SCIENTIFIC ABSTRACT KRASILNIKOV, N.A. - KRASILNIKOV, N.A.

• Document Type: CREST
• Collection: Scientific Abstracts
• Document Number (FOIA) /ESDN (CREST): CIA-RDP86-00513R000826110008-5
• Release Decision: RIF
• Original Classification: S
• Document Page Count: 100
• Document Creation Date: January 3, 2017
• Document Release Date: July 31, 2000
• Sequence Number: 8
• Publication Date: December 31, 1967
• Content Type: SCIENTIFIC ABSTRACT

-/./ -) C., -
I -; - ,, /V X).
KRASILINZOV, N.A.-,.SIMYABIN, G.K.
-'.
Development of studies on antibiotics through research performed by
Soviet microbiologists. Antiblotiki 2 no.5:3-7 B-0 157. (MIRA 10:12)
1. Institut mikrobiologii AN SSSR.
(ARrIBIOTICS, I
research in Russia (Rua))

KRASILINIKOVO H.A.; XORENYAKO, A.I.; 1,MKSLITA, M.M.; VALOINSKAYA, L.K.
USSLOV, 14.14.
Culture of Actinomyces No.111, Actinomyces luridus nov.sp., roducer
of the antiviral antibiotic luridin (with summary in RnglinX
Mikrobiologiia 26 no-5:558-564 S-0 '57. (MIRA 10:12)
1. Institut mikrobiologii All
skiy institut antibiotikov,
(ANTIBIOTICS,
luridin, prod. by
properties (Rus))
(ACT INOKYCBS 0
luridus, prod. of
SSSR i Veesoyuznyy nauchno-iseledovatell-
Moskva.
Actinomyces luridus & antiviral
antibiotic luridin (Run))

N.A.
Of noll micro-organisms in plant nutrition; from material of
Soviet mtorobiologists during the past 40 years. Mikrobiologila
26 no.6:659-672 N-D 157. (MIRA 11:3)
.(WIL-HIPRO-ORGANISMS) (PLANTS__M~PRITION)

EKMKIA IMICA Sec.4 Vol.11/1 Med.Microbio.etc. Jan 58
RI) 5 1 I_ N I KO V, N - A , - - - I ~ ~ - .. I . ~ - - . - - ~ - I -.- _.
10. KRASSILNIKOFF N. A. Inst. de Nficrobiol., Mo5cou. La claisification des
actinoinyckcs producteum d'antibiotiques Classification of actinclinycetes pro.
ducers of antibiotics Ann. Inst. Pasteur 1957, 92/5 (597-604) Tables 3
The classification of actinomycetes producers of antibiotics is dillicult, in the first
place because of our insufficictit knowledge of thei- morphology, secondly be.
cause of the great variability and polymorphism of the species they belong to.
Tlic author suggests a new classification Method, based on the antibiotics pro-
duced by these microorganisms, The antibiotics may in fact be considered as
absolutely necessary to the struggle for life between rivals; they manifest their
activity towards competing bacterial species only; they never show any activity
against cultures of the same species. Generally all the strains of one species pp-
duce an antibiotic inhibiting the growth of all the strains of the rival species.
The author gives several examples of this phenomenon. The antagonism between
species may be uni- or bilateral. The narrow slecifit of antibiotics produced by
various species can be used in taxonomy for e di erentiation of these species.
By means of crossed antaFoni;m experiments, the author has succeeded in dem-
onstrating the heterogeneity o many species considered till now as homogeneous.

20-117-5- 47/54
AUTHORS: Krasillnikov, N. A. , Corresponding Member AS USSR, ~,Lnd Kotelev,
V.
TITLE: qualitative Determination of Phosphatase Activity in Certain Groups
of Soil Microorganisms Nachestvennoye almdeleniye fosfataznoy ak-
tivnosti nekotorykh grupp pochvennykh mikrooriganizmov)
PERIODICAL; Ddklady All SSSR, 1957, Vol- 117, Nr 5, PP- E394 - 895 (USSR)

ABSTRACT: The opinions concerning the problem of the exploitation of phos-
phorus compounds by plants are divergent: according to some auth-
ors organic phosphorus compounds can be directly absorbed, accord-
Lig to others, however, they have to be di3integrated up to inor-
ganic phosphorus (reference 2). The microorganisms as well as the
fermentative systems of the plants are actively takint- part in this
latter process (reference 3). The microorganisms washed out from
the root-near soil have an onL- weak amylolytic (starch splitting)
activity. On the other hand, invertase as aell as ai.Vlase exist in
the roots of sterilely grown plants (reference 4)- The enzyme
group of the phosphatase has a fu-ndamental ijaportance in the de-
composition of organophosphatases and takes part in the biodynamics
of the soil phosphatase. This can also be effected by biocatalysts
Card 1/3 and enzymes of bacterial origin, even in the case that the soil

20-117 -5-47/54
Qualitative Determination of Phosphatase Activity in Certain Group3 of Soil
M
2
icroorganisms
does not contain any increasing microorCanis:as (reference 5). Ac-
cording to the kincLof the soil and the phosphorus compound this
process takes place more or less quickly and the decomposition has
various degrees. In contrast to other researchers (reference 10-12)
and former works of their own the authors used plienol-phtalein-
-phosphate (reference 13 - 14) for the solution of the problem in
questionv There is a colored reaction in precence of phosphatase
if it is splitted into free phenol-phtalein and phosphorus by these
latter. Phenol-phtalein molts red in a buffer solution. From the
intensity of this color and from the width of the colored zone of
the culture medium around the microbe colony it can be concluded
to the quantity of phoophatase separated by the different micro-
organisms. The preparation of Elie culture mediuj;i is described. Se-
veral species of bacteria, fun,-i, actinomycetes, Acotobneer, and
cellulose disintegrating bacteria served as experimental material.
The width of the red colored zone around the colony, expressed in
millimeters, speaks of the fact that most soil microorganisms have
the capacity of producin- phosphatase. Besides ammonificators
Pseudomonas species, and varic-a soil fungi which have a consider=
able phosphataae activity also the cellulose decomposing bacterium
Card 2/3 sorangium has this capactiy. The qualitative reaction suggested

20-117-5-4T/54
QUalitative Determination of Phosphatase Activity in Certain Groups of Soil!
Microorganisms
here can be used foi~he detection of the most active soil microbes
with regard to tile phosphatase production in order to be able to
use them as bacterial manure the effect of which is based on the
mineralization of organophosphatase in the soil. There are 1 table,
and 14 references, 6 of Yihich are SlaAc.
ASSOCIATION: 'Institut mikrobiologli Akademli nauk SSSR (Institute of Microbiology
Of the AS USSRJ Mo,1dWMk1y filial Akademil nauk SSSR(Moldavian Branch
of the AS USSR
SUBMITTEDt September 21, 1957
Card 3/3

KRASILITIKOV) Nikol" A.
Institute of Microbiology, Acad. Sci. USSR.
.. Biosynthesia of Antiviral Substances of the Actinomyces Origin."
paper presented at Seventh International Congress of Microbiology, Stockliolm,
Sweden, 4-9 Aug 158.

KRASILINIKOV, Nikolay 1,Al eks,andrQv Ich; K&TSNELISON, S.M.. red.; SAVCHNIIKO,
.~tld --~Ga --
kbn,r .
(microbe activators and plant IV93 Mikroby-aktivatory I zhIzn'
rastenti. Moakwa, Izd-vo "Zonanle," 1958. 39 P- (Vseeoiuino8
obahchostvo po rasprostraneniiu politicheskikh I nauchufth snanii.
Ser. 8. vyp. 1 no-23) (MIR& 12:2)
iR~izoeperi microbiology)

rRA ILINIKOV,.Nlkolay Aleksandroviche prof.; 5SYMSEVA, M.G., red*;
----k,,WADANOVA, L.A., tokhn.red.
[Antagonism of microbes and antibiotics] Antagonizm ml obov
i antibioticheskle Yeshchastva. Moskva, Gos.izd-vo 'Sovetakaia
naukao* 1958a 337 P, (MIRA 1215)
1. Institut mikrobiologii AN SSSR (for Irasillnikov).
(ANTIBIOTICS) (BACTICRIAL ANTAGONISM)

DASILINIKOV, Hikolay Aleksandrovich; POTMINA, N.A., red.izd-va; RYLINA,
'Tu.V.. tekhn.red.
[Micro-organtams in noils and higher plants] MikroorganismV pochvy
i vyeshie rasteniia. Moskva, Izd-vo Akad.nauk SSSR, lq_~S. 462 p.
(Micro-organisms) (MIRA 11-7)

KRASILINIKOV, N.A., SKYABIN, G.K., ARTAMONOVA, 0.1.,
A now nntiviral antibiotic violnrin, produced by Actinomycoa violaceug
(with summary 'in Buglish]. Antibiotiki. 3 no.3:16-22 MY-Je 158
(MIRA 11:7)
1. Inatitut mikrobiologii AN SSSR.
(AGTIKOKYGDSD
violaceus. prod. of antiviral antibiotic violarin (Rua))
(VIRUSM q effect of drugs on,
violarin, antibiotic prod. by Actinomyces violaceus
(Rua))
(ANTIBIOTICS,
violarin, antiviral properties & prod. by Actinomyces
vioinceus (Run))

KRASILINVOT, x.A.
Soil and climatic factors influencing the variAbility of micro-
organisms. Trudy Inst. mikrobiol. no-5tlO7-115 158 (miu 11:6)
1. Inatitut mikrobiologii AN SSSR.
(BACTERIA,
fiflabIlity. off. of soilA ollmate, roview't(RusU..
(SOIL* microbiolb&' : ~ ~ - " - -1 - I
bact, variability. review (Rua))

-AUTHOR: _Zrasilinikov, N. A.. Correoponding so,113o- 50-6-lo/45
Member AS MR
TITLE- Soviet "Gibberellin" (Sovetskiy 11,gibherellinll)
PERIODICAL: Vestnik Akademii nauk SSSR, Nr 6,
Pp. 70 - 73 (USSR)
ABSTRACT: During recent years, attention wan diverted towards the most
active microbes which stimulate Crowth. Amon;_st others, a
preparation was obtained from fungi of the fusarium-group,
which according to its properties, is closely related to the
gibberellin acid described in publications and which was designa-
ted as "GII-preparation, or respectively as Soviet Gibberellin~
Up till now, the socalled fungus Gibberella fujikuroi has been
knovn as producer of these preparations. This fungus has been
separated from the tissues of a sick rice-plant by the Japanese
scientist Kurozava for the first time in 1926. In 1939, another
Japanese scientist Yabuta, obtained an active substance in che-
mically pure form, which he called Gibberellin. At preoent, this
preparation is produced by many foreigm firrug. The effect of
Card 1/3 Gibberellin acid on the growth of plants was thorouChly investi-

Soviet "Gibberellin"
SOV/ 30- 58-6-10/45
gated in the Soviet Union by Professor M.Kh.Chaylakhyan who
used an American preparation. The Soviet preparation 11G" was ob-
tained from the fun,-us Fusarium sp., which is further described,
The preparation "Stadol" which was sent by Professor P.N.Brian
from England (Ref 1) wao also investigated for the purpose of
comparison. The separation and chemical purification. of the aub-
stance was carried out accordinz to the Stadol-method by the
collaborator of the Inatitute for Microbiology, AS USSR~ Yu.M.
Khokhlova (Ref 2). It is still unknown whether the Soviet prepa.-
ration represents Gibberollin acid, since special chemical in-
vestigatione are still required for this purpose. Like the
Stadol preparation, it acts already in quantities of I to 2 pg on
the growth of plants. The optimum concentration is 50 to 100 vg
per 1 liter of water. Great successes were achieved by wetting the
young plants (peas, cucuml)ers, main) which is also the case by
submitting the seed to a treatment with the "GII-preparation. This
preparation ought to be t;~ated by various Drovincial authorities
by expcrimental stations, as well as on kolkhoz fields in order
to clarify the possibilities of its use in the fields and gardens.
Above allf it is necessary to produce an adequate quantity of
Card 2/3 this preparation in the plants of the antibiotics industry

Soviet "Gibberellin"
SOY/3o-53-6-to/45
according to the proposed method and to guarantee it d1so in
the future.
1. Plants--Growth 2.. Growth*substances--Effectiveness
Card 3/3

26-56-7-16/48
AUTHO R i Krasil I nikov, N - A. , Corresponding Member -f the AS USSR
TITLE: Soviet Gibberellin (Sovetskiy gibberellin)
PERIODICAL: Priroda, 1958, Nr 7, pp 81-84 (USSR)
ABSTRACT: The author deals with gibberellin preparations developed
by the Laboratory of the Chair of Soil Biology of the Moscow
State University and compares them with foreign preparations
of the same type. Professor M-Kh. Chaylakhyan has made
thorough studies in the field, Preparation G (,Ipreparat G"'I
is based on Fusarium sp. fungus and has great influence on
the growth of plants (Figure 1). 'Preparat G" is sTill
being keDt in quotation marks, since it has not yet become
evident whether the preparation belongs to the true gib-
berelli;.j. Preparation D (preparat D) is based on soil
yeasts, is water soluble and looks like a brownish powder.
It also has great stimulating properties (Figure 2). Pre-
parations A, and A2 (preparat A,, preparat A2) have been
obtained from actinomycete cultures. They stimulate the
growth of peas, clover, sweet peas, 7ucumbers and grasses
(Figure 5),
Card 112 There are 3 photos and 6 Sovie'z; referen-~es.

Soviet Gibberellin
26-58-7-16/48
ASSOCIATION: Moskovskiy gosudarstvennyy universitet imeni M~V. Lomonosova
(The Mucov State University imeni M.V. Lomonoso-r)
1. Biology--USSR 2. GibbereLUn--Preparation
Card 2/2

SOV-26-58-10-19/42
AUTHOR: Krasillnikov, N.A., Member Correspondent i~S !jSSR
TITLE: The Absorption of aturally Radioactive Elements by Soil Mli-
croorganisms (Pogloshcheniye yestyestvenno-radioaktivnykh
elementov pochvennymi mikroorganizmami)
PERIODICAL: Priroda, 1958, Nr 9, PP 97-99 (USSIO
ABSTRACT: Naturally radioactive elements, such as radium, uranium, tho-
rium, etc., are distributed widely but thinly in nature. Ac-
cording to date by Baranov andiT seytlin (19411,oradium.amounts
in soils range 5from 2.96 105 to 1,94 . 40 , uranium_ 4
from 8.8 ~ 4 to 58.0 10 , and thorium from 2.6 , io
to 9.5 - 10 (Uranium and thorium in percentage expressions
of radium). Experiments have shown that small concentrations
,of natural radioactive substances are necessary for the in-
crease and stimulation of the life processes in the plant
cells, plant growth and harvest yields, There is also evi-
derice that the microorganisms of the soil utilize the radio-
active substances as energy sources for certain biochemical
processes. Azotobacter (chroococcum and vinelandii) absorb
the highest amounts within the briefest period of time, the
Card 112 root bacteria of luc.erne, pea and clover take up considerably

SOV-26-58-9-19142
The Absorption of Naturally Radioactive Elements by Soil Microorganisms
less, the least amount is taken up by spore-bearing bacteria,
micrococci or staphylococci. Among the actinomycetes, there
a--e strains which absorb high amounts of radioactive substan-
ces, about as much as ezotobacteria, while other strains only
take up litr1e. Thus soil. bacteria, in theit natural sLtes,
ab*orb and concent--i-ate radioactive substances occurring in
nature, pass them on to the plants, affecting them greatly.
the bacteria themselves also utilize these substances for
their own life prcce53eS. The experimental method of record-
ing the aboorption of radioactive substances by soil micro-
organisms is described in detail, There are 4 photos,
ASSOCIATION: Moskovskiy gosudar~itvonnyy universitet im. M,V., Lomonosova
(The Moscow State University imeni 11L.V. Lomonosov),.
1. Radioactive sobst=ces--Abscrption 2. Rzd1.oucti*,,e-
Radioactive
L
Card 2/2

SOV/25-'5-c-11-7/44
AUTHORt -Krasill-nikov, N.A., Corresponding Member of the USSR Academy
of'
TITLE% Living Treasures (Zhivyye klady)
PERIODICALs Nauka i zhizn', 1958, Nr 11, pp 14-16 (USSR)
ABSTRACT: The author reviews the stimulating properties of soil micro-
organisms upon the growth of vsrious agricultural plants. He
deals in detail on the stimulating effect of gibbereline,
first disdovered in 1926 by the Japanese scientist Kurozava.
It was found that of the 3 forms of gibbereline (Al, A2 and
A3), A, gave the strongest stimulating effect. However, as
pro mi a ng as the prospects for increasing the yields of agri-
cultural crops may appear by using these artificial stimulants,
much research work remains to be done for their practical
realization. There are 7 photos.
Card 1/1

KRASIj2,XDnZ,X,Av-
Antngonintic microbes nnd Rntibiotic substnneas no fnetors
Incrensing the resistAnce of plants to Infections [with summar7
in Hnglishl. Izv.AN SSSR Ser'.biol. 21 no.2:170-182 Mr-AD 158.
(MIRA 11:4)
1. Institut nikroblologli AN SSSR.
(PLANTS--DISEASE AND PEST RESISTANCE) (ANTIBIOTICS)
(SO ILS - -BACTER IOIOGY)

5 j' ~ -'Al / NO VAJ ~ .4 ,
IYERUSALIKSKIY, N.D., IMSHEMSKIY. A.A., KOSIKOV, K.V..KRASILINIKOV. N.A.
RAUTENSHPMN, Ya.I. I I ~ 11 1 i - 1-- . -
Matus Osharovich StreBhInskii; an obitun,7. Mikrobiologiin 27
no.2-271 Kr-Ap 456 (MIRA 11:5)
(STRESHINSKII, HATUS OSHAROVICH, 1912-1957)

0ASILtMIKOV, N. A.
"Antibiotic substances and their use in t~e br-ceding of p1anllsll.
rer)ort presentcd at a Joint ~3cssion of the Holo-7ical Dept. of A%' U56P and Biological
6 be
nn 14Edical Lepts. AN Gruiiya LSR, Tbilisi, 2E pt - 3 Oct 19~'-7. Vestnik Akad.
a
lqr~ 'Vol. 9f, lo. 1, pp. 121-12". (author lziriz~iqhvili, ". N.)
"auk bMt.

KTIASILI NIKOV, N. A.
"On the Part PLVed b1; Microorganism in Plant Nutrition.
report presented at the Congress of Biological Research In the Moldavian SM
16-21 Sept 1957, Moldavian Branch AS USSR organized togetlier with VASYMITIL.
Vestnik AN SSSR, 1958, V. 28, No. 1, p. 124-126 (author Kosenko, I. Ye.)

AUTHORS: _K_:~a~silln~ikov, ~N-~Corresponding SOV/20-120-4-59/67
Mem e __ , I Academy of Science5 USSR,
Korenyako, A. I., Artamonova, 0. 1.
TITLE: On Self-Suppression in Actinomycetee (0 samougnetenii u akti-
no4iaetov)
PERIODICAL: Doklady Akademii nauk SSSR, 1958, Vol. 120, Nr 4, PP. 900-903
(USSR)
ABSTRACT: In the study of the antagonism of Actinomycetes the authors
found a certain regularity in the particular nature of the inter-
specific interaction. As a rule the cultures of the same species
do not suppress each other. Antibiotics do not suppress their
own producer (Ref 5). This specific nature of antagonism served
ks a basis for the method of grouping and for the determination
of the species of Actinomycetes and for the differentiation of
the antibiotics produced by them. These methods permitted a
comparatively accurate separation without a failure for a number
of years. There are cases, however, where such a culture of
Actinomycetest when applied to the nutrient medium suppresses
the growth of its own cells and of the cells of races belonging
Card 1/3 to ito own species. No difforonooe as compared to the inter-

On Self-Suppression in Actinomycetes SOV/2o-12o-4-59/67
specific antagonism can be perceived. Zones of self-suppression
are formed (Fig 1), this phenomenon, however, being rare. It is
observed with the greatest frequency in pigmented species as
Act.violaceus, Act.coelicolor, Act. roseus, Act. viridichromo-
genes, but Tiso in not pigmented species, as Act. diastaticus,
Act.Rriseus. This phenomenon was studied. The investigations
showed, that this effect is caused by two factors: a) by phages,
which sometimes are the cause of self-suppression of growth,
or b) in other cases a particular substanceaausing the death
and the dissolution of cells. Pending final decisions, it was
called "necrohormone". Long-term research furnished the result
that marry Actinomycetes contain phages in a hidden state. These
are so-called lysogenic cultures. They are not dissolved under
normal conditions of growth. The phage appears only in a par-
ticular stage of the Actinomycetes.(Ref 9). Such lysogenic
Actinomycetes are sometimes uncovered by the application of
pellets of old culture on the recently sown patches of cells.
The zone free of growth forming around these pellets is caused
by phages, which become active.by an unknown manner (Fig 1b).
According to the experiments the authors drew the conclusion,
Card 2/3 that other factors than wtibiotics are to be made responsible

On Self-Suppression in Actinomycetes
ASSOCIATION:
SUBMITTED:
SOV/2o-120-4-59/67
here, that is to say a) actinophages, b) necrohormones. The ac-
tion of'the latter was proved for several Actinomycetes, as
Act.diastaticus, in some gray species, and in isolated races of
blue Actinomycetos and in other. Necrohormone substances were
found in races of Act.violaceus. They were isolated by physico-
chemical methods and were obtained as a red solution. They are
apparently a mixture of different chemical compounds. Necro-
hormones could not be obtained as yet in a pure state. There
are 3 figures and 9 references, 9 of which are Soviet.
Institut mikrobiologii Akademii nauk SSSR (Institute of Micro-
biology AS USSR)
March 6, 1957
1. Actinon;ycetales--Growth 2. Actinomycetales-Chemical analysis
3. ActinonWeetales--Physiology 4. Bacteriophages
Card 3/3

Qc
till
'Ou

a t i I I
o 1) ri n IV fr. 1, C.1, C. OJ? U C? n
1.11 ;~ ~ , 11 4 ,, ., t , fr
n r: t i jr. r c o t, 0 X
n -j o-- r 6 in,~nth-- u o or M. C 0. t",
i t Coulla Lnf!t L r m ;t';n "r~
I I m u bc I -a t ~i
h h c y v o r., t r r. n t c iia i o r. n a n m
m0nl
of t i r r- 1 1,ac r a
qf:,Ft ICO V , q.-. jj!j2 In- Q~-
t i an-i t I w
V e f." n. V U n u f~ t r e rid r-d i or
T, h ~,- ; 1 _4 o - ~ c~4
nilvnosu ho Ln,.?d.4 --;Ihovi d
gov.- :..nd re i r n c e ov
TT., I -

:-"0V/20-120-5-57/67
The Accumulation of Naturally fladioactive Elements by Scoil Licroorganism.,
1. Bacteria--Effects of radiation 2. Radioactive substances--Dosage determination
3. Radioactive substances--Absorption 4. Microbes--Autoradiography
Card 3/3

KRASILINIKOV. N.A.; CHATLARHYAN, M.Kh.; SKRYABIN, G.K.; KMYIWVA, Tu.M.;
KONSTANTIROVA, T.H.
Stimulating effect of gibberellins of different origin. Dokl. All SSSR
121 no.4:755-758 Ag 1.08. (MIRA 11-.9) 4
l.Moskovokiy gosudarstvonny7 ur)iversitet im. M.V. Lomonosova Institut
fiziologii raoteniv im. K.A. Timiryazeva AN SSSR i Instittit mikrobiologii
AN SSSR. 2.Chlon-korr6spondent AN SSSR (for Krasillnikov).
(Gibberallins)

17(2) BOV/20-123-2-44/ 50
AUTHORS: __Kxzai1!n1kux., N. A.,_ Corresponding Member, Academy of Sciences,
USSR, Zvyagintsev, D. G.
TITLE: The Application of Fluorescent Microscopy in Incident Light to
Soil Microflora Investigations (Primeneniye fluorestsentnoy
mikroskopii v otrazhennom avete dlya izucheniya pochvennoy
mikroflory)
PERIODICAL: Doklady Akademii nauk SSSR, 19J8, Vol 123, Nr 2, PP 366-367
(USSR)
ABSTRACT: The authors suggest a modification of the method of direct
microscopic investigation of the soil microflora: instead of
observing a soil-suBpension in permeating blue light an un-
destroyed section of fresh soil is lighted by the mercury-
quartz lamps SVDSh 250-2 and -3, and studied in the incident
light. Thus, it was possible to see the natural normal dis-
tribution of microbes in the soil as well as their colonies on
soil particles of any size. The staining method remained un-
changed (Refs 1,2): fluoro chrome acridine orange. The color
microphotographs (Pigs 1-3) give an idea of the louation of the
Card 1/3 microbe in the soil and of its main forms. It was possible to

SOV/20-123-2-44/50
The Application of Fluorescent JMicroscopy in Incident Light to Soil Micro-
flora Investigations
find out that the soil microorganisms are placed in their nat-
ural state on the surface of soil particles. Only a few of them
are floating freely in between. Some particles are densely
(Fig 1 a), others, however, only weakly populated (Fig 1 b).
They form colonies different in size (4-20 up to 100 and more
individual microbes). Single cells, and chains occur relatively
often. The bulk of the bacterial cells in the soil is in a
state of ball- or coccus-like fozms; smaller or bigger rods
are rare. Mycelial hyphae of actinomycetes and fungi occur.
Organic materials, such as peptone, saccharose, starch, and
mannitol caused an impetuous development of microorganisms in
humus. A lot of freely floating cells occurred. The authors
successfully applied the method suggested for investigating the
adsorption by the soil of individual species of bacteria (Fig
3). Some species (Pseudomona pyocyanea) are adsorbed in great
quantities, some others in a smaller degree (Bacterium coli,
B. mycoides), and a third group not at all. As fluorescence
device 01-17 was used. There are 3 figures and 2 references,
Card 2/3 1 of which is Soviet.

SOV/20-123-2-44/50
The Application of Fluorescent Microscopy in Incident Light to Soil Micro-
flora Investigations
ASSOCIATION: Moskovskiy gosudarstvennyy univer5itet im. M. V. Lomonosova
(Moscow State University imeni M. V. Lomonosov)
SUBMITTED: June 27, 1958
Card 3/3

170), 17(4)
AUTHORS: Krusilfnikov 11 A., Corre3 ending Member, sov/2o-123-6-45/50
P
Academy of Sciences, USSR, Chaylakhyan, M. Kh.jAseyevat I. V.,
Xhlopenkova, L. P.
TITLE: On a Gibberella-Like Substance Formed by Soil Yeasts (0
gibberellinopodobnom veshchestve, obrazuyemom pochvennymi
drozhzhami)
PERIODICAL: Doklady Akademii nauk SSSR, 1953, Vol 123, lir 6,
pp 1124 - 1127 (USSR)
A!~STRACT: The authors point out the otimulating effect exerted by the
gibberella. preparation Ur 1 which had been isolated by them,
and by the pure gibberella A 3 on the 1'r1j,ovith of Rudbeckia
bicolor (Ref 2). Physical-chemical properties and chromate-
grams characterized the mentioned preparation Nr 1 as
gibberella A3 or some compound related to it. The preparation
investigated -in the present paper comes from Torula pulcherrima,
a yeast fungus that is especially prevalent in turf-bleaching
earths. It grows well in media without nitrogpn with and
Card 1/4 without addition of a6ar. On a,,,ar this yeast fungus forms

On a Gibberella-Like Substance Formed by Soil Yeasts SOV/2o-123-6-4/50
mucous, vaulted, semitransparent or transparent colonies
deliquescing on the surface of the cult-are medium. Externally,
they are very much like the colonies of Azotobacter chroo-
coccum. Torula has very large (10-154 and even more) regularly
globular cells (Fig 1). They propagate by gemmation, without
spores. For their multiplication the liquid synthetic medium
of Chapek was used. There, they grow best. After the medium
has become turbid (15 - 20 days), the active substance is ob-
tained as a powdery raw prodi~ct by advorption on charcoal
and elution with organic solvents. The pre;~aration obtained
proved to be highly active and was tested in comparison with
gibberella preparation ITr 1 as well as with chemically pure
gil)I~eralla A3on rosette-like plants of Hudbeckia Wcolor.
The preparation in the form of a 0.02r'o' aqueous solution
(content of active substance in one drop about 100 was intro-
duced dropwige into the center of the ronette or into the
axil of an upper leaf of the plants. The controls developed
water drops. Figures 2 and 3 as viell as table 1 show that
the physiological activity of -ibberella A (Fig 2:1) in
3 0 -
Card 2/4 equal to that of the preparation IIr I (Fig 2:2). The sample

On a Gibberella-Like Substance Formed by Soil Yeasts SOV/2o-123-6-45/50
from Torula is but little inferior as to the growing of tl:e
stems by 5-6 days, as to the fcr~nation of flower buds and
the bursting by 9-10 days. The plants on the Torula pre-
paration (Fig 2:3, 3:1) are of more compact structure, since
the stem is abundantly foliaged, the leaves are of a deeper
green, the internodes are ohorter whereas the lateral shoots
grow inore regularly and are not so elongated. The controls
remained always in the rosette stage (Pip, 2:4, 3:2). This
proves that gibberellasand their related substances are meta-
bolites which are not specific for t:ic Fusarium ftingi alone,
but are characteristic also of other microorganisms, in
partictilar of soil-yeasta. Thure are 3 fi,~urcs, 1 table and
3 Soviet rei7e--ences.
ASSOCIATION; Moskovskiy gosudaratvennyy universitet im. M. V. Lomonosova
(Moscow State University imeni M. V. Lomonosov) Institut fizio-
logii rasteniy im. K. A. Timiryazeva- i Institut mikrobiologii
Akademii nauk S3SR (Institute of Piant Physiology imeni K. A.
Timiryazev and Institute of Microbiolotgy of the Academy of
Sciences, USSR)
Card 3/4

PWROSTAN, Azniv Petrosovna; UASILINIKOV, N.A., prof., red&;
KUMIGTAH, I., tekhn.red.
(Ecological characteristico of the nodule bacteria in the
Armanian S.S.R.] Mkologichaskie osobennosti klubenlkovykh
bakterii Amisnakoi M. Pod red. N.A.Krasillnikova. Erevan,
Izd-vo PI-va selt.khos..Armianskoi SSR, 1959. 280 p.
(MIAA 13zll)
1. Chlen-korrespondent AN SSSR (for Krasillnikov).
(Armenia--Micro-organiame. Nitrogen-fixing)

MS~IKIKOV. N*A.
Factors transforming the properties of micro-organisms. Izv. AN
SSSR. 3~r.blol. 24 uo.6:814-831 N-D 159. (MIRA 13:4)
1. Institute of Kicrobiology, Acadeaq of Sciences of the U.S.S.R.,
Moscov.
(KICRO-ORGANISKS) (VARIATION (BIOLOGT))

KRASILINIKOV, N.A.; KOTEUV, V.V.
Adsorption of phosphatases of soil micro-organisms by conr roots.
Mikrobiologila 28 no.4-548-550 JI-A,- 159. (MIRA lz:lz)
1. Pochvennyy institut Holdavskogo filiala AN SSSR.
(PHOSPHATASS) (CORN (MAIZM)) ROOTS (BOTNAY))

KRASILINIKOT, N.A,
"Marine (deep-sea) microbiology- by A.B. Kriso. Reviewed by N,A~
Krasillnikar. Mikroblologila 28 no.6t944-948 N-D 159.
(SU WATIR--BACTIRIOLOGY) NIILA 13:4)

17(2) SOY/20-128-4-56/65
AUTHORS: Kras i1 In ikoy.t . *.-Aii'-"Corre a pond ing Member, IS USSR,
akrr9b'Vfi_, G. K., Aseyeva, 1. V., Korsunskaya, L. 0.
TITLE: Deh'ydrogenation in the 1,2 Position ol"' Hydrocortisone by
Means of Myoobacterium op. Nr 195
PERIODICAL: Doklady Akademil nauk SSSR, 1959, Vol 128, Nr 4, pp 836-839
(USSR)
ABSTRACT: During the last years it has become possible to make use
of microbiological processes for the production of hormones
occurring in organisms: suprarenal gland, reproductive
hormones, and their derivatives. New microbiological proces-
see were developed for the production of cortisone (substance E)f
hydrocorti8one (substance F) and their derivatives# on the
basis of hydroVlation of progresterone into II 0-oxy-progres-
terone by microorganisms (Ref 2). Highly effective hormonesp
namely prednisone ( AE) and prednisolone ( AF) were industrial-
ly obtained in good yields by means of Corynebac.terium simplex.
They are used for inflammations (Schering, USA, Ref 3). This
method proved to be more simple and less expensive than
chemical processes. Aotinomyces lavendulae, bacterium cyclo-
Card 1/3 oxydans et al, during fermentation develop a mixture of dif-

SOV/20-128-4-56/65
Dehydrogenation in the 192 Position of Hydrocortisone by Means of Myco-
bacterium op. Nr 193
ferent ater&ids. An industrial production of A E and AF is
difficulig due to the necessary separation of this mixture.
The authors made investigations in order to find highly active
microorganisms which are able to transform biologically
hydrocortisone (1) and prodnisolone (II). The most productive
cultures were looked for in vegetable materials, decomposition
products of the aoil, in the oral cavity of man and animals,
and in other natural, nutrient media, and numerous strains
of Actinomycetea, fungi and bacteria were isolated. 10-15 mg
of the initial steroid chemically producedo nere added to
2 ml of 8VIo ethanol. The transformation of steroids was con-
trolled by decreasing distribution chromatography (Ref 6).
By means of this method cultures vere obtained which are
able to traneform the initial substances into cortisone,
hydrocortisone at al. The culture mentioned in the title
actively caused the mentioned process and produced predni-
aolone and prednisone. "B" with 1% of yeast autolyeate, 1%
of glucose in distilled water proved to be the optimum medium
for highest prednisolone yields (790/'o). After 5 hours the
process is finished. If fermenta-tion is continued, prednisolone
Card 2/3 decomposea. Figuro 1 shows chromatograms of the transformation

SOV/20-128-4-56/65
Dehydrogenation in the 1j2 Position of Hydrocorlisone by Means of Myco-
bacterium sp. Nr 193
process. The quantitative yield was spectrophotometrically
determined besides the i4entifioution of the final produots.
They were chemically isolated. Yu. N. Chirgadze (Inatitut
biofiziki All SSSR - Institute of Biophysics of the AS USSR)
conducied the identification by means of infrared spectra
(Fig 2). There are 2 figures and 6 references.
ASSOCIATION: In5titut mikrobiologii Lkademii nauk SSSR
(Institute of Microbiology of the Academy of Sciences# USSR).
Moskovskiy gosudarsivenrxyy univereitet im. M. V. Lomonosova
(Moscow state UniYersity imeni M. V. Lomonosov)
SUBMITTED: July 10, 1959
Card 3/3

4-
KOMARVITSKU,, N.A.,, prof.; TOMIN,. M.P.p all4emik;_XHASILINIKOV. N.A..
i - a--
prof.; KURSAIIOV,, L.I., prof.' :red.; TSESHIIISKAYA,, Ij.I.? ~e
PARSADPWOVA, K.G., red. izd-va; PAVLOVAv V.A., tekhn. red.
[Classification key of lower plants in fivIe volume) Opredelitelt
ni.ishikh r4vtenii v piati tomakh. Moskva, Gos, izd-vo "Vysshaia
shkola.0 Vol-5. [Lichensp bacteria, and actinorqcetes] Lishainikip
bakterii i aktinomitsety. Pod obshchei red. L.I.Kureanova. 1960,
290 p. (MIRA 14:9)
(Lichens) (Bacteria) (Actinomyces)

MASILNIKOVp N* A.
"On The Significance Of Microorganisms In Soil Toxicosis".
report submitted for the 7th Congress of International Society of Soil Science
Madison, Wisconsin, 15-23 Aug 6o.
)

MWILMJW, N. A. and GUrJMAN, G. B.
"The Microflorn of the Siberian SiDworm Dendrolimus Sibiricus."
report presented at the Intl. Congress of Entomology,
Vienna) Austria, 17-25 Aug 1960.

Tireless explorer of the sea depths. Priroda no.60-6
is 16o. (XIu 13:6)
1. Chlen-korreepondent Alcadenii nauk SSSR.
(Iries, Amtolli Eveeevich)

DU I L I NI NOV...#
Prinpipleo underlying the claosifioation of aotinmyogtoo.
Trudy Tnot. miarobiol. no,,8&7-20 16n. (MIRA 2481)
(ACTINOMYCETALES)

N.A.
Im -olusifiost Am ok'rotinompet" produoing autibiotio
&dos 67r
substawW. Tra4 Inst. miarobiol. no.8t2l-28 .160. (MIVA 1411)
(ACTIHMMULES)

KAIAKUTSKIY9 L.V4,-)qWILINIKGVq N.A.
'I . ~ ...;
F6rmation of sclerotia, by actinomycetes and systemic position
ot the genus Chidflia. Trudy Inat. microbiol. n0-8--45-55 160.
(ACTINC14YCETALF,S) (MIRA 14: 1)

KRLSILINIKOVy N.A.; KoRimmog Aal.; NIKITIM, N-1.
Aotinomyr-en j~~porusv a subgroup of aotinomycetes of the' globis-
porus group. Trudy In3t,. microblol. no.895&-85 160. (MIRA 14:1)
1, InoUtut mikrobiologii AN*SWR.
(AGTIN%ffCETALFS)

NIKITINAp N.I.; KORENYAKO,
Cultureo of the specieo Actinomyces otreptomicini Kraos, Trad
Inst. microbiol. no.W5~-.103 160. (MIRk 1-4- Vif
1, Institut mikrobiologii AN SSSR.
(ACTINCMYGETALES)

NIKITINAf N.I.; KORENYAK09 A.I.; P" LIIIIKOV9, ~.A. -_
Actinamycea-Ifulgariso Trudy Inst. microbioi. no.8:104-115 160.
% (MIU 14:1)
1. Institut mikrobiologii AN SSSR.
N
(ACTINOMYCIVAIMi

KORmU*OtA.I,4,ILINIKOVj N.A.; NIKITINA, N.I.
Aotinomycee levoria. Trudy Inat. miarobiol, no.8s.U6-j3R 160.
1 (HIRA 14t1)
1. Inotitut mikrobio3~ogii AN WSR*
(ACTINWMALES)

KORENUKOv Aj.;_.,~W KITINAp N.I.; SOKOL(YVA,, A.I.
Actinomycates of the fluorescent group. Trudy Inst. microbiol.
no.Ss133-159 160. (MIRA 14:1)
1. Institut mikrobiologii.AN 4SSR.
(ACTIN(WOETALES1

KRASILINIKOV,, N.A.; NIKITIM# N.I.j KONDRATIUVA9 I.K.
Aotinomyces pne=oniouo no spot a now species of the globispor"O
groups Tru4y Inst. microbi6l. no.W60-169 160. (MIRA -14:1)
%-,~ N
(ACTINOKYCBTALES~

KWILINIKOV, N,A,.; VINOGRADOVA, ~.A.
Aotinomycetes of the chromogenes group. Trudy Inst. microbiol.
no.8:202-225 160, (MIRA-149i)
1. Moskovakiy gosudarstvennyy universitet,
(ACTINOMYCEULE~)

KUCHAIEVAp A.G.; KFASILINIKOVI, N.A.; SKRYABINq G.K.; TAFTYKOVA, S.D.
. ll~-
Actinomycetes of the olivoohmmogenes. Trudy Inat. microbiol,
no.gt226-253 160. (MIU 14's 1)
~ACTPICMYGMIM)

KRASILINIKOV, N.A.; AGREp N.S.
WAZWAUUUV~
Aotinomycetes of tbe eyanoalbus group. Trudy Inst. microbiol.
no.81254,-274 160. - (MIRA 101)
1. Moskovskiy gosudar8tvennyy universitet.
(ACTINOMYGETALES)

ARTAMONOVA, O.I.;,KRAS TIA.
Actinomyceteo of the violaceus group. Trudy Inst, microbiol.
no.8t275-337 160a (KLU .14: 1)
(ACTINOMYGETALES)

,UMI.LINIKOV., F1,bA.;-SKRYAB1Nj G,K.
Investiption of the field of antibiotics and the antibiotAge..
industry in Japan. Antiblotiki 5 no.'3tl2l-125 My-Je(160"
(JAPAN-ANTIBIOTICS) MIRA 14:6)

CHLYLAKHTA-N, M. Kho; KRASILINIXOT,_ N.A.; IUCHAYETA, A.G.; ITANOV, I.I.;
KWPMOVA, L.P.; ASIMA. I.T.; DAVCBMO. B.L
Gibberellin production and the deteirmination of its physio-
logical activity in connection with Its use in plant cultivation.
Fislol.rasto 7 noel!UZ-120 160a (HIPA 13:5)
1. X.As Timiriazey Institute of Plant Physiology and
Kicrobiolog7 Institute of U.S.S.R. Academy of Sciences, Do-
partment of Soil Bioloar of Moscow State University, Moscow,
and lurgan Plant of Medicine Preparations, Kurgan.
(Gibberellin)

KRASILINIKOV9 N.A.; ZHUKGVAq R.A.; YASHISHp V.B.
amwazav
Possibility of twing antibiotics to protect the outer fibrous sheaths
of underground power cables from destruction by micro-organismse
Mikrobiblogiia 29 no.3;446-450 My-Je 160. (MIR& 13-7)
1. Institut mikrobiologii AN SSSR.
(ANTIBIOTICS) (EIMTRIC CABLES-MAINTEWCE AND REPAIR)
(BACVRIAt CELLULOSE-DEOMPOSING)

KRASILINIKOV, N.Ai.,--.-YUAN' TSZI-SHEN (Yuan Chi-she^ng]
A new apecies in the group of Actinomyces aurantiacus.
Mikrobiologiia 29 no. 4.482-489 JI-Ag 160. (MIRA 13:10)
lo Institut mikrobiologii AN SSSR.
(PEIPING REGION-ACTINOMYCES)

BEMCIVAq Ye.F.; IZRAI4'SKIY, V.P.; WHENETSKIY, A.A.; EROILINIKOV N A -
" --f-05
KISHUSTIN, Ye.N.; NAUMOVA, A.N.; RAUTENSHTEMTv Ya.I.
E.V.Runov; obituary. Mikrobiologiia 29 no.6:945-94~,N-D 160.
(MIRA 11,: 1)
(RUNOV, EM VASILIEVICIlt 1901-1960)

KRASILINIKOV, N.A.; TEGOROVA, S.A.
Restoration of pigmentation and antibiotic properties in laucomutants
of Actinomyaes coelicolor by use of microbial metabolites. Dokl.
AN SUR 134 no-5:12,18-1221 "' 160. (MIRA 13: 10)
1. Chlen-korrespondent AN SSSR (for Krasillnikov).
(ACTINOMYCES) (VARIATION (BIOLOGY)) (BIOTIN)

-) T I ~ t,l
. -