THE ACTION OF QUATERNARY AMMONIUM COMPOUNDS ON MICROBES
Document Type:
Collection:
Document Number (FOIA) /ESDN (CREST):
CIA-RDP82-00039R000100100065-9
Release Decision:
RIPPUB
Original Classification:
C
Document Page Count:
18
Document Creation Date:
January 4, 2017
Sequence Number:
65
Case Number:
Publication Date:
February 9, 1951
Content Type:
REPORT
File:
Attachment | Size |
---|---|
CIA-RDP82-00039R000100100065-9.pdf | 4.5 MB |
Body:
Declassified in Part -Sanitized Copy Approved for Release 2012/05/25 :CIA-RDP82-000398000100100065-9
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0~' gUAT~RNARY ,~SUNIUI~ CONN~OUNO?5 ON ~ICR0~9 by
Tltlvs T~iE ACTTON
~ ~d V~ p, Ura~?n~kaya (UDR) , ~i
N. Mv~el
11-19,
1 solo a Vol XVIIS~ No l Jar~~vb 1~1~9,
SOUrce~ ~icrob onth ~riodic~ 50X1 HUM
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Declassified in Part -Sanitized Copy Approved for Release 2012/05/25 :CIA-RDP82-000398000100100065-9
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rodeos wore di~fiault to diecQrn a~t this
two minutoo~ ThQ details o~ the P
wa also used r~nnaentrationQ Af 1~~O~OQA,...1s100,000~ and
opoed~ ~~ez�~nj a
� the rooeoo oaourred more slowly ~d we worn able to follow
tl~eee Q~oe _ A
in
Tho firot thing whioh was notio�d
atop by step tho basin aellulcr ohangee~
soA o was the sudden intsnoifioation of vaouoli~ation
with an ordinaryr micro p
` 1
influence Af oetyl-aoph~rol~ The protoplasm became
Af thQ Doll under the
hat it seamed to haYO frothed the wall of the
full of large alvoolos so t
ed and began to rQfract light more stron~ly~ I
noted elatini~ ~ 1
0@11 cant ~ g ~
{ a e of contraction of th� protoplast a~~d plasma sc that the
Later ensues a et 6
ear The contraction of the protoplast `
latter is ~illad with small avcol
tit deemed that the droplets of lipoids and nuclear �tr~~ctures
increased so the
cad small grains The contraction of t~~e proto-
disappeared and were reply by
at se stated from the cellular membranes giving a
pleat was so great th p i
@ver this is
(Fi~?~re 1 b } ~ dew
.1 picture Bugg�sting acute plasmolysia~
Bible state Staining of the living cell w~ h Janus Qreen
already an irrever
ar�r~t further details of the cellular changes
pad makes app
and 1'ieutral
riosome structure ie affected 1n the early stages of the
First the chond
r~sis d@velop in the
r reaction vacuoles and lipoida (fat phan� }
cellula
hen these organaids expand, assume bulb or spher~.cal shapes
chondriosomes ~ ~
ter art of them loses tl~e ability t� be stained by Janus Qreen
and the grey p
ed which are then retained only by the peripheral structures
ar~d Net~aral R ~
u disNerse in small granules, and dissolve
Later the chondriosomes break p,
er bQ ins to increase its adsorption of the dye
in the protoplasm The latt g
colored in blue by the Janus Green and in+ red by the
and bQCOmea diffusely
bat the rotoplasm and its organoids were under-
. Neutral Red ~ At the time t p
es the contents of the vacuolar structure for
x: going such considerable Chang ~ ,
The volutin which Forme a coacervate with ,
a long time remained unchanged.
1 ickl dissolves during various affections of
. the Neutral. Red and usual y c~u y
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r ~ " ` � ~k " ` Declassified in Part -Sanitized Copy Approved for Release 2012J05/25 :CIA-RDP82-000398000100100065-9
Declassified in Part -Sanitized Copy Approved for Release 2012/05/25 :CIA-RDP82-000398000100100065-9
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t ~
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s
ee remains for oomotime in thQ farm of red ~,renuleo and
the 007,1 in this oa
only /star deaolorieee
len ~~n0r 0hen~ee w8r@ et~~~~v~d ~~F ii's~~~ L~YA19~?Ar~tit~L%~A~L'p~~� QA~+�~I.L~IaY~iJ~
under blue rove ~ ~'or this study the cello w+~ehod from th� auopeneior~ medium
were trastad while living with fluoroooent eubatanoeo: aoridine orange, ~ ~ ,I~ ~;x,
a nd Dori hoe hine+ We observed briefly the results obtained
~ barbarine sulf ate, a P p
with eaoh of thooe aubatanoea~
s utiona 1x100 000--1s200,000~
The acridina orangQ in very weak el ( ,
ee - re�n fluorescence off' the proto-
created in the ~ndor~ycee Magnueii a d p ~ _
L
d a li ht- teen fluoresoenae of the nucleus ~ The vacuole� which
gleam an g g
cumulated a .rest deal of the dye were colored a fiery red. The early
ao g
hen �e not observed under the micr~ecope in white light, were �xpr�eeed
c S ,
her 1 increae�d brightness of the cytoplasm and nucleue~ The prato-
inas PY
plasm began to fluoresce a bright green and the r~ucl�us a yellow graan~
used b an increased adsorptive power of the protoplast~ The
This is ca y
accumulation of Acridine orange in thQ coil in time increased and the fluor-
Decants of the protoplasm acquired a stronger yellow color, became orange
and f~:na~:ly red. The orange �nd red f~.unreacence of the protoplasm coinoidee
with its irrevDrsible damage to it through gDlatir~i~~ation aid c~?agulation~
~ These changes in tt~e color of the fluorescenc� which are connected with
A
maturation of the proteins of th� cell were first observed in plant, cells
d i
r l ~ ~D noted that any disturbance of the cDll which led to its
by Strt~ge (7 ~
t
death was accom~e.nied by t~~e change of tEye fluoreec�nt green color of ~ .
b
red. zn checking these observations ourselves, we
adsorbed acr~.dine ~to ,
s
his than a of the fluor�scent color occurs only for a specific
found that t g
t e of degeneration of the protaplaet and does net invariably accompany
A"
i the death of the cell~A (Meyeel~ and ~avarzina (6)). The type of denaturation
~ ~
` ~ ~ in of the rote laem under the effect oi' ttie cDtyl-zephi~oblxie
'-.A of tk~e prot~ P p
. ~ reed that oharacteristi~~far unusual necrotic pracesse~ leading to .
exp y ~. .
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Table l
i
~ the Abo or tion o~ 0 en b a Cu~,ture o~ ~do~oes
Tnte~eity o A ~
i Maguoii es Z~'luenoed by Va~ous Conoontretio~o os Cet~l�Zephirol~
-.r., ~ rY, , , ~
Conoontratior? o~ Absorption o~ 0 in Oor~ae~ntxation o~ ~ Absorption of 0
i ~illimete~e Cet l�sephirol in oubio millim~tere
Cety~l zephirol oub o ~ Y ~ r hour 1 ~lligran
per hour by l ~ ~ ~ r
. ~ meters ~
milligram o~ dry o~ dry ROZ
material ~
-tt^-'x'~Ct'Ta~tPYt
._.t..,_ .,..--'r-- -.,...P.. --r r.;rt^rniacs.-. a .-.f.-....,mrx-:^rarrYx
I ~
2 . -T
~..,.-.._t_._..t......_. ~
Control b~ ~ 1 ~ 1,000 i 28
I ~ I
1 s ~00~000 68 ~ 1: l~0,000 0 .
1:150000 ~9 ~ It 30000 0
::YE:~..CT::I:Y.'DfT:S::i{t3"ifx. Yl.l', 1:.. ..:Y,'^iw i-: ::.i ....Y~ i i 1 i .t . : 11 r.. iM/N
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Table 2
Intene~Pty o~ Fermdnt~tio~t in ~dom~tcee Ms$nua~li in the Preeenae
of Cetyl-~ephirol
_,Y. r Y t.~.a t
C?)r1t111 t.'o Y�.~_'. .1~tt~~t~ON+..o
noen lM1
I[ C ~ EYOlut~01~ 0~ CO2 ~1 Oub~PO
Cetrl-sepi~irol mtihi~roters per hear b~?
~ ~ ; 1 milligram of dry material Y
~ ~ C QC02~
r ! � u w+s v:u Y lY,.. Hwwrrafw'tf!MNr~1~
,.pYp3?al~Ytl,~p::PNI1p Mpr+++~".Y.�rt Ml Yt ~f: .Jlrt ~lWbrwt/+NYY/I~INfYIWIpM1{~'~+P
i 1_.. a.
Control ~ 4l
1t150~000 40
1:80,000 39~~
1:20~ 000 2b
1s10~0~ 20
.+~n+.,~:b:lwxtr+l�Y-... r�+a,a.�-...,,..+:.Mt Y., .,w....+aa++++Y:wrx,:w.rr.r..~r..+wr.....~.,.aw.t..-I..+;:.:+r�..tutaY:~ta.wa ./t... v:e.e.r~irr. ~I
a 'l
C.Y
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Declassified in Part -Sanitized Copy Approved for Release 2012J05/25 :CIA-RDP82-000398000100100065-9
Declassified in Part -Sanitized Copy Approved for Release 2012/05/25 :CIA-RDP82-000398000100100065-9
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r A ~ �
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f Table 3
~ I
. ~ Intenoity~ o~ F'ermentotian ~dQnq~oee Megnueii ~Ster tho Last er
�ee hirol~ .
hae boon ~,oted upon by Cety~l p
4
Conoontretion o~ L+~olution o~ CO in oub~o
Cetyl�eephirol mlll~me~?ere ~e~i hoer b~' 1
milli6re~a o~ dry materiel
E C I ,I
QCO~ .
a
t 58
Control
1=100000 3~
1t1~0 000 22 I!
~ citr:~.i~ir;::;. ~+e/emb i:c t>./.: 1'.~e n...i~~e+