SCIENTIFIC ABSTRACT FR:KOTELNIKOVA, A.V. TO:KOTELNIKOVA, V.P.
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CIA-RDP86-00513R000825300013-6
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SCIENTIFIC ABSTR
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savJ2a-~?a-~.-52/5a
Separation of Adsnylatkinase and Nucleos:lde Monophosphatkinase From the
Prater-soluble Fraction of the Live~~
experimental methods were the same ae in reference 1. The fol-
lowing oonolusions may be drawn froca the investigations. Thf~
enzymes adenylatkinase anil Numoki could be completely sep-
arated by fractionating the aqueoum liver extract of rab6it~~
by means of alcohol. Adenylatki.naee is contained in the frac-
tion of a-2a~ at~d Numoki j.n the fraction of 4a-5a~ oi: alcohol.
It pas for the first time that these enzymes could be separated.
Adenylatkinase catalyzes the phosphate transference only among
adenine nueleotidse. Numoki apparently catalyzes this reaction
betpeen nucleoside tripho~-phate and nucleoside monaphosphatel
under the ti~lloring conditions 1) Nucleotides of every coulllr~
have to contain different bases; ~) one part of every
nucleotide couple has to be an adenine nucleotide. Enzyme
preparations from hog kidney (Ref 4) and calf kidney (Ref 5}
showed the obligatory participation of adenine nucleotides in
the numoki reaction. The diets found prove these conclusions
also pith regard to water soluble Numoki from rabbit kidney.
There are 3 figures, 3 tables, and 5 references, 3 of which
Card 2~ 3 are Sovie t
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17(3} ~OV/Za~~ze~~~-yz/5c~
AUTIiORS: Dovedova, Ye. Lo, Kotel'nikova, A. V.
TITLE: Separation of Adenyl;~tkinase and Nucleoside P~~onol5hosphatki:xa~3e
Froru the Water-soluble Fraction of the i,iver
PEItTODICAL: Doklady Akademii nauk SS;iR, 1g5y, Vol 125, Nr 1, pp 1~~~19'j
(USSit)
ABSTRACT: The present paper gives clata on the preparative separation of
the enzymes mentioned in the title. The reparation was cau>rjed
by fractionating aqueous liver solutions by means of alcohc-I.
'i'he substrates u�ed were; adenar~intriphosphate (ATP) and
adenosin-5'-phosphate (AF'P), purified in rectifying columns
from commercial ATP by means of anionites AV-16 or AV-17;
uridin-5~-phosphate (UFy), produced from yeast and purified by
means of AU-16, as tivell a,~e imported; inasinw~~ ~phoaphF~te ~IFI'~,
guanosin-5~-phosphate (Gl='P), uridintriphos~hate (UTk) - im-
ported goods for sale; inoaintriphoaphate (ITF'), made available
by V. Z. Gorkin of the In.stitut biologicheskoy i meditsinsk.oy
khimii AA~N SSSR (Institute of Biological and ~~edical Chemistry
AMS USSR). The reaotion to nucleoside monophoaphatkinase
Card 1/3 (numoki) wF~s mainly investigated by means of UFP and ATP. The
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On the Specificity of the Water Soluble "Adenylata" SOV~2G-123-2-33/50
Kinase From the Liver
showed any activity. It nigy be ooncluded from these results
that in aqueous extracts #'rom the rabbit liver the "adenylat~e"
kinase (speoifio for adenine nuoleotid�s) as w�ll as the
numoki are contained. There are 3 figures and 12 referenos$,
3 of whioh era Soviet.
ASSOCIATTQN: Laboratoriya fiziologiehaskoy khimii Akademii Weak SSSR
(Laboratory of physiological Chemistry of the Academy of
Saienoes, USSR)
PRESENTED:
SUBMITTED:
Card 3~3
July 2, 1958, by A. I. Opa.rin, Aoademioian
June 26, 1958
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On the Specificity of the Water Soluble "Adenylate" SOY~20-123_2-33/ 5a
Kinase From th� Liver
(like numoki). With the yeast enzyme it was proved that
numoki is different from adenylate kinase. With liver enzymes
this proble>!m remains unsolved. The authors investigated tl'ais
problem in aqueous extracts from rabbit liver according to
the method given in reference 1. Figures 1 and 2 give the
expeximental results of the activity determination of the
"adenylate'" kinase and numoki in one and the same liver
extract (treated with FiCI). The reaction products were
separated by paper chromatography and quantitatively determined.
From the results may be seen that the watex� soluble liver
1'adenylat�" kinase is, like the myakinase, a specific
"adenine" enzym� and differs from numoki. Figure 3 gives
the results of the activity determination of numoki after
the heating ofd the dialyzed aqueous liver extracts for 2
minutes at 60 and at pg '] (R�#' 12), The numoki reaction
was clearly shown. The negative numoki reaction in �xtraat�
treat�d Kith HCI were explained by the higher re$istanae of
the numoki to heating in ~leutral medium compared with that
Card 2 of tho "ader~ylate" kinase� On a heating of the extracts up
~3 to 80 for 2 minutes at phi 7 neither of the two ferments
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17(3)
AUTHORS: Kotelfnikova, A. V. Dovedova, Xe. L. S4V/24-123-2-33~'Sp
TITLE: 4n the Speoifioity of the Water Soluble "Adenylate" Kinase
Fxom the Liver (0 spetaifichnosti vodorastvorimoy
adenilatkiraazy iz pecheni)
PERIODICAL: Doklady Akademii nauk SSSR, 1958, Vol 123, Nr 2, pp 327
334 (USSR)
ABSTRACTS In earlier papers of the first mentioned author it was proved
that th� animal liver contains the farm+ant (phoaphomutase
ADF) mentioned in the title, which highly differs from that
from the muscles (myokinaee) (Ref 1). Since in living
organisms free nualeotide~s were found together with others,
as adenine, purine and py:rimidine bases (Ref 2), in animal
tissues and in yeast enzytnea (Rafe 3, q) were found that
were called nuoleoside-~mo~aaphosphate kinases (abbravis,;tedi
numoki). They catalyzed the phosphate transfer from
nucleoside triphosphate to nucleoside monaphosphate, haweve;r,
with different purine- or pyrimidine bases. In this con-
nection the question arosE- if the water soluble liver
"ader~yiate" kinase ie a e~>eoifia "adenine" enzyme (lik� the
Card 1/3 myokinase, Ref 3) or if it is specific within a wider range
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USSR; Human and Atlimal Physiology .. 2~~~tabalista. - T
' Abs Jour Rif Zhur Biol . , 1Vo 3, :959, 12531
which possessed a hig~i s3pectfic radioactiivity. Hy a
studf of the propertieES of this substance it was es-
tablisiled that it CpntLtined $--Sgroups ui~d the proper-
ty of coenzyme A ~t'~e e~bility to catalyze acetylized
anino-a7.obellzene. ~a~he recovered substance was different
from phospha>"yl coenzyme A 111 that it did not vie a po-
sitive reaction on the free SH-groups after treatment
with alkali and had a Ciefinite Mobility with paper
crlror:.atography t~sitig N-propanol--NH3--H2O as the solvent.
-- V.I. RosPt~gart
Card 2~2
. 21 -
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USSR/'Hw:gal: aid .Lli:~i~a. !~Yaysi.a~.c~gy - Metabalisl:t. T
� ~ Aar Jour Rei X~hur Riot.., era 3, 1;~5~, 12531
Author : Kotel'nikova, A.V.
. ~~
Fast ; A5 USSR
Tittle Role of Metabolites of F"senzi~ A in Phosphorus
MQtabai.siri of Liver of Ar3ir~aly ,
Orig Pub V sb.: ]:z~~ch. zhivotn. organizr~~a. M., AN SSSR, 195,
83-95
Abstract ; Rats were in,~ectcd With phosphate P~2; after 15 3a
riita~:t~.s the an~,m~1F were sr~cri-(iced, the livers were
rerwved, and After prec~lpitat~i.on off' the protein with
trichloracetic acid the fra~ctio~r of Ba-no~~-soluble
phospharus co~ipoalds wa;otel'aliknva ;Anri V, U~ aPYr'$se in Po A
1s!ly~Aknd llydrtk'�~~~~.5;~ ., ~, 5aiornatinn. Duk�
cnncerr~ccl a~ith trlrrtsf~ttls,itat g~~rl ~acle -~it hhosphu~to,~,
ttuttteiy (tnYro~; un,t Irt~Ic) pllu~~shrlt~lfic, wei~ ,
t ttti~crls nt ~urlau~ vur,etli~s o! tidttltges,.. A ntntt, in the
terem~e Irt the ~nayrttic nctivltiex nt ~. ectaeki~ruble dU~
taitgti, lnclicatLig A` l~ptieuiar! ~`ttrictlea studio was
ucttvtty ut the sitr ~' itnportunt mk M apYrnsa'
sh~rch xyn hesi~ tsk~ iiu~r r ~evrtgpraent at winch intefta;ive
i . Cen~ tasJl~j the Y~rietles with
lase s~itrc~h tttertt hav~iaw apyt~se act vlty,
starch turttla shviv ~! illb'}j activit o whlk the high.
A htttas> ttttivIt it/A3 Y l the entyme, ~
vim; y es~entittlly the $ame lp 6111
i
~; ~
!,+::
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~ttTFL!pIR~Vd, ~. ~.
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a3,a~.pgiaal. 41~mi a ter
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1~ ~ puadoa aaa propett[~~ a
~~ ~ir~-~
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vat ~xts , in thg a-1t118 v~ai.
m ne, ~n ra41 nau. no etreet do tha t~tivity o~ li, ~~'he ttoi115ed
p~"ocedurd aiforda a 12-I~�foid putificatiat accompanied ~y ~
a 2~-~2-foicf'ccnwen, itt tht activity df the I. Unlike the nc~a�
pttritlecl pct. the ptirlliod I is anatabie_in mcdltt ~ acid dnd
neutral ccactlojn~ and i:E mote cagily insctixatett` by CCi~.
C~al~t. Tits tttability of I in ndnpudtjal recta, is d
Rprclfla ataltill:er- a>ut not to tuty atruc~tttcal dli'F ~>.+
i-~ta activptrn I up td OU-96~. B� 8. Imo'
i~
1:,
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KbI'EL ~ N'~K4VA ~ A. V,
tiSSR/Biology {Agrizculture) -Starch From Potatae8
Sep/Oct ~,
~~Starah and xta Formation in Fotataes,n B. N. 5teparienko, Ye. L, Rosenfeld, A. N,
Petrova, A. V. Kotel~rikava, Moscow
'~t)spekh Sovrem Biol~+ Vol XXXII, No ~ pp 193-'31
Potatoes are s very important crop in the USSR; 7.7 million hectares were planted uru~er
potatoes before World War IT and the acreage was ~~ higher in 1850, Yield from 1
hectare corresponds to 1,600 liters of alc, which may serve as raw material far synthe-
tic rubber, While yields were raised by 21$ during the past 10 yrs, the starch content
i.s often inadequate. A number of interesting investigations on starch formation in
potatoes was carried out at the Inst of Biochem imsni $akh, Acad Sci U5SR. This wank
and other data will help in raising the starch content. Reviews in detail the present
status of the problem of phytochem starch formation.
198T1
p
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~Oi'KOYA d. P.
-_~--w--~-..~,.~..,..~_.___.,
6529� RCOTBLNIKOPA A. P. Machan~isd oP transfer et the adenylic system of high-anerg~-
phosphates in yeasts Dckladi /Yade~aii Hiwk SSSx, Nesaaw 1950, 69/6 (83q-840) Tables 3
'.Pwe pssslble traneperting aechaaisns �f high-oa-ergy phosphates
in yeasts: (a) A phasphemutae� ~' be sup}�sed to ecL
kb) an en ~ utilise adea~pliq acid (i~aatdad et ADP ar ATP);
t~yme system, stiXl unknown, ~ exist in the yeast sell, ade~rlic acid playing
the role of a phosphate aoaeptor and dDP that ~-f a phosphate decor. d phosphemutasa
~~ould require the presence �f hexekinaee to 11u-ctien as an ensyme system; direct
aaperimontal evidence ter a phosphomataae-transporting mechanism was obtained by eepa-
ration at the two enzymes in a ;~eaet extract, by 3 minutes' boiling of the neutralised
solution, which reduced hezokin+sae activity to ~ of the �riginal value, whereas the
X-hosphoa~ntasa activity was redu~aed only to 61~. The tact that yl~ast entracte could
split phosphate e!P ADP in pros,�nae of myosin m>ay be regarded as further evidesoe In
tavour of a phosphomutaee system since purified myelin splits only one equivalent of
phosphate from ATP and does not split ADP at all. Magnesium ions were toned to eat as
efteators et yeast phosphamntaea.
Zagreb
S0: I~xcerpta Modica, Sectiea II, Pel III, l~0 12
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7~n,3_~...~a~~8
Inveratigatian of the Campl~ex Compounds of Bivalent Rhenium
. compounds of other valence t'rie a xtraotian method from aqueou~~
hydrochloric solutions witr~ organic solvents was ~tsed4 In
this it wag :Pound that the best extraction of Re '" Can be
carried out'with such orgaa~ic solvents in which C 0 occurs(;
logo pinacol;iney aoetophenone,, methylacrylate~ dimethyl=
phthalate and otherso
Some bivalent rhenium compounds were isolated with methyl
alcoholo ~n the reaction of H2ReC1 e 2 H2O in glacial
acetic acid solution the followin~4compounds form on addi"
tion of methyl alcohols
.. Recl2 0 4 cH;scooH
ReCl2 0 2 CH.~COOHoH2O
ReC12 o CH3C()OH o H2Q
ReCl2 4 CH~COO~i
On the action of ReCl2 ~ CIt COH with pyr~din� ~: ao11d sut,...
stance of the following cora~osition is formedR ReC12oCH3
COL~H.py,
Card 3~4 'rhe thermogxaphi.o investigat~ione of the derivatives of
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Inveati~ation of the Complex Compounds of Bivalent Rhenium
of ternperatt~re it dscompaeea fo~ming oxidation products,:
ReC~.~ o ~ Hi?Q on heating; to 1~0 C oleo looses 2 mol water
forming ReC:[ n2 H 0~ In theaQ two compounds the water can..,
based on thE:2experimental .resutts4 be regarded as coordi~~
natively bound, 'She formula is given as follows:
~~ReC12 n(H2Q)2 l a 2 H,,Q and
~.Recl~ (~i2a)2
H ReC12 ~ 2 H2O undergoes some intermediate compounds with
the beginning of hydrolysis; these intermediate com~ounds .~
were also i;~olatedin solid forma Hz~Re(OH)3C1~ H2~Re(OH) J ~.
4
ReCl2 ~~ 4 H2Q and ReC12 ~ 2 H2Oo
A1eo some ac;idous salts were isolated in solid forma KHReCIQ�
o H2O9 KHReC14~ NH~HReC14 ~ H2Q and NFi~HReCl~b (PyH)HReC14o
In the treai;ment of H ReC1 �Q 2 H2O PyH HReCl!~ forma with
pyridine in cidous e~iumo4This compound can 2asilY be con�-
vented into ~Re02Py C1 in the evaporation in air Re�II
transforming ~.nto R �~=V on this occasiono
Card 2~4 For the purpose of separating bivalent rhenium from rl~ienium.
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7~..3~~_3q~3~
AIITiiORSs Kote1�nikova9 Aa Sr.g Tronev, V~ Gp
TI~~L~s' ~ igatio;n of the Ca-uplex Gompounda o,f Aivnlent Rhenium
(Issledovani;y~e kompleksnykti aoyedineni y dvukhvalentnogo
reniya)
Y~RIODICALs ZYiurnal Ne~organicheskoy Kh:Lmii~l~ja,Vola3,I7r ~,ppo100S~~10?j
(USSR)
AIISti'RACTa The Complex compounds of rl~enium-II were produced and invest:i�-
gateda From the experimental material followss ths,t by the
reduction of KReO in concentrated hydrochloric acid solurv
tion with gaseo us4hydrogen and under a pressure of from
100 ~ 110 atmospheric excess pressure and at 300�C solutions
can be produced in which rhenium exists in a bivalent form
From the hydrochloric acid solutions of ~ enium-II with
acetone solution the following products were isolated by
crystallizations ReC12~q.H2O and H2ReCl 02 H20~
Thermographic analyses were carried ou~ with these compound s
ReCl2 b 4 H2O has two endothermal effects at 150�C and 398oC~
Card 1~4 H2ReC1~ 0 2 H2O 1c, ses wat~ex at 15OoC and with a further rise
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f
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1
1
AP603341g ._._._.__.._._____-------~.,__~.__.~.,.__.. ~_-.._._..... __... _.__-
difforent opQrating conditions: the dust ~ ...__~__�_..._ .._.__.~._ _._.__.~.~,~ _._.~____.__ _....~
the magnetic field strength rant ian of thc.~ discharge was 12 '
varied from fi bed from 12 to 25 kOQ� �r 20 millisec�
x 10`5 the initial h ~_
to 4 x 10"~ torr, and Cho linear tempei'atuz�a wasrvurn pressure
20 And 500� C. Some of the data are Irz�egent;ed r ,
the aid of the l~zaphicall a ied between
matQrial balance equation a y nd are discussed,
lifetime of an ion in the ~ teclu:iquo is clQyelopod fox� deriving`ViChe
measurements of plasma densitymandrradius ofs pr�s5ura measurements
assumed cross s�etions i'or cl~argQ exchnrtgo andhioniznLa filament a' th'a usua?
the plasma. The ion lifetimes was found to i ion of atoms andtmoleicule c-r
strength and to be vc ncreasc With sin
ry sensitive to other characteristicsnof ethcng magnetic field
gation of these relationshi s
eomowhat less than the durationso~eing continued. Plasma. Invosti-
conditions, and the plasma densit w The ion lifetime was found to be j
plasma confit1ement under all the investigated
ion lifetime and the lifetime of an absorbed atom aAb~ut equall
3 formulae and 10 figures. Y determined by tht;
ho linear. Orig. art. bees
SUB CODE; 20
Card 2~2
SUBM AATI~: 1~Nov65
ORIG. REF: 010 0TH REF: 007
~' ~
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SOURCDs 'COpK: Un/0057/kiG/(}3G/k)10/1$.~ /1&1:1
s
E AUt'll0i~: Garbunov,Yo. p. ; Kot,el ~ nikov,Yu.N. ; Kutukav,G~ p, ; Simoncv,V,A.
._.m...~u.Q~-
OitG: none
TITI.K: Investigation of the material balance betwaan tha plasma :filament and tbo
gaseous shall in the Tokamak-3 toroidal machine
SOURCK: Zhurnal tekhnicheskoy Sizilti, v. Ski, na. 10, lAGki, 1f~31-�1841
TOPIC TAGS: hydrogen plasmas plasma confinE:ment, gas pressure, ionization gage,
gas absorption, ion lifetime
ADSTRAGT: The authors have employed specially designed ionization gages to record
the pressure of the neutral ryas surrounding the plasma filament duz�iiig operation oP
the Tolcama}c-3 machine, and with the aid of the data thus obtained they discuss tt-e
exchange of material between the plasmn, the surrounding gas, and the stainless steel
linear of the toroidal discharge chamber. The electron beam of the ionization gaga
was modulated to make it pos;,ible to distinguish the ionization gage signal agair-st
the noise background from th~~ main discharge. Kash instrument consisted of two
identical ionization gages in a single envelope; one gags of the pair was usually
operated with a cold cathode and its signal was subtracted from that of the normE-lly
operated gage to reduce the noise background. Entrance of charged particles intc- the
ionization gage was prevented by an electrostatic trap. Data were recorded undez�
Card 1/'2
UDC: 533.9
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APPROVED FOR RELEASE. 06/23/11 CIA-RDP86-005138000825300013-6
The Isotopic Effect in the goV~~56-59-26/48
ethylamine Mutual Solubility of ~Yater and Tri-
with the measuring results. An isotopic exchange occurs in
solutions of triethylamine and heavy water Under exlaerimental
conditions (concentration of triethylamine 35`~ by weight)
1 y 1.5~ of the deuterium atoms passed over into triothylamine.
There are 1 figure, 1 tablla, and 3 references, t of which
is soviet.
PRESENTED B7Cs� Kafedra teoretioheskoy khimii Ural'akogo palitekhni
instituta im. S. l~1. Kirova eheskogo
(Chair of Thr~oretical Cherai.stry, Ural Polytecl~nic Institute
imeni S. M. Kirov)
sUBMTTTEfl: SQptember 22, 1y~8
Card 2~2
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21(1) 5(4) ~ov/~5~-~59-2~6/4e
gOTHORS: Kotel'nikavg .,'V,_,_vV., Skripov, V. P.
TITLE: The Isotopic Effect in the i~iutual Solubility
of Water and Triethylamine (Izotopnyy effekt vo vzaimnoy
rastvori,tsl~nosti vody ~. t;ristilumina)
PERiODICgL: Nauehnyys doglady vysshey ;~hkaly, Rhf.miya i khimicheskaya
tekhnologiya, 1959, Nr 2, pp 248-249 (USSR)
ABSl'BACT; The solubility in the systlams (C2H5)3N - D20 and (C2H5)~1P- H,~4
is investigated. A diagram (Fig 1) chows tha experimental
results. Tha solubility is reduced in Y~savy ~vatex, the
oritioal tearperaturea amaurit to 1'x.86 for H2O and to
14.05� for D20; thus the difference is negative. For dif-
ferent deuterium content off' the water the change of the
critioal temperature in the aria of an equal concentration
of Height of triethylamine is calculated in the falla~ing
xay: tx ~ tH~O w o.x(tH2O .. tD20) (tFi20 9 tD20 denote the
~.
temperature :limit values at 100 H2O and D20, respectively).
Card 1/2 6 table gives the values for cx. They are in good agreement
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