SCIENTIFIC ABSTRACT ZHUKOV-VEREZHNIKOV, N.N. - ZHUKOV-VEREZHNIKOV, N.N.

• Document Type: CREST
• Collection: Scientific Abstracts
• Document Number (FOIA) /ESDN (CREST): CIA-RDP86-00513R002064930002-5
• Release Decision: RIF
• Original Classification: S
• Document Page Count: 44
• Document Creation Date: November 2, 2016
• Document Release Date: September 19, 2001
• Sequence Number: 2
• Publication Date: December 31, 1967
• Content Type: SCIENTIFIC ABSTRACT

S/560/61/000/011/007/014
E027/E635
'the results of the.
unfavourable factors, in, comparison with control matorials which
remained in the laboratory over the same period. In experiments
with bacteria 2m1. samples of suspensions of Escherichia coli,
Aerobacter acrogenes,. Staphylococcus auraus and Clostridium
butyricum containing 500 million organisms or spores per ml. were
sealed in ampoules, and exposed to a space flight of unstated
duration; the number of, viable individuals after the exposure
not differ si-nificantly froi-ii the values for the control
samples. A similar experiment was carried out witb the T2
phage of S. coli and the 1321 Pliage of A. acrogenes, w1tich were
r-ent in the second satellite; again, no sigificant reduction in.
the titre of the phage preparations could be detected after
return from space. Similar.results were obtained with
preparations of phaZe sent into space in the fourth and fifth
.Two bottles and six tubes of HeLa calls, some-of
satellites. U
which acre saturated with oxygen,, were exposed to space flight
Card 2/5

S/560/61/ooo/oll/007/012
E027/E635
The results of the
conditions, after it had first been shown that vibration and
acceleration did not detach the cells from the glass. The
cultures without oxygen appeared normal on return, whereas in
..those exposed to oxygen 1110st of the cells had degenerated.
.Subculture showed that 9051 of the cells, whea-r detached from or
remaining on the glass, were dead; however, two tubes gave good
growth, and the calls which grew up showed no abnormalities of
morphology. No antigenic differances could be datoctod in the
cells in anaphylaxis and deaensitization experiments in guinea-
Pigs. In subsequent space.flights fibroblast and human amnion cell
cultures were dudied, with similar results. Pieces of human and
rabbit skin were also used., On August 12th 1960 two pieces of
skin 2.5 x 3.5 cm. in size and 0.5 ",Im. thick were taken from a
human donor, placed iri Hanks solution and sent into space in the
second satellite. On recovery they were regrafted on the original
site.in the donor and became firml'y attached after seven days.
Card 3/5

S/560/61/000/011/007/012
The results of.the E027/E635
similar results were obtahed with two other donors. An npparatus
was devised for making a subculture in space, in order to study
the ability of bacteria to multiply under space conditions. In
experiments with Glostridium butylicuin no ddviations froat the
controls were observed. The second part of the woric wat; devoted
~to a study of possible genetic effects brought about by exposure
to space conditions, mainly by looRing for the production of
auxotrophic mutants and lysogeny in bactoria, The former svero
detected by inoculation on a layer of minimal medium irhich was then
covered'with an overlay of the same medium in order to fix the
colonies. When the latter had grown up their position was noted
and an overlay of complete mediLim was then put on, and the
colonies which then grew up as a result of the diffusion of
essentialnutrients were selected as wxotrophic mutants. No such
mutants could be found in suspensions of Escherichia coli
recovered from the second satellite. The exporipients on the
induction of lysogenic bacteria were carried out on a strain of
E. coli lysogenized by a X phage which had been exposed to cosmic
Card V 5

S/56o/61/ooo/oll/007/012
The results of the --- E027/E635
radiation in the fifth satellite. Free phage particles were'
removed by adding phage antiserum; after the end of t's-ke Intent
period the action of the antiserum, was cut short by dilliting
1.100'streptomycin was added to inhibit the host organism.4, and
the mixture was plated out on tho indicator strain in order to
count the phage particles, produced. The results obtained,
considered in comparison with control experiments, provided no
evidence of induction by cosmic radiation during a space flight
of ninety minutes. No difference was observed in the plaque
morphology. No.changes could be detected in the chemical and
physical properties of calf thymus deax7ribonucleic acid recovered
after a space flight. The results as a whole indicate that no
damage was suffered by isolated cells during a brief exposure to
space conditions. There are 6 figures and 10 tables.
SUBMITTED: May 23, 1961
Card 5/5

21739
S/02.5/61/000/006/001./007
D244/D305
AUTHOR: Zhukov-Verezhnikov, N. N., Member
TITIE: The first-journey..to-the.-stars.and problems-of space
biology
PERIODICAL: Nauka,i_zhizn!,_no.,6,.196l, 6-7
TEXT: This-article-briefly-disc~ugses-a..problem.encountered in
,man's.first-space,.flight-determining.-the.-influence-.of.various
cosmic- f actors...on.. the -organisms, of -.the...cosmanaut-. - -As. a first
step,.- re se arch-on.the.. effect.. of -overloading, -vibrations.; -ionizing 4
radiation,-.etc.-was..carried-out.on..biological-objects with a
,varying..degree.of..sensitivity.-which.were-placed,in orbiting.sput-
niksl...and..it..is-now,,possible-to-classify-these.items.according to
the.. levels.. of.. their. structural-physiological -organization.. E~.-peri-
ments-with-dogs,Layka, Belka, Strelka,.Chernusl-ata- and Zvezdochka
showed-that living creatures,are.able-to endure.nafely all the
peripetia..of-space,-travel 'in-a-hermetically~-scaled-capsule.
Other-aims-were-also-pursued by-sending guineapigs,white mice
Card 1/3

21739
S/025/61/000/006/001/007
D244/D305
The-first journeyoot
and.fruit flies.into-interplanetary space, the.former being
employed,for.studying.non-suseeptibility,-to.different external
influences..and-the.latter..,for.genetic-purposes..,.The second group
of.biological,.specimens which..included.skin.fragments-.were.studied
on-.the-le7z6l..of.their.tissue-orga'nization;.live.tiBsue.appears to
be-.most-suacept:Lble..to.the..effect.of-various-flight-factors in
cosmic space.. Additional..objects..such.as-cells-were.also sent
into the--cosmos, being,nurtured.in a.culture,away from the parent
organivm$ . fr=.whi-;h_ .they wer6 - rdrdovdd. , Gella Asiolated f rom a
.cancerous..-tumor-propagate.-well.under,such.conditious;.they can be
cultivated.on.glass.beneath a nutritious medium.containing the
same organic substances that are.supplied to the cells of complete
organisms by blood-and lymph. Bacteria and microscopic fungi
were.also-utilized.in..these tests, since,.being unicellular, they
serve-as-fine specimens-for.studying the behavior of cells in
space. It was foiind.that,bacteria.capable.of producing.bacterio-
Card 2/3

OUKOV-VEREUNIKOVp N.Nf; MAYSKIYp I.N.; PEKHOV, A.P,j RFEDIEVAp NOP,
Space microbiology. Mikrobiologiia 30 no.5t809-817 S-0 161.
(MIRA Us 12)
1. Institut eksperimentallnoy biologii ANN SSSR
(SPACE 141CROBIOLOGT~

--,-.ZHUKOVjMzlwl
Pv~ H91.4j, FAalovy A.Fe-f-BUKKO) leO-A-1,
Nature ai~d significance of bacteriophdge.. Report 110.3: Ultra-thin
phage soctions (methods and preliminary data). Biul. eks biol. i
Med- 52 ~o.10:78-79 0 '6i- 04~'U 15: 1)
1. Iz otdela immunobiologii (zav..- doystvitelinyy chlen AU SSSH
N.N.Zhul,.ov-Verozhnikov) Institute eksperimentallnoy bioloCii (dir.
prof. I.N.Yay:3kiy) -AMN SSSR, Moskva.

ABELEVp G.L., kand. mod. nauk; BUYRINSKAYA, A.G.,, kand. med. nauk;
GELITSERP R.R., prof.; GOLINMCH, To.M., prof.; ZHDANOV, V.M.,
prof.; ZDM)DOVSKIY, P.F., prof.; KALINA, G.P., prof.; rAULEN,
D.R., kand. mod. pauk; KIXTE)".0, V.S., prof.; KRYLOVA, O.P.,.
kand. med. nauk; KUCHEROKO, V.D., kand. med. nauk; LOMAKIN,
M.S., kand. med. nauk; MDSING, G.S., doktor med. nauk;,.PERSHINA,
Z.G., kand. sellkhoz. nauk; PEKFOV, A.P.., doktor biol. nauk;
PESHKOV, M.A., prof.; TIKHONEKKO, T.I., kand. mad. nauk;
TOVARNITSKIY, V.I., prof.1 SHEN, RA,, prof.; ETINGOF, R.N.j
kand. med..naukj KALININA, G.P., prof,, nauchnyy red. toma;
ZHUKOV-VEREZHNIKOV, N Y-P-rof.
,. - , otv, red.; YYGODCHIKOV., G.V.,,
prof., zameat. otv. red.; TIMAKOV, V.D., prof*, zam, otv6 red,
BAHOYANp O.A., prof., red.; KALINA, G.P.,, red.; PETROVA, N.K.9
tokhn. red.
[Multivolume manual on the microbiology, clinic,, and epidemiology
of infectious diseaseelMhogotomnoe rukovodstvo pa mikrobiologii
klinike i epidemiologii infektsionnvkh boloznei. Movkvaq Medgizi
Vol.2. (Generol microbiology,)Obsh,~,haia mikrobiolo in. Red. V.M.
.Zhdanov. 1962. 535 P. f9RA 16: 1)

BIJGROVAA Me) kand. med, nauk; VINOGRADOVA, LNVJ kand.biol. nauk-
DITAKGVY S-1-p kand, Mod. nauk; ZHDRIOV, V.M.p prof.;
ZHU
TU~KOV- ~UOV-r-N-N.j* Prof.; WfrSOVA, OqM*, kand,
ii-de nauk; IMSHENETSKIY, A.A.0 prof.; KALINA, G.P.) prof.;
KAULENp D.R., kand. med. nauk; KOVALEVA, A.I,; doktor mod,
nauk; KRASILINIKOV, N.A.# prof.; KUDLAY, D.G.9 doktor biol.
nauk; LELMEVA, M.N., prof.; PERMS, L.G.) prof. (deceased];
FWOVy A.F., doktor biol. nauk; PWIEL'YES, Kh.Kh,j prof#;
POGLAZOVA,, M.N., kand. biol. nauk; PROZOROV A A ; 1;121ITSKIY
A.A.j prof.; FEDOROV, M.V.t.prof. [deceasedl; F11WUTZ
V.Ly kand.biol. nauk; VYGODCHIKOV, G.V.p prof.t zamestitell
otv. red.; ADO, A.D., prof., rod.; HARMAll, O.A., prof., red.;
BILIBIN) A,F.p profep red.; BOLDYREV, T.19., prof,p red.j
VASHKOVj V.I.# doktor med. nauk, red.; VYAZOV, O.Ye., doktor
medo naukj red.; GAUZE, GoF#j prof6, red.; GOSTEV, V.S., prof.,
red:; GORIWNTOV, P.D,j prof., red,; CRINBAUMj F,,Toj prof4b
red Ideceased); GROMASHEVSKIYp L.V., prof., red.1 YELKIN, 1.1.1
proZ.., red.; ZASUKM , L.K,, doktor biols nauko redi;
ZDRODOVSKlYj F.F., prof., red.; KAPICHNIKOV; M.1441 kand. medo
naukp red.j KLEMPARMWA, N.N.j, prof., red.; KOSYAKCV, P.N.,,
prof., red.; LOZOVSKAYA, Ye,S., kand, mede nauk) red.;
MAYSKIY' prof., red.; I-11JR01-ITSEV, S.H., prof., red.
[deceasedfill. (continued on next card)

PuGhoAi V* 1. - (continued)- - Card 2,:
NIMIN M.Ya... red. NIKOLAYEVA, LAO,, red.; PAVIOVSKIY Ye*Nov
akademikp red.; PASTUKHOV, A.P.p kand. rod, nauk, red.;
PETRISHCIXVA, P.A,p prof.,:rod.;. FOIaWVSWA, HsP,j prof.,
red.; POPOV, I.S.I: kands medi.naukp red.; ROGOZIN, I.I.v prof.
red.; RUDNEVf G.P.,.prof., red.; SERGIYEV, P.G., prof., red.;
SKRYABIN, K.I., akad., red;; SON01My 14*1., prof. redy.1
_,SOWV'YEV,.V.D., prof., red.,
TRILDIEV. C.P,, dotamt, red.;
CHUMAKOV, M.P.0, prof.p red.; SHATRO7, W;of.., red.;
TIMAKOV, Y&-D,P *of&,# red.totaa; TROITSIV, VOL, praf., red.
toma;' MOVA, N.K.,, tekhn.red.j
lyfultivolume ma.nua.l:-on the microbiology clinical aspects,
and epidemiology of infectious disoasesi 1-inogotomnoe rukavod-.
--------------,------------stvo-po-mikrobiologii-k:Liniko-i-ep~demiologii-ing~ektsionrwkh
boleznei*- Otv, red-,-- 14 ON. Zhukov-VerezIuIikov.- -Moskva- Medgiz.
'Voll.- (General'microbiology]-bbshchaia. mikrobiologiia
red._N.14*Zhukov-Verezhnikov. 1962. 730 p. NIRA 15:4)
chlen Akademii meditsinskikh nauk SSSIt (for
Zhdanov,,Zbukov-Verezhnikovp-Vygodchikovp Bilibin,.Vashlkovp
Gromashavskiy., Zdrodovskiy, Rudnev~ Sergiyev, Chimakov,
Timakovo Troitskiy).
(Continued on next card)

.3 Problemy kos:il-clle, .- ', - L ! -, .4 -' - - ~- - 1 - I . -) y -Y
.
- I I.F . I I?6j2 . 26 -28.'+
N' !' . '~j. sak-van . "Os - o 7 -! - V,-, A, "~ 9 ~' ; P . I
I
4-

17, 11 41
TM W
ZHUKOV-VEREZ n'13, H U.; MAYSKIYp I.N.- YAZDOVSKIY
------- RYBAKOV, N.J.; KLMARSKAyA V-I.; FEKHOV, A.P.;
P N.N.; GYURDZHIAN, A.A.; TRIBULEV,
G.P.; NEFFD'yZVA1~N.p.; KApICHNIKOV, M.H.; PODOpUL
ANTIPOV - OV,
V-V-;'N0VIK0VA,-I.S.; KOP'YEV, V.Ya.
Problems of space microbiology and cytology. Probl.kosm.biol.
1:118-136 162. (MIRA 15:12)
(SPACE MICROBIOLOGY) (CYTOLOGY)

ZHUKOv- ;-MAYSKIY, I4N.; YAZDOVSX1Y, V.I.; YMOV, A*P*;
GYURDZHIAN, A.A.; RYBAKOV, N.I.; ANTIFOVP V.V.
Microbiological and cytological studies in spaceships. Probl.
ksom.biol. W40-148 162 (KM 16 4)
jSPACE BIOLOGY)

lWaRaIM V `I V; q a 1104 '10.VVVVI EI'V71"Ur .1,0 S,
40925
(025/62/000/009/001/002
D2
;Z,7.+Ooo 68/D307
AUTHORSs ;hu_kQY_-Y4r21bA1kAx,,~~t Professort Active
Member of the AMN OSSR and KOp1y8Tt-V-.Y&,9
Docent
TITLEt Biology and cosmic flighis
PERIODICALt Nauka i zhiznlt noo,gt 19629 15 19
TEXTs Biological conditions of planetary flight are
discussed..in general terms with special-reference to the danger of
contamination of the earth by pathogenic micro-organisms introduced
by returning ships. As a first stop in preventionp study of the our-
face of the planets by automatic biological*probse is advocated. In
this context bioslements of the-Academy of Medical Sdiences capable
of automatic recording of the reproduction of nicro-organisme and
the' transmission of appropriate signals to earth were carried by the
second Soviet space shipo They.consisted of a vessel# usually a metal
cylinder# divided into 2 chambers bya glass nembraneq one containing
sports of-butyrio fermentation micro-organisasp and the other ratrient
Card 1/2

S~025/62/000/009/001/092
Biology,and cosmic flights B266/D307
medium. Spores are sown to the medium when the membrane in broken by
earth signal or a programming deviwon the rocket. Reproduction is
accompanied by the formation of g&8*O-_'o*&tIvkg a pressure transmitter
through which a signal is sent to earth by rediotolonetrio apparatus.
These or similar bioelements could be used to d*teraino experikentally
whether life is,possible under conditions of long-distance flight at
velocities approaching that of light with - prolonged exposure to acce-
1*ration. Thojv.aight also.be used Ior Uologiaal verification of, the
theory of r6letivityo There are to.
--card 2/2

ZHUKOV-VEMM1IKOVj N.N.; PEKHOV, A.F.
Amtomy of phages and some problems of genetics. Vest.AWT SSSR,17 n0.3;
.37-48 162, (MIRA 15:4)
1, Institut eksperimontal,'noy biologiJ. M1 SSSR.
(UCTERIOPHAGE) (NU%ZIC ACIDS)

ZHUK.CV-VEREZHNIKOV,-, -N.H.; MAYSKIY, I.N.; FMOV, A.P.; TRIBUIEV, G.P.;
-RYMEV, I.N.1 RYBAXOVAp K.D.
Importance of microbiological objects in. he study of
pathological changes in genetic coding. st.Mal 8.8.8,R.
17 no.l2t49-59 062. ~hi (MIRA 16:4)
1. Institut eksper-imentallnoy biologii AMN S R.
(MICROMGANISMS) (OMTICS)

yevich, prof.j PE-KHOV, Aleksandr
ZHUKOV-VEREZflNI~Hx--Nlk
,91-v Nikolp:
red.; MIROBOVA, A.M...
tekhn, red.
[Genetics of bacteria] Genetika baktarii. Moskva, Medgiz,
1963. 1+57 p. (MIRA 16:12)
1. Daystviteltrqy chlon AMN SSM (for Zhukov-VerezhuAov).
(MMICS) (BAMERIA)

S'/0000/631 000/00010185/0188
'ACCESSION NR: AT4042681
,AUTHOR: Zhukov -Verezhnikov* N. N.: Mayskiy, 1. N. Yazdovskiy, V. L;
Pekhov, A Ry*bakdv, K.,_l Tribulev, G. P. ; Saksonov, P. P.; DobrovA
N. ; An4pov, V. V. ; Koz1ov, 'V. A.'; Vy*sots1ciy, V. G.; Mishenko, B. A.
Ry*bakova, D. Parfenov, G. P.; Pantyukhova, V. Vi: Yudin, Ye. V.;
'Aniskin, Ye.
-TITLE: The. evaluation of the biological effectiveness of space-flight factors
,:with the aid of lysoge-nic bacteria
'SOURCE: Konferentsiya po aviatsionnoy i kosmicheskoy meditsine, 1963.
~;:Aviatsionnaya i kosmicheskaya meditsina (Aviation and space medicine);
~.materialy* konferentsii. Moscow, 1963, 185-188
=PIC. TAGS: lysogenic bacteria- biological sensor, radiation detector,
..bacteriophage, phage, vibration, irradiation/ Vostok III, Vostok IV
'ABSTRACT: Lyadgenic bacteria, E. coli X-12 was carried on spaceships,

Mill PoftY?
2
ACCESSION NR: AT4042681
.,:..Yostok III and Vostok IV as a, biological sensor, The advantages of lysogenic
bacteria as biological sensors stem not only from their extreme sensitivity
to various types of radiation, but also from the fact that induced changes are
directly proportional to the dose o! irradiation. In addition, *E. coli was sub-
,.,.ijected,to the combined effects of radiation and vibration in ground experiments-'.,;,.--
-,Yibration was produced by m6ans of a vibrator with frequencies of 36, 70, and. -
~'700 cps, an amplitude ranging from 0. 4 to 0. 005 mm with a load equal to 10 go.
for periods of 15, 30, and 60 min. Co60 in doses of 100 r at a rate 'of. 21 r'
~er min served as a source of radiation. Lysogenic bacteria carried on space-',
ships Vostok III and Vostok IV revealed induction of genetic changes produced
by space-flight. factors which was indicated by a significant increase in the
number of.,phage particles. The induced effect was more pronounced on
-Vastok III than on Vostok IV. Forty-eight hours after Its return to earth, the,-
bacteria carried by Vostok III had produced 4. 6 times as many phage partlcles~
as cont 'rols which had remained on earth. Ground experiments with vibration
indicate that the combined vibration,and 'ganuna irradiation, followed by a
second exposure'to vibration, double the biological effectiveness of gamma rays.
rd 2/3
-------------- - ---------- ----- -

ACCESSION NR: AT4042681
Howe A
ver; when the bacteria is subjected to only a single dose of vibration
following irradiation, there is no increase in the number of phage particles as
compaFgd to samples which were exposed to irradiation alone. This fact
indicates, that under space flight 'c.--iditions vibration sensitizes the lysogenic
bacteria to, the effect of ionizing radiation. This as yet hypothetical explan
ation be substantiated by'additional experiments.
ASSOCIATION: none
~'SUBM'ITTED: '27Sep63 ENCL: 00 SUB CODE- LS
Nof RE;F!: SOV: 000 OTHE R: 000

-AGGESSION HR AT4o37688 S/2865/64/003/000/0184/0192
OR: rezhn1kovj,_K..-N.j Yazdovokiy, V, Lj Myskiy, 1, He; Tribulev*
"'Po,
-Pekht)vv A.P.; Saksonov, P,P,l Ry*bakov, N. I.; Antipov, V. V.1 Artenlyawt
--N6$.; Kozl(t)v, V. A.; Miahcheakos Be A.1 Yudins Yee Vol ft*bakoval K.D.;
I..-Aniskin Yeo D.
"ZITLE: Miorobiological and cytological research in the conquest of apace
1-=URCS:, AIR SSSR. Otdeleniya.biologichaskikh nauk. Problemy* kowdcheskoy
--b-i1dlogii' ve 3, 1964j M-192
TOPIC TAGS-1 n
microbiology, cytology, lysoge io bacteria, synchrocycl-otron,
:~-Ej6loitronq telemetryt space flight, antiradiation drug, ionizing radiation
jAHSTRACT: Microbiological research has concentrated on highly radiouensitive blo-
$logical~objecte which'regiater molecular changes in response to irradiation* Tb*:,i,'-
'~,spddific objenc selected was lyaogenic bacteria, 9 - cd"~ K-12 (A), which is very
Hiseneitive to ionizing radiation and reacts by producing phage,,particlesol Ment
1; aynQhrocyclatron experiments have shown that E.;~4U bacteria roact, similarly to
protona and neutrons and that the phage production is proportional to the irradia,
.'Card

ACCESSION NR: AT4037688
ilition dose. Other experiments have shown that when subjected to vibrationi
il-Ilysogenic bacteria do not produce phage particles. The value of this lysogenic
-11;system stems from the fact,that~it is highly sepsitive to radiation but stable
Runder other stress factors,'
,of space flight; In ~he immediate future it will be
necessary to couple this biological radiation sensor with an automatic system which
will permit registration "d telemetry of info4ation from space to earth. The
1principles for creating such an automatic telemetry system have already been work-
ad out, and thin makes it possible to begin conitruction of ex;erimental equipmente
Apparently, this lysogenic:aystem can also'be uged for tenting the effectiveneas
iof antiradiation drugs* Recent experiments with 0-marcaptopropylanint have shown
that phagb production can be reduced by the use of ouch drugs. If It turns out
radia-;
',!that phage production induced by heavy particles can also be reduced by anti
Ition drugs, then the lysogenic system could be used for a fast'pri=ary selection.
of new means of chemical protaztion against radiation.
;'ASSOCIATIONt none

7 Z"
twon"I Sit I i I I ml I ml p I E I I I III il i 114 1111 1!1 ils I I"[-, a

eta,

ALEKSAINAN, A.B., prof.; BEZDEIEZfINYKII I.S.) doktor vied. nauk;
flMAKOV, V.D., daktor med. nauk; BEM.,ERTNYY, H.S.0 dokt.
med. nauk; VASHKOV, V.I.p prof.; GROMASMSKIY, L.V.,
prof.,- YELKIN, I.I.j, prof.,- ZHDANOV, V.14.,, prof.;
ZFXOEVA, Z.M., kand. biol. nauk; KOVARSKIY, M.S., kand.
med. nauk; NABOKOV, V.A., prof.; NOVOGORODSFAYA, E.M.0
prof.; PAVLOVSKIY, Ye.N.j. akademik; PETRISHCHEVA, P.A.,
prof.; PERVOMAYSKIY, G.S., prof.; FOGODINA, L.14.1 ROCOZIN,
I.I., prof.; SUKHOVA, M.N.p doktor biol. nauk; CHASOVNIKOV,
A.A., kand. vied. nauk; SHATROV, I.I.y prof.; SHURABURA,
B.L.p prof.j YASIIKULI, V.K., kand. med. nauk;
prof.,, otv. red.; FJOLDYRE
-ZUWLOV-Y&jEZRjj 'Vp T.I.)
prof.0 red.; ZASUKHIN, D.N.p doktor biol. nauk, red.;
MINA) G.P.p red.
(Multivolume manual mi the microbiology, clinical aspects
and epidemiology of communicable diseases] mnogotomnoe ru-
kovadstvo po, mikrobiologii,, klinike i epidemiologii Infek-
tsionnykh boleznoi. Moskva.. Meditsira. Vol.5. 1965.
548 P. (MIRA 19:3)
1. Deystvitellnyy chlen PEN SSSR (for AlcksanyF.-n,
Gromashevskiy~ Zhdanov, Zhukov-VerezbnIkov). 2. 7hler-
korrespondont A14N SSSR (for Rogooin, 13oldyrev).

L 1424L-66 FSS-Orkri M/Ey !F Or /~V1A(d)/T/z-,%%(b)-2 SCTB TT/bD/jK)~Rb~~_
M U 31ZEC00-2
-ACC - NR: AT6003S_ 0. SOMM CODE. UR/2865/0/0%/Opo/
AUTHOR: Zhuko~-Verezhnikov. N# U;,,,, Rybak t Lb Q014 _Y _-J Sa P P
gy, N._ I
vit -v
r
..Dobrovb. N_._W.T,!!~T01P0 ~74 p6d*p P~Kfqn v t.
_f J
nona -
nduc, R-711
Results of rAier ogical: aifd~
ights -"Vostok" tnn,vehicles
during the fl
SOURCE: MJ SSSR. Otdaleniye lijologict-te skikh nauk. Problemy kosilicheskoy biologii,:
965, 261-269
4
. I
TOPIC TAGS: bacteria netics, bacterial genetics# gamma irradiation, cobq~Lt#.
1 913
radioisotope, microbiology, cytology, spz,!ce biologic experiment, radiation
biologic effect, biologic vibration effect
ABSTRACT: The biological objects used for space research are carefully selected
genetic indicators. E. coli K- 12 ( ~, ), "requently chosen for 1hese experi-
ments, is a reliable biological dosimet~-r nf the genetic effectiveness of
spaceflight factors. When normal and -ane erous hurnar, rells were exposed
in the Vostok series, It was found that these experimental samples did not
differ essentially from control samples kept on earth However, some
tendency to intensification of phage production was observed in cultures
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of E. coll in this series (an increase bya factor of 1. 2 on Vostok- 2~
4. 6 on Vostok- 3, iand 1. 96 on Vostok- 4).'~ Data !rom repeated exposi
of the same biological object indicate accumulation of the spaceflight effect,
although the character of thi accumulation is not clear. In a comparison
of the results of Vostoks 3 1~
-6, it was not possible to establ"Ish a linear
dependence of biological effect on tinie of e)cposure in space. However,
factors r-ausing a genetic effect (an in-t ease in the ph age -producing activity
of a lvsoeienic culture) definitely oDerated riurina these t'lights.
The following derived values of indured phage production were calcu-
lated- -~, 3 for Vostoks 3 and 5 (correspondm~y tn the Inducing effect of 3. 2
rad of gamma-rays), and 1. 8 for Vastt)kq 4 an,! F, (corresponding to 0. 8 rad
of garnma-rays). Since the doses quoted are higher than those eneoun,.ered
in spaceflight, the obse.
genetic effect mu t therefore he parTially due
to other factors (such as weightlessnei3s, acceleration, vibration, etc.
To study the operation of one of these factors, I-. coli K-12 was S; ub-
jected to vibrations of 18, 15, 75, 100, and 700 cps for 15- 30 min. and,
in another series of experiments, to vibration in combination with Co"
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garnma- irradiation (dose, 100 rad; dose power, 21 rad/min), T'he experi-
mental results show that vibration alone does nof Induce phage production
but does increase the sensitiVity of lysogenic bacteria to the subsequent
influence of gamma- irradiation. It is ;uggested that vibraticn helps senst-
tize cells of a lysoge-nic culture to 'he influerice ~-( cosm,c radiatior, a'thourh
it ts also possible that the cause of ~,YeT,etia: -Ganges is 6eiKh,lessne_55 r,
combinatiM with radiation. ,Drig. ar--. nas;
[ATD MSS: 4091-F)
SUB CODE: 06 SUBM DATE: none ORIG REF- 009 OTF REF: 002
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L 14295,--g EWTW/EPF(n)-2 _GG/RD
ACC PIR: AT6003878 SCURCE CODEE: UR/2865/6-1004/000/0445/0450
AUTHOR: ZhukqY_7Verqzhnikay._ N. N.; Volkov, M. I.; 4jtakov I.; Saksonov P. PIj
Koziov. V. A.; Kons V4. r,
tantinov F.
V. Dobro-,
ORG: none
TITLE: New ways of studying chemical pr tection against g
Inetij_atan e8
SOURCE: kN SSSR. Otdeleniye biologi~_-he,3kikn naik. ProLlem,7 ko3niclyeskoy
biolo~aii, v. 4, 1965, 4.45-450
TOPTC TAGS: bacteria, x ray irradiation, bacterial genetics. chemical agent
ABSTRACT: Aminothiols and some pyrtmidine analogs were tested for their ability
to block development of infectious phage from prophage after induction of
E. coli K- 12 ( A) with x-rays. Doses with a previously establiqhed non-
toxic effect (0. 05% concentration) were used. Tlie destre(i cnernical
preparation was added to a bacterial culture diluted in a physiological
medium. Experimental and control garnples were suh]ected to x-ray
irradiation (dose, 15, 000 r) and ther, cultured on agar. 'Fhe number of
-induced phage parti*cles Ln irradiated samples with and without each prepa-
ration was then compared. 2-Mercaptopropylamine hydrochloride was
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most effective-, cultures treated with it produced 119 times fewer phage
particles than control samples. Other good inhibitors of induced phage
formation were 2- (gamma- aminopropyl) disulfide dihydrobromide, sodium
diethyiditFioca.rbarnate and ammonium diihiocarbamate. wl-ich reduced
phage product-.on 76. 3-70. 1 times. Less effective were !he Salts ~,-,f a -
mercaptoethylanitne tested: 2-mercaptoethylarnine hydrohromide, 2-
rnercaptoethylamine disulfide hydrochloride, 2-mercaptoothYlamine
hvdroiodide, and 2-mercaptoethylamine hydrochloride.
The experlmental data show the essential connection between the
chemical structure of the tested preparNtions and their albixiity ~o blGck the
cL infectious- uh aae;- - -T-h e- aj ktivenetic effect of a -Inercanto,
eveloDment-
A
yth-eirbase, -It---may-he~~Vos:sible-to-bbtat PiFaffi(
ty-everymore effective pre
of this compo~ind by forming salts with other acids. The failure of
3- B -aminoetf-yllsothfuronfurn hydrobromide to produce an antivenettc
effect is espe-lally interesting because In previous experimenti this com-
pound decreased the death rate of animali subjected to a 'lethal radiation
dose by 70-100C Orig a2-t. has: I tab!Le . ~ A-D PRI-~~,,,;: 1,o914 -
SUB CODE: 06 3UBM DATE; none REF:
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tained in the second generation. However, preparation P-46 completely
removed the injurious radiation effect in that generation. ExT)erimental
data indicate the possibility of partially or completely removing the
depressing effect of 0-radiation or. plants with the help of phy(ifologically
active compounds. Orig. art. has: 4 'IaDLes. (ATI', FRZ-E,;:
SUB CODE: 06 SUBM DATE; none ORIG HH. 004 WH R H,: 005
(9e1
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-,r(l)/K -rB TT / D D NIK / t 7W
-L 37643-66 K)S-2/bw G(k)-2jFCCtr- Sr;
_ACC NRs AP602465o - SOURCE CODE:, UR/0216/66/ooo/oo4/O592/0593
AUTHOR: Zhukov-Verezh!jjkov No No-, Mayakiy, lo.N.;,Pekho , A* Po;
Vo; Vo Ao;
Rybakov, No ritilTov, V-8 _~ozlov,_
'Saksonov,_P. P.;_Eodoplelovt is 10
ORO: none
,TITLE: Results of study of the effect ofLcosmic radiation and other
spaceflight factors on lynoffenic bacterinVand human cell cultures
[Paper presented at the" Anniverea-r-y Symposium of the Institute of Bio-
physics of the Czechoslovak AcadSqX of Ecieqcee held in Brno in May
SOURCE: AN SSSR. Izvestiya. Seriya biologicheskaya, no. 4, 1966P
592-593
TOPIC TAGS: spaceflight effect, radiation effect, Hela cell, lyeogenic
bacteria / Vostok -spacecraft, Vostok 6 spacecraft, Voskhod 1 spacecraft
ABSTRACT: Sin le-layer cultures of normal human cello (fibroblasts and
amniotic cellel and human cancer cells (Hela strain), together with
cultures of lyoogenic bacteriA (E. coli~K-12), have been consistently
used as radiation indicato sll'n Soviet spacecraft, Results of these
r
experiments have shown thath7e~eated %xposure of a culluKe of Hela'cellj
to spaceflight.factora on t e , 0stok- and Vostok-6 fl g ts produced
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c hangee In experimental cells as empared with laboratory 1-ontrol s and with Hela
cells exposed on one spaceflight only*, A longer latent period of
recovery of growth capacity and other characteristics [not named) were
noted in tvice-flown cultures, In add ition, the coefficient of pro
liferation for Hela cells exposed on both Vootok-4 and Vostok-6 was
o.ne-half that for intact.controls and for Hela cells exposed to space-
,:rlight onl-.once,' These data suggest that spaceflight factors have
a cumulative bi6logical effect on human cell cultures. However
direct dependence of biological effect on length of spaceflight expo-
sure has not be-en established in experiments with the other radiation
indicator, the lynogenic bacteria E. coli K-12 (A). It is interesting
to note that when the same Hela cells used on Vostok-4 and Vostok-6
were also exposed on Vookhod-1, a veil-defined drop in.the prolifera-
tiofi coefficient was observed in comparison with intact cultures.
Experimenial colonies were more compact, and there vare more dead calls,
Other reliable x1ifferencee [not enumerated) were also found between
intact controls and thrice-exposed cultures, However, no reliable
dif f erences could be detected between thrice-exposed Hela cells and a control
strain used only on Vostok-6., It is',suggested that,the biological
effect of spaceflight may be the result-of the combined influence of
radiation, vibration, and veigh'tlessness.., (is]
SUB CODE: 061 SUBH DATE: none/ ATD PRESS: q0
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