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December 28, 2022
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September 26, 2017
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March 25, 1986
PDF icon YELLOW RAIN-ANALYSIS EVAL[13984487].pdf256.13 KB
Approved for Release: 2015/01/05 000037506 9 Analytical Division Research Directorate 25 Mar 86 Analysis/Evaluation of Leaf Samples A shipment designated 10027 CCC consisting of two leaf $821 89 received by the Analytical Division from FSTC 1 Janua e first sample, designated 10027CC0-1 consisted of a large �rown leaf with yellow spots aud what appeared to be white mildew or fungal areas. (Fig 1). The second sample, designated 10027CCC-2 consisted of a smaller leaf with similar spots (Fig 2). Vapor samples withdrawn from within each sample enclosure were subjected to analysis by gas chromatography/mass spectrometry (GC/KS). A portion of each sample was extracted with chloroform. Another portion was extracted with 1:1 methanol: water. The solvent soluble materials were analyzed by GC/!LS, ion chromatography (IC), thin layer chromatography (TLC) and infrared spectrometry (IR). A portion of each leaf sample was subjected to scanning electron microscopy evaluation. The GC/MS spectra of the vapors associated with each of the leaf samples identified the presence of diphenylamine and a series of phthalates. Each of these compounds could be from the plastic containment bags, or be a dissemination component. The GC/MS spectra of the chloroform eolubles identified the presence of phthalates in each sample. IC detected no ions of interest. No detectable components were separated by TLC. IR identified the presence of water, aliphatic hydrocarbons, a carbonyl band at 1735 cm , and possible soil components. Derivatization with negative ion chemical ionization KS detection identified the possible presence of neosolaniol in sample 1 and T-2 tetraol in sample 2 at very low picagram levels. Outside laboratories were unable to confirm the presence of trichothecenes since their minimum detection level was higher than CRDEC's. SEX identified spores with an hypae overgrowth (Fig 3) and pollen, including an unusual triangular shaped pollen (tricolparate) (Fig 4) on sample 1. The same materials were identified on sample 2 in addition to a striated crystalline material resembling that of sample 10027 TY-1. Conclusion: No evidence of any CW agent or agent degradation product was detected. The unconfirmed detection of low levels (picagrams) of the trichotheeenes aeosolaniol and T-2 tetraol is of interest. The significance of possible trichotheceme detection combined with the Approved for Release: 2015/01/05 000037506 Approved for Release: 2015/01/05 00003506 presence of phthalates, and a striated crystalline structure, which could be dissemination components cannot be identified it this time. Approved for Release: 2015/01/05 000037506 Approved for Release: 2015/01/05 000037506 < � ; � 4. - � s � � -% - '� - .41 fr � ,$4:141' C' 1 , � ���iFt,L, � � � : .7--Approved for Release: 2015/01/05 C00037506-�� Approved for Release: 2015/01/05 000037506 ���11�11 FIGURE 3 SPORES WITH HYPHAE OVERGROWTH FIGURE 4 TRIANGULAR POLLEN Approved for Release: 2015/01/05 000037506