3o2, 3o8, 229 39., 0 9 .1o POPESCU I o= 217 __ POPESCU PRETA I 168 POPESCU 5810 583 POPPER M0= 161 PORTOCALA R0=.r?x~5~~: Zpo 23b 3e0 9, 41 s s 4.0.0 51~r r0_, 87n 8805r.1020.101 14 .~1 j ?;,: 224;.s .349 5~2 D 2 2472, 267- 0 279o .2 ~8o 640 ~~84 40 46-9 i 444 _q. 446 !tVA 4481 467k -k oD 122 ojg 6o4s 6o6b' 6444645) 6660 6 219, 6720 6740 6759 6779 6789 679,680, 9 7080. 2 .--,- .... , PREDESCU I9= PRE]DESCU L0= 2000 2o6m 400 .415% 41.60 496. 204 4649 4860 4880 489$ 4900 4,01 PREDOVICIU F0= 167 PRUSKAUER=APOSTOL Be- 435 PUIU Bo= 2519 252 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 +r = ROMAN Atom 6380 639 RO-AN Poe= 185 ROSCA 1o=' 4.82: RUSU Fo= 1730 399 RADESCU Re- ,.626, 631, 632 RADULESCU ALo?' 206 RADUL SCU Co- 515 RADULESCU i 5o8 0 5o9, 516, 517 RADULESCU Mo? 374- . RADULESCTJ. Pb? 2o6. 491. . RADVAN AID= 13.1, 1430 145 SABARES8 ?Io? -y : , 211 2139 g 43'.50,, 56, 68, 739 129, 208 SAMM 10- 669, 370..0 374o 4-22109 438 i s327-,3 s 2?2, NI -2 9 474 448 4.58 0 46 7, 469 474. 5 0 4440 4459 4470 - 4920 6/x60 64 648, 649 ? 703, 7o6, 7169 SANDU Io? 5940 647, 648. . -. 0 SANLULESCU Tom 2o8, 4o2, 404, 437 0 646 194-9 221, 315 * 321 o.? o . 61,-70,769 850 90 ? SARATEAI~U Do 34.3, 3450 348, 3499 350 s 353 3 35 8 369, 37 36303 fl3.?5366036 _7,369 88' 403 418 4396 ,38o, .382 , 383 ? ~ ~-- s * 4-57o 57.50 588, ? 5940 6oo, 601t.604 456'.+ ? ? 61? Cil 6128613 .614, ?0 60 , 608, 609 0 .a--~- + .---0 621 622; 624; 626, . ,?119,6169,617t 63o a 631, 632, 633; 636t ,o. 629-064o 6410 6420 6641 665 620 637x6380 ~3~0 --- 0 6940 7ao0?70 6810 682@ 683 0 693 8 . + 89 0 3970-.--411 X12. 4.27., 712, SATDtARI C?? 3530 3 SCHOENFELU S.o? 3o80.613. 642. CAo~ .95 0 1o6. 182'. 509 0 510# 5110 512 9 513 9 SEPEANU FIRi 5140 650, 652+ 60 SERBANESCU . C 54o-', 5.5? 0 - SFE'RDIAN 00= 179 04'94 s 721 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 _ _ SIRNU No- -. 521 SOFLETEA So- 331- SOR0000 Gol 17OD 221, 5720 573s 6280 629o 63o9 6319 5740f7 6329 6330 620 6380 6250 6390 6279 64o0 SORU Eon. 641 910 jl~q 5 667 SPIRU Io= . 122 -. SPRINCENATU DOOSA E,o=. 663 ST..NOU Aoo ST.GCA Mod 3530 3890 411 5310 533.. .. STARK iOT. 2000 4o20 404 STAVRI No?. 27,7 S?.ERESCU Lo= 364 STERE CU.Po- 181. STIRBU Aoo 27o, 2740 275 STOIAN 10T 2749 STOICAN Vo 527. STRATI To- 3o8, 613, 642. S?ROE A0= , -.1810 219 691 STOR-P,SCU AoMe- 719 ST?= E? SCU C o? 402 STRCESCU Po-. (see AhanasiuuStroescu STRULOVICI Yo- ( see Copelovici Yo) STRULOVIQI D 500. . , . SUUACI Io. SURDAN Coo Po 0' 524 0 0 56 0. 537 0 5.38 m 5399, 0 ... 5.10 5,42 5 6 5~ 5,, 548 0 542# 551, 5530 fl 5566 5576 e 5,59? 582-- ?22 0 5ofl 5020 w o 535, 536, .%>8 X00 5390 541,v .5 a 5530 s 561i 562, ,500 o .20o 5?6fl 576 571,0 0 52 ~ q 5750 5770 o X90 5840 62A? 625:8,.62606270 6289.6290 a 631 632 0 633 s. 6 8 0 639 0 64o, 641 D 672 TAGA N0= 2o40 535, 5360 5370 5380 a 54,0 e 5410 5420 551 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 TAINDEL Co- 270, 274, 275, 404 TAINDEL Icc . TARCHILA D.? $o, ! 1+i 4o.5, 414, TEODOROVI01 GR.? 265, 277, 364 TIBREA Sea= 5059 532. TICAN V... , 58o* 581, TTGAERU N..- 585 TIMERT AAN A. -353. TIRNOVEAI.Gem 703, 716 TIUCRE.. A.- 64-3. TOMA A oe , TOMESCU V.? TUDOR I TUDOR V.~. TULPAN "'.? 1URCU Io- UAN TM LI$No? URSACHE Rom 582 440, 449, 5o3, , 524, 525, 0.69 557, 589, 576, 5789- 582,_582 399 138,.-139, Me 6, 169 98, 95, 106, 185, 188, 189 U 487, 6,13. 548, 5199, 5519 5539 503. 541?, 548, 549, 553, 554, 556 557. 558. 559, 578 V VACS Le-.. 142,..' VAINER Ho- 91, 669 VANCOV Za= 161 VASILESCU I om- 6o9... , VASILESCU,Pro- 3,W,4, 5, VASILIU Gm-~ 347: 666, 668 VASILIU N9- 509, 515, 516, 517 VASOIU FL. 288, 3o3. VATASESCIJ. Ao?' 134, 148, 152, 1669, 686 VELOIU V.= . 634. . VELICICOV V 235, 242 VILCU?$OJINCA 1.-.453, 498 VIRF L,= 372 VLAD I94 4o5. . VITA A.o- 59o,'593, 651 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 to 312, 316. '344x 3749 415-9 ?' ' CICULE. SC~" w oa 2 309 a .3 r 41 , 4510 01?+ --+ `VOICULESCU. R.o? 2o "VO. NESCU I o? 322 'JU1-,jESCU s.-. 323. VOROSII A oKo= 223 'aCE .R o= 5019 5o2, 567 V EI TRAUB. L 493. t EC3 + . Rol= 647 0 648 5?os ZA IR C o- ZAVATE O o~ W 5o4, 555, 560 , 5619 562, 563 0 52 5??s 532 z 95,.106 . 1.3.2 141, E 158, 162 0 2880 297, 2980 3ok, 305, ? 163, 173, 178 307 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246AO19200640001-8 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8 THE WORKS OF THE INSTITUTE OF JNFRAMIGROBIOLOGY OF THE R.P.R. ACADEMY (ABSTRACTS) 1949-1961 Declassified in Part - Sanitized Copy Approved for Release 2011/12/06: CIA-RDP80T00246A019200640001-8