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pproved For Release 2001/03/07 : CIA-RDP96-00792R000500240008-9 INTERACTIONS WITH SCALAR ENERGY- CELLULAR MECHANISMS OF ACTION Dr. Glen Rein U.S.A. Recent direct experimental data as well as scphisticated ccenputer-enhanced mathematical analyses of pre-existing data has revealed the predominance of non-linear processes in biological system s(1). These new findings are a dramatic departure fran traditional' theories which indicate that . biochemical reactions are at or near a State of equilibrium and that cells function in a lirear, dispersive and degenerative manner. Electrochemical oscillations between membrane-bound lipids near phase-transition temperatures have been reanalyzed with the aid of powerful computers and shown to be mast acurate1y described by nonlinear quantum rechanical equations (2). Similar analyses of ccupling betweenharmanto oscillators represented by actionixtentials generated fran active neuronal networks in the-centrea nervcusSystsm has revealed theirrxxl-linear nature(3). Several types of quasi-particles eachwiththeirown characteristic resonant frequencies have been Proposed to Mediate these nomainear phenamenon, imludirg solitons, excitons, and plasms. Biological solitons have received the most attention and have been postulated to be carriers of biolcgical information along maaccmolecules like.DNA(4) and alpha-helical intramembrane proteins involved with signal transduction medhanisms(5). Furthermore, solitons have been postulated to mediate the coherent electronic excitatials associated with the action of ultra-violet and visible light on, biolcgical systems(6) and the calcium-dependent cooperative interactions hetweeen extracellular glycoproteins on the surface of the plasma rembrane in response to weak ELF electromagnetic ON radiation(5). In light of the non-linear nature of biological systems, it .se likely that they should he highly sensitive' to non-linear for of acoustic and electrangneth:racliztion in the environment and that the right frequencies of non-linear energies might have profcund healing prcperties. Exogenous non-linear energies were first utilized by Nicholas Tesla in 1899 in his fameus Colorado Springs experiments where he demonstrated the transmission of his "Tesla waves" without .energy loss (7). Thwenen-Bertzian longitudinal waves have since been studied in: hydrodynamics geolcgy and astrophysics. The extraction of scalar couponents frau Maxwell's original e1ectrcoagneticequations(8,9) and frau Schrodinger's equations using imaginary nuMbers(10) has recently been reported. The unusual prcperties of these non-linear waves have also been 'described by ether investigators(8,11,12.). Homer, relatively little attention has been given to their effects on biological systems. Due to the non-linear nature of biolcgical systeuxs it was prppcsed that scalar waves should be more biologically active than their linear electranagnetic counterparts (13). A recent report by the author prcposed a new theory, the Crystalline Transduction Theory, to explain the =chant= of action of scalar waves with biolcgical systems. It was proposed that scalar energy is transduced into linear electromagnetic energy in the body by liquid crystals in the cell rembrane and solid crystals fcund in the blood and in several biological tissues. Direct effects of scalar energy and ccupling Approved For Release 2001/03/07 : CIA-RDP96-00792R000500240008-9 C Approved For Release 2001/03/07 : CIA-RDP96-00792R000500240008- of scalar energy to conventional electromagnetic fields was also The difficulty of cbtairdning reliable scalar generators for considered(14). biological studies has led to a paucity of bard experinental data.However, Dr. Andrea Pdharidh successfully adapted the ftoibius strip" technology for generating scalar waves to a watdh. ELF Cocoon Int. has recently modified and impoved Dr. Puharidh's original design and now na?ket a readily available scalar generator, the Tesla watdh. Despite the availability of sudh a scalar generator little is known &oat the redhanism of action of scalar energy with biological systems. Nureicus anecdotal clinical Observations of the healing properties of the Tesla watch have been reported. Since several of these reports include irzidences where dininution of the symptomology was correlated with cessation of the scalar emissions. from the watch (eg. expired batteries) without the knowledge of the subject, placebo effects are unlikely to explain the clinical results. Nonetheless, it is unknown whether the scalar fields directly effect diseased tissue in the body or 'whether the clinical effects are mediated through a direct effect of scalar energy on the brain/mind. If the braip/mind is involved it is likely that various psychological states(eg. stress, roods or beliefs) will alter the _biological effects of. the scalar energy. CLDNICALSMDIES WITH SCALAR .ENERGY These variables complicate interpretation of clinical experimentswith the scalar energy. In additon to conventional confounding variables, one rust also cons:Wei:the EM environment. These factors may explain why some individuals are rose sensitive to scalar energy than others, as has been previously cbservedwith EM energy. Even When individuals Who are sensitive to scalar energy are predetermined and isolated frown the rest of the population under study, they Show a highly variable response to treatment. These problems rake it difficult to study the healing properties of scalar (or EM) energies in a clinical setting. MITIMEITIMENUIENCE MR THE BIOLGICAL, EFFECTS OF SCAIAR ENERGY Dr.Eldon Byrd studyed the effects of scalar energy on brain waves by reasuring subjects EEG before and after wearing a watch(15). The results indicate an increased amplitude of all EEG frequencies with a preferential effect in the lower ELF frequencies. Inaddition' an exogenous fregency of 82 HZ was not seen in sdbjects wearing the watchindicating the scalar wave neutralized this EH signal in the environment, thereby protecting the individual. It is unknainlobether this altered brain state is the actual cause of clinical healing whidh scmetimeS occurs several months after initial exposure to the scalar waves. It is known that the brain and the body, eq. the immUne system, commmmioltAatairceAgnally(16). Thus, a direct action of scalar energy on the body could cause a subsequent Change In brain states. Therefore these EEG studies do not imidcate whether scalar energy also effects the body directly or whether the brain and the body are both effected. THE TISSUE CULTURE APPROACH -2- Approved For Release 2001/03/07 : CIA-RDP96-00792R000500240008-9 Approved For Release 2001/03/07 : CIA-RDP96-00792R000500240008-9 The use of cultured cells, separated from the body and therefore isolated (at least to a certain degree) from the influences of the rind offer a unique experimental approach to understanding the mechanism of action of scalar energy in the healing process. This approach could be used to determine direct effect of scalar energy on cells from either the brain or the body. Tissue culture cells are a stable controlable biological preparation which could also be used to study the biochemical medazulism of action of scalar energy. Even if scalar energy directly effects individual cells it may also effect the mind resulting in an altered psychological ochookidlich results in clinical improvement. Dr. Persivn7was the first person to test the effects of scalar energy using isolated cells (17). The scalar waves were generated by partially cancelling two vortex-type magnetic fields(0.5HZ, lOuT) by intersecting them in air. Although a scalar field is likely to be generated in this protocol, it was not measured. The biological endpoint, degranulation of mast cells, was increased by the scalar fields. It is unknown to what extent the EM fields contribute to this result. Very recently, Dr.PUharich has reported that the Scalar energy from the Tesla watch effects the DNA of E.Coli cells grown in tissue culture(18)4 WOrkinguith colleagues at the Max Plank Institute in Germany, they have _shoal that ..the P1D-6 gene is activated-by_scalar energy resulting in in=eased activity of ubiquitone. Ubiquitone is a protein involved in DNA repair. These results indicate that scalar energy can have a direct effect at the subcellular level as well as a direct effect on the immune system (nest cells). EFFECT OF SCALAR ENERGY ON mumairomummutrmalou $ - The present stuly is the first to reporta direct effect of scalar energy on nerve cells in tissue culture and indicates that scalar energy canmcdulate the basic biochemical ocaurdmtionketween nerve cells mediated by neurotransmitters. Nerve cells were chosen for to dab/amine whether scalar energy can directly effect the nervous' system in the absence of any feeMam7k signals frau the body. Cultured nerve cells offer a unique rodel to answer this question. Aparticular type of nerve cell, called PC12 cells originally isolated frcm a rat adrenal phaecchromccytana, was cells, because its neurotransmitter properties have been well characterized and Shown to be similar to. those occuring during normal synaptic transmission in the brain (19,20). Furthermore, the author has previously shaan that the functional I properties of noradrenaline, an abundent neurotransmitter in these cells is altered by weak EM fields. Noradrenaline release from PC12 cells was shown to be incraised by a 500Hz monopolar square wave(21) and naradrenaline uptake was inhibited by a 15 Hz bipolar square wave (22). Noradrenaline uptake was chosen as the functional neurotransmitter property for this study for several reasons. Neurotransmitter uptake is diret.lymediated by the plasma membrane via an intramembrane protein carrier. The plasma membrane, with its liquid-crystal strucute, is the critical cerlulzu7harrier which interfaces with the chemical and electromagnetic environment. Since other intramembrane proteins show non-linear properties the plasma rembrane appears to be a likely target for scalar energy. AlhIctxjh neurotransmitter release also involves the plasma membrane, it is a much rore complicated process which occurs several biochemical steps down the line from the first exposure of the membrane to the EM signal. -3- Approved For Release 2001/03/07 : CIA-RDP96-00792R000500240008-9 Approved For Release 2001/03/07 : CIA-RDP96-00792R000500240008-9 The physiological function of carmio:.meddated neurotransmitter uptake is to remove excess neurotransmitter from the synaptic cleft after its release from presynaptic nerves. Thus, inhibiting uptake results in the accunulation of the neurotransmitter in the synaptic cleft. This is the mechanism of action of tricyclic antidepressants, since they inhibit naradrenalins uptake.repression is associated with decreased noradrenalinelevels. Dar these studies, the same protoco 'hiCh was previously used to . determine the effects of electromagnetic fields on noradrenaline uptake was used(22). This involved exposing confluentronolayer culture of PC12 cells to radiolabelled noradrenaline in the presence or absence of scalar energy. After a 30 minute exposure period, the cells were washed and centrifuged according to standard biochemical procedures and the amount of noradrenaline that was taken up into the cp11s was neasured using a Beckman scintillation counter. The results: were normalized with respect to the total intracellular protein concentration as determined by standard biodhemical methods using.a spectrophotaneter. The final specific activity results are expressed as opm/mg pribtein. The Tesla wato41 obtained from ELF Cccoon,:Intenational was used as a source of scalar energy. According to Dr.Ptharictlthe scalar B field _Emitted by. the watoh is_around8.liz.lbeJesaawatch (digital.version) was placed directly over an uncoveredpetri dish containing confluent PC12 _ cells. At the same time a separate control dish, paaced about 12 feet frima the experimental dish, was exposed in an identical way to a watch that did not have the scalar generating mobius strip. In this way both control and experimental dishes received the same electrcaagnetic field exposure, but the experinental dishes were also exposed to a scalar field. The results are indicated in Table 1. Menial, uptake of noradrenaline into PC12 cells was inhibited by 19.5% in the presence of the Tesla watdh as compared'with the control value obtained in the absence of a scalar field. Standard t-test statistical analysis revealed a highly significant difference between control and experimental dishes of p 0.01. Table 1 EFFECT OF SCALAR ENERGY ON ECRADEENAL1NE: MARE TRIAL CONTROL EXPERIMENTAL (orElArg Protein) (creVng Protein) 10min 30min 10min 30min 1. 1111 4065 1079 3500 2. 880 7032 1137 5009 3. 1076 4520 1110 3592 4. 9033 6035 5. 4277 4041 6. 4117 Bean 1022 5507 1109 4435 + 72 +841 + 17 +480 These results allow us to conclude that the scalar energy has a direct effect on nerve cells. The inhibition of uptake is time dependent with no significant effect being observed after a 10 minute exposure. It is -4- Approved For Release 2001/03/07 : CIA-RDP96-00792R000500240008-9 ed For Release 2001/03/07 : CIA-RDP96-00792R000500240008-9 unknown whether eKcosure times greater than 30 minutes would produce a 1aer effect or whether the effect would be maintained either in the continued presence of the scalar field or not. Mese questions will be examined in additional everiments. Since inhibiticn of uptake occured after only 30 minutes, it is likely that this effect is an early biological response to scalar energy and ray be the trigger for subseciuent physiological changes that result in improvement of a wide variety of clinical diseases. Since the scalar energy inhibited the uptake process, it is acting like a traditional antidepressant drug, thereby explaining the antidepressant action of the Tesla watch experienced by sore t These results also indicate that scalar energy, like electranagnetic ?, energy, can have a direct effect on the cell membrane. Since both scalar and electrcragnetic energy have the- same effect on neurotransmitter r*? uptake, the results offer indirect support for the Crystalline Transduction theory. Thus it is possible that the scalar energy could be comrerted to electrceagnetic energy in the membrane and the latter actually cauzcz the biolcgical effect. 11.70.7ever it is also possible that the scalar energy will have a direct effect on the uptake system. Needless .to say in the constant presence of, electromagnetic fields in biolcgical it. will be _difficult to rule out .this possibility. However, certain experimental approaches could be used to determine the mechanism of action of the two types of energies. ?eg.determine whether alterations ln the liquid crystal structure in the membrane would change the effect of othe scalar energy on uptake. In light of the previous discussion, it is also possible that the scalar energy will effect other cells in the body in addition to nerve mils. The tissue culture approach will be an invaluable tool in determining to what extent this is true. This approach cculd also be used to find cut whether diseased cells are preferentially sensitive to scalar energy. The tissue culture approach is thereore a valuable method for studying the biological effects of scalar energy and for ultimately characterizing its mechanism of action. REFERE1XES 1)" Nonlinear Electrodynamics in Biological Systems", Adey,W.R., Lawrenoe, A.F.(eds.),Pleramt Press, N.Y. (1984). 2) Loosley-Millnan, al. Biophysics J. IQ 221 (1982) 3) Basar,E. American J.Physiol. 215 R510 (1983) 4) Barlow, al. Nature 222 312 (1988). 5) lawrence,A.F.,Adey,W.R. Neurolog.Res. A 115 (1982). ? 6) Bednar,J.Internat.J.Radiation.Biol. Ag 147 (1985). 7) Tesla,N."Colorado Springs Notes" NoLit,Belgrade,Terazije 27 ,Yugoslavia. 8) Aids: Biolcgical Warfare, Bearden,T.E.,Tesla Book O. ,Texas (1988) 9) Bass,R. Proc.Tesla Symposium,p89,Internat.Tes1a Soc.,Colorado Springs (1984) . 10)Rau.scher,E.A.,Technic Res.Lab Report PSRE.,-3107A,San Leoandro, CA. 11)Quantum Mechanics and Nonlinear Waves,Burt,P.B.,Harwcod Acad.N.Y. (1981) 12)Bearden,T.E. PACE Newletter 2 (4)p10(1982) . 13)E3y2XLE.A. 35th ACEMB Conf.Abstracts,p218,Sept. (1982). 14)Rein,G. In "Mechanisms of Psychic Perception",M.illay,J.,Sirag,S-P.(eds) in press. 15)Byrd,E.A. in ELF Cocoon Internat.Catalogue,St.Francisville,n. (1987). 16)"Psychoneuroirraunology", Ader,R.(ed.),Academic Press,N.Y. (1981) . ? Approved For Release 2001/03/07 : 6.51A-RDP96-00792R000500240008-9 Approved For Release 2001/03/07 : CIA-RDP96-00792R000500240008-9 17)see Byrd,E.A. Archaeus Jcurnal 1 (1)pl (1983). 18)Puharidh,A. 14th Ann.USPA Bioenergy,Dayton,Ohio (1988). 19)Greene,L.A.,Rein,G. Nature 20 349 (1977). 20)Greene,L.A.,1ein,G. Brain Research 233 521 (1977). 21)Dixey,1t,Rein,G. Nature 216. 253 (1982). 22)Pein,G.,et al.Proc.9th Ann.Bioelectranag.Soc.,Cregon (1987). -6- Approved For Release 2001/03/07 : CIA-RDP96-00792R000500240008-9