SCIENTIFIC ABSTRACT SPIRIN, A.S. - SPIRIN, K.F.

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• Collection: Scientific Abstracts
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• Sequence Number: 16
• Publication Date: December 31, 1967
• Content Type: SCIENTIFIC ABSTRACT

USSR/Microbiology. General Microbiology-. System- F-1
atics,, .7.1orphology, Cytology.
Abs Jour : Ref Zhur - Biol., No 14, 1958, Yo 62199
-Author : -.SDirin L'L-.S 1clo-.crGkiy A.N., Shugayeva N.V0,
Vanyus'hin B.
Inst 4.-
Title : Studies of tno Specificity of the Species of
Nucleic Acids in Bacteria. .
Orig Pub : Biokhimiya, 1957, 22, No 4, 744-754
Abatract : The Mil'A and DNA nucleotide composition was studied
in 19 different succles of bacteria and acti-
aoziycetos. The nuclootide composition of D-NA
uncovers a distinct speelf1city olo species. being
very sirailar in closely related species, and dif-
ferinog shawply In diztant spocies. In this,
Protcus vuloaris and Aorobactor aoroso Ines differ
considerably, accordla3 to t.aa DNA compouition,
frwm other spocies. Entorobacteriaceae force
Card 113

AUTHOR. SPIRIN,A.S., BELOZERSKIY,A.~:. PA -- 316q
TITLE- -Z-6~ratiive Studies on the RAIhoauo]--3:1~ A-,-.I.d Composition in the
Different Species of Bact~rn.~L. (Ss-a:!rr-.Lt~q'L'nqye izucheniye aostava
ribonukleinovylkh kislot u razlicEnq!;~.~. -1.iov bakteriy, Russian)
FERIODICAL: Doklady Akademii NaWk SSSR, 195',?$, Vo" -11.3, Nr 3, PP 650-651
(U.S.S.R.)
ABSTRACT: The present paper investigates the EWA (Ribonuoleic acid) in different
types of bacteria in order to find out to what extent the co=osition
of RNA changes in dependence of -the inherent, nature of the organi,9m,
and to wbat extent it is in corTelation with the composition of IRA
(de2co;yribonucleio acid). The same methods of hydrolysis for chroma-
tographv were employea as in the pree9ding work ('Biochamistrys 1956t
21, 768). In order, however, to increase accuracy all foar- mono-
rraoleotides were distributed over the on-Aimensional descending ah-rem-
togram. This ms done by the application of two zolvumtq whi0h Warre
allowea to pass in one and the same direction. Resulta showed th-at in
the case of the bacteria investigataa (staphylococous pyogenes auxeuz,
pasteur-,lla tularensis, brucellaL abort-as, proteus morganii,
Esscherichia coli, salmonella typhosa, shigella. 4ysenteriae, coryne:-
bacterium diphtheriae, pseudimonas aeruginosa, saroina lutea, Myco-
bacterium tuberculosis BCG, Actinamyces globisporus streptom) the
Card 1/2

PA -- 3-169
ComDarative Stidies on the Ribomicleic Aoid Compoaition in the
DiFferent Species of Bacteria.
omposition of DNA differed widely. On the other hand, the nucaec-
tide composition of DNA is very mimilar even in the caae of widely
differing types. Thus it may be concluded that with respect to IINA.'
even in the case of an investigation of summmary ocmposition, there
are numerous stages, which, however, cannot be said with respect to
IOTA. (1 Table, I Slavic Reference).
ASSOCIATION: Institute for Biochemistry "A.N.BAKE-1 and Faaulty of Soil Biology
of Moscow State Univex%ity w.vj~=Nosor,.
PRESENTED BY: OPARIN,A.I., Member of the Academy, on 15.1-1957
SUBMITTED: 11.1.1957
AVAILABLE: Library of Congrezz
Card 2/42

SPIRIN, A. S. (Dr.)
"The RNA Composition of Certain Bacteria and Its Correlation with DNA
Composite Chemistry."
report presented at the 4th International Congress of Biochemistry, Sep 1958, Vienna.

O"PIR-M, !,k. s.
A.S. Spirin and L. P. Gavrilova
"The results of investigations of ribonucleic acid of the tobacco mosaic virus."
report presented at the 10th All-Union Cont. on HIghly bk)-lecular CowpouMU,
Biologically Ar-tive Polymer Compounds, bbscow, 11-13 Jun 1958- (Vefit- Ak
Nauk WSR, 1958, No- 9, pp- U.1-113)

YK;ICA ~ec 4 Vol 12/2 Yed. Micro. Feb 59
405. CHANGES IN THE NUCLEIC ACID CO.MPOSITION AS A RESULT OF
EXPERIMENTAL HEREDITARY TRANSFORMATIONS OF INTESTINAL
BACTERIA (Russian text) - Spirin A. S. . Belozersky A. N. .
Kudlay D. G. . Skavro-n-;' 7aya ~. and Slite re~a V. G. Inst,
of Biochem. , Acad. of Scis of the USSR, Moscow - BTOKHI%IIYA 1958. 23 1
(154-163) Tables 4 Illus. I
Some intestinal bacteria and their unusual forms were studied. These forms re-
sult irom hereditary changes caused by unfavourable influences. suchas distilled
water. cultivation without transfer, antibiotics. These forms can be divided into
2 groups. distinguished by their properties and by the composition of their nucleic
acids. Changes of the DNA composition correlate with transformation of antigenic
structure. biochemical properties and susceptibility to antibiotics. The RNA com-
P --ition. la contrast to DNA. is not changed.

SPIRIF, A.S.
Spectrophotometric determination.of the total amount of nucleic
acids [with summary in English]. Biokhimiia 23 no-5:656-662
S-0 158 (MIRA 11:11)
1, Institut biokhimii iment A.No Bakha Akademii nauk SSSRI Noskvao*
(NUCLEIC ACIDS, determ.
spectrophotometric: determ. of total acids (Rue))

-,SPIERIN, A.S., SKAVRONSKAYA, A.G., PRETELI-MARTIMS, A.
Bucleic acid content of Escherichia coli during the aging of the
culture [with summary in Englishle' Mikrobiologiia 27 no*3:273-275
YT-Je '58 (MIRA 11:9)
L Institut biokhimii im* A.W. Bakha AN SSSR i Institut mikrobiologii
i epidemiologii im. N.Y. Lmaleya AMPT SSSR&'
(NUOMIC ACIDS. metab.
E. Coli, eff. of aging of culture (Rus))
(ESMRICHIC COLI, metab;
nucleic acids, eff. of aging of culture (Rus))

A W! F; x ~i'.-3; Bv. K. , Shugayeva, N. V.,
1914-11ft, IL. S.
F, The Jje~-~jcxyrfbonuc lei e Acid ComposVion of D-ifferdn"
Species of Actinomycetes Wstak"Rtei~okF.
k1slot u razlichnykh vidoy aktinqjifllsetov)
P'EIRTODICAL. ;~oklady Akademii Nauk SSSR, '1958, Vj", !'.9, Nr' R',
PF, 330-3.32 (USSR)
ABSTRACT: Tne study:of the nUOj*Otijq CoMpogitjon of thr-se
(DNA) in other organism, espeoially in ioaetkr,',&, Led
the solution of several problems of the pe~,uliarilly
cof species of these compounds (refs 1-4). How;~ver, tho
mi,.s .-C ths r3pecifity are not yet determi.iie-i. It. ia rii;t~
~e-t clear it, ncrw ar the compoulLion of DNA dil'i"ers Jit
W
biologically a.
nA systematically related rjyper (-z~,fs 7,A).
In Lhe present work the compos.1tion of
acid -is studied within a rather timited grr~;p
VI-ga(LiSMS, as mentioned in the ti~;i~. Inc,
of' the t
pecalarity of Npeolas of DNA, ttv, or

E: ro.'. o v ; d orPA1=8
-~!rjc;es in the. systematics of thi rr,
a d 1 gi_-r- be help. The me thods of Lnvess ti St; .-;r, w o

The Pesoxyribori2cleic Pcid ComposItior of J)Iffererit.
Species of Actinomycetes
kre!z
ce.mp.-vil-Icit of I)NA are g-livi-ri frz. 6 Jlypt--,~ n. e
as well as fov Mycobacterium tul ,rlr.-r!u ~4 s L: k
for M.diphtheriae. These data are fh~: M4~laaq -;-t, Z.-
The value of r b a r i p I i c f f 1'.
15 The vn-'y prImpry ind-?x .f !i.f
I"i y ~I, DNA Wril ca up al V;:tas I r
4n th;~ of ylirrcgen bat?-.z~ I e t h C-
Irk thin .,,f a] 1
iiar ~;he r..: f~i,t r) i t.h~ ri
-Y P~ :. ', , - " rc- f 4) 1 n th- s --n.
Ts A 6
rhe iui--t ~-,ctriam4~ position among ,he typ i
a , s , rc- ng d I f f P r,:: n r - -b e i DN A ~- api po
!h,
rl~~rfj ''rial: ~Iie fi-,sr. and th~-, 1, tactp,-,%

Acid Composition of Different 20-119-2-39/60
of Actinomycetes
repre$ent two different rather isolated groups of the
species of the-fetinomyostes (class Actinonyootales
according to Vaksman), Roweverp 'he relatively high
value of the relation (4 + To) 0. (A + T) as compared to
other bacteria agrees with the fact that these micro
bacteria belong to the same systematic category as the
actinonyostes (refs 6t7)- On the other hando ,he comparison
of the MA of the Actinomyeetes and the X.tuberculosis with
the I)NA of N.diphtherias leads to-tho conalusion thn't "he
latter-type does not belong to the class of the Actinomycetes
and even less to the species of the Mycobactorium. It.must
be placed to an inde5 endent family of the order .
Eubacteriales (ref 7 - In the individual types of
Actinosycates the DNA composition to clos.oly related so that
they form a group which in perhaps systematically close.
Statistically the 4 types of Actiftasycetes can absolutely
not be distinguished. Thus,.no, characteristic types exist
in the ease 9f types of one and the an* species as to the
DNA composition. The authors gave the explanation earlier
Card 3/4 (ref 4): The mentioned differences ooncern only single

n0-1 19-2-314, 6C.
'The Desoxyribonucleia Acid Composition of Different &
Species of Actinomyootes
molecules or even their sections while the main mass
remains identical. The diffeten-c-en are too slight to be
deiected by the applied mothodR. As for as the two othef
species of Act*inomycetts (Proactinomyces and Micromonospora)
are concerned, a difference from the Actinoicycetes can be
observed in'sipite of the clone reIntion as to the DNA
composition-."However,'It in too sm&ll to draw final
concl usions from it.
There ar.- I table and 7 referenceep 3 of which are Soviet.
kSSOCIOTON: Moskovokiy goiudarstvennyy univerbitet im. V. V. Loincnos,:vtt
(Voscow State University imeni H. V. Lomonosov)
fnatitut biokhimii im. A. N. Bakha Akademii,,nauk SSSR
~Institute for Biochemistry im*ni A, N. BakI As USSR)
PRESPHTED: November 21, 1957, by A. 1. Oparin, Member, Academy of
.Sciences,AS USSR
Card 4/4

SPIRIN, A. S., and BELOZMSXIY, A. N.
"A Correlation between the Compositions of Deoxyribonucleic and
Ribonucleic Acids." Nature, Vol. 14, no. 4628, p. 111, 1958.
Inst. of Biochemistry im A. N. Bakh Acad. Sci. USSR, Moscow.

GAVRILOVA, L.P.; SPIRO. A.S.
Infective r,ib--o-nuc!Ce-i'c'--Aicid of the tobacco mosaic virus and its
behavior during the lose of Infectivity. Biokhimiia. 24 no-3:
503-513 M.Y-Je '59. (MIRA 12:9)
1. Institute of Biochemistry, Academy of Sciences of the
U.S.S.R., and the Faculty of Biology and Soil Science, the
State University, Moscow.
(VIRUSES, metab.
tobacco mosaic virus. ribonucleic acid, role in
virulence (Rue))
(RnORMLICIC ACID, metab.
tobacco mosaic virus, role in virulance (Hus))

SPIRIN, A.S.- GAVRILOVA, L.P.; BELOZFaSKIY, A.N.
Hature and methods of quantitative estimation of the "hyper-
chronic offect" of nucleic acids. Biokhimila 24 no.4:6oG-611
Jl-Ag 059. (MIRA 12:11)
1. Institut biokhtmii in. A.N.Bakha Akademil nayk SSSR I
biologo-pochvenny-r fakliltet Gosudnrstvennogo universiteta
in. M.V.Lomonosova, Moskva.
(NUCLIBIC ACIDS chem.)

SPIRITT, A.S.; GAVRILOVA, L.P.,- BRESLER, S.Ye.; MOSEVITSKIY, M.I.
Studying the macromolecular structure on infectious ribonuclelc acid
from tobacco mosaic virus. Biokhimiia 24 no-5:938-947 S-0 '59.
1 (MIRA 13:2)
1. Inetitut biokhimli im. A.N..Bakha Akademii nauk SSSR, Moskva, i
Institut vysokomolekalyarnykh s6yedinenly Akademii nauk SSSR, Lenin-
grad.
(VIRUSES chem.)
(RID017UCLEIC ACID metab.)

170) SOV20-124-4-59/67
AUTHORS: Gavrilova, L, P., Spirin, A. S., Belozerskiy, A. N., Corresponding
Member AS USSR
TITLE: Spectrophotometric Study of the Effects of pH and Ion Strength on
the Stability of High Polymer Ribonucleic Acid in Solution
(Spektrofotometricheskoye izucheniye vliyaniya pH i ionnoy sily na
stabillnost' vysokopolimernoy ribontikleinovoy kisloty v rastvore)
PERIODICAL: Doklady Akademii nauk SSSR, 1959, Vol 124, Nr 4, PP 933-936 (USSR)
ABSTRACT: Ribonucleic Acid (RNA) in vitro is extremely unstable. Even its
simple preparative production from cell organisms results in a
higher or lesser degree of degradation (Ref 1). Nor do any of the
subsequently suggested production methods (Refs 2-7) furnish any
evidence for the fact that the spontaneous losses in infective power
occurring in this process (in this particular case the infective
power of RNA from the tobacco mosaic virus - TMV) or a direct de-
gradation of the polynucleotide are conditioned by the chemical
instability of the polymer concerned, subject to certain conditions.
On the one hand ribonuclease traces may occur in RNA preparations
and cause the fermentative degradation of RNA in the course of in-
cubation. On the other hand it is not known whether the spontaneous
Card 1/4 loss in infective power of virus RNA is caused by the one or the

SOY/20-124-4-59/67
Spectrophotometric Study of the Effects of pH and Ion Strength on the Stability
of Higher Polymer RibonucleiQ Acid in Solution
other type of degradation or whether it is governed by entirely
different mechanisms. In order to find out whether a progressive
spontaneous degradation. of polynucleotides takes place in the
solutions of high molecular BNA, the authors employed spectro-
photometric criteria. After all, the decomposition of the nucleic
acids is accompanied by an intensification of their ultraviolet
absorption (the "hyperchromic effect", Ref 8). In the case of the
instability of the inter-nucleotide bonds of the polynucleotide,
the ultraviolet absorption must increase progressively. High
molecular RNA and TMV were used as materials. They were obtained
by means of several re-precipitations ~vf the leaf juice of mosaic-
diseased tomato plants wi-th ammonium sulfate, and by means of
threefold preparative ultra-centrifuging. This procedure ensured
the high purity of the TMV preparations. including, it seems, also
that of ribonuclease admixtures. The RNi obtained therefrom possess-
ed biological activity and infective power (about 1 % of a virus
quantity of equal weight), immediately after production. It did not
contain any virus particles. It thus constituted a native RNA pre-
paration. For experiments with long incubation periods, buffer
Card 214 solutions with ion strengths ~b) of 0.01 to about 1 and different

SOV/20-124-4-59/67
Spectrophotometric Study of the Effects of PH and Ion Strength on the Stability
of Higher Polymer Ribonucleic Acid in Solution
PH were used. The RNA content of the solution was about 20-25n/ml.
Incubation takes place in a thermostat at 370 (with chlorofDrm as
an antiseptic). Table I shows the results: (1) The higher the ion
strength of the buffer, the lower is the absotption of the RNA so-
lution. This phenomenon was fully reversible and was by no means
linked to the decomposition or denaturation of RNA. lafective-power
and high molecular weight remained unchanged. (2) Between PH 5.4
and 7.4 no absorption changes take place after 20 days of incubation,
this phenomenon being independent of the ion strength of the solvent
Thus the ribopolynucleotide is stable over this PH range. The loss
in infective power is not accompanied by any noticeable changes in
ultraviolet absorption. (3) On an incubation between PH 5-0 and 8.5
a certain tendency towards an absorption increase emerges. This RNA
instability is marked only in solutions of high ion strengthoA,11).
With PH 9.0, and even more so with PH 9.5, RNA is progressively de-
composed at any ion strength. It can be concluded from the experi-
ments that the spontaneous loss in infective power, on the 2nd-4th
days of incubation, does not in any way affect the absorption value
of RNA. Probably this process is not a consequence of the instabili-
Card 314 ty of the inter-nucleotide bonds.-There are 1 table and
references.

SOV/20-124-4-59/67
Spectrophotometric Study of the Effects of PH and Ion Strength on the Stability
of Higher Polymer Ribonucleic Acid in Solution
ASSOCIATION: Institut biokhiniii im. A. N. Bakha Akademii nauk SSSR
(Institute of Biochemistry imeni A. N. Bakh of the Academl of
Sciences, USSR) Moskowkiy gosudarstvennyy universitet imeni
M.V. Lomonosov (Moscow State University imeni.
M.V. Lamoaosov)
SliaMITTED! October 25, 1958
Card 4/4

170)
AUTHORS:
Spirin, A. S., Gavrilova, L. P., SOV/20-125-3-55/63
____1Te-1-o--z-`&_r__sY1`y, A. N., Corresponding Member, AS USSR
TITLE: On the Problem of the Macromolecular Structure of Native
High-pplymeric Ribonucleic Acid in Solution (K voprosu o
makromolekulyarnoy strukture nativnoy vysokopblimernoy
ribonukleinovoy kisloty v rastvore)
PERIODICAL: Doklady Akademii nauk SSSR, 1950, Vol 125, Nr 3, pp 658-661
(USSR)
ABSTRACT: There are but very few data in publications concerning the
problem mentioned in the title, despite considerable achieve-
ments in the study of desoxy ribonucleic acid (DNA). This is
explained by the exceptional difficulty confronting the
production of~the acid mentioned in the title (RNA). At an
earlier time, the authors reproduced (Ref 3) the experiments
made by other scientists (Refs 1, 2) and in the present paper
they describe the further progress made in the field under
discussion (Refs 4-11). Figure I illustrates data on the
comparative spectropho.tometric titration of the native
Card 1/3 (infectious) RNA and of a denaturated DNA of the tobacco

On the Problem of the Macromolecular Structure of SOV/20-125-3-55/63
Native High-polymeric Ribonucleic Acid in Solution
mosaic virus. The intensification value of the absorption of
ultraviolet rays by nucleic acids serves as a direct measure of
the number of burst hydrogen bonds (Refs 7, gy-10), It may be
seen from figure 1 that in the case of the native DIJA no
important variations of the said values occur in the entire
pH range from 7 to 3. Only at PH