BLOOD-PLASMA DEHYDRATING EQUIPMENT

• Document Type: CREST
• Collection: General CIA Records
• Document Number (FOIA) /ESDN (CREST): CIA-RDP83-00418R002000100009-5
• Release Decision: RIPPUB
• Original Classification: S
• Document Page Count: 4
• Document Creation Date: December 22, 2016
• Document Release Date: June 20, 2012
• Sequence Number: 9
• Publication Date: December 20, 1955
• Content Type: REPORT

Declassified in Part - Sanitized Copy Approved for Release 2012/06/20: CIA-RDP83-00418R002000100009-5
INFORMATION REPORT INFORMATION REPORT
CEN1RAL i 1tEtL tNCE' AGE 1-CY
This material contains information affecting the National Defense of the 'United States within the meaning of the Espionage Laws, Tit.1,
18, U.S.C. Secs. 793 and 794, the transmission or revelation of which in any manner to an unauthorized person is prohibited by law,
S E=C $ E-T
~tt~FOT~N
COUNTRY Czechoslovakia
SUBJECT Blood-Plasma Dehydrating DATE DISTR. 20 December 1.955
Equipment
DATE OF INFO.
PLACE ACQUIRE
DATE ACQUIRE
NO. OF PAGES 2
REQUIREMENT NO. RD
REFERENCES
SOURCE EVALUATIONS ARE DEFINITIVE. APPRAISAL OF CONTENT IS TENTATIVE.
1. At the end of World War II, US blcod-plasma'dehydrating equipment was
given to Czechoslovakia under UNRRA and w. as sct up in the Medical Research
Institute in Prague-Vinohrady, in the new hospital in Hradec Kralove
(N 50-13, E 15-50), and in the periicillir.factory in Roztoky (N 50-10,
E 14-24). After 194, no more C` NRRA-,eequigmenL of this kind was supplied
and Czechoslovakia started her own production. At the request of the
Ministry of Health, the task of producing a prototype for the apparatus
was entrusted to the Research Institute for`Refrigeration in Prague-
Smichovl and was to be manufactured by the Frigera National Enterprise in
Kolin (N 50-02, E 15-12). One original piece of equipment therefore was
lent by the Medical Research Institute to the Research Institute for
Refrigeration. There it was copied. The first prototype made was absolutely
identical to the US model.
2. Difficulties, however, arose with measuring apparatus and mechanical
equipment, since these Czechoslovak products were far from the US
products in quality. For example, a compressor of the same output (e.g.
- 60?C) was about double the size of the US one. An air pump for as low
pressure as 14 microns was not available at all and had to be put into
production at some factory2 in Roznov pod Radhostem (N 49-28, E 18-08).
Low vac.nmi auges likewise were not available and their manufacture was
entrusted to the Metra Works at Blansko. Since the system works on a
very low vacuum, production procedures were not known and at first this led
to a considerable lack of airtightness in the system. The demand was made
very suddenly and the plant had to go into production quickly. Because
of the shortcomings' mentioned above, the completion of the equipment was
much delayed and considerable difficulties arose in negotiations with the
ministries. Since the apparatus itself was already in existence,
production was hampered only by sub-deliveries from the factories mentioned
above. The apparatus was eventually handed over to Frigera, Kolin.
3. The equipment is for general use and,may be employed by any blood
transfusion station or hospital. Research work on blood plasma was
carried out in Prague-Vinohrady and production of blood plasma on a large
scale was carried out by the penicillin plant at Roztoky. In addition,
there are at Roztoky large rotary dehydrating plants for blood. Production
is supervised by Dr. Majer (fnu) and Dr. Sterba (fnu).
LX_
S-E-C-R-E-T/NOFORN
- AIR x FBI AEC oCIi
(Not.i Washington distribution Indicated by "XIi Field distribution by
FORMATI.ON REPORI INFORMATION REPORT
Declassified in Part - Sanitized Copy Approved for Release 2012/06/20: CIA-RDP83-00418R002000100009-5
 Declassified in Part - Sanitized Copy Approved for Release 2012/06/20: CIA-RDP83-00418R002000100009-5
2
S-E--C-R--E-T
NOFORN
Description of the Apparatus 50X1-HUM
4. The apparatus for dehydrating blood plasma consists of two airtight drums
joined together by a pipe. One single-stage pump is used for the whole
plant to pump out both drums to 15 microns on the column of mercury.
A single-stage compressor is also used, creating an evaporation temperature
of -60 C. There are instruments for registering on a switchboard pressure
and temperature inside the apparatus. Since very low pressures and
temperatures are involved the registering instruments urate
and are very expensive . 50X1-HUM
Production Procedure
5, Human blood is left to settle for several hours and the corpuscles separate
from the plasma. The separated plasma is then drawn off, filtered, and
marked with the blood group from which it came. After filtering, it is
poured through calibrated measuring vessels in amounts of 250 ccm. and
300 ccm. and into glass bottles for further processing. The bottles are
closed with special rubber stoppers and placed horizontally on a special
rotating apparatus in a bath of alcohol at a temperature of -300C. The
bottle rotates about 10 times per minute and as it rotates the lower part
of the bottle is constantly in contact with the frozen bath of alcohol, so
that gradually, after about 48 minutes, the whole plasma content is frozen
to the inner walls of the bottle. The frozen plasma is then uncorked, put
into a metal basket and placed in a sublimating boiler. The boiler is
hermetically sealed and refrigeratior proceeds by means of a pump. After
about 10 minutes, pressure sinks to about 200 microns in the mercury column,
and all the registering apparatus is connected. After about an hour, when
the position is stable, the sublimating heat is turned on and the process
itself is watched by a doctor or a research worker on the registration
instruments. It lasts about.23 hours and then the instruments show the
end of the process by a sudden drop in temperature. The research worker
then stops the apparatus, breaks the vacuum, opens the sublimating boiler
and takes out all the containers with the bottles. The bottles are then
quickly removed from the containers and corked again. This is done in
special rooms under strict hygienic conditions. On completion of the
process, the heat is turned on in the condensing drum, the frozen water
melts and is driven out by a hand ventilator. At the end of the process,
the apparatus can be used again immediately.
omment; This institute 50X1-HUM
ent name . State Research Ins ute for Refrigerating and 50X1-H U M
oo n uliances.
2. Commente this 50X1-HUM
is probably the Tesla- oznov National Enterprise , Plant 01, at Roznov 50X1-HUM
pod adhostem.
Attachment (1 diagram, 1 page of legend):
S-E-C-R-E-T
NOFORN '
Declassified in Part - Sanitized Copy Approved for Release 2012/06/20: CIA-RDP83-00418R002000100009-5
 AZI ores:
Declassified in Part - Sanitized Copy Approved for Release 2012/06/20 CIA-RDP83-00418R002000100009-5
.~. brass c lindrical condens ._drum, 400 mm in diameter, 900 mm high,
walls about 2~ mm thick, FM_;n the outside with airtight lacquer
(composition not known), 50X1-HUM
29 Cylindrical sublimating boiler, dimensions the same as (1), with
removable Tid. There are four basket containers inside this boiler,
each for six bottles, containing 400 ccm, and 75% filled with filtered
blood plasma. When the plasma has been frozen to the inside walls
of the bottles, these are put into special baskets and placed in the
sublimating boiler which is closed hermetically with a strong lid,
attached by about 8 wing nuts.
3a Pipe.. connecting the two drums, 90 mm in diameter, brass, wall 2.5 mm
It is welded with. silver solder.
4. ppiralevaarator, acting as a condensing tube for steam sublimated
from the plasma.
5. Automatic thermostatic expansion valve, SAUTEFt type, 1.5 mm.
6. Single-stage compressor, FRIGERA, 4,500 Ccal/per hour, evaporating
temperature -10?C,
7. Water-cooled condenser, reverse-current.
8. Electro-motor, driving the compressor, 350 Watt.
Single-stage rotary pump. of American origin. Certain spare parts were
request for mass production of the STRUMIA apparatus, and among these
was the pump. For the prototype the original American pump was used
since there was no identical Czech product.
10. Temperature regulator, insulated.
11. Metal late for alcohol bath where the glass bottles containing blood
Plasma are froen on rotating apparatus.
12. Switchboard, with switches and registering instruments.
13, Baskets for bottles, consisting of a round metal belt in sections,
the walls about 1 mm thick, On the inside there are spiral spring
holding-devices for 6 glass bottles.
114. Electric heating iPmRn1 , about 100 Watts for supplying the necessary
sublimating heat for the operation,
15. Electric heating element, about 400 Watts. When the operation is
completed, this element is switched on to melt the ice on the condensing
tube.
16. Hand-operated vaccum valve. During operation this valve is firmly
closed and is used--+- ei o
t th
u
e water melted off the condensing tube,
17. Holder for thermometer, showing the temperature of the spiral evaporator.
Declassified in Part - Sanitized Copy Approved for Release 2012/06/20: CIA-RDP83-00418R002000100009-5
 Declassified in Part - Sanitized Copy Approved for Release 2012/06120: CIA-RDP83-00418R002000100009-5
Blood Plasma Dehydrating Equipment
Declassified in Part - Sanitized Copy Approved for Release 2012/06120: CIA-RDP83-00418R002000100009-5